Assessment of the synergic effect between Lysinibacillus sphaericus S-Layer protein and Glyphosate in the lethality of the invasive arboviral vector Aedes albopictus

Glyphosate and glyphosate-based herbicides are among the most used chemicals in plant pest control. Both glyphosate and its main by-product, Aminomethylphosphonic Acid (AMPA) are highly environmentally persistent, and through several processes (including surface runoff and bioaccumulation) affect non-target species beyond the Plantae kingdom, especially in aquatic ecosystems. Aedes albopictus is a novel invasive arboviral vector in Colombia and has spread to much of the national territory in recent years. Strains of the bacterium Lysinibacillus sphaericus have shown the ability to degrade glyphosate into environmentally inert compounds, in addition to having great larvicidal efficiency in different mosquito species through the production of several proteins, including the Surface layer (S-layer) protein. The S-layer is a bacterial structure conformed by glycoprotein monomers, and its functions are thought to include bacterial interactions, protection from the outside medium and biological control. The study assessed the entomopathogenic activity of L. sphaericus S-layer protein on Ae. albopictus larvae, and the effects that glyphosate and its by-products have in this process. To that end, bioassays were performed to compare the larval mortality between different treatments with and without S-layer, glyphosate, and glyphosate-derivates. Comparisons were made through ANOVA analysis

• Autores: Dániel Gómez, Mario Felipe
• Tipo de recurso: Trabajo de grado de pregrado
• Fecha de publicación: 2020
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: eng
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/48888
• Acceso en línea: http://hdl.handle.net/1992/48888
• Palabra clave: Lysinibacillus sphaericus; Bacterias grampositivas; Glifosato; Aedes albopictus; Control biológico de plagas; Microbiología
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/4.0/