Comparative mitogenomic analysis of Rhizoctonia anastomosis groups

The genus Rhizoctonia is comprised of soilborne fungal plant pathogens that can affect many crops, ornamental plants, and forest trees worldwide. Species from this genus are classified into multinucleate and binucleate, and each of these groups are divided into anastomosis groups (AGs). R. solani has one of the largest fungal mitogenomes and it is known that it has gone through different expansion processes over time. However, the diversity of the mitogenomes across the anastomosis groups remains largely unknown. In this study, we attempted to assemble 14 mitogenomes from multinucleate and five from binucleate isolates belonging to different AGs. One multinucleate isolate and all the binucleate isolates resulted in mitogenomes ranging from 120,557 bp to 169,413 bp. These were annotated, and gene order was established for each isolate. We found 14 conserved protein coding genes, 23 tRNA, 3 ribosomal genes and two conserved gene blocks, which have been reported for Rhizoctonia before. Moreover, we observed variability in the number of introns ranging from 10 to 30 and homing endonucleases ranging from 14 to 24. A phylogeny based on the nad2 mitogene, which is conserved in all the fungal mitochondrial genomes and is often used for phylogenetic analyses, was constructed including every isolate. The result obtained from this work shows that nad2 is not specific enough to cluster the anastomosis groups together, particularly the binucleates. The main purpose of this study was to better understand the composition and variability of the mitogenomes of different bi- and multinucleate anastomosis groups of Rhizoctonia, using genetic diversity to study the taxonomy and ecology of this diverse genus.

• Autores: Blanco Casallas, Irene
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2024
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: eng
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/73707
• Acceso en línea: https://hdl.handle.net/1992/73707
• Palabra clave: Fungi; Gene synteny; Genomics; Phylogeny; Mitochondria; Microbiología
• Rights: openAccess
• License: Attribution-NonCommercial-NoDerivatives 4.0 International