Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca

The tiger mosquito, a significant public health threat, is vector for numerous diseases, including Dengue and Yellow fever. Aedes albopictus, a species known to cause disease, has been reported in Colombia since 1998. This paper delves into the diverse efforts to control the spread of this insect, w...

Full description

Autores:
Caicedo Murgueitio, Nicolás
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2024
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/74390
Acceso en línea:
https://hdl.handle.net/1992/74390
Palabra clave:
Lysinibacillus sphaericus
Biological control
Mosquito toxic proteins
Aedes albopictus
Microbiología
Rights
openAccess
License
Attribution 4.0 International
id UNIANDES2_2d375682c8bc35bcfdb00abf7c0ff8fd
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network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.eng.fl_str_mv Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
title Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
spellingShingle Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
Lysinibacillus sphaericus
Biological control
Mosquito toxic proteins
Aedes albopictus
Microbiología
title_short Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
title_full Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
title_fullStr Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
title_full_unstemmed Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
title_sort Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, Cundinamarca
dc.creator.fl_str_mv Caicedo Murgueitio, Nicolás
dc.contributor.advisor.none.fl_str_mv González Rosas, Camila
dc.contributor.author.none.fl_str_mv Caicedo Murgueitio, Nicolás
dc.contributor.other.none.fl_str_mv Carrasquilla Ferro, María Cristina
Dussan Garzón, Jenny
dc.contributor.researchgroup.none.fl_str_mv Facultad de Ciencias::Cimpat. Centro de Investigaciones en Microbiología y Parasitologia Tropical
dc.subject.keyword.eng.fl_str_mv Lysinibacillus sphaericus
Biological control
Mosquito toxic proteins
Aedes albopictus
topic Lysinibacillus sphaericus
Biological control
Mosquito toxic proteins
Aedes albopictus
Microbiología
dc.subject.themes.spa.fl_str_mv Microbiología
description The tiger mosquito, a significant public health threat, is vector for numerous diseases, including Dengue and Yellow fever. Aedes albopictus, a species known to cause disease, has been reported in Colombia since 1998. This paper delves into the diverse efforts to control the spread of this insect, with a specific focus on the potential for biological control. Notably, it has been reported that Lysinibacillus sphaericus can eliminate Aedes aegypti species using Mtx1, Mtx2 and Mtx3 mosquito toxic proteins. This study aims to determine the LC50 of L. sphaericus strains III(3)7 and 2362 against Ae. albopictus and to evaluate the mosquitocidal properties of Mtx1, Mtx2 and Mtx3 proteins through transformed E.coli with the individual proteins.
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-06-26T12:43:15Z
dc.date.available.none.fl_str_mv 2024-06-26T12:43:15Z
dc.date.issued.none.fl_str_mv 2024-06-25
dc.type.none.fl_str_mv Trabajo de grado - Pregrado
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
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dc.identifier.instname.none.fl_str_mv instname:Universidad de los Andes
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url https://hdl.handle.net/1992/74390
identifier_str_mv instname:Universidad de los Andes
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dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.references.none.fl_str_mv Velez ID, Quiñones ML, Suarez M, Olano V, Murcia LM, Correa E, et al. Presencia de Aedes albopictus en Leticia, Amazonas, Colombia. Biomédica. 1998;18:192–198.
Gratz, N. G. (2004). Critical review of the vector status of Aedes albopictus. Medical and Veterinary Entomology, 18(3), 215–227.
Potential geographic distribution of the tiger mosquito Aedes albopictus (Skuse, 1894) (Diptera: Culicidae) in current and future conditions for Colombia. PLOSNeglected Tropical Diseases 2021, 15, e0008212.
Vontas, J.; Kioulos, E.; Pavlidi, N.; Morou, E.; della Torre, A.; Ranson, H. Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti. Pesticide Biochemistry and Physiology 2012, 104, 126–131.
Stenberg, J.A.; Sundh, I.; Becher, P.G.; Björkman, C.; Dubey, M.; Egan, P.A.; Friberg, H.; Gil, J.F.; Jensen, D.F.; Jonsson, M.; et al. When is it biological control? A framework of definitions, mechanisms, and classifications. Journal of Pest Science 2021, 94, 665–676.
Rojas-Pinzón, P.A.; Silva-Fernández, J.J.; Dussán, J. Laboratory and simulated-field bioassays for assessing mixed cultures of Lysinibacillus sphaericus against Aedes aegypti (Diptera: Culicidae) larvae resistant to temephos. Applied Entomology and Zoology 2018, 53, 183–191.
Opota, O.; Charles, J.F.; Warot, S.; Pauron, D.; Darboux, I. Identification and characterization of the receptor for the Bacillussphaericus binary toxin in the malaria vector mosquito, Anopheles gambiae. Comparative Biochemistry and Physiology Part B: 134 Biochemistry and Molecular Biology 2008, 149, 419–427.
Tangsongcharoen, C.; Chomanee, N.; Promdonkoy, B.; Boonserm, P. Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae. Journal of Invertebrate Pathology 2015, 128, 57–63.
Ferreira, L.M.; Romão, T.P.; Pompílio de-Melo-Neto, O.; Silva-Filha, M.H.N.L. The orthologue to the Cpm1/Cqm1 receptor in Aedes aegypti is expressed as a midgut GPI-anchored -glucosidase, which does not bind to the insecticidal inary toxin. Insect Biochemistry and Molecular Biology 2010, 40, 604–610. https://doi.org/https://doi.org/10.1016/j.ibmb.2010.05.007.
Berry, C. The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology 2012, 109, 1–10.
Dániel-Gómez, M.; Dussán, J. A Novel Bacterial-Mediated Method for the Differentiation of Aedes spp. Larvae in Mixed-Nursery Assays and Its Application in the Determination of Vegetative Lysinibacillus sphaericus Biocontrol Efficiency in Invasive Vector Eradication. Applied Sciences 2021, 11, 3970.
Teo, C.; Lim, P.; Voon, K.; Mak, J. Detection of dengue viruses and Wolbachia in Aedes aegypti and Aedes albopictus larvae from four urban localities in Kuala Lumpur, Malaysia. Tropical biomedicine 2017, 34, 583–597.
Dussan, J.; Lucia, L.; Andrade, D.; Vanegas, S. Caracterización fisiológica y genética de cepas nativas de Bacillus sphaericus. Revista Colombiana de Biotecnología 2002, 4, 89–99.
Weiser, J. A mosquito-virulent Bacillus sphaericus in adult Simulium damnosum from Northern Nigeria. Zentralblatt für Mikrobiologie 1984, 139, 57–60.
Peng, C.Y.J.; So, T.S.H.; Stage, F.K.; St. John, E.P. The use and interpretation of logistic regression in higher education journals: 1988–1999. Research in higher education 2002, 43, 259–293.
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dc.format.extent.none.fl_str_mv 10 páginas
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dc.publisher.none.fl_str_mv Universidad de los Andes
dc.publisher.program.none.fl_str_mv Microbiología
dc.publisher.faculty.none.fl_str_mv Facultad de Ciencias
dc.publisher.department.none.fl_str_mv Departamento de Ciencias Biológicas
publisher.none.fl_str_mv Universidad de los Andes
institution Universidad de los Andes
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spelling González Rosas, Camilavirtual::18391-1Caicedo Murgueitio, NicolásCarrasquilla Ferro, María CristinaDussan Garzón, JennyFacultad de Ciencias::Cimpat. Centro de Investigaciones en Microbiología y Parasitologia Tropical2024-06-26T12:43:15Z2024-06-26T12:43:15Z2024-06-25https://hdl.handle.net/1992/74390instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/The tiger mosquito, a significant public health threat, is vector for numerous diseases, including Dengue and Yellow fever. Aedes albopictus, a species known to cause disease, has been reported in Colombia since 1998. This paper delves into the diverse efforts to control the spread of this insect, with a specific focus on the potential for biological control. Notably, it has been reported that Lysinibacillus sphaericus can eliminate Aedes aegypti species using Mtx1, Mtx2 and Mtx3 mosquito toxic proteins. This study aims to determine the LC50 of L. sphaericus strains III(3)7 and 2362 against Ae. albopictus and to evaluate the mosquitocidal properties of Mtx1, Mtx2 and Mtx3 proteins through transformed E.coli with the individual proteins.Pregrado10 páginasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias BiológicasAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Efficiency of Lysinibacillus sphaericus in the biological control of Aedes albopictus larvae from La Mesa, CundinamarcaTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPLysinibacillus sphaericusBiological controlMosquito toxic proteinsAedes albopictusMicrobiologíaVelez ID, Quiñones ML, Suarez M, Olano V, Murcia LM, Correa E, et al. Presencia de Aedes albopictus en Leticia, Amazonas, Colombia. Biomédica. 1998;18:192–198.Gratz, N. G. (2004). Critical review of the vector status of Aedes albopictus. Medical and Veterinary Entomology, 18(3), 215–227.Potential geographic distribution of the tiger mosquito Aedes albopictus (Skuse, 1894) (Diptera: Culicidae) in current and future conditions for Colombia. PLOSNeglected Tropical Diseases 2021, 15, e0008212.Vontas, J.; Kioulos, E.; Pavlidi, N.; Morou, E.; della Torre, A.; Ranson, H. Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti. Pesticide Biochemistry and Physiology 2012, 104, 126–131.Stenberg, J.A.; Sundh, I.; Becher, P.G.; Björkman, C.; Dubey, M.; Egan, P.A.; Friberg, H.; Gil, J.F.; Jensen, D.F.; Jonsson, M.; et al. When is it biological control? A framework of definitions, mechanisms, and classifications. Journal of Pest Science 2021, 94, 665–676.Rojas-Pinzón, P.A.; Silva-Fernández, J.J.; Dussán, J. Laboratory and simulated-field bioassays for assessing mixed cultures of Lysinibacillus sphaericus against Aedes aegypti (Diptera: Culicidae) larvae resistant to temephos. Applied Entomology and Zoology 2018, 53, 183–191.Opota, O.; Charles, J.F.; Warot, S.; Pauron, D.; Darboux, I. Identification and characterization of the receptor for the Bacillussphaericus binary toxin in the malaria vector mosquito, Anopheles gambiae. Comparative Biochemistry and Physiology Part B: 134 Biochemistry and Molecular Biology 2008, 149, 419–427.Tangsongcharoen, C.; Chomanee, N.; Promdonkoy, B.; Boonserm, P. Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae. Journal of Invertebrate Pathology 2015, 128, 57–63.Ferreira, L.M.; Romão, T.P.; Pompílio de-Melo-Neto, O.; Silva-Filha, M.H.N.L. The orthologue to the Cpm1/Cqm1 receptor in Aedes aegypti is expressed as a midgut GPI-anchored -glucosidase, which does not bind to the insecticidal inary toxin. Insect Biochemistry and Molecular Biology 2010, 40, 604–610. https://doi.org/https://doi.org/10.1016/j.ibmb.2010.05.007.Berry, C. The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology 2012, 109, 1–10.Dániel-Gómez, M.; Dussán, J. A Novel Bacterial-Mediated Method for the Differentiation of Aedes spp. Larvae in Mixed-Nursery Assays and Its Application in the Determination of Vegetative Lysinibacillus sphaericus Biocontrol Efficiency in Invasive Vector Eradication. Applied Sciences 2021, 11, 3970.Teo, C.; Lim, P.; Voon, K.; Mak, J. Detection of dengue viruses and Wolbachia in Aedes aegypti and Aedes albopictus larvae from four urban localities in Kuala Lumpur, Malaysia. Tropical biomedicine 2017, 34, 583–597.Dussan, J.; Lucia, L.; Andrade, D.; Vanegas, S. Caracterización fisiológica y genética de cepas nativas de Bacillus sphaericus. Revista Colombiana de Biotecnología 2002, 4, 89–99.Weiser, J. A mosquito-virulent Bacillus sphaericus in adult Simulium damnosum from Northern Nigeria. Zentralblatt für Mikrobiologie 1984, 139, 57–60.Peng, C.Y.J.; So, T.S.H.; Stage, F.K.; St. John, E.P. The use and interpretation of logistic regression in higher education journals: 1988–1999. 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