Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi
Background & objectives: Trypanosoma cruzi, the causative agent of American trypanosomiasis, has been reported in 180 mammalian species and 154 triatomines species of Neotropic. This is a clonal parasite with variable biological behaviour, associated with the genetics of the parasite and its hos...
- Autores:
-
Lozano-Arias, Daisy
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad del Atlántico
- Repositorio:
- Repositorio Uniatlantico
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniatlantico.edu.co:20.500.12834/1140
- Acceso en línea:
- https://hdl.handle.net/20.500.12834/1140
- Palabra clave:
- Trypanosoma cruzi; infection kinetics; genotype; heterogeneity; hosts
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/4.0/
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| dc.title.spa.fl_str_mv |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| dc.title.alternative.spa.fl_str_mv |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| title |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| spellingShingle |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi Trypanosoma cruzi; infection kinetics; genotype; heterogeneity; hosts |
| title_short |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| title_full |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| title_fullStr |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| title_full_unstemmed |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| title_sort |
Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi |
| dc.creator.fl_str_mv |
Lozano-Arias, Daisy |
| dc.contributor.author.none.fl_str_mv |
Lozano-Arias, Daisy |
| dc.contributor.other.none.fl_str_mv |
García-Alzate, Roberto Tineo, Evelyn Viettri, Mercedes Mendoza-León, Alexis M. Aguilar, Cruz Morocoima, Antonio Ferrer, Elizabeth Herrera, Leidi |
| dc.subject.keywords.spa.fl_str_mv |
Trypanosoma cruzi; infection kinetics; genotype; heterogeneity; hosts |
| topic |
Trypanosoma cruzi; infection kinetics; genotype; heterogeneity; hosts |
| description |
Background & objectives: Trypanosoma cruzi, the causative agent of American trypanosomiasis, has been reported in 180 mammalian species and 154 triatomines species of Neotropic. This is a clonal parasite with variable biological behaviour, associated with the genetics of the parasite and its hosts. To know the eco-pathogenic complex of this zoonosis, it was proposed to characterize T. cruzi isolates obtained from triatomines and domestic, peridomestic and wild mammals of the eastern and central-western regions of Venezuela. Methods: The positivity to T. cruzi was established and the isolates were genetically characterized by PCR amplification of the mini-exon gene, the DNA coding for 24Sα and 18S rRNA, and polymorphic sequences-RFLPs. The sampling sites were georeferenced using the MapSource Software and ArcGis 9.3 programs to generate distribution maps of the isolates. Results: Of the 460 hosts (205 triatomines and 255 mammals), 49% were positive for the parasite. On the other hand, 38 isolates obtained from the triatomines and 23 isolates obtained from mammals were evaluated. The TcI genotype predominated in most of the isolates; however, in those obtained from triatomines the presence of the TcIII genotype in single infections and TcI + TcIII or TcI + TcIV in mixed infections was also evidenced. Interpretation & conclusion: There is a possibility that the triatomines act as biological syringes for these genotypes associated exclusively to them. The heterogeneity in T. cruzi isolates demonstrated the complexity of parasitosis in these regions, presenting its control and prevention as a challenge. |
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2019 |
| dc.date.issued.none.fl_str_mv |
2019-08-21 |
| dc.date.submitted.none.fl_str_mv |
2020-07-01 |
| dc.date.accessioned.none.fl_str_mv |
2022-12-19T02:42:03Z |
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2022-12-19T02:42:03Z |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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Artículo |
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publishedVersion |
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Lozano-Arias D, García-Alzate R, Tineo E, Viettri M, Mendoza-León A, Aguilar CM, Morocoima A, Ferrer E, Herrera L. Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi. J Vector Borne Dis. 2021 Jan-Mar;58(1):18-27. doi: 10.4103/0972-9062.321749. PMID: 34818859. |
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https://hdl.handle.net/20.500.12834/1140 |
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10.4103/0972-9062.321749. |
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Universidad del Atlántico |
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Repositorio Universidad del Atlántico |
| identifier_str_mv |
Lozano-Arias D, García-Alzate R, Tineo E, Viettri M, Mendoza-León A, Aguilar CM, Morocoima A, Ferrer E, Herrera L. Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi. J Vector Borne Dis. 2021 Jan-Mar;58(1):18-27. doi: 10.4103/0972-9062.321749. PMID: 34818859. 10.4103/0972-9062.321749. Universidad del Atlántico Repositorio Universidad del Atlántico |
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https://hdl.handle.net/20.500.12834/1140 |
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eng |
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eng |
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Attribution-NonCommercial 4.0 International |
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Biología |
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Sede Norte |
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Journal of Vector Borne Diseases |
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Universidad del Atlántico |
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Lozano-Arias, Daisy7e1caede-23ec-4041-b132-6738095ac8bcGarcía-Alzate, RobertoTineo, EvelynViettri, MercedesMendoza-León, AlexisM. Aguilar, CruzMorocoima, AntonioFerrer, ElizabethHerrera, Leidi2022-12-19T02:42:03Z2022-12-19T02:42:03Z2019-08-212020-07-01Lozano-Arias D, García-Alzate R, Tineo E, Viettri M, Mendoza-León A, Aguilar CM, Morocoima A, Ferrer E, Herrera L. Ecopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruzi. J Vector Borne Dis. 2021 Jan-Mar;58(1):18-27. doi: 10.4103/0972-9062.321749. PMID: 34818859.https://hdl.handle.net/20.500.12834/114010.4103/0972-9062.321749.Universidad del AtlánticoRepositorio Universidad del AtlánticoBackground & objectives: Trypanosoma cruzi, the causative agent of American trypanosomiasis, has been reported in 180 mammalian species and 154 triatomines species of Neotropic. This is a clonal parasite with variable biological behaviour, associated with the genetics of the parasite and its hosts. To know the eco-pathogenic complex of this zoonosis, it was proposed to characterize T. cruzi isolates obtained from triatomines and domestic, peridomestic and wild mammals of the eastern and central-western regions of Venezuela. Methods: The positivity to T. cruzi was established and the isolates were genetically characterized by PCR amplification of the mini-exon gene, the DNA coding for 24Sα and 18S rRNA, and polymorphic sequences-RFLPs. The sampling sites were georeferenced using the MapSource Software and ArcGis 9.3 programs to generate distribution maps of the isolates. Results: Of the 460 hosts (205 triatomines and 255 mammals), 49% were positive for the parasite. On the other hand, 38 isolates obtained from the triatomines and 23 isolates obtained from mammals were evaluated. The TcI genotype predominated in most of the isolates; however, in those obtained from triatomines the presence of the TcIII genotype in single infections and TcI + TcIII or TcI + TcIV in mixed infections was also evidenced. Interpretation & conclusion: There is a possibility that the triatomines act as biological syringes for these genotypes associated exclusively to them. The heterogeneity in T. cruzi isolates demonstrated the complexity of parasitosis in these regions, presenting its control and prevention as a challenge.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Journal of Vector Borne DiseasesEcopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruziEcopathogenic complexes of American trypanosomiasis in endemic areas of Venezuela: Diagnosis and variability of Trypanosoma cruziPúblico generalTrypanosoma cruzi; infection kinetics; genotype; heterogeneity; hostsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaBiologíaSede NorteHerrera L. Una revisión sobre reservorios de Trypanosoma (Schizotrypanum) cruzi (Chagas, 1909), agente etiológico de la Enfermedad de Chagas. Bol Mal Salud Amb 2010; 50: 3–15.Zingales B, Miles MA, Campbell DA, Tibayrenc M, Macedo AM, Teixeira MMG, et al. The revised Trypanosoma cruzi subspecific nomenclature: Rationale, epidemiological relevance and research applications. Infect Genet Evol 2012; 12(2): 240– 53World Health Organization 2019. Chagas Disease (American trypanosomiasis). Fact sheet. Available from: https://www.who. int/en/news-room/fact-sheets/detail/chagas-disease (Accessed on April 17, 2019).Colwell DD, Dantas-Torres F, Otranto D. Vector-borne parasitic zoonoses: Emerging scenarios andnew perspectives. Vet Parasitol 2011; 182(1): 14–21.Viettri M, Herrera L, Aguilar CM, Morocoima A, Reyes J, Lares M, et al. Molecular diagnosis of Trypanosoma cruzi/Leishmania spp. coinfection in domestic, peridomestic and wild mammals of Venezuelan co-endemic areas. Vet Parasitol Reg Stud Reports 2018; 14: 123–130.Carrasco H, Segovia M, Llewellyn M, Morocoima A, UrdanetaMorales S, Martínez C, et al. Geographical distribution of Trypanosoma cruzi genotypes in Venezuela. PLoS Negl Trop Dis 2012; 6(6): e1707.Urdaneta-Morales S. Chagas’ disease: an emergent urban zoonosis. The Caracas Valley (Venezuela) as an epidemiological model. Front Public Health 2014; 2(265): 1–13.Morocoima A, Barroeta R, Virguez M, Roschman-González A, Chique JD, Ferrer E et al. Infección natural por Trypanosoma cruzi en triatominos que habitan en la Palma Corozo (Acrocomia aculeata) en regiones del oriente de Venezuela. Rev Peru Med Exp. Salud Pública 2018; 35(4): 563–72.Morocoima A, Tineo E, Ferrer E, Herrera L, Nuñez M. Enfermedad de Chagas en el estado Anzoátegui, Venezuela: Registro de un caso agudo y caracterización parasitológica y molecular del aislado. Bol Malariol Sal Amb 2008; 48 (2): 121–126Morocoima A, Chique J, Zavala-Jaspe R, Díaz-Bello Z, Ferrer E, Urdaneta-Morales S, et al.Commercial coconut palm as an ecotope of Chagas disease vectors in northeastern Venezuela. J Vector Borne Dis 2010; 47(2): 76–84.Ewel J.J, Madriz A, Tosi J. Zonas de vida de Venezuela. Memoria explicativa sobre el mapa ecológico 1976; 2nd edition. Ministerio de Agricultura y Cría, Fondo Nacional de Investigaciones Agropecuarias. Caracas. 265 pp.Non-Probability Sampling. 2009. Available from: http://explorable.com/non-probability-sampling.html (Accessed on June 24, 2013).Morocoima A, Chique J, Herrera L, Urdaneta-Morales S. Eratyrus mucronatus (Stal, 1859) (Hemiptera, Reduviidae, Triatominae): primer registro para el estado Anzoátegui (Venezuela). Bol Malariol Sal Amb 2010; 48(2): 307–310.Bautista NL, García de la Torre GS, De Haro I, Salazar-Shettino PM. 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Model 2006; 190(3-4): 231– 259.Haverkost TR, Gardner SL, Peterson AT. Predicting the distribution of a parasite using the ecological niche model, GARP. Rev Mex Biodivers 2010; 81(3): 895–902.Herrera L. Trypanosoma cruzi, the causal agent of Chagas disease: boundaries between wild and domestic cycles in Venezuela. Front Public Health 2014; 2: 1–4.Díaz-Bello Z, Zavala-Jaspe R, Reyes-Lugo M, Colmenares C, Noya-Alarcón O, Noya O, et al. Urban Trypanosoma cruzi Oral Transmission: from a Zoonotic Founder Focus to the Largest Microepidemic of Chagas Disease. SOJ Microbiol Infect Dis 2016; 4: 1–9.Rivera IM, Moreno EA, González N, Lugo A. Caracterización de aislados de Trypanosoma cruzi del occidente de Venezuela. Rev Ecol Latin Amer 2000; 7: 1–10.Morocoima A, Carrasco HJ, Boadas J, Chique JD, Herrera L, Urdaneta-Morales S. Trypanosoma cruzi III from armadillos (Dasypus novemcinctus novemcinctus) from Northeastern Venezuela and its biological behavior in murine model. 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Bol Mal Salud Amb 2009; 49(2): 251–258.Cazorla-Perfetti DJ, Nieves-Blanco EE. Triatominos de Venezuela: aspectos taxonómicos, biológicos, distribución geográfica e importancia médica. Avances cardiol 2010; 30(4): 347–369.Cruz-Guzmán PJ, Morocoima A, Chique JD, Ramonis-Quintero J, Uzcátegui M, Carrasco H. Psammolestes arthuri naturalmente infectado con Trypanosoma cruzi encontrado en simpatría con Rhodnius prolixus y Triatoma maculata en nidos de aves en el estado Anzoátegui, Venezuela. Saber 2014; 26: 428–440.. Martins K, Andrade C, Barbosa-Silva AN, do Nascimineto GB, Chiari E, Galvao LM et al. Trypanosoma cruzi III causing the indeterminate form of Chagas disease in a semi-arid region of Brazil. International Journal of Infectious Diseases 2015; 39: 68–75.Gaunt MW, Miles MA. The ecotopes and evolution of triatomine bugs (Triatominae) and their associated trypanosomes. Mem Inst Oswaldo Cruz 2000; 95(4): 557–565.Crisante G, Rojas A, Teixeira M, Añez N. 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Proc Biol Sci 2015; 282(1810): 2014–2807.Conde Sangenis LH, Prates Nielebock MA, Carriello da Silva MC, Ribeiro Bento GM. Chagas disease transmission by consumption of game meat: systematic review. Rev Bras Epidemiol 2016; 19(4): 803–811.Castillo-Neyra R, Chou-Chu L, Quispe-Machaca V, AnccaJuarez J, Malaga-Chavez FS, Bastos M. The potential of canine sentinels for reemerging Trypanosoma cruzi transmission. Prev Vet Med 2015; 120(3-4): 349–356.. Gürtler RE, Cardinal MV. Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi. Acta Trop 2015; 151: 32–50.Jansen AM, Xavier S, Roque AL. The multiple and complex and changeable scenarios of the Trypanosoma cruzi transmission cycle in the sylvatic environment. Acta Trop 2015; 151: 1–15.Horwitz P, Wilcox, BA. Parasites, ecosystems and sustainability: an ecological and complex systems perspective. Int J Parasitol 2005; 35(7): 725–32.http://purl.org/coar/resource_type/c_2df8fbb1ORIGINALJVectorBorneDis58118-2297429_062254.pdfJVectorBorneDis58118-2297429_062254.pdfapplication/pdf1680284https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1140/1/JVectorBorneDis58118-2297429_062254.pdf793fdf8c913a21074007416cba50cc4aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1140/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/1140/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/1140oai:repositorio.uniatlantico.edu.co:20.500.12834/11402022-12-18 21:42:04.085DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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 |