Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas
Objectives: Colombia is a dengue hyperendemic country; however, the prevalence of antibodies against dengue in the general population including the inhabitants of rural areas is unknown. This study aimed to determine the prevalence of dengue IgM and IgG antibodies in healthy children and adults in u...
- Autores:
-
Velandia-Romero, Myriam L.
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/918
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/918
- Palabra clave:
- Seroprevalence Dengue symptomatic Dengue asymptomatic Colombia
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| title |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| spellingShingle |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas Seroprevalence Dengue symptomatic Dengue asymptomatic Colombia |
| title_short |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| title_full |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| title_fullStr |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| title_full_unstemmed |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| title_sort |
Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas |
| dc.creator.fl_str_mv |
Velandia-Romero, Myriam L. |
| dc.contributor.author.none.fl_str_mv |
Velandia-Romero, Myriam L. |
| dc.contributor.other.none.fl_str_mv |
Coronel-Ruiz, Carolina Castro-Bonilla, Lorena Camacho-Ortega, Sigrid Calderón-Peláez, María Angélica Castellanos, Angélica Olano, Víctor Alberto Porras, Alexandra Arevalo-Herrera, Myriam Villamil-Gómez, Wilmer Castellanos, Jaime E. |
| dc.subject.keywords.spa.fl_str_mv |
Seroprevalence Dengue symptomatic Dengue asymptomatic Colombia |
| topic |
Seroprevalence Dengue symptomatic Dengue asymptomatic Colombia |
| description |
Objectives: Colombia is a dengue hyperendemic country; however, the prevalence of antibodies against dengue in the general population including the inhabitants of rural areas is unknown. This study aimed to determine the prevalence of dengue IgM and IgG antibodies in healthy children and adults in urban and rural areas of seven different endemic regions in Colombia between 2013 and 2015. Design or method: Blood samples from healthy volunteers (1,318) were processed by serology (by indirect IgG and capture IgM and IgG ELISA) and molecular tests to detect viral RNA and circulating serotypes. Results: The seroprevalence of IgG for dengue were 85% in children and over 90% for adults. In addition to the high IgM positive rate (14.9%) and secondary recent infection marker rate (capture IgG, 16%), 8.4% of the healthy volunteers were positive for dengue virus (DENV) RNA. Conclusion: This study confirmed the broad and permanent circulation of DENV in Colombia and the high rates of infection and reinfection suffered by its inhabitants. This information can be used by the health authorities to strengthen vector control and vaccine policies and review the algorithms of diagnosis and disease management in children and adults. |
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2019 |
| dc.date.issued.none.fl_str_mv |
2019-05-23 |
| dc.date.submitted.none.fl_str_mv |
2019-10-31 |
| dc.date.accessioned.none.fl_str_mv |
2022-11-15T20:56:57Z |
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2022-11-15T20:56:57Z |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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Artículo |
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publishedVersion |
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https://hdl.handle.net/20.500.12834/918 |
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10.1016/j.ijid.2019.10.045 |
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Universidad del Atlántico |
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Repositorio Universidad del Atlántico |
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https://hdl.handle.net/20.500.12834/918 |
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10.1016/j.ijid.2019.10.045 Universidad del Atlántico Repositorio Universidad del Atlántico |
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eng |
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eng |
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Velandia-Romero, Myriam L.011ba06b-5694-4025-aae3-9d72300aa709Coronel-Ruiz, CarolinaCastro-Bonilla, LorenaCamacho-Ortega, SigridCalderón-Peláez, María AngélicaCastellanos, AngélicaOlano, Víctor AlbertoPorras, AlexandraArevalo-Herrera, MyriamVillamil-Gómez, WilmerCastellanos, Jaime E.2022-11-15T20:56:57Z2022-11-15T20:56:57Z2019-05-232019-10-31https://hdl.handle.net/20.500.12834/91810.1016/j.ijid.2019.10.045Universidad del AtlánticoRepositorio Universidad del AtlánticoObjectives: Colombia is a dengue hyperendemic country; however, the prevalence of antibodies against dengue in the general population including the inhabitants of rural areas is unknown. This study aimed to determine the prevalence of dengue IgM and IgG antibodies in healthy children and adults in urban and rural areas of seven different endemic regions in Colombia between 2013 and 2015. Design or method: Blood samples from healthy volunteers (1,318) were processed by serology (by indirect IgG and capture IgM and IgG ELISA) and molecular tests to detect viral RNA and circulating serotypes. Results: The seroprevalence of IgG for dengue were 85% in children and over 90% for adults. In addition to the high IgM positive rate (14.9%) and secondary recent infection marker rate (capture IgG, 16%), 8.4% of the healthy volunteers were positive for dengue virus (DENV) RNA. Conclusion: This study confirmed the broad and permanent circulation of DENV in Colombia and the high rates of infection and reinfection suffered by its inhabitants. This information can be used by the health authorities to strengthen vector control and vaccine policies and review the algorithms of diagnosis and disease management in children and adults.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areasSeroprevalence Dengue symptomatic Dengue asymptomatic Colombiainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede NorteCalvo E, Sánchez F, Duran S, Castellanos J. Easy and inexpensive molecular detection of dengue, chikungunya and zika viruses in febrile patients. Acta Trop 2016;163:32–7, doi:http://dx.doi.org/10.1016/j.actatropica.2016.07.021.Carabali M, Kyungah J, Vélez D, Trujillo A, Egurrola J, Sung K, et al. Dengue virus serological prevalence and seroconversión rates in children and adults in Medellin, Colombia: implications for vaccine introduction. Int J Infect Dis 2017;58:27–36, doi:http://dx.doi.org/10.1016/j.ijid.2017.02.016.Castellanos J, Coronel C, Parra S, Castilla M, Calvo E, Arévalo M, et al. Description of high rates of inapparent and simultaneous multiple dengue virus infection in a Colombian jungle. Trop Biomed 2016;33:375–82.Castro L, Coronel-Ruiz C, Castellanos J, Porras A, Parra-Álvarez S, Velandia-Romero ML. Factors associated with dengue virus infection and reinfection in asymptomatic M.L. Velandia-Romero et al. / International Journal of Infectious Diseases 91 (2020) 9–16 15 children in two Colombianmunicipalities. AmJ Trop Med Hyg 2018;99(6):1422–9, doi:http://dx.doi.org/10.4269/ajtmh.17-0617.Castro Rodríguez R, Galera-Gelvez K, López Yescas J, Rueda-Gallardo J. Costs of dengue to the health system and individuals in Colombia from 2010 to 2012. Am J Trop Med Hyg 2015;92(4):709–14, doi:http://dx.doi.org/10.4269/ ajtmh.14-0386.Chien L, Liao T, Shu P, Huang J, Gubler D, Chang G. Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses. J Clin Microbiol 2006;44:1295–304, doi:http://dx.doi.org/10.1128/JCM.44.4.1295-1304.2006.Chien YW, Liu ZH, Tseng FC, Ho TC, Guo HR, Ko NY, Ko WC, et al. Prolonged persistence of IgM against dengue virus detected by commonly used commercial assays. BMC Infect Dis 2018;18:156, doi:http://dx.doi.org/ 10.1186/s12879-018-3058-0.Chien YW, Huang HM, Ho TC, Tseng FC, Ko NY, Ko WC, et al. Seroepidemiology of dengue virus infection among adults during the ending phase of a severe dengue epidemic in southern Taiwan, 2015. BMC Infect Dis 2019;19:338, doi: http://dx.doi.org/10.1186/s12879-019-3946-y.Dayan G, Arredondo J, Carrasquilla G, Deseda C, Dietze R, Luz K, et al. Prospective cohort study with active surveillance for fever in four dengue endemic countries in Latin America. Am J Trop Med Hyg 2015;93:18–23, doi:http://dx. doi.org/10.4269/ajtmh.13-0663.Dean AG, Sullivan KM, Soe MM. OpenEpi: Open Source Epidemiologic Statistics for Public Health, Version. 2013 Avalaible at: www.OpenEpi.com. [Updated 6 April 2013]. [Accessed 26 May 2018].Development plan of Sincelejo 2016–2019. Available at: http://www.alcaldiade- sincelejo.gov.co/Ciudadanos/ProyectosNormatividad/Plan%20de%20Desarrollo %20Municipal%202016%20-%202019.pdf. [Accessed 13 April 2018]. [Spanish].Díaz-Quijano FA, Martínez-Vega RA, Rodríguez-Morales AJ, Rojas-Calero RA, Luna- González ML, Díaz-Quijano RG. Association between the level of education and knowledge, attitudes and practices regarding dengue in the Caribbean region of Colombia. BMC Public Health 2018;18(1):143, doi:http://dx.doi.org/10.1186/ s12889-018-5055-z.Forero D, Chaparro P, Vallejo A, Benavides Y, Gutiérrez J, Arévalo-Herrera M, et al. Knowledge, attitudes and practices of malaria in Colombia. Malar J 2014;13:165, doi:http://dx.doi.org/10.1186/1475-2875-13-165.Governorship of Valle del Cauca. Available at: http://www.valledelcauca.gov.co/ publicaciones.php?id=32. [Accessed 13 April 2018]. [Spanish].Guzmán M, Kouri G. Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges. J Clin Virol 2003;27:1–13.Hadinegoro SR, Arredondo García J, Capeding M, Deseda C, Chotpitayasunondh T, Dietze R, et al. Efficacy and long-term safety of a dengue vaccine in regions of endemic disease. N Engl J Med 2015;373:1195–206, doi:http://dx.doi.org/ 10.1056/NEJMoa1506223.Halstead S. Licensed dengue vaccine: public health conundrum and scientific challenge. Am J Trop Med Hyg 2016;95:741–5, doi:http://dx.doi.org/10.4269/ ajtmh.16-0222.Halstead S. Dengue in the Americas and Southeast Asia: Do they differ?. Rev Panam Salud Pública 2007;20:407–15.Hammon S, Balmaseda A, Pérez L, Téllez Y, Saborio S, Mercado J, et al. Difference in dengue severity in infants, children, and adults in a 3-year hospital-based study in Nicaragua. Am J Trop Med Hyg 2005;73:1063–70.Jiménez M, Arias J, Carrasquilla G. Seroprevalence of dengue infection in the municipalities of Armenia, Calarcá, La Tebaida and Montenegro in Quindío, 2014. Biomédica 2017;37:34–41, doi:http://dx.doi.org/10.7705/biomedica. v37i1.3208 [Spanish].Liu J, Deng Y, Jing Q, Chen X, Du Z, Liang T, et al. Dengue infection spectrum in Guangzhou: a cross-sectional seroepidemiology study among community residents between 2013 and 2015. Int J Environ Res Public Health 2018;15(6), doi:http://dx.doi.org/10.3390/ijerph15061227 pii: E1227.Martins-Melo F, Carneiro M, Ramos Jr A, Heukelbach J, Ribeiro ALP, Werneck G. The burden of Neglected Tropical Diseases in Brazil, 1990-2016: a subnational analysis from the Global Burden of Disease Study 2016. PLoS Negl Trop Dis 2018;12(6)e0006559, doi:http://dx.doi.org/10.1371/journal.pntd.0006559.Muhammad Azami N, Salleh S, Neoh H, Syed S, Jamal R. Dengue epidemic in Malaysia: not a predominantly urban disease anymore. BMC Res Notes 2011;4:216, doi:http://dx.doi.org/10.1186/1756-0500-4-216.National Institute of Health. Dengue final report, Colombia. 2010 Available at: https://www.ins.gov.co/buscadoreventos/Informesdeevento/Dengue%202010. pdf. [Accessed 29 May 2018]. [Spanish].National Institute of Health. Dengue final report, Colombia. 2012 Available at: https://www.ins.gov.co/buscadoreventos/Informesdeevento/Dengue%202012. pdf. [Accessed 29 May 2018]. [Spanish].National Institute of Health. Dengue final report, Colombia. 2013 Available at: http://www.ins.gov.co/lineasdeaccion/SubdireccionVigilancia/Informe%20de% 20Evento%20Epidemiolgico/DENGUE%202013.pdf. [Accessed 4 May 2018]. [Spanish].National Institute of Health. Dengue final report, Colombia. 2014 Available at: http://www.ins.gov.co/lineasdeaccion/SubdireccionVigilancia/Informe%20de% 20Evento%20Epidemiolgico/Dengue%202014.pdf. [Accessed 29 May 29 2018]. [Spanish].National Institute of Health. Dengue final report, Colombia. 2015 Available at: http://www.ins.gov.co/buscadoreventos/Informesdeevento/Dengue%202014. pdf. [Accessed 29 May 2018]. [Spanish].Olano VA, Matiz M, Lenhart A, Vargas S, Jaramillo J, Sarmiento D, et al. Schools as potential risk sites for vector-borne disease transmission: mosquito vectors in rural schools in two municipalities in Colombia. J Am Mosq Control Assoc 2015;31(3):212–22, doi:http://dx.doi.org/10.2987/moco-31-03-212-222.1.Padilla J, Rojas D, Sáenz-Gómez R. Dengue in Colombia: Epidemiology of Hyperendemic Re-emergence] Guías de impresión. (Bogotá, Colombia) ISBN: 9789584606617. [Spanish]. 2012.Pérez R, Castellanos J, Olano V, Matiz M, Jaramillo J, Vargas S, et al. Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia. Mem Inst Oswaldo Cruz 2016;111:233–40, doi:http://dx.doi. org/10.1590/0074-02760150363.Quibdó town hall. Available at: https://www.quibdochoco.gov.co/Institucional/ Paginas/Informacion-del-Municipio.aspx. [Accessed: 13 April 2018]. [Spanish].Ruiz F, González A, Vélez A, Gómez G. Presence of Aedes (Stegomyia) aegypti (Linnaeus, 1762) and its natural infection with dengue virus at unrecorded heights in Colombia. Biomédica 2016;36:303–8, doi:http://dx.doi.org/10.7705/ biomedica. v36i2.3301Sacramento R, de Carvalho Araújo F, Lima D, Alencar C, Martins V, Araújo LV, et al. Dengue fever and Aedes aegypti in Indigenous Brazilians: seroprevalence, risk factors, knowledge and practices. Trop Med Int Health 2018;23(6):596–604, doi:http://dx.doi.org/10.1111/tmi.13061Secretariat of Planning of Cundinamarca Office of Information Systems Analysis and Statistics. Basic Statistics Province of Tequendama. Available at: http://www. cundinamarca.gov.co/wps/wcm/connect/b0515f7a-9fb9-481a-955e- edb962c9c50d/Tequendama.pdf?MOD=AJPERES. [Accessed 14 April 2017]. [Spanish].Sincelejo town hall. Available at: http://www.alcaldiadesincelejo.gov.co/MiMu- nicipio/Paginas/InformaciondelMunicipio.aspx. [Accessed: 13 April 2018]. [Spanish].Teixeira T, Costa MdaC, Barreto F, Barreto ML. Dengue: twenty-five years since reemergence in Brazil. Cad Saude Publica 2009;25 supp1:S1–S18, doi:http://dx. doi.org/10.1590/S0102-311X2009001300002.Tuesca-Molina R, Navarro-Lechuga E, Goenaga-Jiménez EC, Martínez-Garcés, Acosta-Reyes J. Sero-prevalence in a hyperendemic area of dengue: Barranquilla- Colombia. Aquichan 2018;18(1)95–109, doi:http://dx.doi.org/10.5294/ aqui.2018.18.1.9 [Spanish].Tumaco town hall. Available at: http://www.tumaconarino.gov.co/municipio/ nuestro-municipio. [Accessed 13 April 2018]. [Spanish].Villar L, Rojas D, Besada-Lombana S, Sarti E. Epidemiological trends of dengue disease in Colombia (2000-2011): a systematic review. PLoS Negl Trop Dis 2015a;9(3)e0003499, doi:http://dx.doi.org/10.1371/journal.pntd.0003499.Villar L, Dayan G, Arredondo-García J, Rivera D, Cunha R, Deseda C, et al. Efficacy of a tetravalent dengue vaccine in children in Latin America. N Engl J Med 2015b;372:113–23, doi:http://dx.doi.org/10.1056/NEJMoa1411037Waggoner JJ, Gresh L, Vargas MJ, Ballesteros G, Tellez Y, Soda KJ, et al. Viremia and clinical presentation in Nicaraguan patients infected with zika virus, chikungunya virus, and dengue virus. Clin Infect Dis 2016;63(December (12)):1584–90, doi:http://dx.doi.org/10.1093/cid/ciw589.World Health Organization and Pan American Health Organization. Dengue cases in the Americas. . p. 1–3 Available from: http://www.who.int/mediacentre/ factsheets/2017-cha-dengue-cases-mar-27-ew-11.pdf?ua=1. [Accessed 17 May 17 2018].Yap G, Li C, Mutalib A, Lai YL, Ng LC. High rates of inapparent dengue in older adults in Singapore. 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