Phage therapy - opportunities and challenges for its development in Colombia

"Based on the challenges and opportunities we identified for phage therapy, this research aims to point out and narrow the obstacles and take advantage of the open doors to bring phage therapy to Colombia. In this sense, this work is divided into seven chapters that address a group of these cha...

Full description

Autores:
Holguín Moreno, Angela Victoria
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2016
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/7700
Acceso en línea:
http://hdl.handle.net/1992/7700
Palabra clave:
Bacteriófagos - Uso Terapéutico - Investigaciones
Pseudomonas aeruginosa - Investigaciones
Fagocitos - Investigaciones
Resistencia a drogas en microorganismos - Investigaciones
Biología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.title.es_CO.fl_str_mv Phage therapy - opportunities and challenges for its development in Colombia
title Phage therapy - opportunities and challenges for its development in Colombia
spellingShingle Phage therapy - opportunities and challenges for its development in Colombia
Bacteriófagos - Uso Terapéutico - Investigaciones
Pseudomonas aeruginosa - Investigaciones
Fagocitos - Investigaciones
Resistencia a drogas en microorganismos - Investigaciones
Biología
title_short Phage therapy - opportunities and challenges for its development in Colombia
title_full Phage therapy - opportunities and challenges for its development in Colombia
title_fullStr Phage therapy - opportunities and challenges for its development in Colombia
title_full_unstemmed Phage therapy - opportunities and challenges for its development in Colombia
title_sort Phage therapy - opportunities and challenges for its development in Colombia
dc.creator.fl_str_mv Holguín Moreno, Angela Victoria
dc.contributor.advisor.none.fl_str_mv Clockie, Martha
Vives Flórez, Martha Josefina
Reyes Muñoz, Alejandro
dc.contributor.author.none.fl_str_mv Holguín Moreno, Angela Victoria
dc.subject.keyword.es_CO.fl_str_mv Bacteriófagos - Uso Terapéutico - Investigaciones
Pseudomonas aeruginosa - Investigaciones
Fagocitos - Investigaciones
Resistencia a drogas en microorganismos - Investigaciones
topic Bacteriófagos - Uso Terapéutico - Investigaciones
Pseudomonas aeruginosa - Investigaciones
Fagocitos - Investigaciones
Resistencia a drogas en microorganismos - Investigaciones
Biología
dc.subject.themes.none.fl_str_mv Biología
description "Based on the challenges and opportunities we identified for phage therapy, this research aims to point out and narrow the obstacles and take advantage of the open doors to bring phage therapy to Colombia. In this sense, this work is divided into seven chapters that address a group of these challenges and opportunities using different bacteria as models. The first chapter presents the state of the art at the moment of initiating the thesis. By reviewing the literature, we recognized the historical aspects of phage therapy, what was the actual development in the world, in Latin America and in Colombia. This review process allowed the identification of the main challenges and opportunities for the phage therapy in Colombia. The second chapter summarizes the techniques necessary to work with phages. This knowledge and expertise were acquired using a Pseudomonas syringae pv. actinidiae model. In this case, bacteriophages were used successfully to control in vitro the plant pathogen causing canker in kiwifruit plantations in New Zealand. Techniques such as isolation, characterization, in vitro testing, Transmission Electron Microscopy, transduction assays, bacterial growth kinetics, cross-resistance assays, were learned and applied. The third chapter is about phage selection for phage therapy, where Pseudomonas aeruginosa was used as a model. Phage isolation, phage characterization, in vitro testing, and in vivo testing, were performed. This was the first practical approach to phage therapy in our lab. We learned the importance of establishing criteria for phage selection for therapy and categorize the characterization techniques according to its relevance for phage therapy. Chapters 4 and 6 present the interaction between different bacteria and their phages, looking for a deeper understanding of the long-term potential use of the phage therapy. The natural interaction between a phage and its host allows the system to evolve in such a way that an initially susceptible bacterial population can develop resistance. Reciprocally, the phage population might regain infectivity by overcoming the resistance barrier, coexisting in a given environment. These dynamics has been described as co-evolution [Buckling and Rainey, 2002]..."Introducción
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2018-09-27T16:36:30Z
dc.date.available.none.fl_str_mv 2018-09-27T16:36:30Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
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dc.identifier.pdf.none.fl_str_mv u728927.pdf
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dc.format.extent.es_CO.fl_str_mv 173 hojas
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dc.publisher.program.es_CO.fl_str_mv Doctorado en Ciencias - Biología
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ciencias
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Clockie, Martha742260c5-a60d-4895-9dd7-ae0d3ec9eb5b500Vives Flórez, Martha Josefinavirtual::7242-1Reyes Muñoz, Alejandrovirtual::7243-1Holguín Moreno, Angela Victoriaa9179766-2bc9-421e-946e-3674cebd1a335002018-09-27T16:36:30Z2018-09-27T16:36:30Z2016http://hdl.handle.net/1992/770010.57784/1992/7700u728927.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/"Based on the challenges and opportunities we identified for phage therapy, this research aims to point out and narrow the obstacles and take advantage of the open doors to bring phage therapy to Colombia. In this sense, this work is divided into seven chapters that address a group of these challenges and opportunities using different bacteria as models. The first chapter presents the state of the art at the moment of initiating the thesis. By reviewing the literature, we recognized the historical aspects of phage therapy, what was the actual development in the world, in Latin America and in Colombia. This review process allowed the identification of the main challenges and opportunities for the phage therapy in Colombia. The second chapter summarizes the techniques necessary to work with phages. This knowledge and expertise were acquired using a Pseudomonas syringae pv. actinidiae model. In this case, bacteriophages were used successfully to control in vitro the plant pathogen causing canker in kiwifruit plantations in New Zealand. Techniques such as isolation, characterization, in vitro testing, Transmission Electron Microscopy, transduction assays, bacterial growth kinetics, cross-resistance assays, were learned and applied. The third chapter is about phage selection for phage therapy, where Pseudomonas aeruginosa was used as a model. Phage isolation, phage characterization, in vitro testing, and in vivo testing, were performed. This was the first practical approach to phage therapy in our lab. We learned the importance of establishing criteria for phage selection for therapy and categorize the characterization techniques according to its relevance for phage therapy. Chapters 4 and 6 present the interaction between different bacteria and their phages, looking for a deeper understanding of the long-term potential use of the phage therapy. The natural interaction between a phage and its host allows the system to evolve in such a way that an initially susceptible bacterial population can develop resistance. Reciprocally, the phage population might regain infectivity by overcoming the resistance barrier, coexisting in a given environment. 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