Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems
Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia plant phyllosphere microorgani...
- Autores:
-
Díaz Riaño, Jorge Iván
- Tipo de recurso:
- Fecha de publicación:
- 2018
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/34599
- Acceso en línea:
- http://hdl.handle.net/1992/34599
- Palabra clave:
- Radiación ultravioleta - Investigaciones
Deinococcus swuensis - Investigaciones
Deinococcus swuensis - Efecto de la radiación ultravioleta
Espeletia - Investigaciones
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.title.es_CO.fl_str_mv |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
title |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
spellingShingle |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems Radiación ultravioleta - Investigaciones Deinococcus swuensis - Investigaciones Deinococcus swuensis - Efecto de la radiación ultravioleta Espeletia - Investigaciones Biología |
title_short |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
title_full |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
title_fullStr |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
title_full_unstemmed |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
title_sort |
Computational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystems |
dc.creator.fl_str_mv |
Díaz Riaño, Jorge Iván |
dc.contributor.advisor.none.fl_str_mv |
Reyes Muñoz, Alejandro Zambrano Eder, María Mercedes |
dc.contributor.author.none.fl_str_mv |
Díaz Riaño, Jorge Iván |
dc.contributor.jury.none.fl_str_mv |
Duitama Castellanos, Jorge Alexander Chica Pratesi, Claudia |
dc.subject.keyword.es_CO.fl_str_mv |
Radiación ultravioleta - Investigaciones Deinococcus swuensis - Investigaciones Deinococcus swuensis - Efecto de la radiación ultravioleta Espeletia - Investigaciones |
topic |
Radiación ultravioleta - Investigaciones Deinococcus swuensis - Investigaciones Deinococcus swuensis - Efecto de la radiación ultravioleta Espeletia - Investigaciones Biología |
dc.subject.themes.none.fl_str_mv |
Biología |
description |
Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia plant phyllosphere microorganisms to survive UVR and to identify genes related to resistance strategies. A strain of D. swuensis showed high survival rate of 60% after UVR treatment at 800 J/m2 and was used for differential expression analysis using RNA-seq after exposing cells to 400 J/m2 of UVR (whit survival rate >95%). Differentially expressed genes were identified, using a D. swuensis sequenced reference strain, with the R-Bioconductor package NOISeq and compared with other reported resistance strategies for bacteria. Few genes were identified as being overexpressed and these included transcriptional regulators and genes involved in protection against damage by UVR. Non-coding (nc)RNAs were also found to be differentially expressed, some of which have been previously implicated in the response to stress conditions. Domain prediction for some unannotated hypothetical proteins is also proposed. Elucidating UVR resistance strategies is crucial to develop and implement novel strategies against resistant bacteria and gain knowledge about their adaptation capacity to extreme environmental conditions. |
publishDate |
2018 |
dc.date.issued.none.fl_str_mv |
2018 |
dc.date.accessioned.none.fl_str_mv |
2020-06-10T09:13:45Z |
dc.date.available.none.fl_str_mv |
2020-06-10T09:13:45Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/34599 |
dc.identifier.pdf.none.fl_str_mv |
u808101.pdf |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad de los Andes |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repositorio.uniandes.edu.co/ |
url |
http://hdl.handle.net/1992/34599 |
identifier_str_mv |
u808101.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
dc.language.iso.es_CO.fl_str_mv |
eng |
language |
eng |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.extent.es_CO.fl_str_mv |
48 hojas |
dc.format.mimetype.es_CO.fl_str_mv |
application/pdf |
dc.publisher.es_CO.fl_str_mv |
Uniandes |
dc.publisher.program.es_CO.fl_str_mv |
Maestría en Biología Computacional |
dc.publisher.faculty.es_CO.fl_str_mv |
Facultad de Ciencias |
dc.publisher.department.es_CO.fl_str_mv |
Departamento de Biología |
dc.source.es_CO.fl_str_mv |
instname:Universidad de los Andes reponame:Repositorio Institucional Séneca |
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Universidad de los Andes |
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Universidad de los Andes |
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Repositorio Institucional Séneca |
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Repositorio Institucional Séneca |
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spelling |
Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Reyes Muñoz, Alejandro9e4d3f3f-d194-4692-aa8b-4497c83504ba400Zambrano Eder, María Mercedes2864709d-4964-4d01-81c6-f52f482c82d0500Díaz Riaño, Jorge Iváne3217240-7527-4243-b7dc-90807569415b500Duitama Castellanos, Jorge AlexanderChica Pratesi, Claudia2020-06-10T09:13:45Z2020-06-10T09:13:45Z2018http://hdl.handle.net/1992/34599u808101.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia plant phyllosphere microorganisms to survive UVR and to identify genes related to resistance strategies. A strain of D. swuensis showed high survival rate of 60% after UVR treatment at 800 J/m2 and was used for differential expression analysis using RNA-seq after exposing cells to 400 J/m2 of UVR (whit survival rate >95%). Differentially expressed genes were identified, using a D. swuensis sequenced reference strain, with the R-Bioconductor package NOISeq and compared with other reported resistance strategies for bacteria. Few genes were identified as being overexpressed and these included transcriptional regulators and genes involved in protection against damage by UVR. Non-coding (nc)RNAs were also found to be differentially expressed, some of which have been previously implicated in the response to stress conditions. Domain prediction for some unannotated hypothetical proteins is also proposed. Elucidating UVR resistance strategies is crucial to develop and implement novel strategies against resistant bacteria and gain knowledge about their adaptation capacity to extreme environmental conditions.La radiación ultravioleta (UVR) es ampliamente conocida como un factor deletéreo para muchos organismos, esta puede causar daños atacando directamente las biomoléculas o generando la formación indirecta de especies reactivas de oxígeno. El objetivo de este estudio fue analizar la capacidad de supervivencia a UVR de los microorganismos asociados a la filósfera de Espeletia. Una cepa de D. swuensis mostró una tasa de supervivencia de 60% posterior al tratamiento por UVR a 800 J/m2 y fue usada para el análisis de expresión diferencial a través de RNA-seq sobre células expuestas a de UVR 400 J/m2 (con una tasa de supervivencia >95%). Los genes diferencialmente expresados fueron identificados usando como referencia una cepa secuenciada de D. swuensis con el paquete para R-Biocondutor NOISeq y comparándolo con estrategias de resistencia reportadas para bacteria. Se identificaron pocos genes diferencialmente expresados, estos incluyeron reguladores transcripcionaes y genes responsables de la protección contra daño por UVR. Algunos ARNs no codificantes (ncARNs) tuvieron expresión diferencial y han sido relacionados con la respuesta a condiciones de estrés. La predicción de dominios funcionales para algunas proteínas hipotéticas también es sugerida. Dilucidar las estrategias de resistencia a UVR es crucial para desarrollar e implementar nuevas aproximaciones contra las bacterias resistentes mejorando el conocimiento acerca de su capacidad de adaptación a condiciones ambientales extremas.Magíster en Biología ComputacionalMaestría48 hojasapplication/pdfengUniandesMaestría en Biología ComputacionalFacultad de CienciasDepartamento de Biologíainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaComputational search of UV radiation resistance strategies in Deinococcus swuensis, isolated from paramo ecosystemsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMRadiación ultravioleta - InvestigacionesDeinococcus swuensis - InvestigacionesDeinococcus swuensis - Efecto de la radiación ultravioletaEspeletia - InvestigacionesBiologíaPublicationORIGINALu808101.pdfapplication/pdf2758483https://repositorio.uniandes.edu.co/bitstreams/d6b42a98-c499-4724-bac1-f3e280a6d4a2/download5fa26ec21163cead59cb164dd6cbeffcMD51TEXTu808101.pdf.txtu808101.pdf.txtExtracted texttext/plain93436https://repositorio.uniandes.edu.co/bitstreams/b21892a0-d65d-4930-aafb-ef60cfd35fb7/download055bb3a329d1c2413c5c053691889e44MD54THUMBNAILu808101.pdf.jpgu808101.pdf.jpgIM Thumbnailimage/jpeg26180https://repositorio.uniandes.edu.co/bitstreams/77e23330-10fb-45c9-bf0d-e71b5cf374b6/download7142099415b13b1f0f8380cfa33a486fMD551992/34599oai:repositorio.uniandes.edu.co:1992/345992023-10-10 19:23:59.762http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |