Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access

"Chronic kidney disease (CKD) needs improved therapy options to ensure better quality of life for patients. Since kidneys are not easily available for each patient, renal replacement therapies are still necessary to remove waste from the blood, as a healthy kidney would do. Hemodialysis being o...

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Autores:
Valencia Rivero, Karen Tatiana
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2018
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/38690
Acceso en línea:
http://hdl.handle.net/1992/38690
Palabra clave:
Hemodinámica - Investigaciones
Fístula arteriovenosa - Investigaciones
Hemodiálisis - Investigaciones
Trasplantes de órganos, tejidos, etc - Investigaciones
Medicina regenerativa - Investigaciones - Estudio de casos
Ingeniería de tejidos - Investigaciones - Estudio de casos
Ingeniería
Rights
openAccess
License
https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf
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network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.es_CO.fl_str_mv Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
title Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
spellingShingle Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
Hemodinámica - Investigaciones
Fístula arteriovenosa - Investigaciones
Hemodiálisis - Investigaciones
Trasplantes de órganos, tejidos, etc - Investigaciones
Medicina regenerativa - Investigaciones - Estudio de casos
Ingeniería de tejidos - Investigaciones - Estudio de casos
Ingeniería
title_short Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
title_full Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
title_fullStr Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
title_full_unstemmed Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
title_sort Design, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis access
dc.creator.fl_str_mv Valencia Rivero, Karen Tatiana
dc.contributor.advisor.none.fl_str_mv Briceño Triana, Juan Carlos
Wagner, William R.
dc.contributor.author.none.fl_str_mv Valencia Rivero, Karen Tatiana
dc.contributor.jury.none.fl_str_mv Cruz Jiménez, Juan Carlos
Sandoval Reyes, Néstor Fernando
dc.subject.keyword.es_CO.fl_str_mv Hemodinámica - Investigaciones
Fístula arteriovenosa - Investigaciones
Hemodiálisis - Investigaciones
Trasplantes de órganos, tejidos, etc - Investigaciones
Medicina regenerativa - Investigaciones - Estudio de casos
Ingeniería de tejidos - Investigaciones - Estudio de casos
topic Hemodinámica - Investigaciones
Fístula arteriovenosa - Investigaciones
Hemodiálisis - Investigaciones
Trasplantes de órganos, tejidos, etc - Investigaciones
Medicina regenerativa - Investigaciones - Estudio de casos
Ingeniería de tejidos - Investigaciones - Estudio de casos
Ingeniería
dc.subject.themes.none.fl_str_mv Ingeniería
description "Chronic kidney disease (CKD) needs improved therapy options to ensure better quality of life for patients. Since kidneys are not easily available for each patient, renal replacement therapies are still necessary to remove waste from the blood, as a healthy kidney would do. Hemodialysis being one of the most common long-term treatments for CKD, is in the need of a vascular access, an arteriovenous connection currently made with synthetic materials as expanded polytetrafluoroethylene (ePTFE), that have important complications as intimal hyperplasia, infection and thrombosis, due to the chemical, biological and mechanical properties when compared to native blood vessels of the patients. To face complications of current ePTFE vascular grafts, in this study small intestinal submucosa (SIS) was decellularized and carefully rolled to manufacture flexible C-shaped vascular grafts, four millimeters of diameter, capable to restrain arterial flow without any kink or complication in the arterial and venous anastomosis. Even if patency rates and regeneration were superior in SIS based vascular grafts than ePTFE, thrombosis remained as the main complication during the maturation process. To reduce thrombogenesis, three carboxyl-terminated macromolecules with different molecular weights were conjugated to the pendant amine groups on the SIS surface: zwitterionic phosphorylcholine, carboxylic acids and polyethylene glycol. Reduction in platelet deposition was significant with carboxylic acids, but remarkable with zwitterionic macromolecules of Phosphorylcholine, and even better with Polyethylene Glycol. PEGylation of SIS was easy, cheap, and even with a low conjugation yield, improved the SIS mechanical properties making SIS vascular grafts significantly like native arteries, without altering the biocompatible regenerative properties of the SIS. Results obtained in this thesis show the SIS capability to adapt to different conditions, remaining as an excellent scaffold for different."--Tomado del Formato de Documento de Grado
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2020-06-10T14:27:55Z
dc.date.available.none.fl_str_mv 2020-06-10T14:27:55Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/38690
dc.identifier.doi.none.fl_str_mv 10.57784/1992/38690
dc.identifier.pdf.none.fl_str_mv u807590.pdf
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Séneca
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
url http://hdl.handle.net/1992/38690
identifier_str_mv 10.57784/1992/38690
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dc.publisher.es_CO.fl_str_mv Uniandes
dc.publisher.program.es_CO.fl_str_mv Doctorado en Ingeniería
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ingeniería
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Briceño Triana, Juan Carlos01e2068c-10b1-4267-9d44-a762151a26a9400Wagner, William R.e9a558a7-987a-4c76-b9f6-1111b8455fd9500Valencia Rivero, Karen Tatiana29781500Cruz Jiménez, Juan CarlosSandoval Reyes, Néstor Fernando2020-06-10T14:27:55Z2020-06-10T14:27:55Z2018http://hdl.handle.net/1992/3869010.57784/1992/38690u807590.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/"Chronic kidney disease (CKD) needs improved therapy options to ensure better quality of life for patients. Since kidneys are not easily available for each patient, renal replacement therapies are still necessary to remove waste from the blood, as a healthy kidney would do. Hemodialysis being one of the most common long-term treatments for CKD, is in the need of a vascular access, an arteriovenous connection currently made with synthetic materials as expanded polytetrafluoroethylene (ePTFE), that have important complications as intimal hyperplasia, infection and thrombosis, due to the chemical, biological and mechanical properties when compared to native blood vessels of the patients. To face complications of current ePTFE vascular grafts, in this study small intestinal submucosa (SIS) was decellularized and carefully rolled to manufacture flexible C-shaped vascular grafts, four millimeters of diameter, capable to restrain arterial flow without any kink or complication in the arterial and venous anastomosis. Even if patency rates and regeneration were superior in SIS based vascular grafts than ePTFE, thrombosis remained as the main complication during the maturation process. To reduce thrombogenesis, three carboxyl-terminated macromolecules with different molecular weights were conjugated to the pendant amine groups on the SIS surface: zwitterionic phosphorylcholine, carboxylic acids and polyethylene glycol. Reduction in platelet deposition was significant with carboxylic acids, but remarkable with zwitterionic macromolecules of Phosphorylcholine, and even better with Polyethylene Glycol. PEGylation of SIS was easy, cheap, and even with a low conjugation yield, improved the SIS mechanical properties making SIS vascular grafts significantly like native arteries, without altering the biocompatible regenerative properties of the SIS. Results obtained in this thesis show the SIS capability to adapt to different conditions, remaining as an excellent scaffold for different."--Tomado del Formato de Documento de Grado"La enfermedad renal crónica (ERC) necesita mejores opciones de tratamiento para garantizar una mejor calidad de vida para los pacientes. Dado que los riñones no están disponibles fácilmente para cada paciente, las terapias de reemplazo renal siguen siendo necesarias para eliminar los desechos de la sangre, como lo haría un riñón saludable. La hemodiálisis es uno de los tratamientos a largo plazo más comunes para la ERC. La hemodiálisis necesita un acceso vascular, que es una conexión arteriovenosa actualmente hecha con materiales sintéticos como politetrafluoroetileno expandido (ePTFE), que tienen complicaciones importantes como hiperplasia de la íntima, infección y trombosis, debido a las propiedades químicas, biológicas y mecánicas de estos injertos en comparación con los vasos sanguíneos nativos de los pacientes. Para enfrentar las complicaciones de los injertos vasculares de ePTFE actuales, en este estudio se descelularizó la submucosa del intestino delgado (SIS por sus siglas en inglés), y se formaron tubos con este material para fabricar injertos vasculares flexibles en forma de C, de cuatro milímetros de diámetro, capaces de recibir el flujo arterial sin ninguna complicación en la arteria ni en la anastomosis venosa. Pese a que las tasas de permeabilidad y la regeneración fueron superiores en los injertos vasculares de SIS al compararlos con los de ePTFE, la trombosis se mantuvo como la principal complicación durante el proceso de maduración del injerto. Para reducir la trombogénesis, se conjugaron tres macromoléculas terminadas en carboxilo con diferentes pesos moleculares, a los grupos aminos presentes en la superficie de la SIS: fosforilcolina zwitteriónica, ácidos carboxílicos y polietilenglicol. La reducción en la deposición de plaquetas fue significativa con ácidos carboxílicos, aún mayor con macromoléculas zwitteriónicas de fosforilcolina, e incluso mejor con polietilenglicol. La PEGilación de la SIS fue fácil y económica, e incluso con un bajo índice."--Tomado del Formato de Documento de GradoDoctor en IngenieríaDoctorado174 hojasapplication/pdfengUniandesDoctorado en IngenieríaFacultad de Ingenieríainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaDesign, fabrication and evaluation of novel SIS based regenerative vascular grafts for hemodialysis accessTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesishttp://purl.org/coar/resource_type/c_db06http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TDHemodinámica - InvestigacionesFístula arteriovenosa - InvestigacionesHemodiálisis - InvestigacionesTrasplantes de órganos, tejidos, etc - InvestigacionesMedicina regenerativa - Investigaciones - Estudio de casosIngeniería de tejidos - Investigaciones - Estudio de casosIngenieríaPublicationTEXTu807590.pdf.txtu807590.pdf.txtExtracted texttext/plain309693https://repositorio.uniandes.edu.co/bitstreams/3d29510e-6ca3-45e3-a692-1121a804f59d/downloadb0aeee938d2325d421ab5d08a60f1032MD54THUMBNAILu807590.pdf.jpgu807590.pdf.jpgIM Thumbnailimage/jpeg1437https://repositorio.uniandes.edu.co/bitstreams/e9c6844d-5fe8-40fd-ba42-047dcae28672/downloadb25ea566ff387961c04cd8f398fcd6d8MD55ORIGINALu807590.pdfapplication/pdf7517268https://repositorio.uniandes.edu.co/bitstreams/3b97e59e-2908-481b-8ed0-b2e398d5e9da/download91ba9506a2f5f603b2754db1cc5d90d5MD511992/38690oai:repositorio.uniandes.edu.co:1992/386902024-08-26 15:26:35.521https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfopen.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co