Morfología, estructura, composición y dinámica celular de los lipid droplets en levaduras: aproximaciones experimentales hacia su caracterización

The lipid droplets (LDs) are dynamic organelles involved in different cellular processes, mainly in lipid metabolism. They can be biotechnological tools to obtain biofuels, by optimizing the accumulation of lipids within the LDs. They have also been related to mechanisms of pathogenesis of viruses and intracellular bacteria, which is why it is suggested that they may also play an important role in clinically important yeasts. For these reasons, it is important to characterize different properties of LDs that can be related to the metabolic state of the cell at a given moment, such as morphology, abundance, structure, protein and lipid composition, and the flow of lipids towards these organelles. Elucidation of the mechanisms that regulate the biogenesis and the size of the LDs has been attempted through genomic screening and generation of mutants, in order to evaluate the impact of certain genes on the properties listed above. Finally, fluorescence recovery after photobleaching (FRAP) is an increasingly used technique to study the dynamics of proteins present in the monolayer of LDs, since the movement of the protein can be observed in real time. This allows the elucidation of the interactions between the lipid droplets and other organelles. This review will address an overview of the methods of characterization of LDs in yeast.

• Autores: Naranjo Díaz, María Paula
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2020
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: spa
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/51309
• Acceso en línea: http://hdl.handle.net/1992/51309
• Palabra clave: Lípidos; Levaduras; Lipid droplets; Biología molecular; Compuestos orgánicos volátiles; Enzimas; Microbiología
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/4.0/