A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication
The establishment of Aedes aegypti and the recent invasion of Aedes albopictus has put Colombia at risk, as it now harbours two arboviral vector species. Studies have shown the susceptibility of Aedes aegypti to the vegetative toxins produced by Lysinibacillus sphaericus. This study aims to determin...
- Autores:
-
Dániel Gómez, Mario Felipe
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2020
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/51222
- Acceso en línea:
- http://hdl.handle.net/1992/51222
- Palabra clave:
- Aedes
Escherichia coli
Control biológico de vectores
Lysinibacillus sphaericus
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| title |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| spellingShingle |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication Aedes Escherichia coli Control biológico de vectores Lysinibacillus sphaericus Biología |
| title_short |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| title_full |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| title_fullStr |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| title_full_unstemmed |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| title_sort |
A novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradication |
| dc.creator.fl_str_mv |
Dániel Gómez, Mario Felipe |
| dc.contributor.advisor.none.fl_str_mv |
Dussán Garzón, Jenny |
| dc.contributor.author.none.fl_str_mv |
Dániel Gómez, Mario Felipe |
| dc.subject.armarc.spa.fl_str_mv |
Aedes Escherichia coli Control biológico de vectores Lysinibacillus sphaericus |
| topic |
Aedes Escherichia coli Control biológico de vectores Lysinibacillus sphaericus Biología |
| dc.subject.themes.none.fl_str_mv |
Biología |
| description |
The establishment of Aedes aegypti and the recent invasion of Aedes albopictus has put Colombia at risk, as it now harbours two arboviral vector species. Studies have shown the susceptibility of Aedes aegypti to the vegetative toxins produced by Lysinibacillus sphaericus. This study aims to determine the potential of two L. sphaericus strains in the control of both Aedes species present in Colombia, in both single and mixed species nurseries. Given the similarities between both species, there exists a need to differentiate them for the development of precise control strategies. Accordingly, mixed nurseries employed an mRFP-expressing Escherichia coli strain as a differentiator. Coloration-aided differentiation facilitated the analysis of mixed nursery treatments and provided deeper scrutiny into the variables at play. This showed an increase in the biocontrol efficiency, pointing to probable ecological variables. Bioassays involving Ae. albopictus showed a lower resistance to the one reported for Ae. aegypti in single-species nurseries. The study concludes that the use of E. coli strains expressing fluorescent proteins are useful tools to be employed in areas like public health entomology. Moreover, it was found that the use of L. sphaericus strains for biocontrol of vector mosquitoes is a viable alternative to chemical insecticides. |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2021-08-10T18:16:19Z |
| dc.date.available.none.fl_str_mv |
2021-08-10T18:16:19Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/bachelorThesis |
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http://purl.org/coar/resource_type/c_7a1f |
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Text |
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http://purl.org/redcol/resource_type/TP |
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http://purl.org/coar/resource_type/c_7a1f |
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http://hdl.handle.net/1992/51222 |
| dc.identifier.pdf.none.fl_str_mv |
22732.pdf |
| dc.identifier.instname.spa.fl_str_mv |
instname:Universidad de los Andes |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
| dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repositorio.uniandes.edu.co/ |
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http://hdl.handle.net/1992/51222 |
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22732.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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14 hojas |
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application/pdf |
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Universidad de los Andes |
| dc.publisher.program.none.fl_str_mv |
Biología |
| dc.publisher.faculty.none.fl_str_mv |
Facultad de Ciencias |
| dc.publisher.department.spa.fl_str_mv |
Departamento de Ciencias Biológicas |
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Universidad de los Andes |
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Universidad de los Andes |
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Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87600Dániel Gómez, Mario Felipe12b8dbf2-7389-4b53-96af-90cfdcfc5cd34002021-08-10T18:16:19Z2021-08-10T18:16:19Z2020http://hdl.handle.net/1992/5122222732.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/The establishment of Aedes aegypti and the recent invasion of Aedes albopictus has put Colombia at risk, as it now harbours two arboviral vector species. Studies have shown the susceptibility of Aedes aegypti to the vegetative toxins produced by Lysinibacillus sphaericus. This study aims to determine the potential of two L. sphaericus strains in the control of both Aedes species present in Colombia, in both single and mixed species nurseries. Given the similarities between both species, there exists a need to differentiate them for the development of precise control strategies. Accordingly, mixed nurseries employed an mRFP-expressing Escherichia coli strain as a differentiator. Coloration-aided differentiation facilitated the analysis of mixed nursery treatments and provided deeper scrutiny into the variables at play. This showed an increase in the biocontrol efficiency, pointing to probable ecological variables. Bioassays involving Ae. albopictus showed a lower resistance to the one reported for Ae. aegypti in single-species nurseries. The study concludes that the use of E. coli strains expressing fluorescent proteins are useful tools to be employed in areas like public health entomology. Moreover, it was found that the use of L. sphaericus strains for biocontrol of vector mosquitoes is a viable alternative to chemical insecticides.El establecimiento de Aedes aegypti y la reciente invasión de Aedes albopictus ha puesto a Colombia en riesgo, ya que ahora alberga dos especies de vectores arbovirales. Los estudios han demostrado la susceptibilidad del Aedes aegypti a las toxinas vegetativas producidas por Lysinibacillus sphaericus. Este estudio tiene como objetivo determinar el potencial de dos cepas de L. sphaericus en el control de ambas especies de Aedes presentes en Colombia, tanto en viveros de especies únicas como mixtas. Dadas las similitudes entre ambas especies, existe la necesidad de diferenciarlas para el desarrollo de estrategias de control precisas. En consecuencia, los viveros mixtos emplearon una cepa de Escherichia coli que expresa mRFP como diferenciador. La diferenciación asistida por coloración facilitó el análisis de tratamientos mixtos de vivero y proporcionó un escrutinio más profundo de las variables en juego. Esto mostró un aumento en la eficiencia del biocontrol, apuntando a probables variables ecológicas. Los bioensayos con Ae. albopictus mostró una resistencia menor a la reportada para Ae. aegypti en viveros de una sola especie. El estudio concluye que el uso de cepas de E. coli que expresan proteínas fluorescentes son herramientas útiles para ser empleadas en áreas como la entomología de la salud pública. Además, se encontró que el uso de cepas de L. sphaericus para el control biológico de mosquitos vectores es una alternativa viable a los insecticidas químicos.BiólogoPregrado14 hojasapplication/pdfengUniversidad de los AndesBiologíaFacultad de CienciasDepartamento de Ciencias BiológicasA novel bacterial-mediated method for the differentiation of Aedes spp. larvae in mixed-nursery assays and its application in the determination of vegetative Lysinibacillus sphaericus biocontrol efficiency in invasive vector eradicationTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPAedesEscherichia coliControl biológico de vectoresLysinibacillus sphaericusBiología201423388PublicationTEXT22732.pdf.txt22732.pdf.txtExtracted texttext/plain44828https://repositorio.uniandes.edu.co/bitstreams/5264547e-a8ea-4068-a1e8-22d43f9e5e0b/download149706c34287b162c5c044eb61b4f167MD54THUMBNAIL22732.pdf.jpg22732.pdf.jpgIM Thumbnailimage/jpeg32673https://repositorio.uniandes.edu.co/bitstreams/9541f00e-5a2d-4ef4-a2eb-8aca3de6ac3f/download6f5decb88e5997ab8ab3b409c06bbdf0MD55ORIGINAL22732.pdfapplication/pdf585571https://repositorio.uniandes.edu.co/bitstreams/206ae83c-e1c6-4495-9709-aafb4dd85b56/download8defeae1c910aecdbd4ec8c80d401b6dMD511992/51222oai:repositorio.uniandes.edu.co:1992/512222024-11-14 11:09:39.251http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |