Challenge in the diagnosis of Staphylococcus aureus: Discordance between the genotypical and phenotypical profiles
Staphylococcus aureus is a superbug because of its molecular and phenotypical features. That makes this pathogen challenging to track and to establish a successful antibiotic treatment. In this research, 57 S. aureus clinical isolates were characterized by their molecular and phenotypical properties...
- Autores:
-
Mancera García, María Alejandra
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2021
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/55071
- Acceso en línea:
- http://hdl.handle.net/1992/55071
- Palabra clave:
- Aislamientos clínicos
Staphylococcus aureus
Rastreo epidemiológico
SARM
Aislamientos de comunidad
Microbiología
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/4.0/
| Summary: | Staphylococcus aureus is a superbug because of its molecular and phenotypical features. That makes this pathogen challenging to track and to establish a successful antibiotic treatment. In this research, 57 S. aureus clinical isolates were characterized by their molecular and phenotypical properties: The detection of the mecA resistance gene, the pvl virulence gene, Spa typing, and their antibiotic susceptibility profile. S. aureus isolates were classified as methicillin-resistant S. aureus (MRSA), methicillin-sensible S. aureus (MSSA), oxacillin sensible methicillin-resistant S. aureus (OS-MRSA), and borderline oxacillin-resistant S. aureus (BORSA). MRSA correspond to 47% of the total isolates. The presumptive origin of MRSA infection was mainly related to community environments (57% CA-MRSA). We would conclude that community-acquired (CA-MRSA) isolates are replacing healthcare-associated (HA-MRSA). The presence of pvl was detected in almost every clinical group, (e.g., multi-resistant MSSA isolates), and determine this as an ambiguous marker for CA-MRSA. Finally, the spa-typing results allowed us to identify the possible establishment of pandemic clones in Bogotá-Colombia. The characterization of Staphylococcus aureus clinical types is difficult because of transferable mobile genetic elements that harbor remarkable resistance and virulent genes; nonetheless, their accurate identification is necessary to guarantee therapeutic success. |
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