Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports
Several outbreaks since 1973 have been observed in Colombian cattle with a presumptive diagnosis of Clostridium botulinum diseases [1]. FDA Bacteriological Analytical Manual protocols were used for the isolation of the two representative clostridia microorganisms in Colombia: C. botulinum and C. per...
- Autores:
-
Ayala Montaño, Stefany
- Tipo de recurso:
- Fecha de publicación:
- 2018
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/35010
- Acceso en línea:
- http://hdl.handle.net/1992/35010
- Palabra clave:
- Clostridium - Investigaciones
Toxinas botulínicas - Investigaciones
Enfermedades en el ganado - Investigaciones - Colombia
Ingeniería
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
| id |
UNIANDES2_366d37b257e2f4f42e2a0eecb2a44e46 |
|---|---|
| oai_identifier_str |
oai:repositorio.uniandes.edu.co:1992/35010 |
| network_acronym_str |
UNIANDES2 |
| network_name_str |
Séneca: repositorio Uniandes |
| repository_id_str |
|
| dc.title.es_CO.fl_str_mv |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| title |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| spellingShingle |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports Clostridium - Investigaciones Toxinas botulínicas - Investigaciones Enfermedades en el ganado - Investigaciones - Colombia Ingeniería |
| title_short |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| title_full |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| title_fullStr |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| title_full_unstemmed |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| title_sort |
Uncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reports |
| dc.creator.fl_str_mv |
Ayala Montaño, Stefany |
| dc.contributor.advisor.none.fl_str_mv |
Cordovez Alvarez, Juan Manuel Renjifo Ibáñez, María Camila |
| dc.contributor.author.none.fl_str_mv |
Ayala Montaño, Stefany |
| dc.contributor.jury.none.fl_str_mv |
Caro Quintero, Alejandro Uzal, Francisco |
| dc.subject.keyword.es_CO.fl_str_mv |
Clostridium - Investigaciones Toxinas botulínicas - Investigaciones Enfermedades en el ganado - Investigaciones - Colombia |
| topic |
Clostridium - Investigaciones Toxinas botulínicas - Investigaciones Enfermedades en el ganado - Investigaciones - Colombia Ingeniería |
| dc.subject.themes.none.fl_str_mv |
Ingeniería |
| description |
Several outbreaks since 1973 have been observed in Colombian cattle with a presumptive diagnosis of Clostridium botulinum diseases [1]. FDA Bacteriological Analytical Manual protocols were used for the isolation of the two representative clostridia microorganisms in Colombia: C. botulinum and C. perfringens. Triosephosphatase isomerase (tpi) housekeeping gene - coupled with RFLP approach was done for isolates identification, and further reference primers were used for toxinotype evaluation of 17 isolates expressing proteins in supernatant. C. perfringens isolates expressing alpha toxin were found in soil samples from Nemocón, Cundinamarca and isolate from Barrancas (Guajira) amplified for reference alpha toxin of C. perfringens and Clostridium septicum. Toxins sequence variability was demonstrated in comparison to public database strains available in NCBI; the design of primers for further molecular characterization of local isolates is suggested, along with phylogenetic analysis based on MLST housekeeping genes. |
| publishDate |
2018 |
| dc.date.issued.none.fl_str_mv |
2018 |
| dc.date.accessioned.none.fl_str_mv |
2020-06-10T09:30:58Z |
| dc.date.available.none.fl_str_mv |
2020-06-10T09:30:58Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/35010 |
| dc.identifier.pdf.none.fl_str_mv |
u821186.pdf |
| dc.identifier.instname.spa.fl_str_mv |
instname:Universidad de los Andes |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
| dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repositorio.uniandes.edu.co/ |
| url |
http://hdl.handle.net/1992/35010 |
| identifier_str_mv |
u821186.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
| dc.language.iso.es_CO.fl_str_mv |
eng |
| language |
eng |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.es_CO.fl_str_mv |
49 hojas |
| dc.format.mimetype.es_CO.fl_str_mv |
application/pdf |
| dc.publisher.es_CO.fl_str_mv |
Uniandes |
| dc.publisher.program.es_CO.fl_str_mv |
Maestría en Ingeniería Biomédica |
| dc.publisher.faculty.es_CO.fl_str_mv |
Facultad de Ingeniería |
| dc.publisher.department.es_CO.fl_str_mv |
Departamento de Ingeniería Biomédica |
| dc.source.es_CO.fl_str_mv |
instname:Universidad de los Andes reponame:Repositorio Institucional Séneca |
| instname_str |
Universidad de los Andes |
| institution |
Universidad de los Andes |
| reponame_str |
Repositorio Institucional Séneca |
| collection |
Repositorio Institucional Séneca |
| bitstream.url.fl_str_mv |
https://repositorio.uniandes.edu.co/bitstreams/55ddb04e-23bf-4652-9196-77f65b52391b/download https://repositorio.uniandes.edu.co/bitstreams/3adafd1d-2227-4a3d-92cd-ac09a3c174d4/download https://repositorio.uniandes.edu.co/bitstreams/f9d1b498-f1d4-473b-a615-59e7f4ac8a46/download |
| bitstream.checksum.fl_str_mv |
796a3fc100da8225687d70aab7a08dcb 7ee6815c2afbe3ee8409c067a944b39e 340022a994d630e87cec8c33805343d4 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio institucional Séneca |
| repository.mail.fl_str_mv |
adminrepositorio@uniandes.edu.co |
| _version_ |
1812134046189223936 |
| spelling |
Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cordovez Alvarez, Juan Manuelbcc44a60-316b-4acc-83ed-7231956ec30f400Renjifo Ibáñez, María Camila76b812e6-2bbb-45f1-b1cf-5158e34303fa500Ayala Montaño, Stefany878e2e36-8c04-4540-9585-ff71d171456b500Caro Quintero, AlejandroUzal, Francisco2020-06-10T09:30:58Z2020-06-10T09:30:58Z2018http://hdl.handle.net/1992/35010u821186.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Several outbreaks since 1973 have been observed in Colombian cattle with a presumptive diagnosis of Clostridium botulinum diseases [1]. FDA Bacteriological Analytical Manual protocols were used for the isolation of the two representative clostridia microorganisms in Colombia: C. botulinum and C. perfringens. Triosephosphatase isomerase (tpi) housekeeping gene - coupled with RFLP approach was done for isolates identification, and further reference primers were used for toxinotype evaluation of 17 isolates expressing proteins in supernatant. C. perfringens isolates expressing alpha toxin were found in soil samples from Nemocón, Cundinamarca and isolate from Barrancas (Guajira) amplified for reference alpha toxin of C. perfringens and Clostridium septicum. Toxins sequence variability was demonstrated in comparison to public database strains available in NCBI; the design of primers for further molecular characterization of local isolates is suggested, along with phylogenetic analysis based on MLST housekeeping genes.Se han observado varios brotes desde 1973 en el ganado bovino colombiano con un diagnóstico presuntivo de enfermedades por Clostridium botulinum [1]. Los protocolos del Manual de Análisis Bacteriológico (BAM) de la FDA se utilizaron para el aislamiento de los dos microorganismos clostridiales más representativos en Colombia: C. botulinum y Clostridium perfringens. El gen tpi (triosfosfatasa isomerasa), junto con la técnica RFLP (Restriction Fragment Length Polymorphism) se utilizó para la identificación de los aislamientos. Se emplearon diferentes primers de referencia para la identificación por toxinotipo de 17 aislamientos que demostraron expresar proteínas asociadas a toxina. De los 67 suelos analizados, se encontraron aislamientos de C. perfringens que expresan alfa toxina provenientes de Nemocón, Cundinamarca y aislamientos de Barrancas (Guajira) que amplifican para la alfa toxina de referencia de C. perfringens y Clostridium septicum. La variabilidad en las secuencias nucleotídicas de toxinas fue demostrada en comparación con cepas de bases de datos públicas disponibles en NCBI; se sugiere el diseño de primers locales que permitan caracterizar molecularmente los aislamientos locales. Así como generar nuevas aproximaciones filogenéticas basadas en los genes altamente conservados para análisis de MLST (Multi Locus Sequence Typing).Magíster en Ingeniería BiomédicaMaestría49 hojasapplication/pdfengUniandesMaestría en Ingeniería BiomédicaFacultad de IngenieríaDepartamento de Ingeniería Biomédicainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaUncommon toxinotypes of pathogenic clostridial strains in Colombia : molecular characterization of soil isolates from regions with Clostridium reportsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMClostridium - InvestigacionesToxinas botulínicas - InvestigacionesEnfermedades en el ganado - Investigaciones - ColombiaIngenieríaPublicationTEXTu821186.pdf.txtu821186.pdf.txtExtracted texttext/plain91494https://repositorio.uniandes.edu.co/bitstreams/55ddb04e-23bf-4652-9196-77f65b52391b/download796a3fc100da8225687d70aab7a08dcbMD54ORIGINALu821186.pdfapplication/pdf2783737https://repositorio.uniandes.edu.co/bitstreams/3adafd1d-2227-4a3d-92cd-ac09a3c174d4/download7ee6815c2afbe3ee8409c067a944b39eMD51THUMBNAILu821186.pdf.jpgu821186.pdf.jpgIM Thumbnailimage/jpeg20608https://repositorio.uniandes.edu.co/bitstreams/f9d1b498-f1d4-473b-a615-59e7f4ac8a46/download340022a994d630e87cec8c33805343d4MD551992/35010oai:repositorio.uniandes.edu.co:1992/350102023-10-10 19:18:40.059http://creativecommons.org/licenses/by-nc-nd/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |