FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate

Plant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies t...

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Autores:
Mendoza, Dary
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Atlántico
Repositorio:
Repositorio Uniatlantico
Idioma:
eng
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oai:repositorio.uniatlantico.edu.co:20.500.12834/851
Acceso en línea:
https://hdl.handle.net/20.500.12834/851
Palabra clave:
Thevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysis
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dc.title.spa.fl_str_mv FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
title FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
spellingShingle FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
Thevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysis
title_short FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
title_full FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
title_fullStr FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
title_full_unstemmed FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
title_sort FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate
dc.creator.fl_str_mv Mendoza, Dary
dc.contributor.author.none.fl_str_mv Mendoza, Dary
dc.contributor.other.none.fl_str_mv Arias, Juan Pablo
Cuaspud, Olmedo
Ruiz, Orlando
Arias, Mario
dc.subject.keywords.spa.fl_str_mv Thevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysis
topic Thevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysis
description Plant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spectroscopy and Reversed-phase high-performance liquid chromatography (RP-HPLC) combined with Multivariate analysis (MVA) were used to detect significant differences in the PC production in cultures treated with two elicitors. The results suggest that the FT-NIR-MVA is useful for discriminating samples according to the treatment, showed significant influence of the PC signal. RP-HPLC-MVA showed that the elicitor effect occurs at 72 h post-elicitation. Detection of dihydroquercetin (maximum concentration = 12.59 mg/L), a flavonoid with anti-cancer properties, is highlighted. Future studies will be aimed at scaling this culture to increase the productivity of dihydroquercetin.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-08-10
dc.date.submitted.none.fl_str_mv 2020-01-31
dc.date.accessioned.none.fl_str_mv 2022-11-15T19:44:55Z
dc.date.available.none.fl_str_mv 2022-11-15T19:44:55Z
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.hasVersion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.spa.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12834/851
dc.identifier.doi.none.fl_str_mv 10.1016/j.btre.2020.e00519
dc.identifier.instname.spa.fl_str_mv Universidad del Atlántico
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad del Atlántico
url https://hdl.handle.net/20.500.12834/851
identifier_str_mv 10.1016/j.btre.2020.e00519
Universidad del Atlántico
Repositorio Universidad del Atlántico
dc.language.iso.spa.fl_str_mv eng
language eng
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dc.publisher.place.spa.fl_str_mv Barranquilla
dc.publisher.sede.spa.fl_str_mv Sede Norte
dc.source.spa.fl_str_mv Biotechnology Reports
institution Universidad del Atlántico
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spelling Mendoza, Darya54e1cf7-9c6e-4762-b091-1be2d5a6a073Arias, Juan PabloCuaspud, OlmedoRuiz, OrlandoArias, Mario2022-11-15T19:44:55Z2022-11-15T19:44:55Z2020-08-102020-01-31https://hdl.handle.net/20.500.12834/85110.1016/j.btre.2020.e00519Universidad del AtlánticoRepositorio Universidad del AtlánticoPlant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spectroscopy and Reversed-phase high-performance liquid chromatography (RP-HPLC) combined with Multivariate analysis (MVA) were used to detect significant differences in the PC production in cultures treated with two elicitors. The results suggest that the FT-NIR-MVA is useful for discriminating samples according to the treatment, showed significant influence of the PC signal. RP-HPLC-MVA showed that the elicitor effect occurs at 72 h post-elicitation. Detection of dihydroquercetin (maximum concentration = 12.59 mg/L), a flavonoid with anti-cancer properties, is highlighted. Future studies will be aimed at scaling this culture to increase the productivity of dihydroquercetin.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Biotechnology ReportsFT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonatePúblico generalThevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysisinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede Norte[1] S. Ramachandra Rao, G.A. Ravishankar, Plant cell cultures: chemical factories of secondary metabolites, Biotechnol. Adv. 20 (2002) 101–153, doi:http://dx. doi.org/10.1016/S0734-9750(02)00007-1.[2] R.J. Whitaker, G.C. Hobbib, L.A. Steward, Production of secondary metabolites in plant cell cultures, Biogeneration of Aromas,(1986), pp. 347–362, doi:http:// dx.doi.org/10.1021/bk-1986-0317.ch026[3] V. Bandara, S.A. Weinstein, J. White, M. Eddleston, A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning, Toxicon 56 (2010) 273–281, doi:http://dx.doi.org/10.1016/j. toxicon.2010.03.026.[4] S. Kohls, B.M. Scholz-Böttcher, J. Teske, P. Zark, J. Rullkötter, Cardiac glycosides from Yellow Oleander (Thevetia peruviana) seeds, Phytochemistry 75 (2012) 114–127, doi:http://dx.doi.org/10.1016/j.phytochem.2011.11.019.[5] S. Haldar, I. Karmakar, M. Chakraborty, D. Ahmad, P.K. Haldar, Antitumor potential of Thevetia peruviana on Ehrlich’s ascites carcinoma-bearing mice, J. Environ. Pathol. Toxicol. Oncol. 34 (2015) 105–113, doi:http://dx.doi.org/ 10.1615/jenvironpatholtoxicoloncol.2015012017.[6] A. 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