Selección de un consorcio microbiano lignocelulolítico a partir de poblaciones de bacterias ácido lácticas como estrategia para el mejoramiento de un proceso de ensilaje de forrajes

In this study, a microbial consortium with lignocellulolytic capacity was selected from an inoculum of epiphytic lactic acid bacteria obtained from the biomass of Megathyrsus maximus (forage). The dilution-to-stimulation approach was used for the selection of this consortium. In the enrichment strategy, the MRS culture medium (i.e. Man, Rogosa and Sharpe) was modified throughout the transfers and forage was used as the only source of carbon. Within the process, cell growth was quantified by optical density and colony forming unit (CFU) counts in the MRS (unmodified) and R2A media, and the percentage of substrate degradation. Interestingly, a decrease in both optical density and CFU count throughout the transfers was obtained, showing a greater statistical difference between transfer 1 and 6. Additionally, during transfers 1 and 5, growth was observed in the negative control, which contained no substrate. This indicated that the selected microorganisms were using other carbon sources than forage...

• Autores: Chaparro Avellaneda, Dayanne Paola; Díaz García, Laura Andrea
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2021
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: spa
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/51306
• Acceso en línea: http://hdl.handle.net/1992/51306
• Palabra clave: Ensilaje; Forraje; Bacterias lácticas; Biomasa; Microbiología
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/4.0/