Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications

The design of sca olding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients. Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properti...

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Autores:
Grande Tovar, Carlos David
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Atlántico
Repositorio:
Repositorio Uniatlantico
Idioma:
eng
OAI Identifier:
oai:repositorio.uniatlantico.edu.co:20.500.12834/961
Acceso en línea:
https://hdl.handle.net/20.500.12834/961
Palabra clave:
amide; biodegradable films; chitosan-grafted carbon nano-onions; poly (vinyl alcohol); tissue engineering
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openAccess
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http://creativecommons.org/licenses/by-nc/4.0/
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dc.title.spa.fl_str_mv Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
title Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
spellingShingle Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
amide; biodegradable films; chitosan-grafted carbon nano-onions; poly (vinyl alcohol); tissue engineering
title_short Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
title_full Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
title_fullStr Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
title_full_unstemmed Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
title_sort Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
dc.creator.fl_str_mv Grande Tovar, Carlos David
dc.contributor.author.none.fl_str_mv Grande Tovar, Carlos David
dc.contributor.other.none.fl_str_mv Castro, Jorge Iván
Valencia, Carlos Humberto
Navia Porras, Diana Paola
Mina Hernandez, José Herminsul
Valencia, Mayra Eliana
Chaur, Manuel N.
dc.subject.keywords.spa.fl_str_mv amide; biodegradable films; chitosan-grafted carbon nano-onions; poly (vinyl alcohol); tissue engineering
topic amide; biodegradable films; chitosan-grafted carbon nano-onions; poly (vinyl alcohol); tissue engineering
description The design of sca olding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients. Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of di erent materials and devices. In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond. These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats. Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes. Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Di erential Scanning Calorimetry (DSC), Tensile strength, X-ray Di raction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM). The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment. Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding. On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations. SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO. With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-03-07
dc.date.submitted.none.fl_str_mv 2020-02-03
dc.date.accessioned.none.fl_str_mv 2022-11-15T21:15:19Z
dc.date.available.none.fl_str_mv 2022-11-15T21:15:19Z
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.hasVersion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.spa.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12834/961
dc.identifier.doi.none.fl_str_mv 10.3390/molecules25051203
dc.identifier.instname.spa.fl_str_mv Universidad del Atlántico
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad del Atlántico
url https://hdl.handle.net/20.500.12834/961
identifier_str_mv 10.3390/molecules25051203
Universidad del Atlántico
Repositorio Universidad del Atlántico
dc.language.iso.spa.fl_str_mv eng
language eng
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dc.publisher.place.spa.fl_str_mv Barranquilla
dc.publisher.sede.spa.fl_str_mv Sede Norte
dc.source.spa.fl_str_mv MDPI AG
institution Universidad del Atlántico
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spelling Grande Tovar, Carlos David9e800b3a-a886-44a8-9872-71464aa6e429Castro, Jorge IvánValencia, Carlos HumbertoNavia Porras, Diana PaolaMina Hernandez, José HerminsulValencia, Mayra ElianaChaur, Manuel N.2022-11-15T21:15:19Z2022-11-15T21:15:19Z2020-03-072020-02-03https://hdl.handle.net/20.500.12834/96110.3390/molecules25051203Universidad del AtlánticoRepositorio Universidad del AtlánticoThe design of sca olding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients. Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of di erent materials and devices. In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond. These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats. Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes. Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Di erential Scanning Calorimetry (DSC), Tensile strength, X-ray Di raction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM). The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment. Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding. On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations. SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO. With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2MDPI AGNanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical ApplicationsPúblico generalamide; biodegradable films; chitosan-grafted carbon nano-onions; poly (vinyl alcohol); tissue engineeringinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede Norte1. Hollister, S.J. Porous sca old design for tissue engineering. Nat. Mater. 2005, 4, 518–524.2. Chen, G.; Ushida, T.; Tateishi, T. Sca old design for tissue engineering. Macromol. Biosci. 2002, 2, 67–77.3. Eivazzadeh-Keihan, R.; Maleki, A.; de la Guardia, M.; Bani, M.S.; Chenab, K.K.; Pashazadeh-Panahi, P.; Baradaran, B.; Mokhtarzadeh, A.; Hamblin, M.R. Carbon based nanomaterials for tissue engineering of bone: Building new bone on small black sca olds: A review. J. Adv. Res. 2019, 18, 185–201.4. Harrison, B.S.; Atala, A. Carbon nanotube applications for tissue engineering. Biomaterials 2007, 28, 344–353.5. Gerasimenko, A.Y.; Ichkitidze, L.P.; Podgaetsky, V.M.; Selishchev, S.V. Biomedical applications of promising nanomaterials with carbon nanotubes. Biomed. Eng. 2015, 48, 310–314.6. Shin, S.R.; Li, Y.-C.; Jang, H.L.; Khoshakhlagh, P.; Akbari, M.; Nasajpour, A.; Zhang, Y.S.; Tamayol, A.; Khademhosseini, A. Graphene-based materials for tissue engineering. Adv. Drug Deliv. Rev. 2016, 105, 255–274.7. Yu, X.; Tang, X.; Gohil, S.V.; Laurencin, C.T. Biomaterials for bone regenerative engineering. Adv. Healthc. Mater. 2015, 4, 1268–1285.8. Stratton, S.; Shelke, N.B.; Hoshino, K.; Rudraiah, S.; Kumbar, S.G. Bioactive polymeric sca olds for tissue engineering. Bioact. Mater. 2016, 1, 93–108.9. Dhandayuthapani, B.; Yoshida, Y.; Maekawa, T.; Kumar, D.S. Polymeric sca olds in tissue engineering application: A review. Int. J. Polym. Sci. 2011, 2011, 290602.10. Venkatesan, J.; Bhatnagar, I.; Manivasagan, P.; Kang, K.-H.; Kim, S.-K. Alginate composites for bone tissue engineering: A review. Int. J. Biol. Macromol. 2015, 72, 269–281.11. Saravanan, S.; Leena, R.S.; Selvamurugan, N. Chitosan based biocomposite sca olds for bone tissue engineering. Int. J. Biol. Macromol. 2016, 93, 1354–1365.12. Niranjan, R.; Koushik, C.; Saravanan, S.; Moorthi, A.; Vairamani, M.; Selvamurugan, N. A novel injectable temperature-sensitive zinc doped chitosan/ -glycerophosphate hydrogel for bone tissue engineering. Int. J. Biol. Macromol. 2013, 54, 24–29.13. Shui,W.; Zhang,W.; Yin, L.; Nan, G.; Liao, Z.; Zhang, H.;Wang, N.;Wu, N.; Chen, X.;Wen, S. Characterization of sca old carriers for BMP9-transduced osteoblastic progenitor cells in bone regeneration. J. Biomed. Mater. Res. Part. A 2014, 102, 3429–3438.14. McFadden, T.M.; Du y, G.P.; Allen, A.B.; Stevens, H.Y.; Schwarzmaier, S.M.; Plesnila, N.; Murphy, J.M.; Barry, F.P.; Guldberg, R.E.; O’brien, F.J. The delayed addition of human mesenchymal stem cells to pre-formed endothelial cell networks results in functional vascularization of a collagen–glycosaminoglycan sca old in vivo. Acta Biomater. 2013, 9, 9303–9316.15. Lin, C.-Y.; Chang, Y.-H.; Li, K.-C.; Lu, C.-H.; Sung, L.-Y.; Yeh, C.-L.; Lin, K.-J.; Huang, S.-F.; Yen, T.-C.; Hu, Y.-C. The use of ASCs engineered to express BMP2 or TGF- 3 within sca old constructs to promote calvarial bone repair. Biomaterials 2013, 34, 9401–9412.16. Sun, Y.; Jiang, Y.; Liu, Q.; Gao, T.; Feng, J.Q.; Dechow, P.; D’Souza, R.N.; Qin, C.; Liu, X. Biomimetic engineering of nanofibrous gelatin sca olds with noncollagenous proteins for enhanced bone regeneration. Tissue Eng. Part. A. 2013, 19, 1754–1763.17. Saravanan, S.; Nethala, S.; Pattnaik, S.; Tripathi, A.; Moorthi, A.; Selvamurugan, N. Preparation, characterization and antimicrobial activity of a bio-composite sca old containing chitosan/nano-hydroxyapatite/nano-silver for bone tissue engineering. Int. J. Biol. Macromol. 2011, 49, 188–193.18. Khor, E.; Lim, L.Y. Implantable applications of chitin and chitosan. Biomaterials 2003, 24, 2339–2349.19. Soundarya, S.P.; Menon, A.H.; Chandran, S.V.; Selvamurugan, N. Bone tissue engineering: Sca old preparation using chitosan and other biomaterials with di erent design and fabrication techniques. Int. J. Biol. Macromol. 2018, 119, 1228–1239.20. Dhivya, S.; Keshav Narayan, A.; Logith Kumar, R.; Viji Chandran, S.; Vairamani, M.; Selvamurugan, N. Proliferation and di erentiation of mesenchymal stem cells on sca olds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering. Cell Prolif. 2018, 51, e12408.21. Shamekhi, M.A.; Mirzadeh, H.; Mahdavi, H.; Rabiee, A.; Mohebbi-Kalhori, D.; Eslaminejad, M.B. Graphene oxide containing chitosan sca olds for cartilage tissue engineering. Int. J. Biol. Macromol. 2019, 127, 396–405.22. Kashi, M.; Baghbani, F.; Moztarzadeh, F.; Mobasheri, H.; Kowsari, E. Green synthesis of degradable conductive thermosensitive oligopyrrole/chitosan hydrogel intended for cartilage tissue engineering. Int. J. Biol. Macromol. 2018, 107, 1567–1575.23. Ahmad, M.; Manzoor, K.; Ahmad, S.; Akram, N.; Ikram, S. Chitosan-based nanocomposites for cardiac, liver, and wound healing applications. In Applications of Nanocomposite Materials in Orthopedics; Elsevier: Amsterdam, The Netherlands, 2019; pp. 253–262.24. Wu, G.; Deng, X.; Song, J.; Chen, F. Enhanced biological properties of biomimetic apatite fabricated polycaprolactone/chitosan nanofibrous bio-composite for tendon and ligament regeneration. J. Photochem. Photobiol. B Biol. 2018, 178, 27–32.25. Chen, E.; Yang, L.; Ye, C.; Zhang, W.; Ran, J.; Xue, D.; Wang, Z.; Pan, Z.; Hu, Q. An asymmetric chitosan sca old for tendon tissue engineering: In vitro and in vivo evaluation with rat tendon stem/progenitor cells. Acta Biomater. 2018, 73, 377–387.26. Qasim, S.; Zafar, M.; Najeeb, S.; Khurshid, Z.; Shah, A.; Husain, S.; Rehman, I. Electrospinning of chitosan-based solutions for tissue engineering and regenerative medicine. Int. J. Mol. Sci. 2018, 19, 407.27. González-Quevedo, D.; Martínez-Medina, I.; Campos, A.; Campos, F.; Carriel, V. Tissue engineering strategies for the treatment of tendon injuries: A systematic review and meta-analysis of animal models. Bone Jt. Res. 2018, 7, 318–324.28. Ueno, H.; Mori, T.; Fujinaga, T. Topical formulations and wound healing applications of chitosan. Adv. Drug Deliv. Rev. 2001, 52, 105–115.29. Ratner, B.D.; Ho man, A.S.; Schoen, F.J.; Lemons, J.E. Biomaterials Science: An Introduction to Materials in Medicine; Elsevier: Amsterdam, The Netherlands, 2004; ISBN 008047036X.30. Thakur, V.K.; Voicu, S.I. Recent advances in cellulose and chitosan based membranes for water purification: A concise review. Carbohydr. Polym. 2016, 146, 148–165.31. He, Y.; Miao, J.; Chen, S.; Zhang, R.; Zhang, L.; Tang, H.; Yang, H. Preparation and characterization of a novel positively charged composite hollow fiber nanofiltration membrane based on chitosan lactate. Rsc Adv. 2019, 9, 4361–4369.32. Medina, V.F.; Griggs, C.S.; Mattei-Sosa, J.; Petery, B.; Gurtowski, L. Advanced Filtration Membranes Using Chitosan and Graphene Oxide. U.S. Patent Application 20190039026, 7 February 2019.33. Sun, T.; Guo, X.; Zhong, R.; Ma, L.; Li, H.; Gu, Z.; Guan, J.; Tan, H.; You, C.; Tian, M. Interactions of oligochitosan with blood components. Int. J. Biol. Macromol. 2019, 124, 304–313.34. Heise, K.; Hobisch, M.; Sacarescu, L.; Maver, U.; Hobisch, J.; Reichelt, T.; Sega, M.; Fischer, S.; Spirk, S. Low-molecular-weight sulfonated chitosan as template for anticoagulant nanoparticles. Int. J. Nanomed. 2018, 13, 4881–4894.35. Guo, X.; Sun, T.; Zhong, R.; Ma, L.; You, C.; Tian, M.; Li, H.; Wang, C. E ects of chitosan oligosaccharides on human blood components. Front. Pharmacol. 2018, 9, 1412.36. Dimassi, S.; Tabary, N.; Chai, F.; Blanchemain, N.; Martel, B. Sulfonated and sulfated chitosan derivatives for biomedical applications: A review. Carbohydr. Polym. 2018, 202, 382–396.37. Mehta, P.; Al-Kinani, A.A.; Arshad, M.S.; Singh, N.; van der Merwe, S.M.; Chang, M.-W.; Alany, R.G.; Ahmad, Z. Engineering and development of chitosan-based Nanocoatings for Ocular Contact Lenses. J. Pharm. Sci. 2019, 108, 1540–1551.38. Ali, A.; Ahmed, S. A review on chitosan and its nanocomposites in drug delivery. Int. J. Biol. Macromol. 2018, 109, 273–286.39. Ahsan, S.M.; Thomas, M.; Reddy, K.K.; Sooraparaju, S.G.; Asthana, A.; Bhatnagar, I. Chitosan as biomaterial in drug delivery and tissue engineering. Int. J. Biol. Macromol. 2018, 110, 97–109.40. Gomillion, C.T. Assessing the potential of chitosan/polylactide nanoparticles for delivery of therapeutics for triple-negative breast cancer treatment. Regen. Eng. Transl. Med. 2019, 5, 61–73.41. Raval, R.; Rangnekar, R.H.; Raval, K. Optimization of chitosan nanoparticles synthesis and its applications in fatty acid absorption. In Materials, Energy and Environment Engineering; Springer: Berlin/Heidelberg, Germany; pp. 253–256.42. Berkland, C.; Qian, J.; Sullivan, B.P. Micelle Sequestering Polymers. U.S. Patent Application 20150216896, 6 August 2015.43. Hamedi, H.; Moradi, S.; Hudson, S.M.; Tonelli, A.E. Chitosan based hydrogels and their applications for drug delivery in wound dressings: A review. Carbohydr. Polym. 2018, 199, 445–460.44. Mohebbi, S.; Nezhad, M.N.; Zarrintaj, P.; Jafari, S.H.; Gholizadeh, S.S.; Saeb, M.R.; Mozafari, M. Chitosan in biomedical engineering: A critical review. Curr. Stem Cell Res. Ther. 2019, 14, 93–116.45. Cazón, P.; Vázquez, M. Applications of Chitosan as Food Packaging Materials. In Sustainable Agriculture Reviews 36; Springer: Berlin/Heidelberg, Germany, 2019; pp. 81–123.46. Wang, H.; Qian, J.; Ding, F. Emerging chitosan-based films for food packaging applications. J. Agric. Food Chem. 2018, 66, 395–413.47. HPS, A.K.; Saurabh, C.K.; Adnan, A.S.; Fazita, M.R.N.; Syakir, M.I.; Davoudpour, Y.; Rafatullah, M.; Abdullah, C.K.; Haafiz, M.K.M.; Dungani, R. A review on chitosan-cellulose blends and nanocellulose reinforced chitosan biocomposites: Properties and their applications. Carbohydr. Polym. 2016, 150, 216–226.48. Koosha, M.; Mirzadeh, H.; Shokrgozar, M.A.; Farokhi, M. Nanoclay-reinforced electrospun chitosan/PVA nanocomposite nanofibers for biomedical applications. Rsc Adv. 2015, 5, 10479–10487.49. Umeyama, T.; Imahori, H. Photofunctional hybrid nanocarbon materials. J. Phys. Chem. C 2012, 117, 3195–3209.50. Rettenbacher, A.S.; Elliott, B.; Hudson, J.S.; Amirkhanian, A.; Echegoyen, L. Preparation and functionalization of multilayer fullerenes (carbon nano-onions). Chem. Eur. J. 2006, 12, 376–387.51. Hirata, A.; Igarashi, M.; Kaito, T. Study on solid lubricant properties of carbon onions produced by heat treatment of diamond clusters or particles. Tribol. Int. 2004, 37, 899–905.52. Ibáñez-Redín, G.; Furuta, R.H.M.; Wilson, D.; Shimizu, F.M.; Materon, E.M.; Arantes, L.M.R.B.; Melendez, M.E.; Carvalho, A.L.; Reis, R.M.; Chaur, M.N. Screen-printed interdigitated electrodes modified with nanostructured carbon nano-onion films for detecting the cancer biomarker CA19-9. Mater. Sci. Eng. C 2019, 99, 1502–1508.53. Ding, L.; Stilwell, J.; Zhang, T.; Elboudwarej, O.; Jiang, H.; Selegue, J.P.; Cooke, P.A.; Gray, J.W.; Chen, F.F. Molecular characterization of the cytotoxic mechanism of multiwall carbon nanotubes and nano-onions on human skin fibroblast. Nano Lett. 2005, 5, 2448–2464.54. Fan, J.; Grande, C.D.; Rodrigues, D.F. Biodegradation of graphene oxide-polymer nanocomposite films in wastewater. Environ. Sci. Nano 2017, 4, 1808–1816.55. Grande, C.D.; Mangadlao, J.; Fan, J.; De Leon, A.; Delgado-Ospina, J.; Rojas, J.G.; Rodrigues, D.F.; Advincula, R. Chitosan cross-linked graphene oxide nanocomposite films with antimicrobial activity for application in food industry. Macromol. Symp. 2017, 374, 1600114.56. Ruiz, S.; Tamayo, A.J.; Delgado Ospina, J.; Navia Porras, P.D.; Valencia Zapata, E.M.; Mina Hernandez, H.J.; Valencia, H.C.; Zuluaga, F.; Grande Tovar, D.C. Antimicrobial films based on nanocomposites of chitosan/poly(vinyl alcohol)/graphene oxide for biomedical applications. Biomolecules 2019, 9, 109.57. López Tenorio, D.; Valencia, H.C.; Valencia, C.; Zuluaga, F.; Valencia, E.M.; Mina, H.J.; Grande Tovar, D.C. Evaluation of the biocompatibility of CS-Graphene oxide compounds in vivo. Int. J. Mol. Sci. 2019, 20, 1572.58. Valencia, C.; Valencia, C.; Zuluaga, F.; Valencia, M.; Mina, J.; Grande-Tovar, C. Synthesis and application of sca olds of chitosan-graphene oxide by the freeze-drying method for tissue regeneration. Molecules 2018, 23, 2651.59. Tamayo Marín, A.J.; Londoño, R.S.; Delgado, J.; Navia Porras, P.D.; Valencia Zapata, E.M.; Mina Hernandez, H.J.; Valencia, H.C.; Grande Tovar, D.C. Biocompatible and antimicrobial electrospun membranes based on nanocomposites of chitosan/poly (vinyl alcohol)/graphene oxide. Int. J. Mol. Sci. 2019, 20, 2987.60. Valencia Zapata, E.M.; Mina Hernandez, H.J.; Grande Tovar, D.C.; Valencia Llano, H.C.; Diaz Escobar, A.J.; Vázquez-Lasa, B.; San Román, J.; Rojo, L. Novel bioactive and antibacterial acrylic bone cement nanocomposites modified with graphene oxide and chitosan. Int. J. Mol. Sci. 2019, 20, 2938.61. Grande Tovar, C.D.; Castro, J.I.; Valencia, C.H.; Navia Porras, D.P.; Hernandez, M.; Herminsul, J.; Valencia, M.E.; Velásquez, J.D.; Chaur, M.N. Preparation of chitosan/poly (vinyl alcohol) nanocomposite films incorporated with oxidized carbon nano-onions (multi-layer fullerenes) for tissue-engineering applications. Biomolecules 2019, 9, 684.62. Wu, Z.; Feng,W.; Feng, Y.; Liu, Q.; Xu, X.; Sekino, T.; Fujii, A.; Ozaki, M. Preparation and characterization of chitosan-grafted multiwalled carbon nanotubes and their electrochemical properties. Carbon 2007, 45, 1212–1218.63. Osswald, S.; Havel, M.; Gogotsi, Y. Monitoring oxidation of multiwalled carbon nanotubes by Raman spectroscopy. J. Raman Spectrosc. 2007, 38, 728–736.64. Shriner, R.L.; Fuson, R.C.; Curtin, D.Y. The systematic identification of organic compounds; John Wiley Sons: New York, NY, USA, 1948; pp. 202–207.65. Ke, G.; Guan,W.; Tang, C.; Guan,W.; Zeng, D.; Deng, F. Covalent functionalization of multiwalled carbon nanotubes with a low molecular weight chitosan. Biomacromolecules 2007, 8, 322–326.66. Mallakpour, S.; Zadehnazari, A. A facile, e cient, and rapid covalent functionalization of multi-walled carbon nanotubes with natural amino acids under microwave irradiation. Prog. Org. Coat. 2014, 77, 679–684.67. Chattopadhyay, J.; Mukherjee, A.; Chakraborty, S.; Kang, J.; Loos, P.J.; Kelly, K.F.; Schmidt, H.K.; Billups,W.E. Exfoliated soluble graphite. Carbon 2009, 47, 2945–2949.68. Bustos-Ramírez, K.; Martínez-Hernández, A.L.; Martínez-Barrera, G.; Icaza, M.D.; Castaño, V.M.; Velasco-Santos, C. Covalently bonded chitosan on graphene oxide via redox reaction. Materials 2013, 6, 911–926.69. Cio , C.T.; Palkar, A.; Melin, F.; Kumbhar, A.; Echegoyen, L.; Melle-Franco, M.; Zerbetto, F.; Rahman, G.M.A.; Ehli, C.; Sgobba, V. A carbon nano-onion–ferrocene donor–acceptor system: Synthesis, characterization and properties. Chem. Eur. J. 2009, 15, 4419–4427.70. Carson, L.; Kelly-Brown, C.; Stewart, M.; Oki, A.; Regisford, G.; Luo, Z.; Bakhmutov, V.I. Synthesis and characterization of chitosan–carbon nanotube composites. Mater. Lett. 2009, 63, 617–620.71. Srinivasa, P.C.; Ramesh, M.N.; Kumar, K.R.; Tharanathan, R.N. Properties and sorption studies of chitosan–polyvinyl alcohol blend films. Carbohydr. Polym. 2003, 53, 431–438.72. Pandele, A.M.; Ionita, M.; Crica, L.; Dinescu, S.; Costache, M.; Iovu, H. Synthesis, characterization, and in vitro studies of graphene oxide/chitosan-polyvinyl alcohol films. Carbohydr. Polym. 2014, 102, 813–820.73. Jia, Y.-T.; Gong, J.; Gu, X.-H.; Kim, H.-Y.; Dong, J.; Shen, X.-Y. Fabrication and characterization of poly (vinyl alcohol)/chitosan blend nanofibers produced by electrospinning method. Carbohydr. Polym. 2007, 67, 403–409.74. Lu, L.; Peng, F.; Jiang, Z.; Wang, J. Poly(vinyl alcohol)/chitosan blend membranes for pervaporation of benzene/cyclohexane mixtures. J. Appl. Polym. Sci. 2006, 101, 167–173.75. Yadav, I.; Nayak, S.K.; Rathnam, V.S.S.; Banerjee, I.; Ray, S.S.; Anis, A.; Pal, K. Reinforcing e ect of graphene oxide reinforcement on the properties of poly (vinyl alcohol) and carboxymethyl tamarind gum based phase-separated film. J. Mech. Behav. Biomed. Mater. 2018, 81, 61–71.76. Yang, X.; Tu, Y.; Li, L.; Shang, S.; Tao, X. Well-dispersed chitosan/graphene oxide nanocomposites. Acs Appl. Mater. Interfaces 2010, 2, 1707–1713.77. Espigares, I.; Elvira, C.; Mano, J.F.; Vázquez, B.; San Román, J.; Reis, R.L. New partially degradable and bioactive acrylic bone cements based on starch blends and ceramic fillers. Biomaterials 2002, 23, 1883–1895.78. Herath, H.M.T.U.; Di Silvio, L.; Evans, J.R.G. Biological evaluation of solid freeformed, hard tissue sca olds for orthopedic applications. J. Appl. Biomater. Biomech. 2010, 8, 89–96.79. Figueira Maldonado, E. Degradación hidrolítica a diferentespHde un material compuesto Poli(ácido láctico)/Quitosano, Proyecto de grado; Universidad Simón Bolívar, Sartenejas: Caracas, Venezuela, 2008.80. Depan, D.; Shah, J.S.; Misra, R.D.K. Degradation mechanism and increased stability of chitosan-based hybrid sca olds cross-linked with nanostructured carbon: Process-structure-functional property relationship. Polym. Degrad. Stab. 2013, 98, 2331–2339.81. Maruyama, M.; Ito, M. In vitro properties of a chitosan-bonded self-hardening paste with hydroxyapatite granules. J. Biomed. Mater. Res. 1996, 32, 527–532.82. Tomihata, K.; Ikada, Y. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials 1997, 18, 567–575.83. Pella, M.C.G.; Lima-Tenório, M.K.; Tenorio-Neto, E.T.; Guilherme, M.R.; Muniz, E.C.; Rubira, A.F. Chitosan-based hydrogels: From preparation to biomedical applications. Carbohydr. Polym. 2018, 196, 233–245.84. Fujita, M.; Ishihara, M.; Simizu, M.; Obara, K.; Ishizuka, T.; Saito, Y.; Yura, H.; Morimoto, Y.; Takase, B.; Matsui, T. Vascularization in vivo caused by the controlled release of fibroblast growth factor-2 from an injectable chitosan/non-anticoagulant heparin hydrogel. Biomaterials 2004, 25, 699–706.85. Sok, V.; Fragoso, A. Preparation and characterization of alkaline phosphatase, horseradish peroxidase, and glucose oxidase conjugates with carboxylated carbon nano-onions. Prep. Biochem. Biotechnol. 2018, 48, 136–143.86. Vatanpour, V.; Safarpour, M.; Khataee, A.; Zarrabi, H.; Yekavalangi, M.E.; Kavian, M. A thin film nanocomposite reverse osmosis membrane containing amine-functionalized carbon nanotubes. Sep. Purif. Technol. 2017, 184, 135–143.http://purl.org/coar/resource_type/c_6501ORIGINALmolecules-25-01203.pdfmolecules-25-01203.pdfapplication/pdf8896628https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/961/1/molecules-25-01203.pdf5d839532611298005cc6a91ff999f032MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/961/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/961/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/961oai:repositorio.uniatlantico.edu.co:20.500.12834/9612022-11-15 16:15:20.256DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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