¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT
Las lesiones de quemaduras son un problema de salud pública por lo cual su tratamiento es relevante. Una propuesta novedosa es el uso de biomateriales, tales como el ácido poli(láctico-co-glicólico) (PLGA) y la policaprolactona (PCL), capaces de promover la regeneración de tejidos. El diseño de memb...
- Autores:
-
Gómez Cárdenas, Oscar Dony
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2023
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/69050
- Acceso en línea:
- http://hdl.handle.net/1992/69050
- Palabra clave:
- Lesiones por quemaduras
Cultivo celular
Ácido poli(láctico-co-glicólico)
Policaprolactona
Células HFF-1
Células HaCaT
Microbiología
- Rights
- openAccess
- License
- Atribución-CompartirIgual 4.0 Internacional
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¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| title |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| spellingShingle |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT Lesiones por quemaduras Cultivo celular Ácido poli(láctico-co-glicólico) Policaprolactona Células HFF-1 Células HaCaT Microbiología |
| title_short |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| title_full |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| title_fullStr |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| title_full_unstemmed |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| title_sort |
¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaT |
| dc.creator.fl_str_mv |
Gómez Cárdenas, Oscar Dony |
| dc.contributor.advisor.none.fl_str_mv |
Baldrich Mora, Laura Mercedes Groot De Restrepo, Helena |
| dc.contributor.author.none.fl_str_mv |
Gómez Cárdenas, Oscar Dony |
| dc.contributor.researchgroup.es_CO.fl_str_mv |
Laboratorio de Genética Humana |
| dc.subject.keyword.none.fl_str_mv |
Lesiones por quemaduras Cultivo celular Ácido poli(láctico-co-glicólico) Policaprolactona Células HFF-1 Células HaCaT |
| topic |
Lesiones por quemaduras Cultivo celular Ácido poli(láctico-co-glicólico) Policaprolactona Células HFF-1 Células HaCaT Microbiología |
| dc.subject.themes.es_CO.fl_str_mv |
Microbiología |
| description |
Las lesiones de quemaduras son un problema de salud pública por lo cual su tratamiento es relevante. Una propuesta novedosa es el uso de biomateriales, tales como el ácido poli(láctico-co-glicólico) (PLGA) y la policaprolactona (PCL), capaces de promover la regeneración de tejidos. El diseño de membranas con estos biomateriales debería ser capaz de proporcionar un soporte sólido sobre el cual crezcan adheridas las células esenciales en la regeneración de la piel, especialmente los fibroblastos y los queratinocitos. Por lo tanto, se evaluó el efecto de membranas de PLGA y PLGA + PCL como soportes que potencien el crecimiento de células HFF-1 (fibroblastos) y HaCaT (queratinocitos). De esta manera, se realizó una primera aproximación en aras de establecer una potencial aplicación para mejorar la calidad de vida en pacientes con lesiones por quemaduras. |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-02T14:10:24Z |
| dc.date.available.none.fl_str_mv |
2023-08-02T14:10:24Z |
| dc.date.issued.none.fl_str_mv |
2023-06-06 |
| dc.type.es_CO.fl_str_mv |
Trabajo de grado - Pregrado |
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info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/acceptedVersion |
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Burns. https://doi.org/10.1016/j.burns.2023.02.007 Zhong, J., Wang, H., Yang, K., Wang, H., Duan, C., Ni, N., An, L., Luo, Y., Zhao, P., Gou, Y., Sheng, S., Shi, D., Chen, C., Wagstaff, W., Hendren-Santiago, B., Haydon, R. C., Luu, H. H., Reid, R. R., Ho, S. H., ... Fan, J. (2022). Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering. Bioactive Materials, 9, 523-540. https://doi.org/10.1016/j.bioactmat.2021.07.022 Zorina, A., Zorin, V., Isaev, A., Kudlay, D., Vasileva, M., & Kopnin, P. (2023). Dermal Fibroblasts as the Main Target for Skin Anti-Age Correction Using a Combination of Regenerative Medicine Methods. En Current Issues in Molecular Biology (Vol. 45, Número 5, pp. 3829-3847). https://doi.org/10.3390/cimb45050247 |
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Atribución-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Baldrich Mora, Laura Mercedes28851a50-3193-456a-9efa-e2bc010a2fce600Groot De Restrepo, Helenavirtual::4050-1Gómez Cárdenas, Oscar Dony8de8d0c1-97b6-4b3b-83af-f447ac09eef1600Laboratorio de Genética Humana2023-08-02T14:10:24Z2023-08-02T14:10:24Z2023-06-06http://hdl.handle.net/1992/69050instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Las lesiones de quemaduras son un problema de salud pública por lo cual su tratamiento es relevante. Una propuesta novedosa es el uso de biomateriales, tales como el ácido poli(láctico-co-glicólico) (PLGA) y la policaprolactona (PCL), capaces de promover la regeneración de tejidos. El diseño de membranas con estos biomateriales debería ser capaz de proporcionar un soporte sólido sobre el cual crezcan adheridas las células esenciales en la regeneración de la piel, especialmente los fibroblastos y los queratinocitos. Por lo tanto, se evaluó el efecto de membranas de PLGA y PLGA + PCL como soportes que potencien el crecimiento de células HFF-1 (fibroblastos) y HaCaT (queratinocitos). De esta manera, se realizó una primera aproximación en aras de establecer una potencial aplicación para mejorar la calidad de vida en pacientes con lesiones por quemaduras.MicrobiólogoPregrado24 páginasapplication/pdfspaUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias Biológicas¿Uso de membranas con ácido poli(láctico-co-glicólico) y con policaprolactona para el tratamiento de lesiones por quemaduras? Primera aproximación evaluando el crecimiento de células HFF-1 y HaCaTTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPLesiones por quemadurasCultivo celularÁcido poli(láctico-co-glicólico)PolicaprolactonaCélulas HFF-1Células HaCaTMicrobiologíaAnimal Cell Culture Guide | ATCC. (s/f). https://www.atcc.org/resources/culture-guides/animal-cell-culture-guideBabilotte, J., Martin, B., Guduric, V., Bareille, R., Agniel, R., Roques, S., Héroguez, V., Dussauze, M., Gaudon, M., Le Nihouannen, D., & Catros, S. (2021). Development and characterization of a PLGA-HA composite material to fabricate 3D-printed scaffolds for bone tissue engineering. Materials Science and Engineering: C, 118, 111334. https://doi.org/10.1016/j.msec.2020.111334Baust, J. M., Buehring, G. C., Campbell, L., Elmore, E., Harbell, J. W., Nims, R. W., Price, P., Reid, Y. A., & Simione, F. (2017). Best practices in cell culture: an overview. In Vitro Cellular & Developmental Biology - Animal, 53(8), 669-672. https://doi.org/10.1007/s11626-017-0177-7Bédard, P., Gauvin, S., Ferland, K., Caneparo, C., Pellerin, È., Chabaud, S., & Bolduc, S. (2020). Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing. Bioengineering (Basel, Switzerland), 7(3). https://doi.org/10.3390/bioengineering7030115Cai, S., Wu, C., Yang, W., Liang, W., Yu, H., & Liu, L. (2020). Recent advance in surface modification for regulating cell adhesion and behaviors. 9(1), 971-989. https://doi.org/10.1515/ntrev-2020-0076Chen, G., Sato, T., Ohgushi, H., Ushida, T., Tateishi, T., & Tanaka, J. (2005). Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh. Biomaterials, 26(15), 2559-2566. https://doi.org/10.1016/j.biomaterials.2004.07.034Chen, H., Yin, B., Hu, B., Zhang, B., Liu, J., Jing, Y., Fan, Z., Tian, Y., Wei, X., & Zhang, W. (2021). Acellular fish skin enhances wound healing by promoting angiogenesis and collagen deposition. Biomedical Materials, 16(4), 45011. https://doi.org/10.1088/1748-605X/abef7aChoi, W. S., Kim, J. H., Ahn, C. B., Lee, J. H., Kim, Y. J., Son, K. H., & Lee, J. W. (2021). Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing. Polymers, 13(16). https://doi.org/10.3390/polym13162584Cialdai, F., Risaliti, C., & Monici, M. (2022). Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Frontiers in Bioengineering and Biotechnology, 10, 958381. https://doi.org/10.3389/fbioe.2022.958381Compton, T. (1993). An immortalized human fibroblast cell line is permissive for human cytomegalovirus infection. Journal of Virology, 67(6), 3644-3648. https://doi.org/10.1128/JVI.67.6.3644-3648.1993Costa-Almeida, R., Gomez-Lazaro, M., Ramalho, C., Granja, P. L., Soares, R., & Guerreiro, S. G. (2015). 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Anais Brasileiros de Dermatologia, 97(5), 575-582. https://doi.org/10.1016/j.abd.2021.09.012Gentile, P., Chiono, V., Carmagnola, I., & Hatton, P. V. (2014). An overview of poly(lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering. International Journal of Molecular Sciences, 15(3), 3640-3659. https://doi.org/10.3390/ijms15033640Gharibshahian, M., Salehi, M., Beheshtizadeh, N., Kamalabadi-Farahani, M., Atashi, A., Nourbakhsh, M.-S., & Alizadeh, M. (2023). Recent advances on 3D-printed PCL-based composite scaffolds for bone tissue engineering . En Frontiers in Bioengineering and Biotechnology (Vol. 11). https://www.frontiersin.org/articles/10.3389/fbioe.2023.1168504González-González, A. M., Cruz, R., Rosales-Ibáñez, R., Hernández-Sánchez, F., Carrillo-Escalante, H. J., Rodríguez-Martínez, J. J., Velasquillo, C., Talamás-Lara, D., & Ludert, J. E. (2023). In Vitro and In Vivo Evaluation of a Polycaprolactone (PCL)/Polylactic-Co-Glycolic Acid (PLGA) (80:20) Scaffold for Improved Treatment of Chondral (Cartilage) Injuries. En Polymers (Vol. 15, Número 10). https://doi.org/10.3390/polym15102324Greenhalgh, D. G. (2019). Management of Burns. New England Journal of Medicine, 380(24), 2349-2359. https://doi.org/10.1056/NEJMra1807442Hama, R., Reinhardt, J. W., Ulziibayar, A., Watanabe, T., Kelly, J., & Shinoka, T. (2023). Recent Tissue Engineering Approaches to Mimicking the Extracellular Matrix Structure for Skin Regeneration. En Biomimetics (Vol. 8, Número 1). https://doi.org/10.3390/biomimetics8010130Han, Y., Lian, M., Wu, Q., Qiao, Z., Sun, B., & Dai, K. (2021). Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds. En Frontiers in Bioengineering and Biotechnology (Vol. 9). https://www.frontiersin.org/articles/10.3389/fbioe.2021.629270Hosseini Mansoub, N. (2021). 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Re-epithelialization of adult skin wounds: Cellular mechanisms and therapeutic strategies. Advanced Drug Delivery Reviews, 146, 344-365. https://doi.org/0.1016/j.addr.2018.06.019Russo, B., Brembilla, N. C., & Chizzolini, C. (2020). Interplay Between Keratinocytes and Fibroblasts: A Systematic Review Providing a New Angle for Understanding Skin Fibrotic Disorders. En Frontiers in immunology (Vol. 11, p. 648). https://doi.org/10.3389/fimmu.2020.00648Sadeghi-Avalshahr, A., Nokhasteh, S., Molavi, A. M., Khorsand-Ghayeni, M., & Mahdavi-Shahri, M. (2017). Synthesis and characterization of collagen/PLGA biodegradable skin scaffold fibers. Regenerative Biomaterials, 4(5), 309-314. https://doi.org/10.1093/rb/rbx026Salerno, S., Messina, A., Giordano, F., Bader, A., Drioli, E., & De Bartolo, L. (2017). Dermal-epidermal membrane systems by using human keratinocytes and mesenchymal stem cells isolated from dermis. 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