In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties

In this study, a family of porphyrins based on 5,10,15,20-Tetrakis(4-ethylphenyl)porphyrin (1, Ph) and six metallo-derivatives (Zn2+(2, Ph-Zn), Sn4+(3, Ph-Sn), Mn2+ (4, Ph-Mn), Ni2+ (5, Ph-Ni), Al3+ (6, Ph-Al), and V3+ (7, Ph-V)) were tested as photosensitizers for photodynamic therapy against Leish...

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Autores:
Espitia-Almeida, Fabian
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Atlántico
Repositorio:
Repositorio Uniatlantico
Idioma:
eng
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oai:repositorio.uniatlantico.edu.co:20.500.12834/965
Acceso en línea:
https://hdl.handle.net/20.500.12834/965
Palabra clave:
porphyrin; metalloporphyrins; photodynamic therapy; Leishmania braziliensis; Leishmania panamensis; singlet oxygen
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openAccess
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http://creativecommons.org/licenses/by-nc/4.0/
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dc.title.spa.fl_str_mv In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
title In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
spellingShingle In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
porphyrin; metalloporphyrins; photodynamic therapy; Leishmania braziliensis; Leishmania panamensis; singlet oxygen
title_short In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
title_full In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
title_fullStr In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
title_full_unstemmed In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
title_sort In vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes properties
dc.creator.fl_str_mv Espitia-Almeida, Fabian
dc.contributor.author.none.fl_str_mv Espitia-Almeida, Fabian
dc.contributor.other.none.fl_str_mv Díaz-Uribe, Carlos
Vallejo, William
Gómez-Camargo, Doris
Romero Bohórquez, Arnold R.
dc.subject.keywords.spa.fl_str_mv porphyrin; metalloporphyrins; photodynamic therapy; Leishmania braziliensis; Leishmania panamensis; singlet oxygen
topic porphyrin; metalloporphyrins; photodynamic therapy; Leishmania braziliensis; Leishmania panamensis; singlet oxygen
description In this study, a family of porphyrins based on 5,10,15,20-Tetrakis(4-ethylphenyl)porphyrin (1, Ph) and six metallo-derivatives (Zn2+(2, Ph-Zn), Sn4+(3, Ph-Sn), Mn2+ (4, Ph-Mn), Ni2+ (5, Ph-Ni), Al3+ (6, Ph-Al), and V3+ (7, Ph-V)) were tested as photosensitizers for photodynamic therapy against Leishmania braziliensis and panamensis. The singlet oxygen quantum yield value (FD) for (1–7) was measured using 1,3-diphenylisobenzofuran (DPBF) as a singlet oxygen trapping agent and 5,10,15,20-(tetraphenyl)-porphyrin (H2TPP) as a reference standard; besides, parasite viability was estimated by the MTT assay. After metal insertion into the porphyrin core, the FD increased from 0.76–0.90 and cell viability changed considerably. The FD and metal type changed the cytotoxic activity. Finally, (2) showed both the highest FD (0.90) and the best photodynamic activity against the parasites studied (IC50 of 1.2 M).
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-04-19
dc.date.submitted.none.fl_str_mv 2020-03-23
dc.date.accessioned.none.fl_str_mv 2022-11-15T21:16:14Z
dc.date.available.none.fl_str_mv 2022-11-15T21:16:14Z
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.hasVersion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.spa.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12834/965
dc.identifier.doi.none.fl_str_mv doi:10.3390/molecules25081887
dc.identifier.instname.spa.fl_str_mv Universidad del Atlántico
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad del Atlántico
url https://hdl.handle.net/20.500.12834/965
identifier_str_mv doi:10.3390/molecules25081887
Universidad del Atlántico
Repositorio Universidad del Atlántico
dc.language.iso.spa.fl_str_mv eng
language eng
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dc.publisher.place.spa.fl_str_mv Barranquilla
dc.publisher.sede.spa.fl_str_mv Sede Norte
dc.source.spa.fl_str_mv MDPI AG
institution Universidad del Atlántico
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spelling Espitia-Almeida, Fabianef3c5f17-3cd2-46d9-b512-479b797bbcfcDíaz-Uribe, CarlosVallejo, WilliamGómez-Camargo, DorisRomero Bohórquez, Arnold R.2022-11-15T21:16:14Z2022-11-15T21:16:14Z2020-04-192020-03-23https://hdl.handle.net/20.500.12834/965doi:10.3390/molecules25081887Universidad del AtlánticoRepositorio Universidad del AtlánticoIn this study, a family of porphyrins based on 5,10,15,20-Tetrakis(4-ethylphenyl)porphyrin (1, Ph) and six metallo-derivatives (Zn2+(2, Ph-Zn), Sn4+(3, Ph-Sn), Mn2+ (4, Ph-Mn), Ni2+ (5, Ph-Ni), Al3+ (6, Ph-Al), and V3+ (7, Ph-V)) were tested as photosensitizers for photodynamic therapy against Leishmania braziliensis and panamensis. The singlet oxygen quantum yield value (FD) for (1–7) was measured using 1,3-diphenylisobenzofuran (DPBF) as a singlet oxygen trapping agent and 5,10,15,20-(tetraphenyl)-porphyrin (H2TPP) as a reference standard; besides, parasite viability was estimated by the MTT assay. After metal insertion into the porphyrin core, the FD increased from 0.76–0.90 and cell viability changed considerably. The FD and metal type changed the cytotoxic activity. Finally, (2) showed both the highest FD (0.90) and the best photodynamic activity against the parasites studied (IC50 of 1.2 M).application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2MDPI AGIn vitro anti-leishmanial effect of metallic meso-substituted porphyrin derivatives against Leishmania braziliensis and Leishmania panamensis promastigotes propertiesPúblico generalporphyrin; metalloporphyrins; photodynamic therapy; Leishmania braziliensis; Leishmania panamensis; singlet oxygeninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede Norte1. WHO Leishmaniasis. Available online: https://www.who.int/news-room/fact-sheets/detail/leishmaniasis (accessed on 12 February 2019).2. Alvar, J.; Vélez, I.D.; Bern, C.; Herrero, M.; Desjeux, P.; Cano, J.; Jannin, J.; den Boer, M.; WHO Leishmaniasis Control Team. LeishmaniasisWorldwide and Global Estimates of Its Incidence. PLoS ONE 2012, 7, e356713. Khademvatan, S.; Salmanzadeh, S.; Foroutan-Rad, M.; Bigdeli, S.; Hedayati-Rad, F.; Saki, J.; Heydari-Gorji, E. Spatial distribution and epidemiological features of cutaneous leishmaniasis in southwest of Iran. Alex. J. Med. 2017, 53, 93–98.4. Steverding, D. The history of leishmaniasis. Parasit. Vectors 2017, 10, 82.5. Reithinger, R.; Dujardin, J.-C.; Louzir, H.; Pirmez, C.; Alexander, B.; Brooker, S. Cutaneous leishmaniasis. Lancet Infect. Dis. 2007, 7, 581–596.6. Desjeux, P. Leishmaniasis: Current situation and new perspectives. Comp. Immunol. Microbiol. Infect. Dis. 2004, 27, 305–318.7. Patiño-Londoño, S.Y.; Salazar, L.M.; Acero, C.T.; Bernal, I.D.V. Aspectos socioepidemiológicos y culturales de la leishmaniasis cutánea concepciones, actitudes y prácticas en las poblaciones de Tierralta y Valencia, (Córdoba, Colombia). Salud Colect. 2017, 13, 123–138.8. Gore Saravia, N.; Nicholls, R.S. Leishmaniasis: Un reto para la salud pública que exige concertación de voluntades y esfuerzos. Biomédica 2012, 26, 5.9. Singh, S.; Sivakumar, R. Challenges and new discoveries in the treatment of leishmaniasis. J. Infect. Chemother. 2004, 10, 307–315.10. Lockwood, D.; Moore, E. Treatment of visceral leishmaniasis. J. Glob. Infect. Dis. 2010, 2, 151.11. Tuon, F.F.; Amato, V.S.; Graf, M.E.; Siqueira, A.M.; Nicodemo, A.C.; Neto, V.A. Treatment of New World cutaneous leishmaniasis - a systematic review with a meta-analysis. Int. J. Dermatol. 2008, 47, 109–124.12. Monge-Maillo, B.; López-Vélez, R. Therapeutic Options for OldWorld Cutaneous Leishmaniasis and New World Cutaneous and Mucocutaneous Leishmaniasis. Drugs 2013, 73, 1889–1920.13. Araujo-Melo, M.H.; Meneses, A.M.; Schubach, A.O.; Moreira, J.S.; Conceição-Silva, F.; Salgueiro, M.M.; Pimentel, M.I.F.; Araújo-Silva, M.; Oliveira, R.V.C.; Carmo, C.N.; et al. Risk factors associated with dizziness during treatment of mucosal leishmaniasis with meglumine antimoniate: 16-year retrospective study of cases from Rio de Janeiro, Brazil. J. Laryngol. Otol. 2010, 124, 1056–1060.14. Oliveira, L.F.; Schubach, A.O.; Martins, M.M.; Passos, S.L.; Oliveira, R.V.; Marzochi, M.C.; Andrade, C.A. Systematic review of the adverse e ects of cutaneous leishmaniasis treatment in the New World. Acta Trop. 2011, 118, 87–96.15. Berman, J.D. Chemotherapy for leishmaniasis: Biochemical mechanisms, clinical e cacy, and future strategies. Rev. Infect. Dis. 1988, 10, 560–586.16. Heruti, R.J.; Sharabi, Y.; Arbel, Y.; Shochat, T.; Swartzon, M.; Brenner, G.; Justo, D. ORIGINAL RESEARCH—EPIDEMIOLOGY: The Prevalence of Erectile Dysfunction Among Hypertensive and Prehypertensive Men Aged 25–40 Years. J. Sex. Med. 2007, 4, 596–601.17. Olliaro, P.L.; Guerin, P.J.; Gerstl, S.; Haaskjold, A.A.; Rottingen, J.-A.; Sundar, S. Treatment options for visceral leishmaniasis: A systematic review of clinical studies done in India, 1980–2004. Lancet Infect. Dis. 2005, 5, 763–774.18. Renslo, A.R.; McKerrow, J.H. Drug discovery and development for neglected parasitic diseases. Nat. Chem. Biol. 2006, 2, 701–710.19. Al-Qahtani, A.; Alkahtani, S.; Kolli, B.; Tripathi, P.; Dutta, S.; Al-Kahtane, A.A.; Jiang, X.J.; Ng, D.K.P.; Chang, K.P. Aminophthalocyanine-mediated photodynamic inactivation of Leishmania tropica. Antimicrob. Agents Chemother. 2016, 60, 2003–2011.20. Peloi, L.S.; Biondo, C.E.G.; Kimura, E.; Politi, M.J.; Lonardoni, M.V.C.; Aristides, S.M.A.; Dorea, R.C.C.; Hioka, N.; Silveira, T.G.V. Photodynamic therapy for American cutaneous leishmaniasis: The e cacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis. Exp. Parasitol. 2011, 128, 353–356.21. Jeong, H.-G.; Choi, M.-S. Design and Properties of Porphyrin-based Singlet Oxygen Generator. Isr. J. Chem. 2016, 56, 110–118.22. Josefsen, L.B.; Boyle, R.W. Photodynamic Therapy and the Development of Metal-Based Photosensitisers. Met. Based Drugs 2008, 2008.23. Skwor, T.A.; Klemm, S.; Zhang, H.; Schardt, B.; Blaszczyk, S.; Bork, M.A. Photodynamic inactivation of methicillin-resistant Staphylococcus aureus and Escherichia coli: A metalloporphyrin comparison. J. Photochem. Photobiol. B Biol. 2016, 165, 51–57.24. Mathai, S.; Smith, T.A.; Ghiggino, K.P. Singlet oxygen quantum yields of potential porphyrin-based photosensitisers for photodynamic therapy. Photochem. Photobiol. Sci. 2007, 6, 995–1002.25. Ormond, A.B.; Freeman, H.S. E ects of substituents on the photophysical properties of symmetrical porphyrins. Dye Pigment. 2013, 96, 440–448.26. Cauzzo, G.; Gennari, C.; Jori, G.; Spikes, J.D. The e ect of chemical structure on the photosensitizing e ciencies of porphyrins. Photochem. Photobiol. 1977, 25, 389–395.27. Lavi, A.; Weitman, H.; Holmes, R.T.; Smith, K.M.; Ehrenberg, B. The depth of porphyrin in a membrane and the membrane’s physical properties a ect the photosensitizing e ciency. Biophys. J. 2002, 82, 2101–2110.28. Lesar, A.; Begi´c, G.; Malatesti, N.; Gobin, I. Innovative approach in Legionella water treatment with photodynamic cationic amphiphilic porphyrin. Water Sci. Technol. Water Supply 2019, 19, 1473–1479.29. Rojkiewicz, M.; Ku´s, P.; Kozub, P.; Kempa, M. The synthesis of new potential photosensitizers. Dye Pigment. 2013, 99, 627–635.30. Thomas, M.; Craik, J.D.; Tovmasyan, A.; Batinic-Haberle, I.; Benov, L.T. Amphiphilic cationic Zn-porphyrins with high photodynamic antimicrobial activity. Future Microbiol. 2015, 10, 709–724.31. Ezzeddine, R.; Al-Banaw, A.; Tovmasyan, A.; Craik, J.D.; Batinic-Haberle, I.; Benov, L.T. E ect of molecular characteristics on cellular uptake, subcellular localization, and phototoxicity of Zn(2) N-Alkylpyridylporphyrins. J. Biol. Chem. 2013, 288, 36579–36588.32. Hosomizu, K.; Oodoi, M.; Umeyama, T.; Matano, Y.; Yoshida, K.; Isoda, S.; Isosomppi, M.; Tkachenko, N.V.; Lemmetyinen, H.; Imahori, H. Substituent e ects of porphyrins on structures and photophysical properties of amphiphilic porphyrin aggregates. J. Phys. Chem. B 2008, 112, 16517–16524.33. Stasheuski, A.S.; Galievsky, V.A.; Knyukshto, V.N.; Ghazaryan, R.K.; Gyulkhandanyan, A.G.; Gyulkhandanyan, G.V.; Dzhagarov, B.M.Water-Soluble Pyridyl Porphyrins with Amphiphilic N-Substituents: Fluorescent Properties and Photosensitized Formation of Singlet Oxygen. J. Appl. Spectrosc. 2014, 80, 813–823.34. Gardner, D.M.; Taylor, V.M.; Cedeño, D.L.; Padhee, S.; Robledo, S.M.; Jones, M.A.; Lash, T.D.; Vélez, I.D. Association of Acenaphthoporphyrins with Liposomes for the Photodynamic Treatment of Leishmaniasis. Photochem. Photobiol. 2010, 86, 645–652.35. Pinto, J.G.; Pereira, A.H.C.; de Oliveira, M.A.; Kurachi, C.; Raniero, L.J.; Ferreira-Strixino, J. Chlorin E6 phototoxicity in L. major and L. braziliensis promastigotes—In vitro study. Photodiagn. Photodyn. Ther. 2016, 15, 19–24.36. Bristow, C.-A.; Hudson, R.; Paget, T.A.; Boyle, R.W. Potential of cationic porphyrins for photodynamic treatment of cutaneous Leishmaniasis. Photodiagn. Photodyn. Ther. 2006, 3, 162–167.37. Gomes, M.L.; DeFreitas-Silva, G.; dos Reis, P.G.; Melo, M.N.; Frézard, F.; Demicheli, C.; Idemori, Y.M. Synthesis and characterization of bismuth(III) and antimony(V) porphyrins: High antileishmanial activity against antimony-resistant parasite. JBIC J. Biol. Inorg. Chem. 2015, 20, 771–779.38. Andrade, C.G.; Figueiredo, R.C.B.Q.; Ribeiro, K.R.C.; Souza, L.I.O.; Sarmento-Neto, J.F.; Rebouças, J.S.; Santos, B.S.; Ribeiro, M.S.; Carvalho, L.B.; Fontes, A. Photodynamic e ect of zinc porphyrin on the promastigote and amastigote forms of Leishmania braziliensis. Photochem. Photobiol. Sci. 2018, 17, 482–490.39. Espitia-Almeida, F.; Díaz-Uribe, C.; Vallejo,W.; Gómez-Camargo, D.; Romero-Bohorquez, A.R.; Schott, E.; Zarate, X. Synthesis and Characterization of 5,10,15,20-Tetrakis(4-ethylphenyl)porphyrin and (Zn2+, Mn2+, Sn2+, Ni2+, Al3+, V3+)-Derivatives: Photophysical and DFT study. ChemistrySelect 2019, 4, 6290–6294.40. Dube, E.; Nwaji, N.; Oluwole, D.O.; Mack, J.; Nyokong, T. Investigation of photophysicochemical properties of zinc phthalocyanines conjugated to metallic nanoparticles. J. Photochem. Photobiol. A Chem. 2017, 349, 148–161.41. Zoltan, T.; Vargas, F.; López, V.; Chávez, V.; Rivas, C.; Ramírez, Á.H. Influence of charge and metal coordination of meso-substituted porphyrins on bacterial photoinactivation. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2015, 135, 747–756.42. Guillaumot, D.; Issawi, M.; Da Silva, A.; Leroy-Lhez, S.; Sol, V.; Riou, C. Synergistic enhancement of tolerance mechanisms in response to photoactivation of cationic tetra (N-methylpyridyl) porphyrins in tomato plantlets. JPB 2016, 156, 69–78.43. Bonnett, R. Chemical Aspects of Photodynamic Therapy; CRC Press: Boca Raton, FL, USA, 2014; ISBN 9781482296952.44. Pummer, A.; Knüttel, H.; Hiller, K.-A.; Buchalla,W.; Cieplik, F.; Maisch, T. Antimicrobial e cacy of irradiation with visible light on oral bacteria in vitro: A systematic review. Future Med. Chem. 2017, 9, 1557–1574.45. Ribeiro, A.P.D.; Andrade, M.C.; Bagnato, V.S.; Vergani, C.E.; Primo, F.L.; Tedesco, A.C.; Pavarina, A.C. Antimicrobial photodynamic therapy against pathogenic bacterial suspensions and biofilms using chloro-aluminum phthalocyanine encapsulated in nanoemulsions. Lasers Med. Sci. 2015, 30, 549–559.46. Song, D.; Lindoso, J.A.L.; Oyafuso, L.K.; Kanashiro, E.H.Y.; Cardoso, J.L.; Uchoa, A.F.; Tardivo, J.P.; Baptista, M.S. Photodynamic Therapy Using Methylene Blue to Treat Cutaneous Leishmaniasis. Photomed. Laser Surg. 2011, 29, 711–715.47. Hernández, I.P.; Montanari, J.; Valdivieso, W.; Morilla, M.J.; Romero, E.L.; Escobar, P. In vitro phototoxicity of ultradeformable liposomes containing chloroaluminum phthalocyanine against New World Leishmania species. J. Photochem. Photobiol. B Biol. 2012, 117, 157–163.48. Piñero, J.E.; Jiménez, I.A.; Valladares, B.; Ravelo, Á.G. Advances in leishmaniasis chemotherapy and new relevant patents. Expert Opin. Ther. Pat. 2004, 14, 1113–1123.49. Chakravarty, J.; Sundar, S. Drug Resistance in Leishmaniasis. J. Glob. Infect. Dis. 2010, 2, 167.50. Croft, S.L.; Sundar, S.; Fairlamb, A.H. Drug Resistance in Leishmaniasis. Clin. Microbiol. Rev. 2006, 19, 111–126.51. Ouellette, M.; Papadopoulou, B. Mechanisms of drug resistance in Leishmania. Parasitol. Today 1993, 9, 150–153.52. Skovsen, E.; Snyder, J.W.; Lambert, J.D.; Ogilby, P.R. Lifetime and Di usion of Singlet Oxygen in a Cell. J. Phys. Chesm. B 2005, 109, 8570–8573.53. Halliwell, B.; Gutteridge, J.M.C. Free Radicals in Biology and Medicine, 2nd ed.; Oxford University Press: New York, NY, USA, 2015; ISBN 9780198717485.54. Breitenbach, T.; Kuimova, M.K.; Gbur, P.; Hatz, S.; Schack, N.B.; Pedersen, B.W.; Lambert, J.D.C.; Poulsen, L.; Ogilby, P.R. Photosensitized production of singlet oxygen: Spatially-resolved optical studies in single cells. Photochem. Photobiol. Sci. 2009, 8, 442–452.55. Adler, A.D.; Longo, F.R.; Shergalis, W. Mechanistic Investigations of Porphyrin Syntheses. I. Preliminary Studies on ms-Tetraphenylporphin. J. Am. Chem. Soc. 1964, 86, 3145–3149.56. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55–63.57. Taylor, V.M.; Muñoz, D.L.; Cedeño, D.L.; Vélez, I.D.; Jones, M.A.; Robledo, S.M. Leishmania tarentolae: Utility as an in vitro model for screening of antileishmanial agents. Exp. Parasitol. 2010, 126, 471–475.58. Akilov, O.E.; Kosaka, S.; O’Riordan, K.; Hasan, T. Parasiticidal e ect of -aminolevulinic acid-based photodynamic therapy for cutaneous leishmaniasis is indirect and mediated through the killing of the host cells. Exp. Dermatol. 2007, 16, 651–660.59. Kiderlen, A.F.; Kaye, P.M.Amodified colorimetric assay of macrophage activation for intracellular cytotoxicity against Leishmania parasites. J. Immunol. Methods 1990, 127, 11–18.60. Durmu¸s, M.; Nyokong, T. Photophysicochemical and fluorescence quenching studies of benzyloxyphenoxy-substituted zinc phthalocyanines. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2008, 69, 1170–1177.61. Khodov, I.A.; Nikiforov, M.Y.; Alper, G.A.; Mamardashvili, G.M.; Mamardashvili, N.Z.; Koifman, O.I. Synthesis and spectroscopic characterization of Ru(II) and Sn(IV)-porphyrins supramolecular complexes. J. Mol. Struct. 2015, 1081, 426–430.62. Moreira, M.E.C.; Del Portillo, H.A.; Milder, R.V.; Balanco, J.M.F.; Barcinski, M.A. Heat shock induction of apoptosis in promastigotes of the unicellular organism Leishmania (Leishmania) amazonensis. J. Cell. Physiol. 1996, 167, 305–313.http://purl.org/coar/resource_type/c_6501ORIGINALmolecules-25-01887.pdfmolecules-25-01887.pdfapplication/pdf2072036https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/965/1/molecules-25-01887.pdfc1e9fe83f3557aee7a9397f11df71868MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/965/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/965/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/965oai:repositorio.uniatlantico.edu.co:20.500.12834/9652022-11-15 16:16:15.518DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.coVMOpcm1pbm9zIGdlbmVyYWxlcyBkZWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbCBkZSBsYSBVbml2ZXJzaWRhZCBkZWwgQXRsw6FudGljbwoKRWwgKGxvcykgYXV0b3IgKGVzKSBoYW4gYXNlZ3VyYWRvIChuKSBsbyBzaWd1aWVudGUgc29icmUgbGEgb2JyYSBhIGludGVncmFyIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwsIHF1ZToKCuKXjwlFcyBvcmlnaW5hbCwgZGUgc3UgZXhjbHVzaXZhIGF1dG9yw61hLCBzZSByZWFsaXrDsyBzaW4gdmlvbGFyIG8gdXN1cnBhciBkZXJlY2hvcyBkZSBhdXRvciBkZSB0ZXJjZXJvcyB5IHBvc2VlIGxhIHRpdHVsYXJpZGFkLgril48JQXN1bWlyw6FuIGxhIHJlc3BvbnNhYmlsaWRhZCB0b3RhbCBwb3IgZWwgY29udGVuaWRvIGEgbGEgb2JyYSBhbnRlIGxhIEluc3RpdHVjacOzbiB5IHRlcmNlcm9zLgril48JQXV0b3JpemFuIGEgdMOtdHVsbyBncmF0dWl0byB5IHJlbnVuY2lhcyBhIHJlY2liaXIgZW1vbHVtZW50b3MgcG9yIGxhcyBhY3RpdmlkYWRlcyBxdWUgc2UgcmVhbGljZW4gY29uIGVsbGEsIHNlZ8O6biBzdSBsaWNlbmNpYS4KCgpMYSBVbml2ZXJzaWRhZCBkZWwgQXRsw6FudGljbywgcG9yIHN1IHBhcnRlLCBzZSBjb21wcm9tZXRlIGEgYWN0dWFyIGVuIGxvcyB0w6lybWlub3MgZXN0YWJsZWNpZG9zIGVuIGxhIExleSAyMyBkZSAxOTgyIHkgbGEgRGVjaXNpw7NuIEFuZGluYSAzNTEgZGUgMTk5MywgZGVtw6FzIG5vcm1hcyBnZW5lcmFsZXMgc29icmUgbGEgbWF0ZXJpYSB5IGVsIEFjdWVyZG8gU3VwZXJpb3IgMDAxIGRlIDE3IGRlIG1hcnpvIGRlIDIwMTEsIHBvciBtZWRpbyBkZWwgY3VhbCBzZSBleHBpZGUgZWwgRXN0YXR1dG8gZGUgUHJvcGllZGFkIEludGVsZWN0dWFsIGRlIGxhIFVuaXZlcnNpZGFkIGRlbCBBdGzDoW50aWNvLgoKUG9yIMO6bHRpbW8sIGhhbiBzaWRvIGluZm9ybWFkb3Mgc29icmUgZWwgdHJhdGFtaWVudG8gZGUgZGF0b3MgcGVyc29uYWxlcyBwYXJhIGZpbmVzIGFjYWTDqW1pY29zIHkgZW4gYXBsaWNhY2nDs24gZGUgY29udmVuaW9zIGNvbiB0ZXJjZXJvcyBvIHNlcnZpY2lvcyBjb25leG9zIGNvbiBhY3RpdmlkYWRlcyBwcm9waWFzIGRlIGxhIGFjYWRlbWlhLCBiYWpvIGVsIGVzdHJpY3RvIGN1bXBsaW1pZW50byBkZSBsb3MgcHJpbmNpcGlvcyBkZSBsZXkuCgpMYXMgY29uc3VsdGFzLCBjb3JyZWNjaW9uZXMgeSBzdXByZXNpb25lcyBkZSBkYXRvcyBwZXJzb25hbGVzIHB1ZWRlbiBwcmVzZW50YXJzZSBhbCBjb3JyZW8gZWxlY3Ryw7NuaWNvIGhhYmVhc2RhdGFAbWFpbC51bmlhdGxhbnRpY28uZWR1LmNvCg==

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