Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana

Thevetia peruviana is an ornamental shrub grown-up in many tropical region of the world. This plant produces secondary metabolites with biological properties of interest for the pharmaceutical industry. The objective was to determine the secondary metabolites profile of callus and cell suspension cu...

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Autores:
Mendoza, Dary
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad del Atlántico
Repositorio:
Repositorio Uniatlantico
Idioma:
eng
OAI Identifier:
oai:repositorio.uniatlantico.edu.co:20.500.12834/815
Acceso en línea:
https://hdl.handle.net/20.500.12834/815
Palabra clave:
Thevetia peruviana; in vitro cell cultures; secondary metabolites; dihydroquercetin; HPLC.
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openAccess
License
http://creativecommons.org/licenses/by-nc/4.0/
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network_acronym_str UNIATLANT2
network_name_str Repositorio Uniatlantico
repository_id_str
dc.title.spa.fl_str_mv Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
title Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
spellingShingle Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
Thevetia peruviana; in vitro cell cultures; secondary metabolites; dihydroquercetin; HPLC.
title_short Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
title_full Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
title_fullStr Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
title_full_unstemmed Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
title_sort Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana
dc.creator.fl_str_mv Mendoza, Dary
dc.contributor.author.none.fl_str_mv Mendoza, Dary
dc.contributor.other.none.fl_str_mv Arias, Juan Pablo
Cuaspud, Olmedo
Arias, Mario
dc.subject.keywords.spa.fl_str_mv Thevetia peruviana; in vitro cell cultures; secondary metabolites; dihydroquercetin; HPLC.
topic Thevetia peruviana; in vitro cell cultures; secondary metabolites; dihydroquercetin; HPLC.
description Thevetia peruviana is an ornamental shrub grown-up in many tropical region of the world. This plant produces secondary metabolites with biological properties of interest for the pharmaceutical industry. The objective was to determine the secondary metabolites profile of callus and cell suspension cultures of T. peruviana and compare them with those from explant (fruit pulp). Extracts in 50% aqueous ethanol and ethyl acetate were prepared. The phytochemical analysis was performed using standard chemical tests and thin layer chromatography. In addition, total phenolic and flavonoids compounds (TPC and TFC), total cardiac glycosides (TCG) and total antioxidant activity (TAA) was determined during the cell suspension growth. Phenolic chemical profile was also analyzed by high performance liquid chromatography (HPLC). Common metabolites (alkaloids, amino acids, antioxidants, cardiac glycosides, leucoanthocyanidins, flavonoids, phenols, sugars and triterpenes) were detected in all samples. The maximum production of extracellular TCG, TPC, TFC and TAA in cells suspensions were at 6-12 days; in contrast, intracellular content was relatively constant during the exponential grown phase (0 to 12-days). HPLC analysis detected one compound with retention time at 11.6 min; this compound was tentatively identified as dihydroquercetin, a flavonoid with anti-cancer properties. These results provide evidence on the utility of the in vitro cell cultures of T. peruviana for valuable pharmaceutical compounds production.
publishDate 2018
dc.date.submitted.none.fl_str_mv 2018-12-13
dc.date.issued.none.fl_str_mv 2020-02-13
dc.date.accessioned.none.fl_str_mv 2022-11-15T19:27:09Z
dc.date.available.none.fl_str_mv 2022-11-15T19:27:09Z
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.hasVersion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.spa.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12834/815
dc.identifier.doi.none.fl_str_mv 10.1590/1678-4324-2020180735
dc.identifier.instname.spa.fl_str_mv Universidad del Atlántico
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad del Atlántico
url https://hdl.handle.net/20.500.12834/815
identifier_str_mv 10.1590/1678-4324-2020180735
Universidad del Atlántico
Repositorio Universidad del Atlántico
dc.language.iso.spa.fl_str_mv eng
language eng
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dc.rights.cc.*.fl_str_mv Attribution-NonCommercial 4.0 International
dc.rights.accessRights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.place.spa.fl_str_mv Barranquilla
dc.publisher.discipline.spa.fl_str_mv Química
dc.publisher.sede.spa.fl_str_mv Sede Norte
dc.source.spa.fl_str_mv Brazilian Archives of Biology and Technology.
institution Universidad del Atlántico
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spelling Mendoza, Darya54e1cf7-9c6e-4762-b091-1be2d5a6a073Arias, Juan PabloCuaspud, OlmedoArias, Mario2022-11-15T19:27:09Z2022-11-15T19:27:09Z2020-02-132018-12-13https://hdl.handle.net/20.500.12834/81510.1590/1678-4324-2020180735Universidad del AtlánticoRepositorio Universidad del AtlánticoThevetia peruviana is an ornamental shrub grown-up in many tropical region of the world. This plant produces secondary metabolites with biological properties of interest for the pharmaceutical industry. The objective was to determine the secondary metabolites profile of callus and cell suspension cultures of T. peruviana and compare them with those from explant (fruit pulp). Extracts in 50% aqueous ethanol and ethyl acetate were prepared. The phytochemical analysis was performed using standard chemical tests and thin layer chromatography. In addition, total phenolic and flavonoids compounds (TPC and TFC), total cardiac glycosides (TCG) and total antioxidant activity (TAA) was determined during the cell suspension growth. Phenolic chemical profile was also analyzed by high performance liquid chromatography (HPLC). Common metabolites (alkaloids, amino acids, antioxidants, cardiac glycosides, leucoanthocyanidins, flavonoids, phenols, sugars and triterpenes) were detected in all samples. The maximum production of extracellular TCG, TPC, TFC and TAA in cells suspensions were at 6-12 days; in contrast, intracellular content was relatively constant during the exponential grown phase (0 to 12-days). HPLC analysis detected one compound with retention time at 11.6 min; this compound was tentatively identified as dihydroquercetin, a flavonoid with anti-cancer properties. These results provide evidence on the utility of the in vitro cell cultures of T. peruviana for valuable pharmaceutical compounds production.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Brazilian Archives of Biology and Technology.Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruvianaPúblico generalThevetia peruviana; in vitro cell cultures; secondary metabolites; dihydroquercetin; HPLC.info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaQuímicaSede Norte1. Bandara V, Weinstein SA, White J, Eddleston M. A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning. Toxicon. 2010 Sep;56(3):273-81. doi: 10.1016/j.toxicon.2010.03.026.2. Kohls S, Scholz B, Teske J, Zark P, Rullkötter J. Cardiac glycosides from yellow oleander (Thevetia peruviana) seed. Phytochemistry. 2012 Mar;75:114-27. doi: 10.1016/j.phytochem.2011.11.0193. Kohls S, Scholz-Böttcher BM, Teste J, Rullkötter J, Isolation and quantification of six cardiac glycosides from the seeds of Thevetia peruviana provide a basis for toxicological survey. Indian J Biochem Biophys. 2015 Dec;54:1502-10.4. Kumar P, Atreya A, Tanuj T. Thevetia peruviana. Wilderness Environ Med. 2015;26:590-1.5. Kramer M. Pharmacology and therapeutic use of cardiac glycoside thevetin. Arztl Wochensch. 1955 Feb;10(6):1316.6. Hassan MM, Saha AK, Khan SA, Islam A, Mahabub-Uz-Zaman M, Ahmed SSU. Studies on the antidiarrhoeal, antimicrobial and cytotoxic activities of ethanol-extracted leaves of yellow oleander (Thevetia peruviana). Open Vet J. 2011;1(1):28–31.7. 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Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera. Sci Rep. 2016 Oct;6:34464. doi: 10.1038/srep34464.38. Sahin G, Verma SK, Gurel E. Calcium and magnesium elimination enhances accumulation of cardenolides in callus cultures of endemic Digitalis species of Turkey. Plant Physiol Biochem. 2013 Dec;73:139-43. doi: 10.1016/j.plaphy.2013.09.007.39. Kreis W, Reinhard E. Selective Uptake and Vacuolar Storage of Primary Cardiac Glycosides by Suspensioncultured Digitalis lanata Cells. J Plant Physiol. 1987 Jun; 128(4-5):311-26. doi: 10.1016/S0176-1617(87)80117-7.40. Tabata M, Umetani Y, Ooya M, Tanaka S. Glucosylation of phenolic compounds by plant cell cultures. Phytochemistry.1988;27(3):809-13. doi: 10.1016/0031-9422(88)84097-4.41. Voigtländer HW, Balsam G. Apigenin-5-methylether a new flavone from Thevetia peruviana. Arch Pharm Ber Dtsch Pharm Ges. 1970;303(10):7827.42. Abe F, Iwase Y, Yamauchi T, Yaharaj A, Nohara T. Flavonol sinapoyl glycosides from leaves of Thevetia peruviana. Phytochemistry. 1995 Sep;40(2):577-81. doi: 10.1016/0031-9422(95)00316-Y.43. Dixit A, Singh H, Sharma RA, Sharma A. Estimation of antioxidant and antibacterial activity of crude extracts of Thevetia peruviana (Pers.) K. Schum. Int J Pharm. 2015;7:55-9.44. Tiukavkina NA, Rulenko IA, Kolesnik IuA. Dihydroquercetina new antioxidant and biologically active food additive. Vopr Pitan. 1997;6:12-5.45. Weidmann AE. Dihydroquercetin: More than just an impurity?. Eur J Pharmacol. 2012 Jun;684(1-3):19-26. doi: 10.1016/j.ejphar.2012.03.035.46. Oi N, Chen H, Ok Kim M, Lubet RA, Bode AM, Dong Z. Taxifolin suppresses UV-induced skin carcinogenesis by targeting EGFR and PI3-K. Cancer Prev Res (Phila). 2012 Sep;5(9):1103-14. doi: 10.1158/1940-6207.CAPR-11- 0397.47. Sakushima A, Nishibe S. Taxifolin 3-arabinoside from Trachelospermum jasminoides var. Pubescens. 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BMC Plant Biol. 2019 May;19(1):195. doi: 10.1186/s12870-019-1787-x.http://purl.org/coar/resource_type/c_6501ORIGINALdescarga.pdfdescarga.pdfapplication/pdf395399https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/815/1/descarga.pdfb994d7e3098d06f1a495e0202b04eaecMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/815/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/815/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/815oai:repositorio.uniatlantico.edu.co:20.500.12834/8152022-11-15 14:27:10.739DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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