Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur

Malassezia genus is composed by 17 different yeast species. It is a common yeast in the skin microbiota and it is often a commensal fungi incapable to cause damage in the host. However, some species are related with cutaneous and systemic diseases. Previous studies reported some target enzymes to in...

Full description

Autores:
Pérez Franco, Diana Carolina
Triana Sierra, Sergio Helí
Celis Ramírez, Adriana Marcela
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2018
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/60876
Acceso en línea:
http://hdl.handle.net/1992/60876
Palabra clave:
Enzimas
Malassezia furfur
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/4.0/
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2González Barrios, Andrés Fernandocc132031-adde-4342-ba8c-b406b8175e2b400Pérez Franco, Diana Carolinac535ff5c-4959-4e8a-9524-18223fb761d7500Triana Sierra, Sergio Helía53768f6-2e3c-4797-9dfa-6797e96a66e4500Celis Ramírez, Adriana Marcela7806500Gómez Ramírez, Jorge Mario2022-09-26T22:03:15Z2022-09-26T22:03:15Z2018http://hdl.handle.net/1992/60876instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/795786-1001Malassezia genus is composed by 17 different yeast species. It is a common yeast in the skin microbiota and it is often a commensal fungi incapable to cause damage in the host. However, some species are related with cutaneous and systemic diseases. Previous studies reported some target enzymes to inhibit the growth of Malassezia spp., one of those is homoserine dehydrogenase. For that reason, the present research sought to purify and quantify the enzymatic activity of homoserine dehydrogenase with the future aim to prove the inhibition of Malassezia furfur growth by the inhibition of the enzyme. To carry out the purification and measurement of enzymatic activity, E. coli with a specific his-tagged clone of homoserine dehydrogenase was sow in LB agar. The clone expression was induced by IPTG 2mM in the third hour of incubation and then the culture was purified using 2 different techniques: affinity chromatography and dynabeads...El género Malassezia está compuesto por 17 diferentes especies de levaduras. Este organismo es común en la microbiota de la piel y a menudo es un hongo comensal incapaz de causar daño en el hospedero. Sin embargo, algunas especies de Malassezia están relacionadas con enfermedades cutáneas y sistémicas. Estudios previos han reportado posibles enzimas blanco para inhibir el crecimiento de Malassezia spp., una de ellas es la enzima homoserina deshidrogenasa. Por tal razón, el presente trabajo busca purificar y cuantificar la actividad enzimática de homoserina deshidrogenasa con el objetivo futuro de probar la inhibición del crecimiento de Malassezia furfur mediante la inhibición de dicha enzima. Para llevar a cabo la purificación y medición de actividad enzimática se sembró E. coli en agar LB cuyo genoma contenía un clon codificante para homoserina deshidrogenasa y colas de histidina. La expresión del gen fue inducida con IPTG 2mM en la tercera hora de incubación...Ingeniero QuímicoMicrobiólogoPregrado8 hojasapplication/pdfengUniversidad de los AndesIngeniería QuímicaMicrobiologíaFacultad de IngenieríaFacultad de CienciasDepartamento de Ingeniería Química y de AlimentosDepartamento de Ciencias BiológicasPurification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfurTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPEnzimasMalassezia furfur201315055PublicationTHUMBNAIL13169.pdf.jpg13169.pdf.jpgIM Thumbnailimage/jpeg25893https://repositorio.uniandes.edu.co/bitstreams/f228b4bc-2afc-4b4c-ad88-5ec3e580b7eb/downloada57f2f711257b1b2f330842bfecd0c36MD53TEXT13169.pdf.txt13169.pdf.txtExtracted texttext/plain29221https://repositorio.uniandes.edu.co/bitstreams/17930c44-883f-4ddc-8270-70a5a09b6dea/download9d10cb84a30c3bd5d4f8ae768bb1cdf2MD52ORIGINAL13169.pdfapplication/pdf779303https://repositorio.uniandes.edu.co/bitstreams/73e329de-e561-442e-aa64-b870b66c2a23/downloadee29b0ad25661452cde829bcd264c413MD511992/60876oai:repositorio.uniandes.edu.co:1992/608762023-10-10 19:15:20.768http://creativecommons.org/licenses/by-nc-nd/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co
dc.title.spa.fl_str_mv Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
title Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
spellingShingle Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
Enzimas
Malassezia furfur
title_short Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
title_full Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
title_fullStr Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
title_full_unstemmed Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
title_sort Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
dc.creator.fl_str_mv Pérez Franco, Diana Carolina
Triana Sierra, Sergio Helí
Celis Ramírez, Adriana Marcela
dc.contributor.advisor.none.fl_str_mv González Barrios, Andrés Fernando
dc.contributor.author.none.fl_str_mv Pérez Franco, Diana Carolina
Triana Sierra, Sergio Helí
Celis Ramírez, Adriana Marcela
dc.contributor.jury.none.fl_str_mv Gómez Ramírez, Jorge Mario
dc.subject.keyword.spa.fl_str_mv Enzimas
Malassezia furfur
topic Enzimas
Malassezia furfur
description Malassezia genus is composed by 17 different yeast species. It is a common yeast in the skin microbiota and it is often a commensal fungi incapable to cause damage in the host. However, some species are related with cutaneous and systemic diseases. Previous studies reported some target enzymes to inhibit the growth of Malassezia spp., one of those is homoserine dehydrogenase. For that reason, the present research sought to purify and quantify the enzymatic activity of homoserine dehydrogenase with the future aim to prove the inhibition of Malassezia furfur growth by the inhibition of the enzyme. To carry out the purification and measurement of enzymatic activity, E. coli with a specific his-tagged clone of homoserine dehydrogenase was sow in LB agar. The clone expression was induced by IPTG 2mM in the third hour of incubation and then the culture was purified using 2 different techniques: affinity chromatography and dynabeads...
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2022-09-26T22:03:15Z
dc.date.available.none.fl_str_mv 2022-09-26T22:03:15Z
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dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
dc.identifier.local.spa.fl_str_mv 795786-1001
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dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
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eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 8 hojas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Ingeniería Química
Microbiología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ingeniería
Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Ingeniería Química y de Alimentos
Departamento de Ciencias Biológicas
institution Universidad de los Andes
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