Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia

Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processe...

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Autores:
Numpaque Moreno, Mónica Nohemy
Tipo de recurso:
Fecha de publicación:
2011
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/11611
Acceso en línea:
http://hdl.handle.net/1992/11611
Palabra clave:
Pseudomonas
Combustibles diesel
Explosivos
Minas de carbón
Biología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87600Numpaque Moreno, Mónica Nohemye6ff454b-0557-44d3-9dbe-05722d1c07ce600González Barrios, Andrés FernandoZimmermann, Barbara Hanna2018-09-28T08:05:28Z2018-09-28T08:05:28Z2011http://hdl.handle.net/1992/11611u608616.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processes in coal mining operations. Degradation of the second component of ANFO, which is diesel, by the five isolates and by a consortium was evaluated. Strains were cultured on a minimal salts medium with diesel as the only carbon source. Bacterial growth was monitored during 43 days. The biodegradation of diesel was determined by gas chromatography with a flame ionization detector. All five strains, as well as the consortium, showed a decrease in the total area under the hydrocarbon peaks, suggesting the removal of diesel components. Biodegradation efficiency was 96% for the consortium, individual strains also had high levels of biodegradation efficiency. These results suggest that these bacteria are useful candidates for diesel bioremediation.Magíster en Ciencias BiológicasMaestría27 hojasapplication/pdfengUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Ciencias Biológicasinstname:Universidad de los Andesreponame:Repositorio Institucional SénecaDegradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMPseudomonasCombustibles dieselExplosivosMinas de carbónBiologíaPublicationTHUMBNAILu608616.pdf.jpgu608616.pdf.jpgIM Thumbnailimage/jpeg13215https://repositorio.uniandes.edu.co/bitstreams/ec83ee8b-bf84-4bdc-92a6-64fcb019d9f2/download5d96180cb2d34f5cad4c891c58bab1a1MD55TEXTu608616.pdf.txtu608616.pdf.txtExtracted texttext/plain51143https://repositorio.uniandes.edu.co/bitstreams/61622195-3a32-42cd-9287-6ed870baea05/download88f2f88fda5dad276b997e08bcda0c14MD54ORIGINALu608616.pdfapplication/pdf1014184https://repositorio.uniandes.edu.co/bitstreams/5636eabd-bc70-4ca9-b8d1-906e952e6d9f/downloadc2430b4f598f462174daa5e6f912c560MD511992/11611oai:repositorio.uniandes.edu.co:1992/116112023-10-10 15:30:57.114http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co
dc.title.es_CO.fl_str_mv Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
title Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
spellingShingle Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
Pseudomonas
Combustibles diesel
Explosivos
Minas de carbón
Biología
title_short Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
title_full Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
title_fullStr Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
title_full_unstemmed Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
title_sort Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
dc.creator.fl_str_mv Numpaque Moreno, Mónica Nohemy
dc.contributor.advisor.none.fl_str_mv Dussán Garzón, Jenny
dc.contributor.author.none.fl_str_mv Numpaque Moreno, Mónica Nohemy
dc.contributor.jury.none.fl_str_mv González Barrios, Andrés Fernando
Zimmermann, Barbara Hanna
dc.subject.keyword.es_CO.fl_str_mv Pseudomonas
Combustibles diesel
Explosivos
Minas de carbón
topic Pseudomonas
Combustibles diesel
Explosivos
Minas de carbón
Biología
dc.subject.themes.none.fl_str_mv Biología
description Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processes in coal mining operations. Degradation of the second component of ANFO, which is diesel, by the five isolates and by a consortium was evaluated. Strains were cultured on a minimal salts medium with diesel as the only carbon source. Bacterial growth was monitored during 43 days. The biodegradation of diesel was determined by gas chromatography with a flame ionization detector. All five strains, as well as the consortium, showed a decrease in the total area under the hydrocarbon peaks, suggesting the removal of diesel components. Biodegradation efficiency was 96% for the consortium, individual strains also had high levels of biodegradation efficiency. These results suggest that these bacteria are useful candidates for diesel bioremediation.
publishDate 2011
dc.date.issued.none.fl_str_mv 2011
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dc.date.available.none.fl_str_mv 2018-09-28T08:05:28Z
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dc.identifier.pdf.none.fl_str_mv u608616.pdf
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dc.publisher.program.es_CO.fl_str_mv Maestría en Ciencias Biológicas
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ciencias
dc.publisher.department.es_CO.fl_str_mv Departamento de Ciencias Biológicas
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