Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine

Severe environmental and social problems rise up due to the struggle between power and mining activity. Gold mining is an increasing economic activity in Colombia however, it is also the cause of serious environmental and violence problems in the country. A strain of Lysinibacillus sp., was isolated...

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Autores:
Bustos Barbosa, María Camila
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2017
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/61453
Acceso en línea:
http://hdl.handle.net/1992/61453
Palabra clave:
Absorción
Adsorción
Biodegradación de residuos peligrosos
Lysinibacillus sphaericus
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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repository_id_str
dc.title.spa.fl_str_mv Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
title Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
spellingShingle Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
Absorción
Adsorción
Biodegradación de residuos peligrosos
Lysinibacillus sphaericus
title_short Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
title_full Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
title_fullStr Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
title_full_unstemmed Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
title_sort Biosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mine
dc.creator.fl_str_mv Bustos Barbosa, María Camila
dc.contributor.advisor.none.fl_str_mv Dussán Garzón, Jenny
dc.contributor.author.none.fl_str_mv Bustos Barbosa, María Camila
dc.subject.keyword.spa.fl_str_mv Absorción
Adsorción
Biodegradación de residuos peligrosos
Lysinibacillus sphaericus
topic Absorción
Adsorción
Biodegradación de residuos peligrosos
Lysinibacillus sphaericus
description Severe environmental and social problems rise up due to the struggle between power and mining activity. Gold mining is an increasing economic activity in Colombia however, it is also the cause of serious environmental and violence problems in the country. A strain of Lysinibacillus sp., was isolated from an area of an active alluvial gold mining exploitation at El Bagre, Antioquia. It was evident the biosorption and bioaccumulation capacity of the strain in both living and dead cells. For death biomass the percentage of recovery of gold were 86% and 83% for 60æg/mL and 20æg/mL respectively in nutrient broth; while in minimum salt medium were 40% and 21% for the same concentrations. Similarly, in bioassays with live biomass the percentages of gold concentrations were 79% and 2% for nutrient broth; whereas in minimum salt medium were 25% and 46%. According to this, dead cells show a higher percentage of gold recovery through time in comparison to living cells and the development of them is better in higher concentrations of gold and in nutrient broth. Additionally, the presence of S-layer confirms the capacity of this protein to adsorb metals, in this case gold. This can be a potential alternative for processes in biomining related with the extraction of gold in an environmental sustainable approach.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-09-26T22:21:17Z
dc.date.available.none.fl_str_mv 2022-09-26T22:21:17Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
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dc.format.extent.spa.fl_str_mv 19 hojas
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dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Microbiología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Ciencias Biológicas
institution Universidad de los Andes
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87500Bustos Barbosa, María Camilab4a69ac3-64c5-4e5d-a584-ea771a0b56345002022-09-26T22:21:17Z2022-09-26T22:21:17Z2017http://hdl.handle.net/1992/61453instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/795152-1001Severe environmental and social problems rise up due to the struggle between power and mining activity. Gold mining is an increasing economic activity in Colombia however, it is also the cause of serious environmental and violence problems in the country. A strain of Lysinibacillus sp., was isolated from an area of an active alluvial gold mining exploitation at El Bagre, Antioquia. It was evident the biosorption and bioaccumulation capacity of the strain in both living and dead cells. For death biomass the percentage of recovery of gold were 86% and 83% for 60æg/mL and 20æg/mL respectively in nutrient broth; while in minimum salt medium were 40% and 21% for the same concentrations. Similarly, in bioassays with live biomass the percentages of gold concentrations were 79% and 2% for nutrient broth; whereas in minimum salt medium were 25% and 46%. According to this, dead cells show a higher percentage of gold recovery through time in comparison to living cells and the development of them is better in higher concentrations of gold and in nutrient broth. Additionally, the presence of S-layer confirms the capacity of this protein to adsorb metals, in this case gold. This can be a potential alternative for processes in biomining related with the extraction of gold in an environmental sustainable approach.Grandes problemas ambientales y sociales surgen a raíz de la lucha constante entre el poder y la actividad minera. La minería de oro, es una actividad económica importante para el país; sin embargo, a través de historia también ha sido sinónimo de violencia y daño ambiental. Una cepa de Lysinibacillus sp; fue aislada de un área de explotación de minería aluvial de oro activa en El Bagre, Antioquia. Fue evidente su capacidad de adsorción y absorción tanto en biomasa viva como muerta. Para células muertas el porcentaje de recuperación para las concentraciones de 60æg/mL y 20æg/mL oscilo entre 86-83% para caldo nutritivo; y entre 40-25% para medio mínimo de sales. Por otro lado, para los bioensayos con células vivas en las mismas concentraciones y medios fueron de 79-2% y de 46-25% respectivamente. De acuerdo a lo anterior, es claro que la biomasa muerta mostró un porcentaje de recuperación del metal mayor en altas concentraciones del metal y en el medio caldo nutritivo. Por otro lado, la presencia de la proteína capa-s confirmo la capacidad de bioadsorción del metal por parte de la bacteria. En esta medida, es evidente el potencial en biomineria que tiene la cepa, especialmente en procesos de extracción minera de oro desde una aproximación ambientalmente sostenible.--Tomado del Formato de Documento de Grado.MicrobiólogoPregrado19 hojasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasBiosorption and bioaccumulation of gold by a strain of Lysinibacillus sp. insolated of an alluvial mineTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPAbsorciónAdsorciónBiodegradación de residuos peligrososLysinibacillus sphaericus201123632PublicationTHUMBNAIL12840.pdf.jpg12840.pdf.jpgIM Thumbnailimage/jpeg6560https://repositorio.uniandes.edu.co/bitstreams/9f8f9b6d-5b89-4ba3-9520-e8c5351b62cb/downloadcdb014b5b981d99de55de1644bec053dMD53TEXT12840.pdf.txt12840.pdf.txtExtracted texttext/plain28530https://repositorio.uniandes.edu.co/bitstreams/d0081c22-bfba-4b46-ba61-29a050afd362/download72ea6aac0f1c774acee4b37e28c1d932MD52ORIGINAL12840.pdfapplication/pdf875065https://repositorio.uniandes.edu.co/bitstreams/73f2c302-81f7-4410-87e2-78297c248da7/download1bfc9edb0a448e7db9f6df7d148fdd2aMD511992/61453oai:repositorio.uniandes.edu.co:1992/614532023-10-10 19:57:41.196http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co