Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration

The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This...

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Autores:
Chavarro Vargas, Luisa Fernanda
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2022
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/59490
Acceso en línea:
http://hdl.handle.net/1992/59490
Palabra clave:
Mycorrhizae
Ectomycorrhizae
ITS2
Tropical Dry Forest
Restoration
Metabarcoding
Microbiología
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
id UNIANDES2_4f6f622014d3970898c2d0c82ca1a1c3
oai_identifier_str oai:repositorio.uniandes.edu.co:1992/59490
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.none.fl_str_mv Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
dc.title.alternative.none.fl_str_mv Hongos micorrícicos asociados a Coccoloba uvifera en bosque seco tropical Colombiano: un enfoque de restauración ecológica
title Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
spellingShingle Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
Mycorrhizae
Ectomycorrhizae
ITS2
Tropical Dry Forest
Restoration
Metabarcoding
Microbiología
title_short Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
title_full Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
title_fullStr Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
title_full_unstemmed Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
title_sort Mycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restoration
dc.creator.fl_str_mv Chavarro Vargas, Luisa Fernanda
dc.contributor.advisor.none.fl_str_mv Madriñan, Santiago
Corrales, Adriana
dc.contributor.author.none.fl_str_mv Chavarro Vargas, Luisa Fernanda
dc.subject.keyword.none.fl_str_mv Mycorrhizae
Ectomycorrhizae
ITS2
Tropical Dry Forest
Restoration
Metabarcoding
topic Mycorrhizae
Ectomycorrhizae
ITS2
Tropical Dry Forest
Restoration
Metabarcoding
Microbiología
dc.subject.themes.es_CO.fl_str_mv Microbiología
description The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This study case focuses on Coccoloba uvifera (sea grape), a plant species present in tropical dry forests, which an important floristic element in coastal ecological restoration and commonly propagated in forest nurseries. This species also forms ectomycorrhizal) associations. This research compares the composition and diversity of root-associated fungal communities present in seedlings of C. uvifera growing in natural forest and in a forest nursery with and without natural soil inoculum. To do so the nursery¿s traditional treatment and a native soil modified one were used to grow C. uvifera seedlings and measure them after two and four months of growth. After four months, the entire root system of 32 seedlings was collected for DNA extraction and quantification of mycorrhizal colonization. DNA sequencing was carried out by amplifying the fungal ITS2 region using the Illumina miseq platform. In addition, 19 fruiting bodies of ectomycorrhizal fungi found near the sampled C. uvifera natural populations were collected. Our results showed strong differences in root-associated fungal community composition of seedlings growing in different nursery treatments and natural population. Strikingly, seedling growing in traditional nursery substrates without natural soil inoculum show very little to no colonization of symbiotic fungi in their roots. Preliminary results of seedlings growth rate showed no significant differences in plant between treatments after four months. The characterization of EcM fungal communities associated with of this iconic tropical dry forest tree species allows the improvement of local restauration and conservation efforts. The differences found suggest inoculating seedling could be an effective way of restoring soil microbial communities with plants produced ex-situ for reforestation.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-02T18:40:31Z
dc.date.available.none.fl_str_mv 2022-08-02T18:40:31Z
dc.date.issued.none.fl_str_mv 2022-07-29
dc.type.none.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.version.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
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dc.identifier.instname.none.fl_str_mv instname:Universidad de los Andes
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dc.language.iso.es_CO.fl_str_mv eng
language eng
dc.relation.references.es_CO.fl_str_mv Brundrett, M. C., & Tedersoo, L. (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108-1115. https://doi.org/10.1111/nph.14976
Chagnon, P. L., Bradley, R. L., Maherali, H., & Klironomos, J. N. (2013). A trait-based framework to understand life history of mycorrhizal fungi. Trends in Plant Science, 18(9), 484-491. https://doi.org/10.1016/j.tplants.2013.05.001
Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Gavito, M. E., Pérez-Castillo, D., González-Monterrubio, C. F., Vieyra-Hernández, T., & Martínez-Trujillo, M. (2008). High compatibility between arbuscular mycorrhizal fungal communities and seedlings of different land use types in a tropical dry ecosystem. Mycorrhiza, 19(1) 47-60. https://doi.org/10.1007/s00572-008-0203-4
González-M, R., García, H., Isaacs, P., Cuadros, H., López-Camacho, R., Rodríguez, N., Pérez, K., Mijares, F., Castaño-Naranjo, A., Jurado, R., Idárraga-Piedrahíta, Á., Rojas, A., Vergara, H., & Pizano, C. (2018). Disentangling the environmental heterogeneity, floristic distinctiveness and current threats of tropical dry forests in Colombia. Environmental Research Letters, 13(4), 045007. https://doi.org/10.1088/1748- 9326/aaad74
Howard, R. A. (1949). The genus Coccoloba in Cuba. Journal of the Arnold Arboretum, 30, 388-424. https://doi.org/10.5962/bhl.part.18052 Koide, R. T., & Dickie, I. A. (2002). Effects of mycorrhizal fungi on plant populations. En S. E. Smith & F. A. Smith (Eds.), Diversity and Integration in Mycorrhizas (pp. 307-317). Springer Netherlands. https://doi.org/10.1007/978-94-017-1284-2_30
Koenemann, D. M., & Burke, J. M. (2020). A Molecular Phylogeny for the Genus Coccoloba (Polygonaceae) with an Assessment of Biogeographic Patterns . Systematic Botany, 45(3), 567-575. https://doi.org/10.1600/036364420x15935294613536 Melo, E. de. (2004). As espécies de Coccoloba P. Browne (Polygonaceae) da Amazônia brasileira. Acta Amazonica, 34(4), 525-551. https://doi.org/10.1590/s0044- 59672004000400006
Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia (pp. 21-22). Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. https://doi.org/10.21068/b001.2015.202
Põlme, S., Bahram, M., Kõljalg, U., & Tedersoo, L. (2017). Biogeography and Specificity of Ectomycorrhizal Fungi of Coccoloba uvifera. 345-359. https://doi.org/10.1007/978-3- 319-56363-3_16
Põlme, S., Abarenkov, K., Henrik Nilsson, R., Lindahl, B. D., Clemmensen, K. E., Kauserud, H., Nguyen, N., Kjøller, R., Bates, S. T., Baldrian, P., Frøslev, T. G., Adojaan, K., Vizzini, A., Suija, A., Pfister, D., Baral, H.-O., Järv, H., Madrid, H., Nordén, J., Tedersoo, L. (2020). FungalTraits: A user-friendly traits database of fungi and fungus-likestramenopiles. Fungal Diversity, 105(1), 1-16. https://doi.org/10.1007/s13225-020-00466- 2
Raymond, A., Diédhiou, A. G., Chaintreuil, C., Bessard, S., Séne, S., Omrane, A., Courtecuisse, R., Sylla, S., Duponnois, R., & Bâ, A. M. (2012). Changes in ectomycorrhizal community structure of Coccoloba uvifera L mature trees and regenerating seedlings at two levels of salini ty. The Mycorrhizal Symbiosis in Mediterranean Environment: Importance in Ecosystem Stability and in Soil Rehabilitation Strategies, 151-167.
Séne, S., Avril, R., Chaintreuil, C., Geoffroy, A., Ndiaye, C., Diédhiou, A. G., Sadio, O., Courtecuisse, R., Sylla, S. N., Selosse, M. A., & Bâ, A. (2015). Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles). Mycorrhiza, 25(7), 547-559. https://doi.org/10.1007/s00572-015-0633-8
Séne, S., Selosse, M. A., Forget, M., Lambourdière, J., Cissé, K., Diédhiou, A. G., RiveraOcasio, E., Kodja, H., Kameyama, N., Nara, K., Vincenot, L., Mansot, J. L., Weber, J., Roy, M., Sylla, S. N., & Bâ, A. (2018). A pantropically introduced tree is followed by specific ectomycorrhizal symbionts due to pseudo-vertical transmission. ISME Journal, 12(7), 1806-1816. https://doi.org/10.1038/s41396-018-0088-y
Tedersoo, L., Sadam, A., Zambrano, M., Valencia, R., & Bahram, M. (2010). Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot. ISME Journal, 4(4), 465-471. https://doi.org/10.1038/ismej.2009.131
Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N. S., Wijesundera, R., Ruiz, L. V., Vasco-Palacios, A. M., Thu, P. Q., Suija, A., Smith, M. E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., Riit, T., Ratkowsky, D., Pritsch, K., Põldmaa, K., ¿ Abarenkov, K. (2014). Global diversity and geography of soil fungi. Science, 346(6213), 1256688. https://doi.org/10.1126/science.1256688
Vierheilig, H., Coughlan, A. P., Wyss, U., & Piché, Y. (1998). Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi. Applied and Environmental Microbiology, 64(12), 5004-5007. https://doi.org/10.1128/AEM.64.12.5004-5007.1998
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spelling Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Madriñan, Santiago13c4e98d-2e77-41e5-9f95-5749c5486a77600Corrales, Adriana26e11298-1e7a-4759-9dd8-94a881566c32600Chavarro Vargas, Luisa Fernanda0b4a66dc-8d79-460f-9db0-36f9e38815a36002022-08-02T18:40:31Z2022-08-02T18:40:31Z2022-07-29http://hdl.handle.net/1992/59490instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/The study of mycorrhizal fungi community composition, diversity, and structure is important to understand mechanisms of local dynamics of native forests. Despite their importance and the strong dependency that plant have on these symbiotic fungi, MF are often overlooked in restoration efforts. This study case focuses on Coccoloba uvifera (sea grape), a plant species present in tropical dry forests, which an important floristic element in coastal ecological restoration and commonly propagated in forest nurseries. This species also forms ectomycorrhizal) associations. This research compares the composition and diversity of root-associated fungal communities present in seedlings of C. uvifera growing in natural forest and in a forest nursery with and without natural soil inoculum. To do so the nursery¿s traditional treatment and a native soil modified one were used to grow C. uvifera seedlings and measure them after two and four months of growth. After four months, the entire root system of 32 seedlings was collected for DNA extraction and quantification of mycorrhizal colonization. DNA sequencing was carried out by amplifying the fungal ITS2 region using the Illumina miseq platform. In addition, 19 fruiting bodies of ectomycorrhizal fungi found near the sampled C. uvifera natural populations were collected. Our results showed strong differences in root-associated fungal community composition of seedlings growing in different nursery treatments and natural population. Strikingly, seedling growing in traditional nursery substrates without natural soil inoculum show very little to no colonization of symbiotic fungi in their roots. Preliminary results of seedlings growth rate showed no significant differences in plant between treatments after four months. The characterization of EcM fungal communities associated with of this iconic tropical dry forest tree species allows the improvement of local restauration and conservation efforts. The differences found suggest inoculating seedling could be an effective way of restoring soil microbial communities with plants produced ex-situ for reforestation.Fundación Jardín Botánico de Cartagena "Guillermo Piñeres"Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 110151, ColombiaMicrobiólogoPregrado19 páginasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasMycorrhizal fungi associated with Coccoloba uvifera in Colombian tropical dry forest: an approach for ecological restorationHongos micorrícicos asociados a Coccoloba uvifera en bosque seco tropical Colombiano: un enfoque de restauración ecológicaTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPMycorrhizaeEctomycorrhizaeITS2Tropical Dry ForestRestorationMetabarcodingMicrobiologíaBrundrett, M. C., & Tedersoo, L. (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108-1115. https://doi.org/10.1111/nph.14976Chagnon, P. L., Bradley, R. L., Maherali, H., & Klironomos, J. N. (2013). A trait-based framework to understand life history of mycorrhizal fungi. Trends in Plant Science, 18(9), 484-491. https://doi.org/10.1016/j.tplants.2013.05.001Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.xGavito, M. E., Pérez-Castillo, D., González-Monterrubio, C. F., Vieyra-Hernández, T., & Martínez-Trujillo, M. (2008). High compatibility between arbuscular mycorrhizal fungal communities and seedlings of different land use types in a tropical dry ecosystem. Mycorrhiza, 19(1) 47-60. https://doi.org/10.1007/s00572-008-0203-4González-M, R., García, H., Isaacs, P., Cuadros, H., López-Camacho, R., Rodríguez, N., Pérez, K., Mijares, F., Castaño-Naranjo, A., Jurado, R., Idárraga-Piedrahíta, Á., Rojas, A., Vergara, H., & Pizano, C. (2018). Disentangling the environmental heterogeneity, floristic distinctiveness and current threats of tropical dry forests in Colombia. Environmental Research Letters, 13(4), 045007. https://doi.org/10.1088/1748- 9326/aaad74Howard, R. A. (1949). The genus Coccoloba in Cuba. Journal of the Arnold Arboretum, 30, 388-424. https://doi.org/10.5962/bhl.part.18052 Koide, R. T., & Dickie, I. A. (2002). Effects of mycorrhizal fungi on plant populations. En S. E. Smith & F. A. Smith (Eds.), Diversity and Integration in Mycorrhizas (pp. 307-317). Springer Netherlands. https://doi.org/10.1007/978-94-017-1284-2_30Koenemann, D. M., & Burke, J. M. (2020). A Molecular Phylogeny for the Genus Coccoloba (Polygonaceae) with an Assessment of Biogeographic Patterns . Systematic Botany, 45(3), 567-575. https://doi.org/10.1600/036364420x15935294613536 Melo, E. de. (2004). As espécies de Coccoloba P. Browne (Polygonaceae) da Amazônia brasileira. Acta Amazonica, 34(4), 525-551. https://doi.org/10.1590/s0044- 59672004000400006Pizano, C., González-M., R., López, R., Jurado, R. D., Cuadros, H., Castaño-Naranjo, A., García, H. (2016). El bosque seco tropical en Colombia. In Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia (pp. 21-22). Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. https://doi.org/10.21068/b001.2015.202Põlme, S., Bahram, M., Kõljalg, U., & Tedersoo, L. (2017). Biogeography and Specificity of Ectomycorrhizal Fungi of Coccoloba uvifera. 345-359. https://doi.org/10.1007/978-3- 319-56363-3_16Põlme, S., Abarenkov, K., Henrik Nilsson, R., Lindahl, B. D., Clemmensen, K. E., Kauserud, H., Nguyen, N., Kjøller, R., Bates, S. T., Baldrian, P., Frøslev, T. G., Adojaan, K., Vizzini, A., Suija, A., Pfister, D., Baral, H.-O., Järv, H., Madrid, H., Nordén, J., Tedersoo, L. (2020). FungalTraits: A user-friendly traits database of fungi and fungus-likestramenopiles. Fungal Diversity, 105(1), 1-16. https://doi.org/10.1007/s13225-020-00466- 2Raymond, A., Diédhiou, A. G., Chaintreuil, C., Bessard, S., Séne, S., Omrane, A., Courtecuisse, R., Sylla, S., Duponnois, R., & Bâ, A. M. (2012). Changes in ectomycorrhizal community structure of Coccoloba uvifera L mature trees and regenerating seedlings at two levels of salini ty. The Mycorrhizal Symbiosis in Mediterranean Environment: Importance in Ecosystem Stability and in Soil Rehabilitation Strategies, 151-167.Séne, S., Avril, R., Chaintreuil, C., Geoffroy, A., Ndiaye, C., Diédhiou, A. G., Sadio, O., Courtecuisse, R., Sylla, S. N., Selosse, M. A., & Bâ, A. (2015). Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles). Mycorrhiza, 25(7), 547-559. https://doi.org/10.1007/s00572-015-0633-8Séne, S., Selosse, M. A., Forget, M., Lambourdière, J., Cissé, K., Diédhiou, A. G., RiveraOcasio, E., Kodja, H., Kameyama, N., Nara, K., Vincenot, L., Mansot, J. L., Weber, J., Roy, M., Sylla, S. N., & Bâ, A. (2018). A pantropically introduced tree is followed by specific ectomycorrhizal symbionts due to pseudo-vertical transmission. ISME Journal, 12(7), 1806-1816. https://doi.org/10.1038/s41396-018-0088-yTedersoo, L., Sadam, A., Zambrano, M., Valencia, R., & Bahram, M. (2010). Low diversity and high host preference of ectomycorrhizal fungi in Western Amazonia, a neotropical biodiversity hotspot. ISME Journal, 4(4), 465-471. https://doi.org/10.1038/ismej.2009.131Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N. S., Wijesundera, R., Ruiz, L. V., Vasco-Palacios, A. M., Thu, P. Q., Suija, A., Smith, M. E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., Riit, T., Ratkowsky, D., Pritsch, K., Põldmaa, K., ¿ Abarenkov, K. (2014). Global diversity and geography of soil fungi. Science, 346(6213), 1256688. https://doi.org/10.1126/science.1256688Vierheilig, H., Coughlan, A. P., Wyss, U., & Piché, Y. (1998). Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi. 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