Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru
Leishmaniasis is a worldwide infectious parasitic disease caused by different species of protozoa of the genus Leishmania, which are transmitted to animals and humans through the bite of insects of the Psychodidae family. In the present work, the antileishmanial activity of an alkaloid extract of th...
- Autores:
-
Soto-Vásquez, Marilú Roxana
Alvarado-García, Paul Alan Arkin
Osorio, Edison H.
Tallini, Luciana R.
Bastida, Jaume
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Ibagué
- Repositorio:
- Repositorio Universidad de Ibagué
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unibague.edu.co:20.500.12313/3836
- Acceso en línea:
- https://hdl.handle.net/20.500.12313/3836
- Palabra clave:
- Alkaloids
Amaryllidaceae
Clinanthus milagroanthus
Leishmania braziliensis
Molecular docking
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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| dc.title.eng.fl_str_mv |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| title |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| spellingShingle |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru Alkaloids Amaryllidaceae Clinanthus milagroanthus Leishmania braziliensis Molecular docking |
| title_short |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| title_full |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| title_fullStr |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| title_full_unstemmed |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| title_sort |
Antileishmanial activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) collected in Peru |
| dc.creator.fl_str_mv |
Soto-Vásquez, Marilú Roxana Alvarado-García, Paul Alan Arkin Osorio, Edison H. Tallini, Luciana R. Bastida, Jaume |
| dc.contributor.author.none.fl_str_mv |
Soto-Vásquez, Marilú Roxana Alvarado-García, Paul Alan Arkin Osorio, Edison H. Tallini, Luciana R. Bastida, Jaume |
| dc.subject.proposal.eng.fl_str_mv |
Alkaloids Amaryllidaceae Clinanthus milagroanthus Leishmania braziliensis Molecular docking |
| topic |
Alkaloids Amaryllidaceae Clinanthus milagroanthus Leishmania braziliensis Molecular docking |
| description |
Leishmaniasis is a worldwide infectious parasitic disease caused by different species of protozoa of the genus Leishmania, which are transmitted to animals and humans through the bite of insects of the Psychodidae family. In the present work, the antileishmanial activity of an alkaloid extract of the bulbs of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) was evaluated in vitro, in vivo, and in silico against the parasite Leishmania braziliensis, and the chemical profile of the sample was determined by GC-MS analysis. At concentrations of 1, 10, and 100 µg·mL−1, the alkaloid extract presented inhibition percentages of 8.7%, 23.1%, and 98.8%, respectively, against L. braziliensis with a p < 0.05, and IC50 values of 18.5 ± 0.3 µg·mL−1. Furthermore, at a dose of 1.0 mg·kg−1, a greater decrease in lesion size was observed (90%) for in vivo assays, as well as a decrease in infection (96%), finding no significant differences (p > 0.05) in comparison with amphotericin B (92% and 98%, respectively). Eleven alkaloids were identified in C. milagroanthus bulbs: galanthamine, vittatine/crinine, 8-O-demethylmaritidine, anhydrolycorine, 11,12-dehydroanhydrolycorine, hippamine, lycorine, 2-hydroxyanhydrolycorine, 7-hydroxyclivonine, 2α-hydroxyhomolycorine, and 7-hydroxyclivonine isomer. A molecular model of Leishmania braziliensis trypanothione reductase (TRLb) was built using computational experiments to evaluate in silico the potential of the Amaryllidaceae alkaloid identified in C. milagroanthus toward this enzyme. The structures galanthamine, 7-hydroxyclivonine isomer, and crinine showed better estimated free energy of binding than the reference compound, amphotericin B. In conclusion, this is the first in vitro, in vivo, and in silico report about the antileishmanial potential and alkaloid profiling of the extract of C. milagroanthus bulbs, which could become an interesting source of bioactive molecules |
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2023 |
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2023-10-17T20:38:23Z |
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2023-10-17T20:38:23Z |
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2023-01-10 |
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Artículo de revista |
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Soto-Vásquez, M.R.; Alvarado-García, P.A.A.; Osorio, E.H.; Tallini, L.R.; Bastida, J. Antileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru. Plants 2023, 12, 322. https:// doi.org/10.3390/plants12020322 |
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22237747 |
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Soto-Vásquez, M.R.; Alvarado-García, P.A.A.; Osorio, E.H.; Tallini, L.R.; Bastida, J. Antileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru. Plants 2023, 12, 322. https:// doi.org/10.3390/plants12020322 22237747 |
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eng |
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eng |
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13 |
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322 |
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1 |
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12 |
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Plants |
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Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook. Braz. J. Pharm. Sci. 2017, 53, 15063–15069 Roy, M.; Liang, L.; Xiao, X.; Feng, P.; Ye, M.; Liu, J. Lycorine: A prospective natural lead for anticancer drug discovery. Biomed. Pharmacother. 2018, 107, 615–624 Nair, J.J.; van Staden, J. Antiprotozoal alkaloid principles of the plant family Amaryllidaceae. Bioorg. Med. Chem. Lett. 2019, 29, 12664 Osorio, E.D.; Berkov, S.; Brun, R.; Codina, C.; Viladomat, F.; Cabezas, F.; Bastida, J. In vitro antiprotozoal activity of alkaloids from Phaedranassa dubia (Amaryllidaceae). Phytochem. Lett. 2010, 3, 161–163 Tallini, L.R.; de Andrade, J.P.; Kaiser, M.; Viladomat, F.; Nair, J.J.; Zuanazzi, J.A.S.; Bastida, J. Alkaloid constituents of the Amaryllidaceae plant Amaryllis belladonna L. Molecules 2017, 22, 1437 Labraña, L.; Machocho, A.K.; Kricsfalusy, V.; Brun, R.; Codina, C.; Viladomat, F.; Bastida, J. 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Automated docking using a Lamarckinan genetic algorithm and an empirical binding free energy function. J. Comput. Chem. 1999, 19, 1639–1662 Schrödinger Release 2022-3: Maestro; Schrödinger, LLC.: New York, NY, USA, 2021 |
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Soto-Vásquez, Marilú Roxanac7d2fb41-1daf-4d03-880f-df6ce493f06e-1Alvarado-García, Paul Alan Arkin612c3d0b-2069-4e97-a3d4-5e085d831264-1Osorio, Edison H.087e0c0b-d49f-4915-b7fa-272d785c30af-1Tallini, Luciana R.2f4f4ff0-b0e3-4368-a41a-43b019651013-1Bastida, Jaume00ea29d6-11a2-48bd-bc9e-2dd48a2bc06e-12023-10-17T20:38:23Z2023-10-17T20:38:23Z2023-01-10Leishmaniasis is a worldwide infectious parasitic disease caused by different species of protozoa of the genus Leishmania, which are transmitted to animals and humans through the bite of insects of the Psychodidae family. In the present work, the antileishmanial activity of an alkaloid extract of the bulbs of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) was evaluated in vitro, in vivo, and in silico against the parasite Leishmania braziliensis, and the chemical profile of the sample was determined by GC-MS analysis. At concentrations of 1, 10, and 100 µg·mL−1, the alkaloid extract presented inhibition percentages of 8.7%, 23.1%, and 98.8%, respectively, against L. braziliensis with a p < 0.05, and IC50 values of 18.5 ± 0.3 µg·mL−1. Furthermore, at a dose of 1.0 mg·kg−1, a greater decrease in lesion size was observed (90%) for in vivo assays, as well as a decrease in infection (96%), finding no significant differences (p > 0.05) in comparison with amphotericin B (92% and 98%, respectively). Eleven alkaloids were identified in C. milagroanthus bulbs: galanthamine, vittatine/crinine, 8-O-demethylmaritidine, anhydrolycorine, 11,12-dehydroanhydrolycorine, hippamine, lycorine, 2-hydroxyanhydrolycorine, 7-hydroxyclivonine, 2α-hydroxyhomolycorine, and 7-hydroxyclivonine isomer. A molecular model of Leishmania braziliensis trypanothione reductase (TRLb) was built using computational experiments to evaluate in silico the potential of the Amaryllidaceae alkaloid identified in C. milagroanthus toward this enzyme. The structures galanthamine, 7-hydroxyclivonine isomer, and crinine showed better estimated free energy of binding than the reference compound, amphotericin B. In conclusion, this is the first in vitro, in vivo, and in silico report about the antileishmanial potential and alkaloid profiling of the extract of C. milagroanthus bulbs, which could become an interesting source of bioactive moleculesapplication/pdfSoto-Vásquez, M.R.; Alvarado-García, P.A.A.; Osorio, E.H.; Tallini, L.R.; Bastida, J. Antileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru. 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Leiva & Meerow (Amaryllidaceae) collected in PeruArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublicationTEXTAntileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru - plants-12-00322.pdf.txtAntileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru - plants-12-00322.pdf.txtExtracted texttext/plain4452https://repositorio.unibague.edu.co/bitstreams/d7d64be2-c335-4971-9ad6-226d88073d23/download328d59031b13114085bbb87a91563810MD53THUMBNAILAntileishmanial Activity of Clinanthus milagroanthus S. Leiva & Meerow (Amaryllidaceae) Collected in Peru - plants-12-00322.pdf.jpgAntileishmanial Activity of Clinanthus milagroanthus S. 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