Evaluación de la interacción de las proteínas NSs de los virus Andes orthohantavirus, Sin Nombre orthohantavirus, Maciel orthohantavirus, Caño Delgadito orthohantavirus y Necocli orthohantavirus con la proteína IRF 3, precursora del interferón B humano

The hantavirus infection in humans cause zoonotic diseases that occur when a person is in contact with body fluids and/or dry feces of infected mice. The Hantaviridae family is present around the world and, even when not all they cause disease in humans, from those that are pathogens, is not known the specific mechanism by which they evade the immune system, even though previous studies have shown that the NS proteins produced by hantaviruses are involved in the inhibition of Interferon Regulatory Factor 3(IRF3), a precursor of Interferon B. Given what has been said, we chose 5 protein sequences of the hantavirus protein NS, 2 from pathogenic viruses, 2 from not pathogenic viruses and 1 from a Colombian virus Necocli orthohantavirus, whose pathogenicity has not been determined. This selection was made to evaluate whether there are differences in the behavior of pathogenic and non-pathogenic viruses, in addition to observing the behavior of the Colombian virus. Following this, using the software I-Tasser we performed the homology folding of these 5 proteins, while the structure of the free form of the IRF3 was selected from Protein Data Bank. Subsequently, for each protein, we did molecular dynamics and with the results of this procedure, we made a docking process between the NS proteins and the IRF3. These results showed a difference between the type of interaction presented among the NS proteins and the precursor, where the pathogenic viruses proteins interacted in the pocket region of the precursor protein, while the non-pathogenic ones interacted outside of it, for the Colombian protein, we had obtained an interaction with IRF3's external part. With these results, it was possible to establish that there is a difference between the interaction of the NS proteins, pathogenic and non-pathogenic, and the IRF3. Additionally, when we evaluate the behavior of the NS protein of the Colombian virus, which is like that of the pathogenic proteins evaluated before.

• Autores: Quiñones Duque, Juana Isabel
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2021
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: spa
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/53915
• Acceso en línea: http://hdl.handle.net/1992/53915
• Palabra clave: Infecciones por hantavirus; Proteínas; Microbiología
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/4.0/