Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains
Drug resistance in antifungal therapy, a problem unknown until a few years ago, is increasingly assuming importance especially in immunosuppressed patients and patients receiving chemotherapy and radiotherapy. In the past years, the use of essential oils as an approach to improve the effectiveness o...
- Autores:
-
Donadu, Matthew Gavino
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/831
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/831
- Palabra clave:
- : Candida spp.; innovative antifungals; head neck cancer; azole resistant; Ruta graveolens
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| title |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| spellingShingle |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains : Candida spp.; innovative antifungals; head neck cancer; azole resistant; Ruta graveolens |
| title_short |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| title_full |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| title_fullStr |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| title_full_unstemmed |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| title_sort |
Colombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida Strains |
| dc.creator.fl_str_mv |
Donadu, Matthew Gavino |
| dc.contributor.author.none.fl_str_mv |
Donadu, Matthew Gavino |
| dc.contributor.other.none.fl_str_mv |
Peralta-Ruiz, Yeimmy Usai, Donatella Maggio, Francesca Molina-Hernandez, Junior Bernando Rizzo, Davide Bussu, Francesco Rubino, Salvatore Zanetti, Stefania Paparella, Antonello Chaves-Lopez, Clemencia |
| dc.subject.keywords.spa.fl_str_mv |
: Candida spp.; innovative antifungals; head neck cancer; azole resistant; Ruta graveolens |
| topic |
: Candida spp.; innovative antifungals; head neck cancer; azole resistant; Ruta graveolens |
| description |
Drug resistance in antifungal therapy, a problem unknown until a few years ago, is increasingly assuming importance especially in immunosuppressed patients and patients receiving chemotherapy and radiotherapy. In the past years, the use of essential oils as an approach to improve the effectiveness of antifungal agents and to reduce antifungal resistance levels has been proposed. Our research aimed to evaluate the antifungal activity of Colombian rue, Ruta graveolens, essential oil (REO) against clinical strains of Candida albicans, Candida parapsilopsis, Candida glabrata, and Candida tropicalis. Data obtained showed that C. tropicalis and C. albicans were the most sensitive strains showing minimum inhibitory concentrations (MIC) of 4.1 and 8.2 µg/mL of REO. Time–kill kinetics assay demonstrated that REO showed a fungicidal effect against C. tropicalis and a fungistatic effect against C. albicans. In addition, an amount of 40% of the biofilm formed by C. albicans was eradicated using 8.2 µg/mL of REO after 1 h of exposure. The synergistic effect of REO together with some antifungal compounds was also investigated. Fractional inhibitory concentration index (FICI) showed synergic effects of REO combined with amphotericin B. REO Lead a disruption in the cellular membrane integrity, consequently resulting in increased intracellular leakage of the macromolecules, thus confirming that the plasma membrane is a target of the mode of action of REO against C. albicans and C. tropicalis. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021-05-14 |
| dc.date.submitted.none.fl_str_mv |
2021-02-26 |
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2022-11-15T19:38:04Z |
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2022-11-15T19:38:04Z |
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Artículo |
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publishedVersion |
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https://hdl.handle.net/20.500.12834/831 |
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10.3390/jof7050383 |
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Universidad del Atlántico |
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Repositorio Universidad del Atlántico |
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https://hdl.handle.net/20.500.12834/831 |
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10.3390/jof7050383 Universidad del Atlántico Repositorio Universidad del Atlántico |
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eng |
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eng |
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Ingeniería Agroindustrial |
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Sede Norte |
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Journal Of Fungi |
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Donadu, Matthew Gavino14a828f3-86f4-4606-97f0-2f0958489615Peralta-Ruiz, YeimmyUsai, DonatellaMaggio, FrancescaMolina-Hernandez, Junior BernandoRizzo, DavideBussu, FrancescoRubino, SalvatoreZanetti, StefaniaPaparella, AntonelloChaves-Lopez, Clemencia2022-11-15T19:38:04Z2022-11-15T19:38:04Z2021-05-142021-02-26https://hdl.handle.net/20.500.12834/83110.3390/jof7050383Universidad del AtlánticoRepositorio Universidad del AtlánticoDrug resistance in antifungal therapy, a problem unknown until a few years ago, is increasingly assuming importance especially in immunosuppressed patients and patients receiving chemotherapy and radiotherapy. In the past years, the use of essential oils as an approach to improve the effectiveness of antifungal agents and to reduce antifungal resistance levels has been proposed. Our research aimed to evaluate the antifungal activity of Colombian rue, Ruta graveolens, essential oil (REO) against clinical strains of Candida albicans, Candida parapsilopsis, Candida glabrata, and Candida tropicalis. Data obtained showed that C. tropicalis and C. albicans were the most sensitive strains showing minimum inhibitory concentrations (MIC) of 4.1 and 8.2 µg/mL of REO. Time–kill kinetics assay demonstrated that REO showed a fungicidal effect against C. tropicalis and a fungistatic effect against C. albicans. In addition, an amount of 40% of the biofilm formed by C. albicans was eradicated using 8.2 µg/mL of REO after 1 h of exposure. The synergistic effect of REO together with some antifungal compounds was also investigated. Fractional inhibitory concentration index (FICI) showed synergic effects of REO combined with amphotericin B. REO Lead a disruption in the cellular membrane integrity, consequently resulting in increased intracellular leakage of the macromolecules, thus confirming that the plasma membrane is a target of the mode of action of REO against C. albicans and C. tropicalis.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Journal Of FungiColombian Essential Oil of Ruta graveolens against Nosocomial Antifungal Resistant Candida StrainsPúblico general: Candida spp.; innovative antifungals; head neck cancer; azole resistant; Ruta graveolensinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaIngeniería AgroindustrialSede Norte1. Gajdács, M.; Dóczi, I.; Ábrók, M.; Lázár, A.; Burián, K. 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