Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability
Single-cell measurements have appeared in the need to obtain real time viability information with a high resolution. The following thesis aims to design and fabricate a lab-on-a-chip device that allows the detection of adherent cells viability via electrical impedance sprectroscopy (EIS). The device...
- Autores:
-
López Barbosa, Natalia
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2017
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/39739
- Acceso en línea:
- http://hdl.handle.net/1992/39739
- Palabra clave:
- Espectroscopia de impedancia
Cultivo de células
Células vero
Ingeniería
- Rights
- openAccess
- License
- https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf
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Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Osma Cruz, Johann Faccelovirtual::10019-1López Barbosa, Natalia3b44614a-567d-4213-aa08-f217680498fc500Salazar Gómez, Antonio José2020-06-10T16:29:40Z2020-06-10T16:29:40Z2017http://hdl.handle.net/1992/39739u807122.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Single-cell measurements have appeared in the need to obtain real time viability information with a high resolution. The following thesis aims to design and fabricate a lab-on-a-chip device that allows the detection of adherent cells viability via electrical impedance sprectroscopy (EIS). The device is tested with Vero cells at different culture times and compared with traditional MTT viability assay. Two equivalent circuits have been found to describe the behavior of the device with and without the cell culture. The studied device gives and insight of the possibilities of electrochemical measurements in single-cell experiments"Las mediciones de una sola célula han aparecido como consecuencia de la necesidad de obtener información de viabilidad celular en tiempo real y con una alta resolución. La siguiente tesis busca diseñar y fabricar un dispositivo de tipo lab-on-a-chip que permita la detección de la viabilidad celular de líneas adherentes a través de la medición de espectroscopía de impedancias (EIS por sus siglas en inglés). El dispositivo fue probado con células Vero a diferentes tiempos de cultivo y comparado con un ensayo tradicional de viabilidad de MTT. Dos circuitos equivalentes fueron encontrados para describir el comportamiento del dispositivo con y sin el cultivo celular. El dispositivo permite observar las posibilidades de utilizar mediciones electroquímicas en experimentos de una única célula."--Tomado del Formato de Documento de GradoIngeniero ElectrónicoPregrado38 hojasapplication/pdfengUniandesIngeniería ElectrónicaFacultad de IngenieríaDepartamento de Ingeniería Eléctrica y Electrónicainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaDesign and fabrication of an electrochemical sensor for the measurement of adherent cells' viabilityTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPEspectroscopia de impedanciaCultivo de célulasCélulas veroIngenieríaPublicationhttps://scholar.google.es/citations?user=6QQ-dqMAAAAJvirtual::10019-10000-0003-2928-3406virtual::10019-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000221112virtual::10019-1a9f6ef37-65d7-4484-be71-8f3b4067a8favirtual::10019-1a9f6ef37-65d7-4484-be71-8f3b4067a8favirtual::10019-1TEXTu807122.pdf.txtu807122.pdf.txtExtracted texttext/plain32065https://repositorio.uniandes.edu.co/bitstreams/23b1bc16-88c7-457f-a19c-aa03e34cc990/download6dd4ae6038d8f3b9069c768da1a0a0ceMD54THUMBNAILu807122.pdf.jpgu807122.pdf.jpgIM Thumbnailimage/jpeg11373https://repositorio.uniandes.edu.co/bitstreams/48e33272-cf30-4d3a-8cb4-7d8077ecfd25/download3b9533169c09a4198fd70d819f5c7fecMD55ORIGINALu807122.pdfapplication/pdf752674https://repositorio.uniandes.edu.co/bitstreams/c1f36f8f-eea9-4331-ac0b-b09426b54cd3/download8f7a70948ef27896a92fca5b4f141474MD511992/39739oai:repositorio.uniandes.edu.co:1992/397392024-03-13 14:04:51.581https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfopen.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |
| dc.title.es_CO.fl_str_mv |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| title |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| spellingShingle |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability Espectroscopia de impedancia Cultivo de células Células vero Ingeniería |
| title_short |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| title_full |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| title_fullStr |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| title_full_unstemmed |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| title_sort |
Design and fabrication of an electrochemical sensor for the measurement of adherent cells' viability |
| dc.creator.fl_str_mv |
López Barbosa, Natalia |
| dc.contributor.advisor.none.fl_str_mv |
Osma Cruz, Johann Faccelo |
| dc.contributor.author.none.fl_str_mv |
López Barbosa, Natalia |
| dc.contributor.jury.none.fl_str_mv |
Salazar Gómez, Antonio José |
| dc.subject.keyword.es_CO.fl_str_mv |
Espectroscopia de impedancia Cultivo de células Células vero |
| topic |
Espectroscopia de impedancia Cultivo de células Células vero Ingeniería |
| dc.subject.themes.none.fl_str_mv |
Ingeniería |
| description |
Single-cell measurements have appeared in the need to obtain real time viability information with a high resolution. The following thesis aims to design and fabricate a lab-on-a-chip device that allows the detection of adherent cells viability via electrical impedance sprectroscopy (EIS). The device is tested with Vero cells at different culture times and compared with traditional MTT viability assay. Two equivalent circuits have been found to describe the behavior of the device with and without the cell culture. The studied device gives and insight of the possibilities of electrochemical measurements in single-cell experiments |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
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2020-06-10T16:29:40Z |
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2020-06-10T16:29:40Z |
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Trabajo de grado - Pregrado |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/bachelorThesis |
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http://purl.org/coar/resource_type/c_7a1f |
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Text |
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http://purl.org/redcol/resource_type/TP |
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http://purl.org/coar/resource_type/c_7a1f |
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http://hdl.handle.net/1992/39739 |
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u807122.pdf |
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instname:Universidad de los Andes |
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reponame:Repositorio Institucional Séneca |
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http://hdl.handle.net/1992/39739 |
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u807122.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
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eng |
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eng |
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https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf |
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info:eu-repo/semantics/openAccess |
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openAccess |
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38 hojas |
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Uniandes |
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Ingeniería Electrónica |
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Facultad de Ingeniería |
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Departamento de Ingeniería Eléctrica y Electrónica |
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