Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des

Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemi...

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Autores:
Lara Gutiérrez, Juanita María
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2014
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/62103
Acceso en línea:
http://hdl.handle.net/1992/62103
Palabra clave:
Bacillus subtilis
Lípidos
Biología
Rights
openAccess
License
Atribución-CompartirIgual 4.0 Internacional
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spelling Atribución-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Leidy, Chad2b60d701-94f4-4ef0-8e4c-e38fc520e479500Bernal Giraldo, Adriana Jimenavirtual::17646-1Lara Gutiérrez, Juanita María6a364fcf-9548-4b24-a3ce-3a57745f91d25002022-09-30T14:43:36Z2022-09-30T14:43:36Z2014http://hdl.handle.net/1992/62103instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/672014-1001Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemical membrane processes. For this reason, the appropriate physical conditions are required in order to maintain the ideal physiological state of the cells. In this study, the changes in lipid chain order during cold shock in the bacterium Bacillus subtilis were studied by means of the quantification of Generalized Polarization (GP) using the fluorescent probe di-4-ANEPPDHQ in vivo and the expression levels of the desaturase [delta]-5 des (as GFP fluorescence) were measured to determine its role as the primary response mechanism to restore membrane fluidity when temperaure is lowered. The data obtained from the GP quantification presented high levels of noise, but a recovery in membrane fluidity was discernible. This result appeared approximately at the time when the GFP levels peaked, showing that the lipid chain modification might be a consequence of [delta]-5 des activity. Additionally, the fusion of the [delta]-5 des promoter with the Enhanced Cyan Fluorescent Protein (ECFP) coding sequence modified with an LVA tail for rapid degradation of the protein was cloned in a vector that enables ectopic integration of the sequence in the chromosome...BiólogoPregrado11 páginasapplication/pdfengUniversidad de los AndesBiologíaFacultad de CienciasDepartamento de Ciencias BiológicasCharacterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 desTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPBacillus subtilisLípidosBiologíaPublicationhttps://scholar.google.es/citations?user=vQ9yFZoAAAAJvirtual::17646-10000-0002-3557-697Xvirtual::17646-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000622354virtual::17646-14e93f81c-d517-4226-80b7-2c5d693a24f4virtual::17646-14e93f81c-d517-4226-80b7-2c5d693a24f4virtual::17646-1THUMBNAIL200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.jpg200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.jpgIM Thumbnailimage/jpeg8659https://repositorio.uniandes.edu.co/bitstreams/3779550a-b8f5-4f33-8d91-cd4e26d2d090/download08f810edca6d9982a5936ff978e08227MD53TEXT200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.txt200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf.txtExtracted texttext/plain27315https://repositorio.uniandes.edu.co/bitstreams/b29de729-7191-4faa-87e1-ff96070082d5/download9f64b49156e8e7f59bfd88b28588c6a8MD52ORIGINAL200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdf200723803_fecha_2014_01_24_hora_17_58_34_parte_1.pdfPDFapplication/pdf1577475https://repositorio.uniandes.edu.co/bitstreams/2e619e29-1337-415d-ad65-72074eef9cda/download40dbfb9db96b9619c97b3062b915f8cfMD511992/62103oai:repositorio.uniandes.edu.co:1992/621032024-03-13 16:04:41.626http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co
dc.title.spa.fl_str_mv Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
title Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
spellingShingle Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
Bacillus subtilis
Lípidos
Biología
title_short Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
title_full Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
title_fullStr Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
title_full_unstemmed Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
title_sort Characterization of the bacillus subtilis response to changes in temperature by the activity of the enzyme desaturase [delta]-5 des
dc.creator.fl_str_mv Lara Gutiérrez, Juanita María
dc.contributor.advisor.none.fl_str_mv Leidy, Chad
Bernal Giraldo, Adriana Jimena
dc.contributor.author.none.fl_str_mv Lara Gutiérrez, Juanita María
dc.subject.keyword.none.fl_str_mv Bacillus subtilis
Lípidos
topic Bacillus subtilis
Lípidos
Biología
dc.subject.themes.spa.fl_str_mv Biología
description Lipids provide the ideal matrix and the right physical conditions for many proteins that are in charge of many cellualr functions. However, lipid membranes not only represent the scenario on which cellular functions are carried out, it has also been argued that lipids play an active role in biochemical membrane processes. For this reason, the appropriate physical conditions are required in order to maintain the ideal physiological state of the cells. In this study, the changes in lipid chain order during cold shock in the bacterium Bacillus subtilis were studied by means of the quantification of Generalized Polarization (GP) using the fluorescent probe di-4-ANEPPDHQ in vivo and the expression levels of the desaturase [delta]-5 des (as GFP fluorescence) were measured to determine its role as the primary response mechanism to restore membrane fluidity when temperaure is lowered. The data obtained from the GP quantification presented high levels of noise, but a recovery in membrane fluidity was discernible. This result appeared approximately at the time when the GFP levels peaked, showing that the lipid chain modification might be a consequence of [delta]-5 des activity. Additionally, the fusion of the [delta]-5 des promoter with the Enhanced Cyan Fluorescent Protein (ECFP) coding sequence modified with an LVA tail for rapid degradation of the protein was cloned in a vector that enables ectopic integration of the sequence in the chromosome...
publishDate 2014
dc.date.issued.spa.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2022-09-30T14:43:36Z
dc.date.available.none.fl_str_mv 2022-09-30T14:43:36Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
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status_str acceptedVersion
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dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
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672014-1001
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.license.spa.fl_str_mv Atribución-CompartirIgual 4.0 Internacional
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rights_invalid_str_mv Atribución-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 11 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Biología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Ciencias Biológicas
institution Universidad de los Andes
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