Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This...
- Autores:
-
Chavarro Vargas, Luisa Fernanda
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/59490
- Acceso en línea:
- http://hdl.handle.net/1992/59490
- Palabra clave:
- Mycorrhizae
Ectomycorrhizae
ITS2
Tropical Dry Forest
Restoration
Metabarcoding
Microbiología
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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|
dc.title.none.fl_str_mv |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
dc.title.alternative.none.fl_str_mv |
Hongos micorrícicos asociados a Coccoloba uvifera en bosque seco tropical Colombiano: un enfoque de restauración ecológica |
title |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
spellingShingle |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration Mycorrhizae Ectomycorrhizae ITS2 Tropical Dry Forest Restoration Metabarcoding Microbiología |
title_short |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
title_full |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
title_fullStr |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
title_full_unstemmed |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
title_sort |
Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration |
dc.creator.fl_str_mv |
Chavarro Vargas, Luisa Fernanda |
dc.contributor.advisor.none.fl_str_mv |
Madriñan, Santiago Corrales, Adriana |
dc.contributor.author.none.fl_str_mv |
Chavarro Vargas, Luisa Fernanda |
dc.subject.keyword.none.fl_str_mv |
Mycorrhizae Ectomycorrhizae ITS2 Tropical Dry Forest Restoration Metabarcoding |
topic |
Mycorrhizae Ectomycorrhizae ITS2 Tropical Dry Forest Restoration Metabarcoding Microbiología |
dc.subject.themes.es_CO.fl_str_mv |
Microbiología |
description |
The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This study case focuses on Coccoloba uvifera (sea grape), a plant species present in tropical dry forests, which an important floristic element in coastal ecological restoration and commonly propagated in forest nurseries. This species also forms ectomycorrhizal) associations. This research compares the composition and diversity of root-associated fungal communities present in seedlings of C. uvifera growing in natural forest and in a forest nursery with and without natural soil inoculum. To do so the nursery¿s traditional treatment and a native soil modified one were used to grow C. uvifera seedlings and measure them after two and four months of growth. After four months, the entire root system of 32 seedlings was collected for DNA extraction and quantification of mycorrhizal colonization. DNA sequencing was carried out by amplifying the fungal ITS2 region using the Illumina miseq platform. In addition, 19 fruiting bodies of ectomycorrhizal fungi found near the sampled C. uvifera natural populations were collected. Our results showed strong differences in root-associated fungal community composition of seedlings growing in different nursery treatments and natural population. Strikingly, seedling growing in traditional nursery substrates without natural soil inoculum show very little to no colonization of symbiotic fungi in their roots. Preliminary results of seedlings growth rate showed no significant differences in plant between treatments after four months. The characterization of EcM fungal communities associated with of this iconic tropical dry forest tree species allows the improvement of local restauration and conservation efforts. The differences found suggest inoculating seedling could be an effective way of restoring soil microbial communities with plants produced ex-situ for reforestation. |
publishDate |
2022 |
dc.date.accessioned.none.fl_str_mv |
2022-08-02T18:40:31Z |
dc.date.available.none.fl_str_mv |
2022-08-02T18:40:31Z |
dc.date.issued.none.fl_str_mv |
2022-07-29 |
dc.type.none.fl_str_mv |
Trabajo de grado - Pregrado |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_7a1f |
dc.type.content.es_CO.fl_str_mv |
Text |
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http://purl.org/redcol/resource_type/TP |
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http://purl.org/coar/resource_type/c_7a1f |
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acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/59490 |
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instname:Universidad de los Andes |
dc.identifier.reponame.none.fl_str_mv |
reponame:Repositorio Institucional Séneca |
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repourl:https://repositorio.uniandes.edu.co/ |
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dc.language.iso.es_CO.fl_str_mv |
eng |
language |
eng |
dc.relation.references.es_CO.fl_str_mv |
Brundrett, M. C., & Tedersoo, L. (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108-1115. https://doi.org/10.1111/nph.14976 Chagnon, P. L., Bradley, R. L., Maherali, H., & Klironomos, J. N. (2013). A trait-based framework to understand life history of mycorrhizal fungi. Trends in Plant Science, 18(9), 484-491. https://doi.org/10.1016/j.tplants.2013.05.001 Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x Gavito, M. E., Pérez-Castillo, D., González-Monterrubio, C. F., Vieyra-Hernández, T., & Martínez-Trujillo, M. (2008). High compatibility between arbuscular mycorrhizal fungal communities and seedlings of different land use types in a tropical dry ecosystem. Mycorrhiza, 19(1) 47-60. https://doi.org/10.1007/s00572-008-0203-4 González-M, R., García, H., Isaacs, P., Cuadros, H., López-Camacho, R., Rodríguez, N., Pérez, K., Mijares, F., Castaño-Naranjo, A., Jurado, R., Idárraga-Piedrahíta, Á., Rojas, A., Vergara, H., & Pizano, C. (2018). Disentangling the environmental heterogeneity, floristic distinctiveness and current threats of tropical dry forests in Colombia. Environmental Research Letters, 13(4), 045007. https://doi.org/10.1088/1748- 9326/aaad74 Howard, R. A. (1949). The genus Coccoloba in Cuba. Journal of the Arnold Arboretum, 30, 388-424. https://doi.org/10.5962/bhl.part.18052 Koide, R. T., & Dickie, I. A. (2002). Effects of mycorrhizal fungi on plant populations. En S. E. Smith & F. A. Smith (Eds.), Diversity and Integration in Mycorrhizas (pp. 307-317). Springer Netherlands. https://doi.org/10.1007/978-94-017-1284-2_30 Koenemann, D. M., & Burke, J. M. (2020). A Molecular Phylogeny for the Genus Coccoloba (Polygonaceae) with an Assessment of Biogeographic Patterns . Systematic Botany, 45(3), 567-575. https://doi.org/10.1600/036364420x15935294613536 Melo, E. de. (2004). As espécies de Coccoloba P. Browne (Polygonaceae) da Amazônia brasileira. Acta Amazonica, 34(4), 525-551. https://doi.org/10.1590/s0044- 59672004000400006 Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia (pp. 21-22). Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. https://doi.org/10.21068/b001.2015.202 Põlme, S., Bahram, M., Kõljalg, U., & Tedersoo, L. (2017). Biogeography and Specificity of Ectomycorrhizal Fungi of Coccoloba uvifera. 345-359. https://doi.org/10.1007/978-3- 319-56363-3_16 Põlme, S., Abarenkov, K., Henrik Nilsson, R., Lindahl, B. D., Clemmensen, K. E., Kauserud, H., Nguyen, N., Kjøller, R., Bates, S. T., Baldrian, P., Frøslev, T. G., Adojaan, K., Vizzini, A., Suija, A., Pfister, D., Baral, H.-O., Järv, H., Madrid, H., Nordén, J., Tedersoo, L. (2020). FungalTraits: A user-friendly traits database of fungi and fungus-likestramenopiles. Fungal Diversity, 105(1), 1-16. https://doi.org/10.1007/s13225-020-00466- 2 Raymond, A., Diédhiou, A. G., Chaintreuil, C., Bessard, S., Séne, S., Omrane, A., Courtecuisse, R., Sylla, S., Duponnois, R., & Bâ, A. M. (2012). Changes in ectomycorrhizal community structure of Coccoloba uvifera L mature trees and regenerating seedlings at two levels of salini ty. The Mycorrhizal Symbiosis in Mediterranean Environment: Importance in Ecosystem Stability and in Soil Rehabilitation Strategies, 151-167. Séne, S., Avril, R., Chaintreuil, C., Geoffroy, A., Ndiaye, C., Diédhiou, A. G., Sadio, O., Courtecuisse, R., Sylla, S. N., Selosse, M. A., & Bâ, A. (2015). Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles). Mycorrhiza, 25(7), 547-559. https://doi.org/10.1007/s00572-015-0633-8 Séne, S., Selosse, M. A., Forget, M., Lambourdière, J., Cissé, K., Diédhiou, A. G., RiveraOcasio, E., Kodja, H., Kameyama, N., Nara, K., Vincenot, L., Mansot, J. L., Weber, J., Roy, M., Sylla, S. N., & Bâ, A. (2018). A pantropically introduced tree is followed by specific ectomycorrhizal symbionts due to pseudo-vertical transmission. ISME Journal, 12(7), 1806-1816. https://doi.org/10.1038/s41396-018-0088-y Tedersoo, L., Sadam, A., Zambrano, M., Valencia, R., & Bahram, M. (2010). Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot. ISME Journal, 4(4), 465-471. https://doi.org/10.1038/ismej.2009.131 Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N. S., Wijesundera, R., Ruiz, L. V., Vasco-Palacios, A. M., Thu, P. Q., Suija, A., Smith, M. E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., Riit, T., Ratkowsky, D., Pritsch, K., Põldmaa, K., ¿ Abarenkov, K. (2014). Global diversity and geography of soil fungi. Science, 346(6213), 1256688. https://doi.org/10.1126/science.1256688 Vierheilig, H., Coughlan, A. P., Wyss, U., & Piché, Y. (1998). Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi. Applied and Environmental Microbiology, 64(12), 5004-5007. https://doi.org/10.1128/AEM.64.12.5004-5007.1998 |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Madriñan, Santiago13c4e98d-2e77-41e5-9f95-5749c5486a77600Corrales, Adriana26e11298-1e7a-4759-9dd8-94a881566c32600Chavarro Vargas, Luisa Fernanda0b4a66dc-8d79-460f-9db0-36f9e38815a36002022-08-02T18:40:31Z2022-08-02T18:40:31Z2022-07-29http://hdl.handle.net/1992/59490instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This study case focuses on Coccoloba uvifera (sea grape), a plant species present in tropical dry forests, which an important floristic element in coastal ecological restoration and commonly propagated in forest nurseries. This species also forms ectomycorrhizal) associations. This research compares the composition and diversity of root-associated fungal communities present in seedlings of C. uvifera growing in natural forest and in a forest nursery with and without natural soil inoculum. To do so the nursery¿s traditional treatment and a native soil modified one were used to grow C. uvifera seedlings and measure them after two and four months of growth. After four months, the entire root system of 32 seedlings was collected for DNA extraction and quantification of mycorrhizal colonization. DNA sequencing was carried out by amplifying the fungal ITS2 region using the Illumina miseq platform. In addition, 19 fruiting bodies of ectomycorrhizal fungi found near the sampled C. uvifera natural populations were collected. Our results showed strong differences in root-associated fungal community composition of seedlings growing in different nursery treatments and natural population. Strikingly, seedling growing in traditional nursery substrates without natural soil inoculum show very little to no colonization of symbiotic fungi in their roots. Preliminary results of seedlings growth rate showed no significant differences in plant between treatments after four months. The characterization of EcM fungal communities associated with of this iconic tropical dry forest tree species allows the improvement of local restauration and conservation efforts. The differences found suggest inoculating seedling could be an effective way of restoring soil microbial communities with plants produced ex-situ for reforestation.Fundación Jardín Botánico de Cartagena "Guillermo Piñeres"Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 110151, ColombiaMicrobiólogoPregrado19 páginasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasMycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restorationHongos micorrícicos asociados a Coccoloba uvifera en bosque seco tropical Colombiano: un enfoque de restauración ecológicaTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPMycorrhizaeEctomycorrhizaeITS2Tropical Dry ForestRestorationMetabarcodingMicrobiologíaBrundrett, M. C., & Tedersoo, L. (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108-1115. https://doi.org/10.1111/nph.14976Chagnon, P. L., Bradley, R. L., Maherali, H., & Klironomos, J. N. (2013). A trait-based framework to understand life history of mycorrhizal fungi. Trends in Plant Science, 18(9), 484-491. https://doi.org/10.1016/j.tplants.2013.05.001Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.xGavito, M. E., Pérez-Castillo, D., González-Monterrubio, C. F., Vieyra-Hernández, T., & Martínez-Trujillo, M. (2008). High compatibility between arbuscular mycorrhizal fungal communities and seedlings of different land use types in a tropical dry ecosystem. Mycorrhiza, 19(1) 47-60. https://doi.org/10.1007/s00572-008-0203-4González-M, R., García, H., Isaacs, P., Cuadros, H., López-Camacho, R., Rodríguez, N., Pérez, K., Mijares, F., Castaño-Naranjo, A., Jurado, R., Idárraga-Piedrahíta, Á., Rojas, A., Vergara, H., & Pizano, C. (2018). Disentangling the environmental heterogeneity, floristic distinctiveness and current threats of tropical dry forests in Colombia. Environmental Research Letters, 13(4), 045007. https://doi.org/10.1088/1748- 9326/aaad74Howard, R. A. (1949). The genus Coccoloba in Cuba. Journal of the Arnold Arboretum, 30, 388-424. https://doi.org/10.5962/bhl.part.18052 Koide, R. T., & Dickie, I. A. (2002). Effects of mycorrhizal fungi on plant populations. En S. E. Smith & F. A. Smith (Eds.), Diversity and Integration in Mycorrhizas (pp. 307-317). Springer Netherlands. https://doi.org/10.1007/978-94-017-1284-2_30Koenemann, D. M., & Burke, J. M. (2020). A Molecular Phylogeny for the Genus Coccoloba (Polygonaceae) with an Assessment of Biogeographic Patterns . Systematic Botany, 45(3), 567-575. https://doi.org/10.1600/036364420x15935294613536 Melo, E. de. (2004). As espécies de Coccoloba P. Browne (Polygonaceae) da Amazônia brasileira. Acta Amazonica, 34(4), 525-551. https://doi.org/10.1590/s0044- 59672004000400006Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia (pp. 21-22). Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. https://doi.org/10.21068/b001.2015.202Põlme, S., Bahram, M., Kõljalg, U., & Tedersoo, L. (2017). Biogeography and Specificity of Ectomycorrhizal Fungi of Coccoloba uvifera. 345-359. https://doi.org/10.1007/978-3- 319-56363-3_16Põlme, S., Abarenkov, K., Henrik Nilsson, R., Lindahl, B. D., Clemmensen, K. E., Kauserud, H., Nguyen, N., Kjøller, R., Bates, S. T., Baldrian, P., Frøslev, T. G., Adojaan, K., Vizzini, A., Suija, A., Pfister, D., Baral, H.-O., Järv, H., Madrid, H., Nordén, J., Tedersoo, L. (2020). FungalTraits: A user-friendly traits database of fungi and fungus-likestramenopiles. Fungal Diversity, 105(1), 1-16. https://doi.org/10.1007/s13225-020-00466- 2Raymond, A., Diédhiou, A. G., Chaintreuil, C., Bessard, S., Séne, S., Omrane, A., Courtecuisse, R., Sylla, S., Duponnois, R., & Bâ, A. M. (2012). Changes in ectomycorrhizal community structure of Coccoloba uvifera L mature trees and regenerating seedlings at two levels of salini ty. The Mycorrhizal Symbiosis in Mediterranean Environment: Importance in Ecosystem Stability and in Soil Rehabilitation Strategies, 151-167.Séne, S., Avril, R., Chaintreuil, C., Geoffroy, A., Ndiaye, C., Diédhiou, A. G., Sadio, O., Courtecuisse, R., Sylla, S. N., Selosse, M. A., & Bâ, A. (2015). Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles). Mycorrhiza, 25(7), 547-559. https://doi.org/10.1007/s00572-015-0633-8Séne, S., Selosse, M. A., Forget, M., Lambourdière, J., Cissé, K., Diédhiou, A. G., RiveraOcasio, E., Kodja, H., Kameyama, N., Nara, K., Vincenot, L., Mansot, J. L., Weber, J., Roy, M., Sylla, S. N., & Bâ, A. (2018). A pantropically introduced tree is followed by specific ectomycorrhizal symbionts due to pseudo-vertical transmission. ISME Journal, 12(7), 1806-1816. https://doi.org/10.1038/s41396-018-0088-yTedersoo, L., Sadam, A., Zambrano, M., Valencia, R., & Bahram, M. (2010). Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot. ISME Journal, 4(4), 465-471. https://doi.org/10.1038/ismej.2009.131Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N. S., Wijesundera, R., Ruiz, L. V., Vasco-Palacios, A. M., Thu, P. Q., Suija, A., Smith, M. E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., Riit, T., Ratkowsky, D., Pritsch, K., Põldmaa, K., ¿ Abarenkov, K. (2014). Global diversity and geography of soil fungi. Science, 346(6213), 1256688. https://doi.org/10.1126/science.1256688Vierheilig, H., Coughlan, A. P., Wyss, U., & Piché, Y. (1998). Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi. 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