Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia
Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processe...
- Autores:
-
Numpaque Moreno, Mónica Nohemy
- Tipo de recurso:
- Fecha de publicación:
- 2011
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/11611
- Acceso en línea:
- http://hdl.handle.net/1992/11611
- Palabra clave:
- Pseudomonas
Combustibles diesel
Explosivos
Minas de carbón
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87600Numpaque Moreno, Mónica Nohemye6ff454b-0557-44d3-9dbe-05722d1c07ce600González Barrios, Andrés FernandoZimmermann, Barbara Hanna2018-09-28T08:05:28Z2018-09-28T08:05:28Z2011http://hdl.handle.net/1992/11611u608616.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processes in coal mining operations. Degradation of the second component of ANFO, which is diesel, by the five isolates and by a consortium was evaluated. Strains were cultured on a minimal salts medium with diesel as the only carbon source. Bacterial growth was monitored during 43 days. The biodegradation of diesel was determined by gas chromatography with a flame ionization detector. All five strains, as well as the consortium, showed a decrease in the total area under the hydrocarbon peaks, suggesting the removal of diesel components. Biodegradation efficiency was 96% for the consortium, individual strains also had high levels of biodegradation efficiency. These results suggest that these bacteria are useful candidates for diesel bioremediation.Magíster en Ciencias BiológicasMaestría27 hojasapplication/pdfengUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Ciencias Biológicasinstname:Universidad de los Andesreponame:Repositorio Institucional SénecaDegradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMPseudomonasCombustibles dieselExplosivosMinas de carbónBiologíaPublicationTHUMBNAILu608616.pdf.jpgu608616.pdf.jpgIM Thumbnailimage/jpeg13215https://repositorio.uniandes.edu.co/bitstreams/ec83ee8b-bf84-4bdc-92a6-64fcb019d9f2/download5d96180cb2d34f5cad4c891c58bab1a1MD55TEXTu608616.pdf.txtu608616.pdf.txtExtracted texttext/plain51143https://repositorio.uniandes.edu.co/bitstreams/61622195-3a32-42cd-9287-6ed870baea05/download88f2f88fda5dad276b997e08bcda0c14MD54ORIGINALu608616.pdfapplication/pdf1014184https://repositorio.uniandes.edu.co/bitstreams/5636eabd-bc70-4ca9-b8d1-906e952e6d9f/downloadc2430b4f598f462174daa5e6f912c560MD511992/11611oai:repositorio.uniandes.edu.co:1992/116112023-10-10 15:30:57.114http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |
dc.title.es_CO.fl_str_mv |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
title |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
spellingShingle |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia Pseudomonas Combustibles diesel Explosivos Minas de carbón Biología |
title_short |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
title_full |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
title_fullStr |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
title_full_unstemmed |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
title_sort |
Degradation of diesel, a component of ANFO, by bacteria selected from an open cast coal mine in La Guajira, Colombia |
dc.creator.fl_str_mv |
Numpaque Moreno, Mónica Nohemy |
dc.contributor.advisor.none.fl_str_mv |
Dussán Garzón, Jenny |
dc.contributor.author.none.fl_str_mv |
Numpaque Moreno, Mónica Nohemy |
dc.contributor.jury.none.fl_str_mv |
González Barrios, Andrés Fernando Zimmermann, Barbara Hanna |
dc.subject.keyword.es_CO.fl_str_mv |
Pseudomonas Combustibles diesel Explosivos Minas de carbón |
topic |
Pseudomonas Combustibles diesel Explosivos Minas de carbón Biología |
dc.subject.themes.none.fl_str_mv |
Biología |
description |
Five bacterial strains were isolated from an open cast coal mine located in La Guajira Colombia. The isolates had previously been characterized and identified as Pseudomonas sp and Pseudomonas stutzeri. The explosive ANFO, composed of ammonium nitrate and diesel fuel, is used for detonation processes in coal mining operations. Degradation of the second component of ANFO, which is diesel, by the five isolates and by a consortium was evaluated. Strains were cultured on a minimal salts medium with diesel as the only carbon source. Bacterial growth was monitored during 43 days. The biodegradation of diesel was determined by gas chromatography with a flame ionization detector. All five strains, as well as the consortium, showed a decrease in the total area under the hydrocarbon peaks, suggesting the removal of diesel components. Biodegradation efficiency was 96% for the consortium, individual strains also had high levels of biodegradation efficiency. These results suggest that these bacteria are useful candidates for diesel bioremediation. |
publishDate |
2011 |
dc.date.issued.none.fl_str_mv |
2011 |
dc.date.accessioned.none.fl_str_mv |
2018-09-28T08:05:28Z |
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2018-09-28T08:05:28Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/masterThesis |
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Text |
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http://purl.org/redcol/resource_type/TM |
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http://hdl.handle.net/1992/11611 |
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u608616.pdf |
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repourl:https://repositorio.uniandes.edu.co/ |
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http://hdl.handle.net/1992/11611 |
identifier_str_mv |
u608616.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
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eng |
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eng |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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27 hojas |
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application/pdf |
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Uniandes |
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Maestría en Ciencias Biológicas |
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Facultad de Ciencias |
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Departamento de Ciencias Biológicas |
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