Producción de diferentes tipos de carbón activado a partir de cáscara de coco para la inmobilización de bacterias gram negativas
In the present work the research was carried out in the immobilization of gram negative bacteria in activated carbon produced from coconut shell, for the later biogas purification. First, different activated carbon preparations are made using four chemical impregnation agents, two acidic agents (CH3...
- Autores:
-
Ortiz León, Juan Camilo
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2017
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/61528
- Acceso en línea:
- http://hdl.handle.net/1992/61528
- Palabra clave:
- Aprovechamiento de residuos
Bacterias gramnegativas
Carbón activado
Carbón
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
Summary: | In the present work the research was carried out in the immobilization of gram negative bacteria in activated carbon produced from coconut shell, for the later biogas purification. First, different activated carbon preparations are made using four chemical impregnation agents, two acidic agents (CH3COOH and H2SO2) and two basic agents (KOH and NaOH). Then, using bacteria corresponding to the bacterial consortium Odorcarp 5700, the culture was carried out and these bacteria were immobilized in the produced carbons. Finally, a bacterial count was performed on the produced carbons, in order to determine which carbon preparation contains the most immobilized bacteria. The carbon was produced with a pre-treatment, which consisted of pre-impregnation heating with the chosen chemical agents, in an oven at 120§C. However, the production of activated carbon was also performed without the pre-treatment, in order to evaluate whether the pre-treatment performed has an effect on the response variable (Amount of immobilized bacteria or colony forming units formed in the different activated carbon Processed). It was determined that the type of treatment implemented does not have a significant impact on the response variable, on the other hand the impregnating agent does have a significant influence on the amount of bacteria immobilized in the produced carbons, with acidic agents being the most positively incident on the variable of response. It was obtained that the chemical agent with greater capacity of immobilization of bacteria was the H2SO4 realizing the counting by the Neubauer camera, and the CH3COOH by the count in Petri boxes. Finally, a characterization of the carbons with the greatest immobilization of bacteria was carried out. The chemical characterization was performed by electron microscopy (SEM), infrared spectroscopy (NIR) and thermogravimetric analysis (TGA). |
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