Multiscale-based bioproduct design with vegetable extracts of promising amazonian species

"Using innovative bioprospection techniques not only allows to find biological species that could be used as a primary source for obtaining new products, but also to develop those new products and to design their industrial production. This project proposes a multiscale approach as a rational b...

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Autores:
Cardona Jaramillo, Juliana Erika Cristina
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2018
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/41291
Acceso en línea:
http://hdl.handle.net/1992/41291
Palabra clave:
Bioprospección - Investigaciones
Biotensoactivos - Investigaciones
Agentes tensoactivos - Investigaciones
Escherichia coli - Química - Investigaciones
Emulsiones - Investigaciones
Ingeniería
Rights
openAccess
License
https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdf
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network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.es_CO.fl_str_mv Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
title Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
spellingShingle Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
Bioprospección - Investigaciones
Biotensoactivos - Investigaciones
Agentes tensoactivos - Investigaciones
Escherichia coli - Química - Investigaciones
Emulsiones - Investigaciones
Ingeniería
title_short Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
title_full Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
title_fullStr Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
title_full_unstemmed Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
title_sort Multiscale-based bioproduct design with vegetable extracts of promising amazonian species
dc.creator.fl_str_mv Cardona Jaramillo, Juliana Erika Cristina
dc.contributor.advisor.none.fl_str_mv Achenie, Luke E. K
Álvarez Solano, Óscar Alberto
González Barrios, Andrés Fernando
dc.contributor.author.none.fl_str_mv Cardona Jaramillo, Juliana Erika Cristina
dc.contributor.jury.none.fl_str_mv Gómez Ramírez, Jorge Mario
Choplin, Lionel
dc.subject.armarc.es_CO.fl_str_mv Bioprospección - Investigaciones
Biotensoactivos - Investigaciones
Agentes tensoactivos - Investigaciones
Escherichia coli - Química - Investigaciones
Emulsiones - Investigaciones
topic Bioprospección - Investigaciones
Biotensoactivos - Investigaciones
Agentes tensoactivos - Investigaciones
Escherichia coli - Química - Investigaciones
Emulsiones - Investigaciones
Ingeniería
dc.subject.themes.none.fl_str_mv Ingeniería
description "Using innovative bioprospection techniques not only allows to find biological species that could be used as a primary source for obtaining new products, but also to develop those new products and to design their industrial production. This project proposes a multiscale approach as a rational bioprospection way to design emulsions constituted mainly by natural ingredients. The oil phase was obtained from some fruits of Arecaceae species from Colombiaþs amazon region, while the evaluated surfactants were two outer membrane proteins (OmpA and OmpN) obtained from Escherichia coli culture. This approach allowed us to understand how properties at different scales relate to each other, to evaluate the effect of process and product formulation on the emulsion performance, and finally to gauge the surfactant effectiveness. Emulsion quality is related to its stability that could be evaluated considering its properties at the macroscopic, microscopic and molecular scales. For this reason, our work started with a complete molecular characterization of the raw materials as basis to understand their behavior in the formulated emulsions. First, each oil product composition was analyzed by determining the concentration of the main components. As second step, the biosurfactant molecular behavior, at water - triglycerides interfaces, was evaluated in silico through molecular dynamics (MD), and in vitro. Thus, the MD results rationalized the measured experimental evidence. Finally, we implemented a multiscale approach to design emulsions from a knowledge of the composition of the oil phase and its interaction with the surfactant. Properties of formulated emulsions were analyzed from molecular, microscopic and macroscopic scales."--Tomado del Formato de Documento de Grado.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2020-09-03T09:01:37Z
dc.date.available.none.fl_str_mv 2020-09-03T09:01:37Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/41291
dc.identifier.doi.none.fl_str_mv 10.57784/1992/41291
dc.identifier.pdf.none.fl_str_mv u827221.pdf
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
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dc.publisher.program.es_CO.fl_str_mv Doctorado en Ingeniería
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ingeniería
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Achenie, Luke E. Kbba6ae1e-15e7-40d0-94d4-77d862f4e3cf500Álvarez Solano, Óscar Alberto3a4ed8d6-ada0-491c-bd9c-b21a8b183f27400González Barrios, Andrés Fernando007ff982-0b16-4d2e-b379-19c280935a79400Cardona Jaramillo, Juliana Erika Cristina25811500Gómez Ramírez, Jorge MarioChoplin, Lionel2020-09-03T09:01:37Z2020-09-03T09:01:37Z2018http://hdl.handle.net/1992/4129110.57784/1992/41291u827221.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/"Using innovative bioprospection techniques not only allows to find biological species that could be used as a primary source for obtaining new products, but also to develop those new products and to design their industrial production. This project proposes a multiscale approach as a rational bioprospection way to design emulsions constituted mainly by natural ingredients. The oil phase was obtained from some fruits of Arecaceae species from Colombiaþs amazon region, while the evaluated surfactants were two outer membrane proteins (OmpA and OmpN) obtained from Escherichia coli culture. This approach allowed us to understand how properties at different scales relate to each other, to evaluate the effect of process and product formulation on the emulsion performance, and finally to gauge the surfactant effectiveness. Emulsion quality is related to its stability that could be evaluated considering its properties at the macroscopic, microscopic and molecular scales. For this reason, our work started with a complete molecular characterization of the raw materials as basis to understand their behavior in the formulated emulsions. First, each oil product composition was analyzed by determining the concentration of the main components. As second step, the biosurfactant molecular behavior, at water - triglycerides interfaces, was evaluated in silico through molecular dynamics (MD), and in vitro. Thus, the MD results rationalized the measured experimental evidence. Finally, we implemented a multiscale approach to design emulsions from a knowledge of the composition of the oil phase and its interaction with the surfactant. Properties of formulated emulsions were analyzed from molecular, microscopic and macroscopic scales."--Tomado del Formato de Documento de Grado."El uso de técnicas innovadoras de bioprospección no solo permite encontrar especies biológicas que pueden ser usadas como fuente primaria para obtener nuevos productos, sino también desarrollar estos nuevos productos y diseñar su producción industrial. Este proyecto propone un enfoque multiescala como un camino racional de bioprospección para diseñar emulsiones constituidas principalmente por ingredientes naturales. La fase oleosa fue obtenida de frutos de especies de la familia Arecaceae de la región amazónica colombiana, mientras que los surfactantes evaluados fueron dos proteínas transmembranales (OmpA y OmpN) obtenidas del cultivo de Escherichia coli. Este enfoque nos permitió entender como las propiedades a diferentes escalas se relacionan entre sí, evaluar el efecto del proceso y la formulación del producto en el comportamiento de la emulsión, y finalmente valorar la efectividad del biosurfactante. La calidad de la emulsión está relacionada con su estabilidad y puede ser evaluada considerando sus propiedades a escala molecular, microscópica y macroscópica. Por tal razón, nuestro trabajo inició con una completa caracterización de las materias primas como base para entender su comportamiento en las emulsiones formuladas. Primero, la composición de cada aceite fue evaluada para determinar la concentración de los componentes mayoritarios. Luego, el comportamiento molecular de los biosurfactantes en la interfaz agua - aceite fue evaluada in silico por medio de dinámica molecular, e in vitro por técnicas experimentales. Luego, los resultados de dinámica molecular fueron racionalizados a partir de las mediciones experimentales. Finalmente, implementamos la estrategia multiescala para diseñar emulsiones desde el conocimiento de la composición de la fase oleosa y su interacción con el biosurfactantes. Las propiedades de las emulsiones formuladas también fueron analizadas a nivel molecular, microscópico y macroscópico."--Tomado del Formato de Documento de Grado.Doctor en IngenieríaDoctorado102 hojasapplication/pdfengUniandesDoctorado en IngenieríaFacultad de Ingenieríainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaMultiscale-based bioproduct design with vegetable extracts of promising amazonian speciesTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesishttp://purl.org/coar/resource_type/c_db06http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TDBioprospección - InvestigacionesBiotensoactivos - InvestigacionesAgentes tensoactivos - InvestigacionesEscherichia coli - Química - InvestigacionesEmulsiones - InvestigacionesIngenieríaPublicationORIGINALu827221.pdfapplication/pdf3008270https://repositorio.uniandes.edu.co/bitstreams/771802e9-635f-4bae-b517-d9637ab26409/download21334116a4375f1048da62191804a173MD51TEXTu827221.pdf.txtu827221.pdf.txtExtracted texttext/plain211785https://repositorio.uniandes.edu.co/bitstreams/30ac66f0-c9d2-4f3c-a567-a4c6a8c17b5f/download10e8cf3331aad967445e89f6c4be193aMD54THUMBNAILu827221.pdf.jpgu827221.pdf.jpgIM Thumbnailimage/jpeg9080https://repositorio.uniandes.edu.co/bitstreams/17140b53-8012-4788-913c-93eb0f94f837/download148278c95f27be051e853ddb4b632fecMD551992/41291oai:repositorio.uniandes.edu.co:1992/412912024-08-26 15:21:39.308https://repositorio.uniandes.edu.co/static/pdf/aceptacion_uso_es.pdfopen.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co