ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation

Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effec...

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Autores:
Zárate Bonilla, Lina Johana
Tipo de recurso:
Fecha de publicación:
2012
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/11859
Acceso en línea:
http://hdl.handle.net/1992/11859
Palabra clave:
Klebsiella pneumoniae - Investigaciones
Infecciones nosocomiales - Investigaciones
Biofilms - Investigaciones
Biología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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repository_id_str
dc.title.es_CO.fl_str_mv ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
title ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
spellingShingle ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
Klebsiella pneumoniae - Investigaciones
Infecciones nosocomiales - Investigaciones
Biofilms - Investigaciones
Biología
title_short ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
title_full ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
title_fullStr ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
title_full_unstemmed ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
title_sort ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
dc.creator.fl_str_mv Zárate Bonilla, Lina Johana
dc.contributor.advisor.none.fl_str_mv Zambrano Eder, María Mercedes
Vlamalis, Hera
Restrepo Restrepo, Silvia
dc.contributor.author.none.fl_str_mv Zárate Bonilla, Lina Johana
dc.contributor.jury.none.fl_str_mv Rodríguez Bocanegra, María Ximena
Dussán Garzón, Jenny
dc.subject.keyword.es_CO.fl_str_mv Klebsiella pneumoniae - Investigaciones
Infecciones nosocomiales - Investigaciones
Biofilms - Investigaciones
topic Klebsiella pneumoniae - Investigaciones
Infecciones nosocomiales - Investigaciones
Biofilms - Investigaciones
Biología
dc.subject.themes.none.fl_str_mv Biología
description Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effective treatment and control. In this study, we analyzed the role of the ycgR gene involved in biofilm formation using the clinical strain K. pneumoniae LM21. The ycgR gene was found to be part of an operon together with the luxR-like gene. Deletion of ycgR and complementation analysis with the intact gene indicated that it was responsible for the observed phenotype. However, complementation with both ycgR and luxR increased biofilms with respect to the wild type strain, and even in a ycgR mutant background, suggesting that luxR might also play a role in biofilm formation. The YcgR protein contained a PilZ domain predicted to bind to the c-di-GMP second messenger, while LuxR had a DNA binding domain and was predicted to be a novel type of transcriptional regulator. Using fluorescence microscopy we found that the ycgR mutation affected adherence at early hours of biofilm formation and cell to cell adherence, since a co-incubation with the wild type strain did not promote integration into the formed biofilm. Electron microscopy showed that the ycgR mutant was nonfimbriated in aerobic conditions, even though gene expression of the fimbiral component mrkA gene was still evident. These results show that ycgR is involved in fimbriae production that in turn affects biofilm formation in K. pneumoniae. Additional work is needed to address the specific role of LuxR, its interaction with YcgR and role in biofilm formation under different incubation conditions.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2018-09-28T08:18:29Z
dc.date.available.none.fl_str_mv 2018-09-28T08:18:29Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/11859
dc.identifier.pdf.none.fl_str_mv u627507.pdf
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Séneca
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url http://hdl.handle.net/1992/11859
identifier_str_mv u627507.pdf
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dc.language.iso.es_CO.fl_str_mv eng
language eng
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dc.format.extent.es_CO.fl_str_mv 23 hojas
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dc.publisher.es_CO.fl_str_mv Uniandes
dc.publisher.program.es_CO.fl_str_mv Maestría en Ciencias Biológicas
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ciencias
dc.publisher.department.es_CO.fl_str_mv Departamento de Biología
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Zambrano Eder, María Mercedes2864709d-4964-4d01-81c6-f52f482c82d0600Vlamalis, HeraRestrepo Restrepo, Silviavirtual::5609-1Zárate Bonilla, Lina Johana13d20b2c-b662-4747-98d5-4c8067ff94cc600Rodríguez Bocanegra, María XimenaDussán Garzón, Jenny2018-09-28T08:18:29Z2018-09-28T08:18:29Z2012http://hdl.handle.net/1992/11859u627507.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effective treatment and control. In this study, we analyzed the role of the ycgR gene involved in biofilm formation using the clinical strain K. pneumoniae LM21. The ycgR gene was found to be part of an operon together with the luxR-like gene. Deletion of ycgR and complementation analysis with the intact gene indicated that it was responsible for the observed phenotype. However, complementation with both ycgR and luxR increased biofilms with respect to the wild type strain, and even in a ycgR mutant background, suggesting that luxR might also play a role in biofilm formation. The YcgR protein contained a PilZ domain predicted to bind to the c-di-GMP second messenger, while LuxR had a DNA binding domain and was predicted to be a novel type of transcriptional regulator. Using fluorescence microscopy we found that the ycgR mutation affected adherence at early hours of biofilm formation and cell to cell adherence, since a co-incubation with the wild type strain did not promote integration into the formed biofilm. Electron microscopy showed that the ycgR mutant was nonfimbriated in aerobic conditions, even though gene expression of the fimbiral component mrkA gene was still evident. These results show that ycgR is involved in fimbriae production that in turn affects biofilm formation in K. pneumoniae. Additional work is needed to address the specific role of LuxR, its interaction with YcgR and role in biofilm formation under different incubation conditions.Magíster en Ciencias BiológicasMaestría23 hojasapplication/pdfengUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Biologíainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaycgR and luxR genes are involved in Klebsiella pneumonia biofilm formationTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMKlebsiella pneumoniae - InvestigacionesInfecciones nosocomiales - InvestigacionesBiofilms - InvestigacionesBiologíaPublicationhttps://scholar.google.es/citations?user=7_dVIeAAAAAJvirtual::5609-10000-0001-9016-1040virtual::5609-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000468800virtual::5609-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::5609-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::5609-1THUMBNAILu627507.pdf.jpgu627507.pdf.jpgIM Thumbnailimage/jpeg25490https://repositorio.uniandes.edu.co/bitstreams/a087edf5-e903-46bb-b036-0218dfe73c46/download81790bbcbacc848db86f8910a9d5d08cMD55TEXTu627507.pdf.txtu627507.pdf.txtExtracted texttext/plain52721https://repositorio.uniandes.edu.co/bitstreams/8f362060-e64b-4870-83ac-1555314568ac/download23cd9a23f4c6e67f328d04844dd983c2MD54ORIGINALu627507.pdfapplication/pdf1961524https://repositorio.uniandes.edu.co/bitstreams/9721735c-41a1-4dbd-82c6-5e4d28d4eee6/downloade8d24f0cb7f33df07ac477beebb60bc7MD511992/11859oai:repositorio.uniandes.edu.co:1992/118592024-03-13 12:59:00.103http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co