Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan
Acrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effe...
- Autores:
-
Valencia Zapata, Mayra Eliana
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/920
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/920
- Palabra clave:
- acrylic bone cement; antibacterial activity; biocompatibility; cell viability; chitosan; graphene oxide; nanocomposite; osseointegration
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| title |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| spellingShingle |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan acrylic bone cement; antibacterial activity; biocompatibility; cell viability; chitosan; graphene oxide; nanocomposite; osseointegration |
| title_short |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| title_full |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| title_fullStr |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| title_full_unstemmed |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| title_sort |
Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan |
| dc.creator.fl_str_mv |
Valencia Zapata, Mayra Eliana |
| dc.contributor.author.none.fl_str_mv |
Valencia Zapata, Mayra Eliana |
| dc.contributor.other.none.fl_str_mv |
Mina Hernandez, José Herminsul Valencia Llano, Carlos Humberto Grande Tovar, Carlos David Vázquez-Lasa, Blanca San Román, Julio Rojo, Luis |
| dc.subject.keywords.spa.fl_str_mv |
acrylic bone cement; antibacterial activity; biocompatibility; cell viability; chitosan; graphene oxide; nanocomposite; osseointegration |
| topic |
acrylic bone cement; antibacterial activity; biocompatibility; cell viability; chitosan; graphene oxide; nanocomposite; osseointegration |
| description |
Acrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effect of introducing graphene oxide (GO) and chitosan (CS), separately or together, in the ABC formulation on setting performance, mechanical behavior, and biological properties. Introduction of both CS and GO to the ABC decreased the maximum temperature by 21% and increased the antibacterial activity against Escherichia coli by 87%, while introduction of only CS decreased bending strength by 32%. The results of cell viability and cell adhesion tests showed in vitro biocompatibility. The in vivo response was investigated using both subdermal and bone parietal implantations in Wistar rats. Modified ABCs showed absence of immune response, as confirmed by a normal inflammatory response in Wistar rat subdermal implantation. The results of the parietal bone implantation showed that the addition of CS and GO together allowed a near total healing bone–cement interface, as observed in the micrographic analysis. The overall results support the great potential of the modified ABCs for application in orthopedic surgery mainly in those cases where osseointegration is required. |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020-09-18 |
| dc.date.submitted.none.fl_str_mv |
2020-07-29 |
| dc.date.accessioned.none.fl_str_mv |
2022-11-15T20:57:48Z |
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2022-11-15T20:57:48Z |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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Artículo |
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publishedVersion |
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https://hdl.handle.net/20.500.12834/920 |
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10.3390/app10186528 |
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Universidad del Atlántico |
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Repositorio Universidad del Atlántico |
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https://hdl.handle.net/20.500.12834/920 |
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10.3390/app10186528 Universidad del Atlántico Repositorio Universidad del Atlántico |
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eng |
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eng |
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Valencia Zapata, Mayra Elianac9423aa9-8912-4a7c-82d7-d8e4335d733eMina Hernandez, José HerminsulValencia Llano, Carlos HumbertoGrande Tovar, Carlos DavidVázquez-Lasa, BlancaSan Román, JulioRojo, Luis2022-11-15T20:57:48Z2022-11-15T20:57:48Z2020-09-182020-07-29https://hdl.handle.net/20.500.12834/92010.3390/app10186528Universidad del AtlánticoRepositorio Universidad del AtlánticoAcrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effect of introducing graphene oxide (GO) and chitosan (CS), separately or together, in the ABC formulation on setting performance, mechanical behavior, and biological properties. Introduction of both CS and GO to the ABC decreased the maximum temperature by 21% and increased the antibacterial activity against Escherichia coli by 87%, while introduction of only CS decreased bending strength by 32%. The results of cell viability and cell adhesion tests showed in vitro biocompatibility. The in vivo response was investigated using both subdermal and bone parietal implantations in Wistar rats. Modified ABCs showed absence of immune response, as confirmed by a normal inflammatory response in Wistar rat subdermal implantation. The results of the parietal bone implantation showed that the addition of CS and GO together allowed a near total healing bone–cement interface, as observed in the micrographic analysis. The overall results support the great potential of the modified ABCs for application in orthopedic surgery mainly in those cases where osseointegration is required.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Applied SciencesOsseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and ChitosanPúblico generalacrylic bone cement; antibacterial activity; biocompatibility; cell viability; chitosan; graphene oxide; nanocomposite; osseointegrationinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaSede Norte1. Slane, J.; Vivanco, J.; Meyer, J.; Ploeg, H.L.; Squire, M. Modification of acrylic bone cement with mesoporous silica nanoparticles: Effects on mechanical, fatigue and absorption properties. J. Mech. Behav. Biomed. Mater. 2014, 29, 451–461.2. Lissarrague, M.H.; Fascio, M.L.; Goyanes, S.; D’Accorso, N.B. Acrylic Bone Cements: The Role of Nanotechnology in Mechanical Properties. J. Biomed. Nanotechnol. 2014, 10, 3536–35573. Franco-Marquès, E.; Méndez, J.A.; Gironès, J.; Ginebra, M.P.; Pèlach, M.A. Evaluation of the influence of the addition of biodegradable polymer matrices in the formulation of self-curing polymer systems for biomedical purposes. Acta Biomater. 2009, 5, 2953–29624. Endogan, T.; Kiziltay, A.; Kose, G.T.; Comunoglu, N.; Beyzadeoglu, T.; Hasirci, N. Acrylic bone cements: Effects of the poly(methyl methacrylate) powder size and chitosan addition on their properties. J. Appl. Polym. Sci. 2014, 131, 396625. Webb, J.C.J.; Spencer, R.F. The role of polymethylmethacrylate bone cement in modern orthopaedic surgery. J. Bone Jt. Surg. 2007, 89, 851–8576. Yan, F.; Liu, Z.; Zhang, T.; Zhang, Q.; Chen, Y.; Xie, Y.; Lei, J.; Cai, L. 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Oral Implantol. 2016, 42, 273–277.http://purl.org/coar/resource_type/c_6501ORIGINALapplsci-10-06528-v2.pdfapplsci-10-06528-v2.pdfapplication/pdf11555111https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/920/1/applsci-10-06528-v2.pdf43145915f6c3509ccbd0258b8d470c1cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/920/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/920/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/920oai:repositorio.uniatlantico.edu.co:20.500.12834/9202022-11-15 15:57:48.972DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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 |