Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli
When a population of bacteria is exposed to a stress such as an antibiotic, most of the population is killed. However, there is a small fraction of slow growing cells stochastically formed that can survive and become a new population, still susceptible to the antibiotic. This phenomenon is called ba...
- Autores:
-
Contreras González, Lina María
Pérez, María Isabel
Cañas Duarte, Silvia Johana
Restrepo, Silvia
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/34182
- Acceso en línea:
- http://hdl.handle.net/1992/34182
- Palabra clave:
- Escherichia coli - Investigaciones
Resistencia a los medicamentos en microorganismos - Investigaciones - Estudio de casos
Antibióticos - Investigaciones
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.es_CO.fl_str_mv |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| title |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| spellingShingle |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli Escherichia coli - Investigaciones Resistencia a los medicamentos en microorganismos - Investigaciones - Estudio de casos Antibióticos - Investigaciones Biología |
| title_short |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| title_full |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| title_fullStr |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| title_full_unstemmed |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| title_sort |
Effect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coli |
| dc.creator.fl_str_mv |
Contreras González, Lina María Pérez, María Isabel Cañas Duarte, Silvia Johana Restrepo, Silvia |
| dc.contributor.advisor.none.fl_str_mv |
Pedraza Leal, Juan Manuel |
| dc.contributor.author.none.fl_str_mv |
Contreras González, Lina María Pérez, María Isabel Cañas Duarte, Silvia Johana Restrepo, Silvia |
| dc.contributor.jury.none.fl_str_mv |
Reyes, Jinnethe Garavito Aguilar, Zayra Viviana |
| dc.subject.keyword.es_CO.fl_str_mv |
Escherichia coli - Investigaciones Resistencia a los medicamentos en microorganismos - Investigaciones - Estudio de casos Antibióticos - Investigaciones |
| topic |
Escherichia coli - Investigaciones Resistencia a los medicamentos en microorganismos - Investigaciones - Estudio de casos Antibióticos - Investigaciones Biología |
| dc.subject.themes.none.fl_str_mv |
Biología |
| description |
When a population of bacteria is exposed to a stress such as an antibiotic, most of the population is killed. However, there is a small fraction of slow growing cells stochastically formed that can survive and become a new population, still susceptible to the antibiotic. This phenomenon is called bacterial persistence. Given the importance of this type of cells in clinical environments, there is a need to understand their nature and discover the different factors that trigger their formation. In a previous study, using genomics and transcriptomics on the Escherichia coli DS1 (hipQ) strain, a high type II persistence mutant, four mutations in the puuP, ydcK, fadD and cadA genes were identified as candidates for accounting for the hip phenotype. The aim of this study was to study the role of each mutation alone or in combinations with others in a clean TH1268 genetic background. For this, transformed strains were created to study the effect of these mutations... |
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2017 |
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2017 |
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2020-06-10T08:59:15Z |
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2020-06-10T08:59:15Z |
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Trabajo de grado - Maestría |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/masterThesis |
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Text |
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http://purl.org/redcol/resource_type/TM |
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http://hdl.handle.net/1992/34182 |
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u806952.pdf |
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reponame:Repositorio Institucional Séneca |
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repourl:https://repositorio.uniandes.edu.co/ |
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http://hdl.handle.net/1992/34182 |
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u806952.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
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eng |
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eng |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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28 hojas |
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Uniandes |
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Maestría en Ciencias Biológicas |
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Facultad de Ciencias |
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Departamento de Biología |
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Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pedraza Leal, Juan Manuelvirtual::4685-1Contreras González, Lina Maríaab4f4df8-635d-4bd8-b532-d97512b4d4ea600Pérez, María Isabel70c3e499-5beb-47b0-81f2-d3d138a816ae500Cañas Duarte, Silvia Johana49563c6f-083a-47d5-a4fb-22f0681e2333500Restrepo, Silvia73f3639b-ff01-4af7-8720-830435df6e77500Reyes, JinnetheGaravito Aguilar, Zayra Viviana2020-06-10T08:59:15Z2020-06-10T08:59:15Z2017http://hdl.handle.net/1992/34182u806952.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/When a population of bacteria is exposed to a stress such as an antibiotic, most of the population is killed. However, there is a small fraction of slow growing cells stochastically formed that can survive and become a new population, still susceptible to the antibiotic. This phenomenon is called bacterial persistence. Given the importance of this type of cells in clinical environments, there is a need to understand their nature and discover the different factors that trigger their formation. In a previous study, using genomics and transcriptomics on the Escherichia coli DS1 (hipQ) strain, a high type II persistence mutant, four mutations in the puuP, ydcK, fadD and cadA genes were identified as candidates for accounting for the hip phenotype. The aim of this study was to study the role of each mutation alone or in combinations with others in a clean TH1268 genetic background. For this, transformed strains were created to study the effect of these mutations..."Cuando una población de bacterias está expuesta a un estrés como un antibiótico, la mayoría de la población muere. Sin embargo, hay una pequeña fracción de células de crecimiento lento que pueden sobrevivir y convertirse en una nueva población, aún susceptible al antibiótico. Este fenómeno se llama persistencia bacteriana. Dada la importancia de este tipo de células en entornos clínicos, es necesario comprender su naturaleza y descubrir los diferentes factores que desencadenan su formación. En un estudio previo, utilizando genómica y transcriptómica en la cepa Escherichia coli DS1 (hipQ), un mutante de persistencia de tipo II alto, cuatro mutaciones en los genes puuP, ydcK, fadD y cadA se identificaron como candidatos para explicar el fenotipo de la cadera. El objetivo de este estudio fue estudiar el papel de cada mutación sola o en combinación con otras en un fondo genético limpio TH1268. Para esto, se crearon cepas transformadas para estudiar el efecto de estas mutaciones..."--Tomado del Formato de Documento de Grado.Magíster en Ciencias BiológicasMaestría28 hojasapplication/pdfengUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Biologíainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaEffect of mutations in puuP, ydcK, fadD and cadA genes in the generation of persistent cells in Escherichia coliTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMEscherichia coli - InvestigacionesResistencia a los medicamentos en microorganismos - Investigaciones - Estudio de casosAntibióticos - InvestigacionesBiologíaPublicationhttps://scholar.google.es/citations?user=x8-YWMsAAAAJvirtual::4685-10000-0002-1802-3337virtual::4685-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000001817virtual::4685-1a4c0056f-ab75-4234-9297-925380d7633avirtual::4685-1a4c0056f-ab75-4234-9297-925380d7633avirtual::4685-1THUMBNAILu806952.pdf.jpgu806952.pdf.jpgIM Thumbnailimage/jpeg8730https://repositorio.uniandes.edu.co/bitstreams/dc041730-6c15-4bcf-9efd-0ddc9deac71f/download4cffb95b345d2af07af930c37c198160MD55TEXTu806952.pdf.txtu806952.pdf.txtExtracted texttext/plain34995https://repositorio.uniandes.edu.co/bitstreams/30a12d34-5857-41dc-a796-1f6531d9790e/downloadea11a35911bb366934c6359679b5b147MD54ORIGINALu806952.pdfapplication/pdf374592https://repositorio.uniandes.edu.co/bitstreams/74c55385-9905-4df2-97bc-c631900451a0/downloade76de419a4d5aad3892544ba68f8f113MD511992/34182oai:repositorio.uniandes.edu.co:1992/341822024-03-13 12:45:01.527http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |