Selection and characterization of active cellulases against pretreated oil palm empty fruit bunch from a native endophytes library

Oil palm industry generates a great number of by-products and solid wastes, which includes the oil palm empty fruit bunch (OPEFB). The OPEFB exposes a high cellulose and hemicelluloses content, while displaying low lignin content. This allows the substrate use, to obtain simple sugars, as well as a carbon source for biofuels production. In this study, cellulolytic potential for pretreated OPEFB saccharification, expressed by endophyte microorganism was investigated. One hundred endophytes were screened on solid agar using CMC as substrate. Those that showed cellulolytic activity were grown on cellulase induction medium with pretreated OPEFB as carbon source. The activity of cellulase three main components and total cellulolytic activity was measured. The screening allowed indentifying 28 endophytes with cellulolytic activity. Based on the enzymatic activity, two endophytes (Penicillium sp. and Aspergillus sp.) with maximum enzyme activities, CMCase, FPase, exoglucanases and Beta-glucosidase, of 0.648-0.512, 0.038-0.045, 1.141-2.989 and 0.0606-0.01 U/ml respectively, were selected and partially purified. The cellulases produced by both endophytes were active at low pH (3) and high temperatures (50-65 ºC) which could be a valuable tool for hydrolysis of cellulose under acidic conditions.

• Autores: Medina Solano, Luis Miguel
• Fecha de publicación: 2011
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: eng
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/11522
• Acceso en línea: http://hdl.handle.net/1992/11522
• Palabra clave: Celulasa - Investigaciones; Ambientes extremos - Investigaciones; Desarrollo sostenible - Aspectos ambientales - Investigaciones; Ingeniería
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/4.0/