Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation

More than twenty fires over the last ten years have affected El Noviciado estate located in Cerro Majuy, Cota, Colombia; fires led to soil nutrient lost and infertility. Lysinibacillus sphaericus, a gram-positive, mesophilic and spore-forming bacterium, can be used in soil nutrients enrichment withi...

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Autores:
Aguirre Monroy, Angélica María
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2017
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/39244
Acceso en línea:
http://hdl.handle.net/1992/39244
Palabra clave:
Lysinibacillus sphaericus
Efecto de los incendios en los suelos
Bacterias nitrificantes
Fijación del nitrógeno
Microbiología de suelos
Microbiología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.es_CO.fl_str_mv Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
title Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
spellingShingle Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
Lysinibacillus sphaericus
Efecto de los incendios en los suelos
Bacterias nitrificantes
Fijación del nitrógeno
Microbiología de suelos
Microbiología
title_short Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
title_full Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
title_fullStr Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
title_full_unstemmed Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
title_sort Evaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantation
dc.creator.fl_str_mv Aguirre Monroy, Angélica María
dc.contributor.advisor.none.fl_str_mv Dussán Garzón, Jenny
dc.contributor.author.none.fl_str_mv Aguirre Monroy, Angélica María
dc.subject.keyword.es_CO.fl_str_mv Lysinibacillus sphaericus
Efecto de los incendios en los suelos
Bacterias nitrificantes
Fijación del nitrógeno
Microbiología de suelos
topic Lysinibacillus sphaericus
Efecto de los incendios en los suelos
Bacterias nitrificantes
Fijación del nitrógeno
Microbiología de suelos
Microbiología
dc.subject.themes.none.fl_str_mv Microbiología
description More than twenty fires over the last ten years have affected El Noviciado estate located in Cerro Majuy, Cota, Colombia; fires led to soil nutrient lost and infertility. Lysinibacillus sphaericus, a gram-positive, mesophilic and spore-forming bacterium, can be used in soil nutrients enrichment within re-plantation processes, given its ability to fix nitrogen, nitrify and solubilize phosphorus, increasing soil nutrients that are taken for plant growth. In this study, we evaluated the soil-enrichment potential of L. sphaericus by monitoring the nutrients content of a selected fragment of soil in El Noviciado estate. For this purpose, we added a mixture of L. sphaericus OT4b.31, OT4b.49, CBAM5, III(3)7 and 2362 strains and determined the ammonium, nitrites, nitrates, phosphorus an indoleacetic acid concentrations in soil. Also, we transplanted seedlings of Alnus acuminata sbsp. acuminata, which is a native model plant known for its restoration effect. Results indicated that soil with L. sphaericus added presented higher ammonium, nitrites, nitrates, phosphorus and indoleacetic acid concentrations than control soil. Additionally, the analysis for the presence of a molybdenum transporter gene showed band size products between 400-500 bp., indicating the existence of the nitrogenase traditional complex in all the L. sphaericus strains evaluated.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-06-10T16:07:34Z
dc.date.available.none.fl_str_mv 2020-06-10T16:07:34Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
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dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Séneca
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identifier_str_mv u806805.pdf
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dc.language.iso.es_CO.fl_str_mv eng
language eng
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dc.format.extent.es_CO.fl_str_mv 14 hojas
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dc.publisher.es_CO.fl_str_mv Universidad de los Andes
dc.publisher.program.es_CO.fl_str_mv Microbiología
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ciencias
dc.publisher.department.es_CO.fl_str_mv Departamento de Ciencias Biológicas
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87500Aguirre Monroy, Angélica María08825efc-8325-42e4-bae6-0e07cab0388c5002020-06-10T16:07:34Z2020-06-10T16:07:34Z2017http://hdl.handle.net/1992/39244u806805.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/More than twenty fires over the last ten years have affected El Noviciado estate located in Cerro Majuy, Cota, Colombia; fires led to soil nutrient lost and infertility. Lysinibacillus sphaericus, a gram-positive, mesophilic and spore-forming bacterium, can be used in soil nutrients enrichment within re-plantation processes, given its ability to fix nitrogen, nitrify and solubilize phosphorus, increasing soil nutrients that are taken for plant growth. In this study, we evaluated the soil-enrichment potential of L. sphaericus by monitoring the nutrients content of a selected fragment of soil in El Noviciado estate. For this purpose, we added a mixture of L. sphaericus OT4b.31, OT4b.49, CBAM5, III(3)7 and 2362 strains and determined the ammonium, nitrites, nitrates, phosphorus an indoleacetic acid concentrations in soil. Also, we transplanted seedlings of Alnus acuminata sbsp. acuminata, which is a native model plant known for its restoration effect. Results indicated that soil with L. sphaericus added presented higher ammonium, nitrites, nitrates, phosphorus and indoleacetic acid concentrations than control soil. Additionally, the analysis for the presence of a molybdenum transporter gene showed band size products between 400-500 bp., indicating the existence of the nitrogenase traditional complex in all the L. sphaericus strains evaluated."La Hacienda El Noviciado, ubicada en el cerro Majuy, Cota, Colombia, se ha visto afectada por más de veinte incendios en los últimos diez años, esto causó la pérdida de nutrientes del suelo y su infertilidad. Lysinibacillus sphaericus, una bacteria gram-positiva, mesófila y formadora de espora, puede ser utilizada para el enriquecimiento de los nutrientes del suelo dentro de procesos de re-plantación, dada su habilidad de fijar nitrógeno, nitrificar y solubilizar fósforo, aumentando así los nutrientes en el suelo para que puedan ser tomados para el crecimiento de las plantas. En este estudio, nosotros evaluamos a L. Sphaericus como potenciador de nutrientes en suelo mediante el monitoreo del contenido de nutrientes de un fragmento de suelo seleccionado en la Hacienda El Noviciado. Para este propósito, se adicionó un consorcio de las cepas OT4b.31, OT4b.49, CBAM5, III(3)7 and 2362 de L. Sphaericus y se determinaron las concentraciones de amonio, nitritos, nitratos, fósforo y ácido indolacético en el suelo. Además se transplantron plántulas del árbol modelo nativo Alnus acuminata sbsp acuminata, el cual es conocido por su efecto restaurador. Los resultados indicaron que los suelos con L. Sphaericus presentaron mayores concentraciones de amonio, nitritos, nitratos, fósforo y ácido indolacético que los suelos control. Adicionalmente, el análisis para la presencia de un gen transportados de molibdeno mostró un producto de entre 400 y 500 pb., indicando la posible existencia del complejo tradicional de la nitrogenasa en todas las cepas estudiadas."--Tomado del Formato de Documento de Grado.MicrobiólogoPregrado14 hojasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias Biológicasinstname:Universidad de los Andesreponame:Repositorio Institucional SénecaEvaluation of Lysinibacillus sphaericus as a nutrient enhancer during fire-impacted soil re-plantationTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPLysinibacillus sphaericusEfecto de los incendios en los suelosBacterias nitrificantesFijación del nitrógenoMicrobiología de suelosMicrobiologíaPublicationTEXTu806805.pdf.txtu806805.pdf.txtExtracted texttext/plain22733https://repositorio.uniandes.edu.co/bitstreams/a5096969-c97a-48ff-8665-4da4d1c4476e/download195afc619526ad0c275d5491be310864MD54ORIGINALu806805.pdfapplication/pdf669615https://repositorio.uniandes.edu.co/bitstreams/1d9dcd45-2d0a-48e6-9429-41ba526bd903/downloade64f6cafb87d2b7dcb6b3fb7eb33c79bMD51THUMBNAILu806805.pdf.jpgu806805.pdf.jpgIM Thumbnailimage/jpeg19255https://repositorio.uniandes.edu.co/bitstreams/31cd0ed4-74b2-4e26-b642-23193d7b863c/download78049c2fe7f83d7a5dc86aebe6286dc2MD551992/39244oai:repositorio.uniandes.edu.co:1992/392442023-10-10 19:32:46.932http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co

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