ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation
Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effec...
- Autores:
-
Zárate Bonilla, Lina Johana
- Tipo de recurso:
- Fecha de publicación:
- 2012
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/11859
- Acceso en línea:
- http://hdl.handle.net/1992/11859
- Palabra clave:
- Klebsiella pneumoniae - Investigaciones
Infecciones nosocomiales - Investigaciones
Biofilms - Investigaciones
Biología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
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oai:repositorio.uniandes.edu.co:1992/11859 |
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UNIANDES2 |
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Séneca: repositorio Uniandes |
repository_id_str |
|
dc.title.es_CO.fl_str_mv |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
title |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
spellingShingle |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation Klebsiella pneumoniae - Investigaciones Infecciones nosocomiales - Investigaciones Biofilms - Investigaciones Biología |
title_short |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
title_full |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
title_fullStr |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
title_full_unstemmed |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
title_sort |
ycgR and luxR genes are involved in Klebsiella pneumonia biofilm formation |
dc.creator.fl_str_mv |
Zárate Bonilla, Lina Johana |
dc.contributor.advisor.none.fl_str_mv |
Zambrano Eder, María Mercedes Vlamalis, Hera Restrepo Restrepo, Silvia |
dc.contributor.author.none.fl_str_mv |
Zárate Bonilla, Lina Johana |
dc.contributor.jury.none.fl_str_mv |
Rodríguez Bocanegra, María Ximena Dussán Garzón, Jenny |
dc.subject.keyword.es_CO.fl_str_mv |
Klebsiella pneumoniae - Investigaciones Infecciones nosocomiales - Investigaciones Biofilms - Investigaciones |
topic |
Klebsiella pneumoniae - Investigaciones Infecciones nosocomiales - Investigaciones Biofilms - Investigaciones Biología |
dc.subject.themes.none.fl_str_mv |
Biología |
description |
Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effective treatment and control. In this study, we analyzed the role of the ycgR gene involved in biofilm formation using the clinical strain K. pneumoniae LM21. The ycgR gene was found to be part of an operon together with the luxR-like gene. Deletion of ycgR and complementation analysis with the intact gene indicated that it was responsible for the observed phenotype. However, complementation with both ycgR and luxR increased biofilms with respect to the wild type strain, and even in a ycgR mutant background, suggesting that luxR might also play a role in biofilm formation. The YcgR protein contained a PilZ domain predicted to bind to the c-di-GMP second messenger, while LuxR had a DNA binding domain and was predicted to be a novel type of transcriptional regulator. Using fluorescence microscopy we found that the ycgR mutation affected adherence at early hours of biofilm formation and cell to cell adherence, since a co-incubation with the wild type strain did not promote integration into the formed biofilm. Electron microscopy showed that the ycgR mutant was nonfimbriated in aerobic conditions, even though gene expression of the fimbiral component mrkA gene was still evident. These results show that ycgR is involved in fimbriae production that in turn affects biofilm formation in K. pneumoniae. Additional work is needed to address the specific role of LuxR, its interaction with YcgR and role in biofilm formation under different incubation conditions. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2018-09-28T08:18:29Z |
dc.date.available.none.fl_str_mv |
2018-09-28T08:18:29Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/1992/11859 |
dc.identifier.pdf.none.fl_str_mv |
u627507.pdf |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad de los Andes |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Séneca |
dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repositorio.uniandes.edu.co/ |
url |
http://hdl.handle.net/1992/11859 |
identifier_str_mv |
u627507.pdf instname:Universidad de los Andes reponame:Repositorio Institucional Séneca repourl:https://repositorio.uniandes.edu.co/ |
dc.language.iso.es_CO.fl_str_mv |
eng |
language |
eng |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.extent.es_CO.fl_str_mv |
23 hojas |
dc.format.mimetype.es_CO.fl_str_mv |
application/pdf |
dc.publisher.es_CO.fl_str_mv |
Uniandes |
dc.publisher.program.es_CO.fl_str_mv |
Maestría en Ciencias Biológicas |
dc.publisher.faculty.es_CO.fl_str_mv |
Facultad de Ciencias |
dc.publisher.department.es_CO.fl_str_mv |
Departamento de Biología |
dc.source.es_CO.fl_str_mv |
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Universidad de los Andes |
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Repositorio Institucional Séneca |
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Repositorio Institucional Séneca |
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spelling |
Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Zambrano Eder, María Mercedes2864709d-4964-4d01-81c6-f52f482c82d0600Vlamalis, HeraRestrepo Restrepo, Silviavirtual::5609-1Zárate Bonilla, Lina Johana13d20b2c-b662-4747-98d5-4c8067ff94cc600Rodríguez Bocanegra, María XimenaDussán Garzón, Jenny2018-09-28T08:18:29Z2018-09-28T08:18:29Z2012http://hdl.handle.net/1992/11859u627507.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that causes different nosocomial infections. Understanding the mechanisms involved in its ability to form biofilms and persist in different environments, can provide useful information in order to propose strategies for effective treatment and control. In this study, we analyzed the role of the ycgR gene involved in biofilm formation using the clinical strain K. pneumoniae LM21. The ycgR gene was found to be part of an operon together with the luxR-like gene. Deletion of ycgR and complementation analysis with the intact gene indicated that it was responsible for the observed phenotype. However, complementation with both ycgR and luxR increased biofilms with respect to the wild type strain, and even in a ycgR mutant background, suggesting that luxR might also play a role in biofilm formation. The YcgR protein contained a PilZ domain predicted to bind to the c-di-GMP second messenger, while LuxR had a DNA binding domain and was predicted to be a novel type of transcriptional regulator. Using fluorescence microscopy we found that the ycgR mutation affected adherence at early hours of biofilm formation and cell to cell adherence, since a co-incubation with the wild type strain did not promote integration into the formed biofilm. Electron microscopy showed that the ycgR mutant was nonfimbriated in aerobic conditions, even though gene expression of the fimbiral component mrkA gene was still evident. These results show that ycgR is involved in fimbriae production that in turn affects biofilm formation in K. pneumoniae. Additional work is needed to address the specific role of LuxR, its interaction with YcgR and role in biofilm formation under different incubation conditions.Magíster en Ciencias BiológicasMaestría23 hojasapplication/pdfengUniandesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Biologíainstname:Universidad de los Andesreponame:Repositorio Institucional SénecaycgR and luxR genes are involved in Klebsiella pneumonia biofilm formationTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TMKlebsiella pneumoniae - InvestigacionesInfecciones nosocomiales - InvestigacionesBiofilms - InvestigacionesBiologíaPublicationhttps://scholar.google.es/citations?user=7_dVIeAAAAAJvirtual::5609-10000-0001-9016-1040virtual::5609-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000468800virtual::5609-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::5609-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::5609-1THUMBNAILu627507.pdf.jpgu627507.pdf.jpgIM Thumbnailimage/jpeg25490https://repositorio.uniandes.edu.co/bitstreams/a087edf5-e903-46bb-b036-0218dfe73c46/download81790bbcbacc848db86f8910a9d5d08cMD55TEXTu627507.pdf.txtu627507.pdf.txtExtracted texttext/plain52721https://repositorio.uniandes.edu.co/bitstreams/8f362060-e64b-4870-83ac-1555314568ac/download23cd9a23f4c6e67f328d04844dd983c2MD54ORIGINALu627507.pdfapplication/pdf1961524https://repositorio.uniandes.edu.co/bitstreams/9721735c-41a1-4dbd-82c6-5e4d28d4eee6/downloade8d24f0cb7f33df07ac477beebb60bc7MD511992/11859oai:repositorio.uniandes.edu.co:1992/118592024-03-13 12:59:00.103http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co |