Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus
Staphylococcus aureus is a Gram positive bacteria and an opportunistic pathogen responsible for a considerable amount of infections around the world, specifically it might take advantage of immunosuppressed patients in hospitals. For some years now, it has been registered that this microorganism is...
- Autores:
-
Meléndez Granados, Juan Sebastián
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2018
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/39313
- Acceso en línea:
- http://hdl.handle.net/1992/39313
- Palabra clave:
- Biofísica
Staphylococcus aureus
Estafiloxantina
Física
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.title.es_CO.fl_str_mv |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
title |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
spellingShingle |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus Biofísica Staphylococcus aureus Estafiloxantina Física |
title_short |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
title_full |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
title_fullStr |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
title_full_unstemmed |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
title_sort |
Biophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureus |
dc.creator.fl_str_mv |
Meléndez Granados, Juan Sebastián |
dc.contributor.advisor.none.fl_str_mv |
Leidy, Chad |
dc.contributor.author.none.fl_str_mv |
Meléndez Granados, Juan Sebastián |
dc.contributor.jury.none.fl_str_mv |
Forero Shelton, Antonio Manu |
dc.subject.keyword.es_CO.fl_str_mv |
Biofísica Staphylococcus aureus Estafiloxantina |
topic |
Biofísica Staphylococcus aureus Estafiloxantina Física |
dc.subject.themes.none.fl_str_mv |
Física |
description |
Staphylococcus aureus is a Gram positive bacteria and an opportunistic pathogen responsible for a considerable amount of infections around the world, specifically it might take advantage of immunosuppressed patients in hospitals. For some years now, it has been registered that this microorganism is capable of tolerating desic- cated environments like walls, furniture surfaces or even surgical instruments. For this reason, understanding the mechanisms involved in desiccation tolerance is cru- cial in strategies to fight it back. S. aureus is known for the carotenoids it syn- thetizes, specially staphyloxanthin which is the major product. These molecules have been shown to provide certain levels of protection against different kinds of stress. In this thesis, a biophysical study was carried out in order to find the role staphyloxanthin plays in desiccation tolerance. Desiccation experiments along with carotenoids concentration analysis suggests correlation between staphyloxan- thin content and the capacity of the bacteria to survive this stress. FTIR spec- troscopy measurements were performed on lipids extracted from S. aureus under different conditions to estimate their thermotropic lipid phase behavior and although it showed that lipid cooperativity may be linked to staphyloxanthin concentration, there were very few data to conclude this correlation |
publishDate |
2018 |
dc.date.issued.none.fl_str_mv |
2018 |
dc.date.accessioned.none.fl_str_mv |
2020-06-10T16:10:06Z |
dc.date.available.none.fl_str_mv |
2020-06-10T16:10:06Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/bachelorThesis |
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http://purl.org/coar/resource_type/c_7a1f |
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http://purl.org/redcol/resource_type/TP |
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http://purl.org/coar/resource_type/c_7a1f |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/39313 |
dc.identifier.pdf.none.fl_str_mv |
u821235.pdf |
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instname:Universidad de los Andes |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
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repourl:https://repositorio.uniandes.edu.co/ |
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http://hdl.handle.net/1992/39313 |
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u821235.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
dc.language.iso.es_CO.fl_str_mv |
eng |
language |
eng |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
dc.format.extent.es_CO.fl_str_mv |
44 hojas |
dc.format.mimetype.es_CO.fl_str_mv |
application/pdf |
dc.publisher.es_CO.fl_str_mv |
Universidad de los Andes |
dc.publisher.program.es_CO.fl_str_mv |
Física |
dc.publisher.faculty.es_CO.fl_str_mv |
Facultad de Ciencias |
dc.publisher.department.es_CO.fl_str_mv |
Departamento de Física |
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Universidad de los Andes |
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Repositorio Institucional Séneca |
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Repositorio Institucional Séneca |
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spelling |
Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Leidy, Chadvirtual::8464-1Meléndez Granados, Juan Sebastiánca76a271-c803-496e-ac12-7e69e03eff8b500Forero Shelton, Antonio Manu2020-06-10T16:10:06Z2020-06-10T16:10:06Z2018http://hdl.handle.net/1992/39313u821235.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Staphylococcus aureus is a Gram positive bacteria and an opportunistic pathogen responsible for a considerable amount of infections around the world, specifically it might take advantage of immunosuppressed patients in hospitals. For some years now, it has been registered that this microorganism is capable of tolerating desic- cated environments like walls, furniture surfaces or even surgical instruments. For this reason, understanding the mechanisms involved in desiccation tolerance is cru- cial in strategies to fight it back. S. aureus is known for the carotenoids it syn- thetizes, specially staphyloxanthin which is the major product. These molecules have been shown to provide certain levels of protection against different kinds of stress. In this thesis, a biophysical study was carried out in order to find the role staphyloxanthin plays in desiccation tolerance. Desiccation experiments along with carotenoids concentration analysis suggests correlation between staphyloxan- thin content and the capacity of the bacteria to survive this stress. FTIR spec- troscopy measurements were performed on lipids extracted from S. aureus under different conditions to estimate their thermotropic lipid phase behavior and although it showed that lipid cooperativity may be linked to staphyloxanthin concentration, there were very few data to conclude this correlation"Staphylococcus aureus es una bacteria Gram positiva y un patógeno oportunista responsable de una cantidad considerable de infecciones en todo el mundo, específicamente podría afectar a pacientes inmunosuprimidos en los hospitales. Desde hace algunos años, se ha registrado que este microorganismo es capaz de tolerar ambientes desecados como paredes, superficies de muebles o incluso instrumentos quirúrgicos. Por esta razón, comprender los mecanismos involucrados en la tolerancia a la desecación es crucial en las estrategias para combatirla. S. aureus es conocido por los carotenoides que sintetiza, especialmente la estafiloxantina, que es el principal producto. Estas moléculas han demostrado proporcionar ciertos niveles de protección contra diferentes tipos de estrés. En esta tesis, se realizó un estudio biofísico para determinar el papel que desempeña la estafiloxantina en la tolerancia a la desecación. Los experimentos de desecación junto con el análisis de concentración de carotenoides sugieren una correlación entre el contenido de estafiloxantina y la capacidad de las bacterias para sobrevivir a este estrés. Las mediciones de especoscopia FTIR se realizaron a partir de lípidos extraídos de S. aureus en diferentes condiciones para estimar su comportamiento de la fase lipídica termotrópica y aunque mostró que la cooperatividad de los lípidos puede estar vinculada a la concentración de estafiloxina, hubo muy pocos datos para concluir esta correlación."--Tomado del Formato de Documento de GradoFísicoPregrado44 hojasapplication/pdfengUniversidad de los AndesFísicaFacultad de CienciasDepartamento de Físicainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaBiophysical study on the role of staphyloxanthin in promoting desiccation tolerance in Staphylococcus aureusTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPBiofísicaStaphylococcus aureusEstafiloxantinaFísicaPublication29f524d9-be84-4fc7-b365-48c816849b2dvirtual::8464-129f524d9-be84-4fc7-b365-48c816849b2dvirtual::8464-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000674044virtual::8464-1THUMBNAILu821235.pdf.jpgu821235.pdf.jpgIM Thumbnailimage/jpeg7641https://repositorio.uniandes.edu.co/bitstreams/92ab7698-e8d5-462e-85c4-65ade106518b/downloada2a76f84aefd9a3bafa5c70e431cac5dMD55TEXTu821235.pdf.txtu821235.pdf.txtExtracted texttext/plain58457https://repositorio.uniandes.edu.co/bitstreams/a78cb49b-d355-4d9f-b771-5e947b69cb6c/downloade3f9be687261b5d4245cc59ffc5767faMD54ORIGINALu821235.pdfapplication/pdf14804429https://repositorio.uniandes.edu.co/bitstreams/575860f6-ebba-44b2-89b4-76172dd2496d/downloadefc441d6513220337b1faab18804eb81MD511992/39313oai:repositorio.uniandes.edu.co:1992/393132024-03-13 13:41:12.629http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |