Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico

A medida que avanza la sociedad y esta continúa creciendo se hace cada vez más presente la necesidad de satisfacer los requerimientos alimenticios de la población global. Tradicionalmente esto ha sido posible mediante el uso de programas de mejoramiento, junto con el uso de fertilizantes, pesticidas...

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Autores:
Racedo Pulido, Camilo
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2023
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
spa
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/69282
Acceso en línea:
http://hdl.handle.net/1992/69282
Palabra clave:
ACCD
Bacterias
Biofertilizantes
IAA
Sideróforos
Microbiología
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openAccess
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https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf
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oai_identifier_str oai:repositorio.uniandes.edu.co:1992/69282
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.none.fl_str_mv Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
title Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
spellingShingle Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
ACCD
Bacterias
Biofertilizantes
IAA
Sideróforos
Microbiología
title_short Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
title_full Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
title_fullStr Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
title_full_unstemmed Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
title_sort Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídrico
dc.creator.fl_str_mv Racedo Pulido, Camilo
dc.contributor.advisor.none.fl_str_mv Bernal Giraldo, Adriana Jimena
Barrera Garzón, Claudia Camila
dc.contributor.author.none.fl_str_mv Racedo Pulido, Camilo
dc.contributor.researchgroup.es_CO.fl_str_mv LIMMA
dc.subject.keyword.none.fl_str_mv ACCD
Bacterias
Biofertilizantes
IAA
Sideróforos
topic ACCD
Bacterias
Biofertilizantes
IAA
Sideróforos
Microbiología
dc.subject.themes.es_CO.fl_str_mv Microbiología
description A medida que avanza la sociedad y esta continúa creciendo se hace cada vez más presente la necesidad de satisfacer los requerimientos alimenticios de la población global. Tradicionalmente esto ha sido posible mediante el uso de programas de mejoramiento, junto con el uso de fertilizantes, pesticidas y otros agroquímicos, sin embargo, este acercamiento genera efectos adversos en el medio ambiente como lixiviación, contaminación, eutrofización, etc. Por esta razón, una alternativa que se ha evaluado para favorecer el crecimiento y producción de sistemas agrícolas se basa en el uso de bacterias promotoras del crecimiento vegetal (PGPB), las cuales funcionan como biofertilizantes, fitoestimuladores, biocontroladores o biorremediadores, siendo una alternativa sostenible para aumentar la producción agrícola. Para este proyecto de tesis se evaluó el potencial de 184 bacterias pertenecientes a la colección del Laboratorio de Interacciones Moleculares de Microorganismos en Agricultura (LIMMA) para promover crecimiento vegetal y aliviar estrés hídrico por medio de pruebas bioquímicas. Para esto, se analizó la capacidad de las bacterias para producir ácido indol-3-ácetico (IAA), producir sideróforos, solubilizar fosfato, crecer en medio libre de nitrógeno, producir ácido 1-aminociclopropano-1-carboxílico desaminasa (ACCD) y producir exopolisacáridos. Adicionalmente se hizo una amplificación del gen nifH para las bacterias que presentaban crecimiento en medio libre de N y se secuenció el gen 16S para las bacterias con una mayor cantidad de pruebas positivas. Finalmente, se realizaron ensayos in vitro utilizando Arabidopsis thaliana como un primer acercamiento a promoción de crecimiento en modelos vegetales. Se encontró que la mayoría de las bacterias aisladas producían IAA, sideróforos y solubilizaban fosfatos, mientras que la producción de ACCD y fijación de nitrógeno fueron las características encontradas en menor proporción. Además, en los ensayos realizados en Arabidopsis se logró encontrar una bacteria que promovía crecimiento por lo cual debería ser considerada para estudios futuros como una PGPB. Sin embargo, se deben realizar ensayos en ambientes con condiciones más reales y con diferentes plantas para caracterizar la capacidad de esta bacteria como PGPB.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-08-04T20:49:22Z
dc.date.available.none.fl_str_mv 2023-08-04T20:49:22Z
dc.date.issued.none.fl_str_mv 2023-08-03
dc.type.es_CO.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.version.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.content.es_CO.fl_str_mv Text
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/TP
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/69282
dc.identifier.instname.es_CO.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.es_CO.fl_str_mv reponame:Repositorio Institucional Séneca
dc.identifier.repourl.es_CO.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
url http://hdl.handle.net/1992/69282
identifier_str_mv instname:Universidad de los Andes
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dc.language.iso.es_CO.fl_str_mv spa
language spa
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spelling Bernal Giraldo, Adriana Jimena721a9490-6cc8-4365-b58a-154fb21deb4a600Barrera Garzón, Claudia Camilab76b9ecc-07ab-45d6-a9db-3e8191843a03600Racedo Pulido, Camilo74b30f32-fb8d-4214-9d43-967c1e4ce14b600LIMMA2023-08-04T20:49:22Z2023-08-04T20:49:22Z2023-08-03http://hdl.handle.net/1992/69282instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/A medida que avanza la sociedad y esta continúa creciendo se hace cada vez más presente la necesidad de satisfacer los requerimientos alimenticios de la población global. Tradicionalmente esto ha sido posible mediante el uso de programas de mejoramiento, junto con el uso de fertilizantes, pesticidas y otros agroquímicos, sin embargo, este acercamiento genera efectos adversos en el medio ambiente como lixiviación, contaminación, eutrofización, etc. Por esta razón, una alternativa que se ha evaluado para favorecer el crecimiento y producción de sistemas agrícolas se basa en el uso de bacterias promotoras del crecimiento vegetal (PGPB), las cuales funcionan como biofertilizantes, fitoestimuladores, biocontroladores o biorremediadores, siendo una alternativa sostenible para aumentar la producción agrícola. Para este proyecto de tesis se evaluó el potencial de 184 bacterias pertenecientes a la colección del Laboratorio de Interacciones Moleculares de Microorganismos en Agricultura (LIMMA) para promover crecimiento vegetal y aliviar estrés hídrico por medio de pruebas bioquímicas. Para esto, se analizó la capacidad de las bacterias para producir ácido indol-3-ácetico (IAA), producir sideróforos, solubilizar fosfato, crecer en medio libre de nitrógeno, producir ácido 1-aminociclopropano-1-carboxílico desaminasa (ACCD) y producir exopolisacáridos. Adicionalmente se hizo una amplificación del gen nifH para las bacterias que presentaban crecimiento en medio libre de N y se secuenció el gen 16S para las bacterias con una mayor cantidad de pruebas positivas. Finalmente, se realizaron ensayos in vitro utilizando Arabidopsis thaliana como un primer acercamiento a promoción de crecimiento en modelos vegetales. Se encontró que la mayoría de las bacterias aisladas producían IAA, sideróforos y solubilizaban fosfatos, mientras que la producción de ACCD y fijación de nitrógeno fueron las características encontradas en menor proporción. Además, en los ensayos realizados en Arabidopsis se logró encontrar una bacteria que promovía crecimiento por lo cual debería ser considerada para estudios futuros como una PGPB. Sin embargo, se deben realizar ensayos en ambientes con condiciones más reales y con diferentes plantas para caracterizar la capacidad de esta bacteria como PGPB.As society advances and continues growing, the need for global food safety is becoming more and more evident. Traditionally, this has been achieved by the implementations of crop improvement programs with concomitant use of fertilizers, pesticides, and other agrochemicals, but the use of these products generates negative effects on the environment such as lixiviation, pollution, eutrophication, etc. For this reason, an alternative that has been evaluated to enhance plant growth and yield is the use of plant growth promoting bacteria (PGPB), which have been shown to function as biofertilizers, phytostimulants, biocontrollers or biorremediators, as an alternative for increasing agricultural yield. For this thesis project the ability as plant growth promoters and the capacity to alleviate water stress was tested for 184 bacteria belonging to the collection of the Laboratorio de Interacciones Moleculares de Microorganismos en Agricultura (LIMMA) using biochemical tests. The capability to produce indole 3 acetic acid (IAA) was evaluated, as well as siderophores production, phosphate solubilization, growth on nitrogen free medium, 1- aminocyclopropane-1-carboxylate deaminase production and exopolysaccharides production. Additionally, for bacteria which grew on N free medium the nifH gen was amplified and the bacteria with the highest amount of positive test the 16S gen was sequenced. Finally, in vitro tests were performed using Arabidopsis thaliana as a first approach for testing growth promotion in plant models. The results showed that most of the bacteria analyzed could produce IAA, siderophores and solubilize phosphate; meanwhile, nitrogen fixation and the production of ACCD were found in lower proportions. Additionally, the Arabidopsis assays showed that one bacteria had the capacity to promote growth at least in this plant model and should be studied more as a PGPB. Nevertheless, it is important to do more tests implementing real growth conditions and with different plant models to further evaluate the capacity of this bacteria as a PGPB.MicrobiólogoPregradoPGPBBiofertilizantesCultivos52 páginasapplication/pdfspaUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias Biológicashttps://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Caracterización de bacterias aisladas de la rizosfera de sacha inchi (Plukenetia volubilis) asociadas a la promoción de crecimiento vegetal y protección contra estrés hídricoTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPACCDBacteriasBiofertilizantesIAASideróforosMicrobiologíaAcevedo, E., Galindo-Castañeda, T., Prada, F., Navia, M. & Romero, H. M. (2014). 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