Purification and measurement of enzymatic activity and kinetic parameters for homoserine dehydrogenase as therapeutic target in Malassezia furfur
Malassezia genus is composed by 17 different yeast species. It is a common yeast in the skin microbiota and it is often a commensal fungi incapable to cause damage in the host. However, some species are related with cutaneous and systemic diseases. Previous studies reported some target enzymes to in...
- Autores:
-
Pérez Franco, Diana Carolina
Triana Sierra, Sergio Helí
Celis Ramírez, Adriana Marcela
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2018
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/60876
- Acceso en línea:
- http://hdl.handle.net/1992/60876
- Palabra clave:
- Enzimas
Malassezia furfur
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
| Summary: | Malassezia genus is composed by 17 different yeast species. It is a common yeast in the skin microbiota and it is often a commensal fungi incapable to cause damage in the host. However, some species are related with cutaneous and systemic diseases. Previous studies reported some target enzymes to inhibit the growth of Malassezia spp., one of those is homoserine dehydrogenase. For that reason, the present research sought to purify and quantify the enzymatic activity of homoserine dehydrogenase with the future aim to prove the inhibition of Malassezia furfur growth by the inhibition of the enzyme. To carry out the purification and measurement of enzymatic activity, E. coli with a specific his-tagged clone of homoserine dehydrogenase was sow in LB agar. The clone expression was induced by IPTG 2mM in the third hour of incubation and then the culture was purified using 2 different techniques: affinity chromatography and dynabeads... |
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