Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment
Most human activities that use water produce wastewater and, as the overall demand for water grows, the quantity of wastewater produced and its overall pollution load are continuously increasing worldwide. If wasted water is not properly treated, it may negatively impact the environment and human he...
- Autores:
-
Sotelo Briceño, Laura Daniela
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/59136
- Acceso en línea:
- http://hdl.handle.net/1992/59136
- Palabra clave:
- Microfluidic systems
Microencapsulado
Microfluídica
Purificación de aguas residuales
Biodegradación de aguas residuales
Microbiología
- Rights
- openAccess
- License
- Attribution-NoDerivatives 4.0 Internacional
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Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| title |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| spellingShingle |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment Microfluidic systems Microencapsulado Microfluídica Purificación de aguas residuales Biodegradación de aguas residuales Microbiología |
| title_short |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| title_full |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| title_fullStr |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| title_full_unstemmed |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| title_sort |
Polymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment |
| dc.creator.fl_str_mv |
Sotelo Briceño, Laura Daniela |
| dc.contributor.advisor.none.fl_str_mv |
Osma Cruz, Johan Faccelo Vives Flórez, Martha Josefina |
| dc.contributor.author.none.fl_str_mv |
Sotelo Briceño, Laura Daniela |
| dc.contributor.researchgroup.es_CO.fl_str_mv |
Biomycrosystems |
| dc.subject.keyword.none.fl_str_mv |
Microfluidic systems Microencapsulado Microfluídica Purificación de aguas residuales Biodegradación de aguas residuales |
| topic |
Microfluidic systems Microencapsulado Microfluídica Purificación de aguas residuales Biodegradación de aguas residuales Microbiología |
| dc.subject.themes.es_CO.fl_str_mv |
Microbiología |
| description |
Most human activities that use water produce wastewater and, as the overall demand for water grows, the quantity of wastewater produced and its overall pollution load are continuously increasing worldwide. If wasted water is not properly treated, it may negatively impact the environment and human health, this is one of the reasons why wastewater treatment has become an important worldwide priority during the past decade. However, conventional wastewater treatment technologies, like activated sludge, cannot eliminate these pollutants completely, so enzymatic treatment arises as an alternative method for the treatment of organic micropollutants. Here, we aimed at microencapsulating Laccase, by producing monodisperse alginatebased microcapsules through a droplet-based microfluidic system, and establish the Laccase encapsulation efficiency and its enzymatic activity in different pH and temperature conditions, in order to determine its potential use as a biocatalyst in wastewater treatment. In this sense, it was first aimed to manufacture a microsystem device and determine the best parameters for microcapsule generation, in order to encapsulate the enzyme and determine its immobilization efficiency. Then, the optimum pH and temperature conditions were determined to perform the preliminary tests of the encapsulated Laccase in the microreactor. It is expected to obtain a high percentage of micropollutants removal from the artificial water samples, and also a low loss of enzyme from the microcapsules over time. |
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2022 |
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2022-07-25T20:23:38Z |
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2022-07-25T20:23:38Z |
| dc.date.issued.none.fl_str_mv |
2022-07-24 |
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Trabajo de grado - Pregrado |
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info:eu-repo/semantics/bachelorThesis |
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http://hdl.handle.net/1992/59136 |
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eng |
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eng |
| dc.relation.references.es_CO.fl_str_mv |
Tilley, E., Compendium of sanitation systems and technologies; Eawag: 2014. Water, U. The United Nations World Water Development Report 2017. Margot, J.; Rossi, L.; Barry, D. A.; Holliger, C. Wiley Interdisciplinary Reviews: Water 2015, 2, 457-487. Paul, P. E. V.; Sangeetha, V.; Deepika, R. G. In Recent developments in applied microbiology and biochemistry; Elsevier: 2019, pp 107-125. Ferreira, D. P.; Cruz, J.; Fangueiro, R. In Green composites for automotive applications; Elsevier: 2019, pp 3-41. Berg, J.; Tymoczko, J.; Stryer, L Biochemistry 2002. Chapman, J.; Ismail, A. E.; Dinu, C. Z. Catalysts 2018, 8, 238. Jegannathan, K. R.; Nielsen, P. H. Journal of cleaner production 2013, 42, 228-240. Barber, E. A.; Liu, Z.; Smith, S. R. Microorganisms 2020, 8, 122. Singh, M.; Hemant, K.; Ram, M; Shivakumar, H. Research in pharmaceutical sciences 2010, 5, 65. Campaña, A. L.; Sotelo, D. C.; Oliva, H. A.; Aranguren, A.; Ornelas-Soto, N.; Cruz, J. C.; Osma, J. F. Polymers 2020, 12, 1158 Niku-Paavola, M.-L.; Raaska, L.; Itävaara, M. Mycological Research 1990, 94, 27-31. Lopez-Barbosa, N.; Campaña, A. L.; Cruz, J. C.; Ornelas-Soto, N.; Osma, J. F. Polymers 2020, 12, 1353. Alberty, R. A.; Massey, V.; Frieden, C.; Fuhlbrigge, A. R. Journal of the American Chemical Society 1954, 76, 2485-2493 Mazlan, S. Z.; Hanifah, S. A. International Journal of Polymer Science 2017, 2017. Cristóvão, R. O.; Silvério, S. C.; Tavares, A. P.; Brígida, A. I. S.; Loureiro, J. M.; Boaventura, R. A.; Macedo, E. A.; Coelho, M. A. Z.World Journal of Microbiology and Biotechnology 2012, 28, 2827-2838 Pirker, K. F.; Baratto, M. C.; Basosi, R.; Goodman, B. A. Journal of inorganic biochemistry 2012, 112, 10-16. Yin, Q.; Zhou, G.; Peng, C.; Zhang, Y.; Kües, U.; Liu, J.; Xiao, Y.; Fang, Z. AMB Express 2019, 9, 1-13. Clayden, J.; Greeves, N.;Warren, S.;Wothers, P. Organic chemistry, 2001. Mujahid, A.; Ali, Y.; Afzal, A.; Hussain, T.; Shah, A. T.; Shehzad, K.; Farooq, M. U. Arabian Journal of Chemistry 2014, 7, 522-524. Manu, B.; Mahamood, S; Vittal, H; Shrihari, S, et al. IJRCE 2011, 1, 157. Hoang, V. D.; Ly, D. T. H.; Tho, N. H.; Minh Thi Nguyen, H. The Scientific World Journal 2014, 2014. Skoog, D. A.; West, D. M.; Holler, F. J.; Crouch, S. R., Fundamentals of analytical chemistry; Cengage learning: 2013. |
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Universidad de los Andes |
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Microbiología |
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Facultad de Ciencias |
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Departamento de Ciencias Biológicas |
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Attribution-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Osma Cruz, Johan Facceloe4467a1a-d4b5-43a8-8639-efbf0020b552600Vives Flórez, Martha Josefina0e8da18b-7a87-4f29-9af5-33050bd372b2400Sotelo Briceño, Laura Daniela1fca889f-7f2b-40bf-9578-955be63aeca2600Biomycrosystems2022-07-25T20:23:38Z2022-07-25T20:23:38Z2022-07-24http://hdl.handle.net/1992/59136instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Most human activities that use water produce wastewater and, as the overall demand for water grows, the quantity of wastewater produced and its overall pollution load are continuously increasing worldwide. If wasted water is not properly treated, it may negatively impact the environment and human health, this is one of the reasons why wastewater treatment has become an important worldwide priority during the past decade. However, conventional wastewater treatment technologies, like activated sludge, cannot eliminate these pollutants completely, so enzymatic treatment arises as an alternative method for the treatment of organic micropollutants. Here, we aimed at microencapsulating Laccase, by producing monodisperse alginatebased microcapsules through a droplet-based microfluidic system, and establish the Laccase encapsulation efficiency and its enzymatic activity in different pH and temperature conditions, in order to determine its potential use as a biocatalyst in wastewater treatment. In this sense, it was first aimed to manufacture a microsystem device and determine the best parameters for microcapsule generation, in order to encapsulate the enzyme and determine its immobilization efficiency. Then, the optimum pH and temperature conditions were determined to perform the preliminary tests of the encapsulated Laccase in the microreactor. It is expected to obtain a high percentage of micropollutants removal from the artificial water samples, and also a low loss of enzyme from the microcapsules over time.MicrobiólogoPregrado30 páginasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasPolymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatmentTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPMicrofluidic systemsMicroencapsuladoMicrofluídicaPurificación de aguas residualesBiodegradación de aguas residualesMicrobiologíaTilley, E., Compendium of sanitation systems and technologies; Eawag: 2014.Water, U. The United Nations World Water Development Report 2017.Margot, J.; Rossi, L.; Barry, D. A.; Holliger, C. Wiley Interdisciplinary Reviews: Water 2015, 2, 457-487.Paul, P. E. V.; Sangeetha, V.; Deepika, R. G. In Recent developments in applied microbiology and biochemistry; Elsevier: 2019, pp 107-125.Ferreira, D. P.; Cruz, J.; Fangueiro, R. In Green composites for automotive applications; Elsevier: 2019, pp 3-41.Berg, J.; Tymoczko, J.; Stryer, L Biochemistry 2002.Chapman, J.; Ismail, A. E.; Dinu, C. Z. Catalysts 2018, 8, 238.Jegannathan, K. R.; Nielsen, P. H. Journal of cleaner production 2013, 42, 228-240.Barber, E. A.; Liu, Z.; Smith, S. R. Microorganisms 2020, 8, 122.Singh, M.; Hemant, K.; Ram, M; Shivakumar, H. Research in pharmaceutical sciences 2010, 5, 65.Campaña, A. L.; Sotelo, D. C.; Oliva, H. A.; Aranguren, A.; Ornelas-Soto, N.; Cruz, J. C.; Osma, J. F. Polymers 2020, 12, 1158Niku-Paavola, M.-L.; Raaska, L.; Itävaara, M. Mycological Research 1990, 94, 27-31.Lopez-Barbosa, N.; Campaña, A. L.; Cruz, J. C.; Ornelas-Soto, N.; Osma, J. F. Polymers 2020, 12, 1353.Alberty, R. A.; Massey, V.; Frieden, C.; Fuhlbrigge, A. R. Journal of the American Chemical Society 1954, 76, 2485-2493Mazlan, S. Z.; Hanifah, S. A. International Journal of Polymer Science 2017, 2017.Cristóvão, R. O.; Silvério, S. C.; Tavares, A. P.; Brígida, A. I. S.; Loureiro, J. M.; Boaventura, R. A.; Macedo, E. A.; Coelho, M. A. Z.World Journal of Microbiology and Biotechnology 2012, 28, 2827-2838Pirker, K. F.; Baratto, M. C.; Basosi, R.; Goodman, B. A. Journal of inorganic biochemistry 2012, 112, 10-16.Yin, Q.; Zhou, G.; Peng, C.; Zhang, Y.; Kües, U.; Liu, J.; Xiao, Y.; Fang, Z. AMB Express 2019, 9, 1-13.Clayden, J.; Greeves, N.;Warren, S.;Wothers, P. Organic chemistry, 2001.Mujahid, A.; Ali, Y.; Afzal, A.; Hussain, T.; Shah, A. T.; Shehzad, K.; Farooq, M. U. Arabian Journal of Chemistry 2014, 7, 522-524.Manu, B.; Mahamood, S; Vittal, H; Shrihari, S, et al. IJRCE 2011, 1, 157.Hoang, V. D.; Ly, D. T. H.; Tho, N. H.; Minh Thi Nguyen, H. The Scientific World Journal 2014, 2014.Skoog, D. A.; West, D. M.; Holler, F. J.; Crouch, S. R., Fundamentals of analytical chemistry; Cengage learning: 2013.201629895PublicationTHUMBNAILPolymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment.pdf.jpgPolymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment.pdf.jpgIM Thumbnailimage/jpeg9355https://repositorio.uniandes.edu.co/bitstreams/e77dfb2f-3cea-4f04-9684-948d3f556107/download15e1726038ce4451cbee1842c685dab1MD57Autorizacio¿n entrega tesis_jfo LDS MV.pdf.jpgAutorizacio¿n entrega tesis_jfo LDS MV.pdf.jpgIM Thumbnailimage/jpeg15871https://repositorio.uniandes.edu.co/bitstreams/a0397cbb-d17b-4f71-a9ca-80a3cae2f214/download8cdfdec36778c2c8f1d5fe40b11aec64MD59ORIGINALPolymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment.pdfPolymeric microencapsulation of the fungal enzyme Laccase, through microfluidic systems, for its potential use as a biocatalyst in wastewater treatment.pdfTrabajo de Gradoapplication/pdf4488519https://repositorio.uniandes.edu.co/bitstreams/4fb6bf05-3f63-407a-9aad-7e4cb92fa67b/download50b36110dfa178cada82d62cfdc1a5cfMD53Autorizacio¿n entrega tesis_jfo LDS MV.pdfAutorizacio¿n entrega tesis_jfo LDS MV.pdfHIDEapplication/pdf294203https://repositorio.uniandes.edu.co/bitstreams/f92671d3-2653-4b20-b4fb-466f250a1235/download19059dbcf1c937bf40dbb0a210d7eea8MD54LICENSElicense.txtlicense.txttext/plain; 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