Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications
Graphene oxide (GO) has recently gained attention as a scaffold reinforcing agent for tissue engineering. Biomechanical and biological properties through a synergistic effect can be strengthened when combined with other materials such as chitosan (CS). For that reason, chitosan was used for Graphene...
- Autores:
-
Valencia, Ana María
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/1145
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/1145
- Palabra clave:
- Chitosan Graphene oxide Regenerative medicine Tissue engineering
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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dc.title.spa.fl_str_mv |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
dc.title.alternative.spa.fl_str_mv |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
title |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
spellingShingle |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications Chitosan Graphene oxide Regenerative medicine Tissue engineering |
title_short |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
title_full |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
title_fullStr |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
title_full_unstemmed |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
title_sort |
Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications |
dc.creator.fl_str_mv |
Valencia, Ana María |
dc.contributor.author.none.fl_str_mv |
Valencia, Ana María |
dc.contributor.other.none.fl_str_mv |
Valencia, Carlos Humberto Zuluaga, Fabio Grande-Tovar, Carlos David |
dc.subject.keywords.spa.fl_str_mv |
Chitosan Graphene oxide Regenerative medicine Tissue engineering |
topic |
Chitosan Graphene oxide Regenerative medicine Tissue engineering |
description |
Graphene oxide (GO) has recently gained attention as a scaffold reinforcing agent for tissue engineering. Biomechanical and biological properties through a synergistic effect can be strengthened when combined with other materials such as chitosan (CS). For that reason, chitosan was used for Graphene Oxide (GO) functionalization through an amide group whose formation was evident by bands around 1600 cm 1 in the FTIR analysis. Furthermore, bands located at 1348 cm 1 (D band), 1593 cm 1 (G band), and 2416 cm 1 (2D band) in the RAMAN spectrum, and the displacement of the signal at 87.03 ppm (C5) in solid-state 13C-NMR confirmed the amide formation. Films including the CS-GO compound were prepared and characterized by thermogravimetric analysis (TGA), where CS-GO film presented a lighter mass loss (~10% less loosed) than CS due probably to the covalent functionalization with GO, providing film thermal resistance. The CS-GO films synthesized were implanted in Wistar rats' subdermal tissue as a first approximation to the biological response. In vivo tests showed a low inflammatory response, good cicatrization, and advanced resorption at 60 days of implantation, as indicated by histological images. It was evidenced that the covalent union between CS and GO increased biocompatibility and the degradation/resorption capacity, demonstrating tissue regeneration with typical characteristics and tiny remnants of implanted material surrounded by a type III collagen capsule. These results show the potential application of the new synthesized films, including the CS-GO compound, in tissue engineering. |
publishDate |
2020 |
dc.date.submitted.none.fl_str_mv |
2020-07-22 |
dc.date.issued.none.fl_str_mv |
2021-06-12 |
dc.date.accessioned.none.fl_str_mv |
2022-12-19T02:43:01Z |
dc.date.available.none.fl_str_mv |
2022-12-19T02:43:01Z |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
dc.type.spa.spa.fl_str_mv |
Artículo |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Valencia AM, Valencia CH, Zuluaga F, Grande-Tovar CD. Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications. Heliyon. 2021 May 21;7(5):e07058. doi: 10.1016/j.heliyon.2021.e07058. PMID: 34095569; PMCID: PMC8165423. |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12834/1145 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.heliyon.2021.e07058. |
dc.identifier.instname.spa.fl_str_mv |
Universidad del Atlántico |
dc.identifier.reponame.spa.fl_str_mv |
Repositorio Universidad del Atlántico |
identifier_str_mv |
Valencia AM, Valencia CH, Zuluaga F, Grande-Tovar CD. Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications. Heliyon. 2021 May 21;7(5):e07058. doi: 10.1016/j.heliyon.2021.e07058. PMID: 34095569; PMCID: PMC8165423. 10.1016/j.heliyon.2021.e07058. Universidad del Atlántico Repositorio Universidad del Atlántico |
url |
https://hdl.handle.net/20.500.12834/1145 |
dc.language.iso.spa.fl_str_mv |
eng |
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eng |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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Attribution-NonCommercial 4.0 International |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc/4.0/ Attribution-NonCommercial 4.0 International http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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dc.publisher.discipline.spa.fl_str_mv |
Química |
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Sede Norte |
dc.source.spa.fl_str_mv |
Heliyon |
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Universidad del Atlántico |
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Valencia, Ana Maríabe3f2f77-a67c-4e97-9ac6-8af5d40a61f8Valencia, Carlos HumbertoZuluaga, FabioGrande-Tovar, Carlos David2022-12-19T02:43:01Z2022-12-19T02:43:01Z2021-06-122020-07-22Valencia AM, Valencia CH, Zuluaga F, Grande-Tovar CD. Synthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applications. Heliyon. 2021 May 21;7(5):e07058. doi: 10.1016/j.heliyon.2021.e07058. PMID: 34095569; PMCID: PMC8165423.https://hdl.handle.net/20.500.12834/114510.1016/j.heliyon.2021.e07058.Universidad del AtlánticoRepositorio Universidad del AtlánticoGraphene oxide (GO) has recently gained attention as a scaffold reinforcing agent for tissue engineering. Biomechanical and biological properties through a synergistic effect can be strengthened when combined with other materials such as chitosan (CS). For that reason, chitosan was used for Graphene Oxide (GO) functionalization through an amide group whose formation was evident by bands around 1600 cm 1 in the FTIR analysis. Furthermore, bands located at 1348 cm 1 (D band), 1593 cm 1 (G band), and 2416 cm 1 (2D band) in the RAMAN spectrum, and the displacement of the signal at 87.03 ppm (C5) in solid-state 13C-NMR confirmed the amide formation. Films including the CS-GO compound were prepared and characterized by thermogravimetric analysis (TGA), where CS-GO film presented a lighter mass loss (~10% less loosed) than CS due probably to the covalent functionalization with GO, providing film thermal resistance. The CS-GO films synthesized were implanted in Wistar rats' subdermal tissue as a first approximation to the biological response. In vivo tests showed a low inflammatory response, good cicatrization, and advanced resorption at 60 days of implantation, as indicated by histological images. It was evidenced that the covalent union between CS and GO increased biocompatibility and the degradation/resorption capacity, demonstrating tissue regeneration with typical characteristics and tiny remnants of implanted material surrounded by a type III collagen capsule. These results show the potential application of the new synthesized films, including the CS-GO compound, in tissue engineering.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2HeliyonSynthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applicationsSynthesis and fabrication of films including graphene oxide functionalized with chitosan for regenerative medicine applicationsPúblico generalChitosan Graphene oxide Regenerative medicine Tissue engineeringinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaQuímicaSede NorteM.A. Nguyen, G. Camci-unal Unconventional tissue engineering materials in disguise Trends Biotechnol., 1–13 (2019)S. Saber-Samandari, S. 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Surg., 135 (5) (2015), pp. 1489-1498http://purl.org/coar/resource_type/c_2df8fbb1ORIGINAL1-s2.0-S2405844021011610-main.pdf1-s2.0-S2405844021011610-main.pdfapplication/pdf3010942https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1145/1/1-s2.0-S2405844021011610-main.pdfc6c580a17c96a78ad1b7117b2ac29badMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1145/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1145/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/1145oai:repositorio.uniatlantico.edu.co:20.500.12834/11452022-12-18 21:43:02.208DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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 |