Guards of the garden: diagnosing diseases in tomato crops in Boyacá and Cundinamarca

This study focuses on identifying and understanding the impact of two major pathogens affecting tomato cultivation in the departments of Boyacá and Cundinamarca. It delves into the correlation between the palo negro symptom and the infection by Pseudomonas syringae, a pathogen responsible for foliar lesions and potential stem necrosis in tomato plants. Additionally, this research investigates the mechanical transmission capabilities of the Tomato Torrado Virus (ToTV) and the complexities of its transmission dynamics. Through the analysis of various tomato samples exhibiting a range of viral symptoms, the presence of ToTV was successfully identified. Utilizing RT-LAMP as a diagnostic tool, the study not only establishes a reliable method for detecting these pathogens but also paves the way for future integration with small RNA (sRNA) sequencing results. The findings of this research are crucial for formulating effective intervention strategies, enhancing disease awareness among tomato farmers, and guiding the implementation of timely control and prevention measures to combat these agricultural challenges.

• Autores: Aparicio Claros, Nicolás
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2024
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: eng
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/73846
• Acceso en línea: https://hdl.handle.net/1992/73846
• Palabra clave: Bacterial infection; Viral infection; Tomato; Bioinformatics; RT-LAMP; Microbiología
• Rights: openAccess
• License: Attribution 4.0 International