Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications
Recently, tissue engineering became a very important medical alternative in patients who need to regenerate damaged or lost tissues through the use of scaffolds that support cell adhesion and proliferation. Carbon nanomaterials (carbon nanotubes, fullerenes, multi-wall fullerenes, and graphene) beca...
- Autores:
-
Grande Tovar, Carlos David
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/1164
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/1164
- Palabra clave:
- biodegradable films
chitosan
oxidized carbon nano-onions
poly(vinyl alcohol)
tissue engineering
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| title |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| spellingShingle |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications biodegradable films chitosan oxidized carbon nano-onions poly(vinyl alcohol) tissue engineering |
| title_short |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| title_full |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| title_fullStr |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| title_full_unstemmed |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| title_sort |
Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications |
| dc.creator.fl_str_mv |
Grande Tovar, Carlos David |
| dc.contributor.author.none.fl_str_mv |
Grande Tovar, Carlos David |
| dc.contributor.other.none.fl_str_mv |
Castro, Jorge Iván Valencia, Carlos Humberto Navia Porras, Diana Paola Mina Hernandez, José Herminsul Valencia, Mayra Eliana Velásquez, José Daniel Chaur, Manuel N. |
| dc.subject.keywords.spa.fl_str_mv |
biodegradable films chitosan oxidized carbon nano-onions poly(vinyl alcohol) tissue engineering |
| topic |
biodegradable films chitosan oxidized carbon nano-onions poly(vinyl alcohol) tissue engineering |
| description |
Recently, tissue engineering became a very important medical alternative in patients who need to regenerate damaged or lost tissues through the use of scaffolds that support cell adhesion and proliferation. Carbon nanomaterials (carbon nanotubes, fullerenes, multi-wall fullerenes, and graphene) became a very important alternative to reinforce the mechanical, thermal, and antimicrobial properties of several biopolymers. In this work, five different formulations of chitosan/poly(vinyl alcohol)/oxidized carbon nano-onions (CS/PVA/ox-CNO) were used to prepare biodegradable scaffolds with potential biomedical applications. Film characterization consisted of Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), tension strength, Young’s modulus, X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). The degradation in a simulated body fluid (FBS) demonstrated that all the formulations lost between 75% and 80% of their weight after 15 days of treatment, but the degradation decreased with the ox-CNO content. In vivo tests after 90 days of subdermal implantation of the nanocomposite films in Wistar rats’ tissue demonstrated good biocompatibility without allergenic reactions or pus formation. There was a good correlation between FBS hydrolytic degradation and degradation in vivo for all the samples, since the ox-CNO content increased the stability of the material. All these results indicate the potential of the CS/PVA/ox-CNO nanocomposite films in tissue engineering, especially for long-term applications. |
| publishDate |
2019 |
| dc.date.issued.none.fl_str_mv |
2019-11-01 |
| dc.date.submitted.none.fl_str_mv |
2019-10-08 |
| dc.date.accessioned.none.fl_str_mv |
2023-01-17T16:17:06Z |
| dc.date.available.none.fl_str_mv |
2023-01-17T16:17:06Z |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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Artículo |
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publishedVersion |
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https://hdl.handle.net/20.500.12834/1164 |
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10.3390/biom9110684 |
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Universidad del Atlántico |
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Repositorio Universidad del Atlántico |
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https://hdl.handle.net/20.500.12834/1164 |
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10.3390/biom9110684 Universidad del Atlántico Repositorio Universidad del Atlántico |
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eng |
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Grande Tovar, Carlos David9e800b3a-a886-44a8-9872-71464aa6e429Castro, Jorge IvánValencia, Carlos HumbertoNavia Porras, Diana PaolaMina Hernandez, José HerminsulValencia, Mayra ElianaVelásquez, José DanielChaur, Manuel N.2023-01-17T16:17:06Z2023-01-17T16:17:06Z2019-11-012019-10-08https://hdl.handle.net/20.500.12834/116410.3390/biom9110684Universidad del AtlánticoRepositorio Universidad del AtlánticoRecently, tissue engineering became a very important medical alternative in patients who need to regenerate damaged or lost tissues through the use of scaffolds that support cell adhesion and proliferation. Carbon nanomaterials (carbon nanotubes, fullerenes, multi-wall fullerenes, and graphene) became a very important alternative to reinforce the mechanical, thermal, and antimicrobial properties of several biopolymers. In this work, five different formulations of chitosan/poly(vinyl alcohol)/oxidized carbon nano-onions (CS/PVA/ox-CNO) were used to prepare biodegradable scaffolds with potential biomedical applications. Film characterization consisted of Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), tension strength, Young’s modulus, X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). The degradation in a simulated body fluid (FBS) demonstrated that all the formulations lost between 75% and 80% of their weight after 15 days of treatment, but the degradation decreased with the ox-CNO content. In vivo tests after 90 days of subdermal implantation of the nanocomposite films in Wistar rats’ tissue demonstrated good biocompatibility without allergenic reactions or pus formation. There was a good correlation between FBS hydrolytic degradation and degradation in vivo for all the samples, since the ox-CNO content increased the stability of the material. All these results indicate the potential of the CS/PVA/ox-CNO nanocomposite films in tissue engineering, especially for long-term applications.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Preparation of chitosan/poly(Vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applicationsPúblico generalbiodegradable filmschitosanoxidized carbon nano-onionspoly(vinyl alcohol)tissue engineeringinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede Norte1. Kelleher, C.M.; Vacanti, J.P. Engineering extracellular matrix through nanotechnology. J. R. Soc. Interface 2010, 7, S717–S7292. Guo, B.; Sun, Y.; Finne-Wistrand, A.; Mustafa, K.; Albertsson, A.-C. Electroactive porous tubular scaffolds with degradability and non-cytotoxicity for neural tissue regeneration. Acta Biomater. 2012, 8, 144–153.3. Agarwal, S.; Wendorff, J.H.; Greiner, A. Use of electrospinning technique for biomedical applications. Polymer 2008, 49, 5603–56214. Gomes, M.E.; Azevedo, H.S.; Moreira, A.R.; Ellä, V.; Kellomäki, M.; Reis, R.L. Starch–poly (ε-caprolactone) and starch–poly (lactic acid) fibre-mesh scaffolds for bone tissue engineering applications: Structure, mechanical properties and degradation behaviour. J. Tissue Eng. Regen. Med. 2008, 2, 243–2525. Hollister, S.J. Porous scaffold design for tissue engineering. Nat. Mater. 2005, 4, 518–5246. Antunes, J.C.; Oliveira, J.M.; Reis, R.L.; Soria, J.M.; Gómez-Ribelles, J.L.; Mano, J.F. 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Biomaterials 2018, 164, 22–37http://purl.org/coar/resource_type/c_6501ORIGINALPreparationofChitosanPolyVinylAlcoholNanocompositeFilmsIncorporatedwithOxidized.pdfPreparationofChitosanPolyVinylAlcoholNanocompositeFilmsIncorporatedwithOxidized.pdfapplication/pdf9319187https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1164/1/PreparationofChitosanPolyVinylAlcoholNanocompositeFilmsIncorporatedwithOxidized.pdf20fc7c01ad8671f7a4715af20d1deb16MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1164/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1164/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/1164oai:repositorio.uniatlantico.edu.co:20.500.12834/11642023-01-17 11:17:07.45DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.coVMOpcm1pbm9zIGdlbmVyYWxlcyBkZWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbCBkZSBsYSBVbml2ZXJzaWRhZCBkZWwgQXRsw6FudGljbwoKRWwgKGxvcykgYXV0b3IgKGVzKSBoYW4gYXNlZ3VyYWRvIChuKSBsbyBzaWd1aWVudGUgc29icmUgbGEgb2JyYSBhIGludGVncmFyIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwsIHF1ZToKCuKXjwlFcyBvcmlnaW5hbCwgZGUgc3UgZXhjbHVzaXZhIGF1dG9yw61hLCBzZSByZWFsaXrDsyBzaW4gdmlvbGFyIG8gdXN1cnBhciBkZXJlY2hvcyBkZSBhdXRvciBkZSB0ZXJjZXJvcyB5IHBvc2VlIGxhIHRpdHVsYXJpZGFkLgril48JQXN1bWlyw6FuIGxhIHJlc3BvbnNhYmlsaWRhZCB0b3RhbCBwb3IgZWwgY29udGVuaWRvIGEgbGEgb2JyYSBhbnRlIGxhIEluc3RpdHVjacOzbiB5IHRlcmNlcm9zLgril48JQXV0b3JpemFuIGEgdMOtdHVsbyBncmF0dWl0byB5IHJlbnVuY2lhcyBhIHJlY2liaXIgZW1vbHVtZW50b3MgcG9yIGxhcyBhY3RpdmlkYWRlcyBxdWUgc2UgcmVhbGljZW4gY29uIGVsbGEsIHNlZ8O6biBzdSBsaWNlbmNpYS4KCgpMYSBVbml2ZXJzaWRhZCBkZWwgQXRsw6FudGljbywgcG9yIHN1IHBhcnRlLCBzZSBjb21wcm9tZXRlIGEgYWN0dWFyIGVuIGxvcyB0w6lybWlub3MgZXN0YWJsZWNpZG9zIGVuIGxhIExleSAyMyBkZSAxOTgyIHkgbGEgRGVjaXNpw7NuIEFuZGluYSAzNTEgZGUgMTk5MywgZGVtw6FzIG5vcm1hcyBnZW5lcmFsZXMgc29icmUgbGEgbWF0ZXJpYSB5IGVsIEFjdWVyZG8gU3VwZXJpb3IgMDAxIGRlIDE3IGRlIG1hcnpvIGRlIDIwMTEsIHBvciBtZWRpbyBkZWwgY3VhbCBzZSBleHBpZGUgZWwgRXN0YXR1dG8gZGUgUHJvcGllZGFkIEludGVsZWN0dWFsIGRlIGxhIFVuaXZlcnNpZGFkIGRlbCBBdGzDoW50aWNvLgoKUG9yIMO6bHRpbW8sIGhhbiBzaWRvIGluZm9ybWFkb3Mgc29icmUgZWwgdHJhdGFtaWVudG8gZGUgZGF0b3MgcGVyc29uYWxlcyBwYXJhIGZpbmVzIGFjYWTDqW1pY29zIHkgZW4gYXBsaWNhY2nDs24gZGUgY29udmVuaW9zIGNvbiB0ZXJjZXJvcyBvIHNlcnZpY2lvcyBjb25leG9zIGNvbiBhY3RpdmlkYWRlcyBwcm9waWFzIGRlIGxhIGFjYWRlbWlhLCBiYWpvIGVsIGVzdHJpY3RvIGN1bXBsaW1pZW50byBkZSBsb3MgcHJpbmNpcGlvcyBkZSBsZXkuCgpMYXMgY29uc3VsdGFzLCBjb3JyZWNjaW9uZXMgeSBzdXByZXNpb25lcyBkZSBkYXRvcyBwZXJzb25hbGVzIHB1ZWRlbiBwcmVzZW50YXJzZSBhbCBjb3JyZW8gZWxlY3Ryw7NuaWNvIGhhYmVhc2RhdGFAbWFpbC51bmlhdGxhbnRpY28uZWR1LmNvCg== |