Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles
Neocosmospora and Fusarium are the etiological agents of human fusariosis such as onychomycosis, fungal keratitis and disseminated infections, and the most widely reported species are Neocosmospora keratoplastica, Neocosmospora falciformis and Fusarium oxysporum. In addition, they are resistance to...
- Autores:
-
Lizcano Salas, Andrés Felipe
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2021
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/51317
- Acceso en línea:
- http://hdl.handle.net/1992/51317
- Palabra clave:
- Agentes antifungosos
Fusarium solani
Resistencia a drogas en microorganismos
Fusarium Oxysporum
Micología médica
Microbiología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| title |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| spellingShingle |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles Agentes antifungosos Fusarium solani Resistencia a drogas en microorganismos Fusarium Oxysporum Micología médica Microbiología |
| title_short |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| title_full |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| title_fullStr |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| title_full_unstemmed |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| title_sort |
Mutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azoles |
| dc.creator.fl_str_mv |
Lizcano Salas, Andrés Felipe |
| dc.contributor.advisor.none.fl_str_mv |
Celis Ramírez, Adriana Marcela |
| dc.contributor.author.none.fl_str_mv |
Lizcano Salas, Andrés Felipe |
| dc.subject.armarc.none.fl_str_mv |
Agentes antifungosos Fusarium solani Resistencia a drogas en microorganismos Fusarium Oxysporum Micología médica |
| topic |
Agentes antifungosos Fusarium solani Resistencia a drogas en microorganismos Fusarium Oxysporum Micología médica Microbiología |
| dc.subject.themes.none.fl_str_mv |
Microbiología |
| description |
Neocosmospora and Fusarium are the etiological agents of human fusariosis such as onychomycosis, fungal keratitis and disseminated infections, and the most widely reported species are Neocosmospora keratoplastica, Neocosmospora falciformis and Fusarium oxysporum. In addition, they are resistance to azole compounds. Resistance to azoles has been mainly associated with mutations of the CYP51A gene. Additionally, other mechanisms have been determined such as mutations in the erg5 and erg3 genes in Candida or mutations in hmg1 and hapE in Aspergillus fumigatus. In Neocosmospora and F. oxysporum, the expression of different genes in response to azoles has been mainly evaluated; and CYP51A mutations in Neocosmospora keratoplastica. However, it is unknown whether resistance is associated with other mechanisms in Neocosmospora solani and F. oxysporum. Therefore, the objective of this study is to understand the mechanisms of resistance to azoles, by analyzing the genes CYP51A, erg3, erg5, hmg1 and hap5 in N. solani FMR 4391 and F. oxysporum FMR 9788. By means of the protocol of Calling variants gatk for RNA-seq and the libraries obtained by Castillo et al. (2020), variant verification was performed. It was found that there were no variants related to azole resistance in erg3, erg5, hmg1 and hap5. In addition, the R30Q mutation in N. solani CYP51A is reported and proposed as a mechanism of resistance to azoles, and the amino acid L218, which has been previously associated with resistance to posaconazole and itraconazole, is reported. In conclusion, the R30Q mutation is proposed as a mechanism of resistance to azoles in N. solani, however more studies are needed to confirm its relevance in resistance. |
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2021 |
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2021-08-10T18:20:01Z |
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2021-08-10T18:20:01Z |
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2021 |
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Trabajo de grado - Pregrado |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/bachelorThesis |
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http://hdl.handle.net/1992/51317 |
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23075.pdf |
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repourl:https://repositorio.uniandes.edu.co/ |
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http://hdl.handle.net/1992/51317 |
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16 hojas |
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Universidad de los Andes |
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Microbiología |
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Facultad de Ciencias |
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Departamento de Ciencias Biológicas |
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Universidad de los Andes |
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Universidad de los Andes |
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Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Celis Ramírez, Adriana Marcelavirtual::8766-1Lizcano Salas, Andrés Felipe91f1c5fc-5347-4f80-9a88-3ba19efc35b75002021-08-10T18:20:01Z2021-08-10T18:20:01Z2021http://hdl.handle.net/1992/5131723075.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Neocosmospora and Fusarium are the etiological agents of human fusariosis such as onychomycosis, fungal keratitis and disseminated infections, and the most widely reported species are Neocosmospora keratoplastica, Neocosmospora falciformis and Fusarium oxysporum. In addition, they are resistance to azole compounds. Resistance to azoles has been mainly associated with mutations of the CYP51A gene. Additionally, other mechanisms have been determined such as mutations in the erg5 and erg3 genes in Candida or mutations in hmg1 and hapE in Aspergillus fumigatus. In Neocosmospora and F. oxysporum, the expression of different genes in response to azoles has been mainly evaluated; and CYP51A mutations in Neocosmospora keratoplastica. However, it is unknown whether resistance is associated with other mechanisms in Neocosmospora solani and F. oxysporum. Therefore, the objective of this study is to understand the mechanisms of resistance to azoles, by analyzing the genes CYP51A, erg3, erg5, hmg1 and hap5 in N. solani FMR 4391 and F. oxysporum FMR 9788. By means of the protocol of Calling variants gatk for RNA-seq and the libraries obtained by Castillo et al. (2020), variant verification was performed. It was found that there were no variants related to azole resistance in erg3, erg5, hmg1 and hap5. In addition, the R30Q mutation in N. solani CYP51A is reported and proposed as a mechanism of resistance to azoles, and the amino acid L218, which has been previously associated with resistance to posaconazole and itraconazole, is reported. In conclusion, the R30Q mutation is proposed as a mechanism of resistance to azoles in N. solani, however more studies are needed to confirm its relevance in resistance.Neocosmospora y Fusarium son los agentes etiológicos de las fusariosis humanas como onicomicosis, queratitis fúngicas e infecciones diseminadas, y las especies de mayor reporte son Neocosmospora keratoplastica, Neocosmospora falciformis y Fusarium oxysporum. Además, presentan resistencia a compuestos azoles. La resistencia a azoles se ha asociado principalmente a mutaciones del gen CYP51A. Adicionalmente, se han determinado otros mecanismos como mutaciones en los genes erg5 y erg3 en Candida o mutaciones en hmg1 y hapE en Aspergillus fumigatus. En Neocosmospora y F. oxysporum se han evaluado principalmente la expresión de distintos genes en respuesta a azoles; y mutaciones en CYP51A en Neocosmospora keratoplastica. Sin embargo, se desconoce si la resistencia está asociada con otros mecanismos en Neocosmospora solani y F. oxysporum. Por lo tanto, el objetivo de este estudio es comprender los mecanismos de resistencia a azoles, mediante el análisis de los genes CYP51A, erg3, erg5, hmg1 y hap5 en N. solani FMR 4391 y F. oxysporum FMR 9788. Por medio del protocolo de llamado de variantes de gatk para RNA-seq y las librerías obtenidas por Castillo y colaboradores (2020), se realizó la verificación de variantes. Se obtuvo que no hubo variantes relacionadas con resistencia a azoles en erg3, erg5, hmg1 y hap5. Además, se reporta y propone la mutación R30Q en CYP51A de N. solani como mecanismo de resistencia a azoles y se reporta el aminoácido L218 que se ha asociado previamente con resistencia a posaconazol e itraconazol. En conclusión, se propone la mutación R30Q como mecanismo de resistencia a azoles en N. solani, sin embargo, se necesitan más estudios para confirmar su relevancia en la resistencia.MicrobiólogoPregrado16 hojasapplication/pdfspaUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasMutación r30q en cyp51a de neocosmospora solani asociada a resistencia a azolesTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPAgentes antifungososFusarium solaniResistencia a drogas en microorganismosFusarium OxysporumMicología médicaMicrobiología201718005Publication0000-0003-3057-1966virtual::8766-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000178225virtual::8766-17ec7a7b4-b510-4816-b236-023aaa0754f0virtual::8766-17ec7a7b4-b510-4816-b236-023aaa0754f0virtual::8766-1TEXT23075.pdf.txt23075.pdf.txtExtracted texttext/plain41340https://repositorio.uniandes.edu.co/bitstreams/9272d5a6-7a28-4926-820a-84a6ca4241d9/download3277ae3c020f6d9fdf9ba286100e8dbaMD54ORIGINAL23075.pdfapplication/pdf437839https://repositorio.uniandes.edu.co/bitstreams/209d667e-a454-4a6d-8fb9-4ae7bb4e34e8/downloada4d674419be01922478252113196b92bMD51THUMBNAIL23075.pdf.jpg23075.pdf.jpgIM Thumbnailimage/jpeg24204https://repositorio.uniandes.edu.co/bitstreams/40e0e48b-713c-44ff-b3e1-7f4dc8d41474/downloade3b92e474a7764472d628750eb48af4cMD551992/51317oai:repositorio.uniandes.edu.co:1992/513172024-11-14 14:50:03.407http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |