The outer membrane protein A as a model for the study of lipid-protein interactions in complex neuronal membranes

Understanding the dynamics of different proteins with their lipidic environment can provide a great insight regarding the novel uses of this molecules in areas such as drug delivery systems. Here, a model for the study of the protein-lipid interactions of the OmpA protein of the Gram- negative bacteria Escherichia coli with a complex model of a neuronal membrane was studied. Our experimental setup allows the study of the lipid-protein interactions in a way that up to four proteins can be studied simultaneously. Cumulative histograms of specific lipid-protein interaction show differences between periplasmic and transmembrane domains, specifically the periplasmic domains shows high affinity for lipids containing a sugar group. We hope our simulations give insights into the mechanism by which magnetite-coated nanoparticles translocate mammalian membranes.

• Autores: Cifuentes Almanza, Santiago José
• Fecha de publicación: 2019
• Institución: Universidad de los Andes
• Repositorio: Séneca: repositorio Uniandes
• Idioma: eng
• OAI Identifier: oai:repositorio.uniandes.edu.co:1992/43941
• Acceso en línea: http://hdl.handle.net/1992/43941
• Palabra clave: Separación (Tecnología) - Investigaciones; Escherichia coli - Investigaciones; Proteínas bacterianas - Investigaciones; Ingeniería
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/4.0/