An analysis pipeline for FAIRE-seq data
Sites across eukaryotic genomes, where disturbance or eviction of nucleosomes from chromatin takes effect, are highly correlated with the presence of active regulatory elements. Formaldehyde-assisted isolation of regulatory elements coupled with high-throughput sequencing (FAIRE-seq) has been proven...
- Autores:
-
Urbina Gómez, David Alejandro
- Tipo de recurso:
- Fecha de publicación:
- 2012
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/11890
- Acceso en línea:
- http://hdl.handle.net/1992/11890
- Palabra clave:
- Biología molecular - Investigaciones
Factores de transcripción - Investigaciones
Genómica funcional - Investigaciones
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
| Summary: | Sites across eukaryotic genomes, where disturbance or eviction of nucleosomes from chromatin takes effect, are highly correlated with the presence of active regulatory elements. Formaldehyde-assisted isolation of regulatory elements coupled with high-throughput sequencing (FAIRE-seq) has been proven successful insolating those genomic regions. FAIRE-seq identified regions agree with results obtained using nuclease hypersensitivity or chromatin immunoprecipitation. Furthermore, FAIRE-seq increases the reproducibility of experiments because it is independent on the use of enzymes and antibodies. However, several computational analyses are required for the interpretation on genome-wide results. Also, FAIRE-seq represents challenges related with its signal characteristics. |
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