Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemi...
- Autores:
-
Lara Gutiérrez, Juanita María
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2014
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/62103
- Acceso en línea:
- http://hdl.handle.net/1992/62103
- Palabra clave:
- Bacillus subtilis
Lípidos
Biología
- Rights
- openAccess
- License
- Atribución-CompartirIgual 4.0 Internacional
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Atribución-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Leidy, Chad2b60d701-94f4-4ef0-8e4c-e38fc520e479500Bernal Giraldo, Adriana Jimenavirtual::17646-1Lara Gutiérrez, Juanita María6a364fcf-9548-4b24-a3ce-3a57745f91d25002022-09-30T14:43:36Z2022-09-30T14:43:36Z2014http://hdl.handle.net/1992/62103instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/672014-1001Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemical membrane processes. For this reason, the appropriate physical conditions are required in order to maintain the ideal physiological state of the cells. In this study, the changes in lipid chain order during cold shock in the bacterium Bacillus subtilis were studied by means of the quantification of Generalized Polarization (GP) using the fluorescent probe di-4-ANEPPDHQ in vivo and the expression levels of the desaturase [delta]-5 des (as GFP fluorescence) were measured to determine its role as the primary response mechanism to restore membrane fluidity when temperaure is lowered. The data obtained from the GP quantification presented high levels of noise, but a recovery in membrane fluidity was discernible. This result appeared approximately at the time when the GFP levels peaked, showing that the lipid chain modification might be a consequence of [delta]-5 des activity. Additionally, the fusion of the [delta]-5 des promoter with the Enhanced Cyan Fluorescent Protein (ECFP) coding sequence modified with an LVA tail for rapid degradation of the protein was cloned in a vector that enables ectopic integration of the sequence in the chromosome...BiólogoPregrado11 páginasapplication/pdfengUniversidad de los AndesBiologíaFacultad de CienciasDepartamento de Ciencias BiológicasCharacterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 desTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPBacillus subtilisLípidosBiologíaPublicationhttps://scholar.google.es/citations?user=vQ9yFZoAAAAJvirtual::17646-10000-0002-3557-697Xvirtual::17646-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000622354virtual::17646-14e93f81c-d517-4226-80b7-2c5d693a24f4virtual::17646-14e93f81c-d517-4226-80b7-2c5d693a24f4virtual::17646-1THUMBNAIL200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.jpg200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.jpgIM Thumbnailimage/jpeg8659https://repositorio.uniandes.edu.co/bitstreams/3779550a-b8f5-4f33-8d91-cd4e26d2d090/download08f810edca6d9982a5936ff978e08227MD53TEXT200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.txt200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.txtExtracted texttext/plain27315https://repositorio.uniandes.edu.co/bitstreams/b29de729-7191-4faa-87e1-ff96070082d5/download9f64b49156e8e7f59bfd88b28588c6a8MD52ORIGINAL200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdfPDFapplication/pdf1577475https://repositorio.uniandes.edu.co/bitstreams/2e619e29-1337-415d-ad65-72074eef9cda/download40dbfb9db96b9619c97b3062b915f8cfMD511992/62103oai:repositorio.uniandes.edu.co:1992/621032024-03-13 16:04:41.626http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |
dc.title.spa.fl_str_mv |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
title |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
spellingShingle |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des Bacillus subtilis Lípidos Biología |
title_short |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
title_full |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
title_fullStr |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
title_full_unstemmed |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
title_sort |
Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des |
dc.creator.fl_str_mv |
Lara Gutiérrez, Juanita María |
dc.contributor.advisor.none.fl_str_mv |
Leidy, Chad Bernal Giraldo, Adriana Jimena |
dc.contributor.author.none.fl_str_mv |
Lara Gutiérrez, Juanita María |
dc.subject.keyword.none.fl_str_mv |
Bacillus subtilis Lípidos |
topic |
Bacillus subtilis Lípidos Biología |
dc.subject.themes.spa.fl_str_mv |
Biología |
description |
Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemical membrane processes. For this reason, the appropriate physical conditions are required in order to maintain the ideal physiological state of the cells. In this study, the changes in lipid chain order during cold shock in the bacterium Bacillus subtilis were studied by means of the quantification of Generalized Polarization (GP) using the fluorescent probe di-4-ANEPPDHQ in vivo and the expression levels of the desaturase [delta]-5 des (as GFP fluorescence) were measured to determine its role as the primary response mechanism to restore membrane fluidity when temperaure is lowered. The data obtained from the GP quantification presented high levels of noise, but a recovery in membrane fluidity was discernible. This result appeared approximately at the time when the GFP levels peaked, showing that the lipid chain modification might be a consequence of [delta]-5 des activity. Additionally, the fusion of the [delta]-5 des promoter with the Enhanced Cyan Fluorescent Protein (ECFP) coding sequence modified with an LVA tail for rapid degradation of the protein was cloned in a vector that enables ectopic integration of the sequence in the chromosome... |
publishDate |
2014 |
dc.date.issued.spa.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2022-09-30T14:43:36Z |
dc.date.available.none.fl_str_mv |
2022-09-30T14:43:36Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TP |
format |
http://purl.org/coar/resource_type/c_7a1f |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/62103 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad de los Andes |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repositorio.uniandes.edu.co/ |
dc.identifier.local.spa.fl_str_mv |
672014-1001 |
url |
http://hdl.handle.net/1992/62103 |
identifier_str_mv |
instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ 672014-1001 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.rights.license.spa.fl_str_mv |
Atribución-CompartirIgual 4.0 Internacional |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.extent.spa.fl_str_mv |
11 páginas |
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application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad de los Andes |
dc.publisher.program.spa.fl_str_mv |
Biología |
dc.publisher.faculty.spa.fl_str_mv |
Facultad de Ciencias |
dc.publisher.department.spa.fl_str_mv |
Departamento de Ciencias Biológicas |
institution |
Universidad de los Andes |
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