Lysinibacillus sphaericus as species and biotechnological agent : a genomic review

This thesis can be considered the final part of a great project conducted by the CIMIC since 2013 with the first whole genome sequencing of a Colombian strain of Lysinibacillus sphaericus. Such a project was aimed at gaining a comprehensive understanding of L. sphaericus in the light of genomics. Th...

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Autores:
Gómez Garzón, Camilo Andrés
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/61404
Acceso en línea:
http://hdl.handle.net/1992/61404
Palabra clave:
Geobacillus
Lysinibacillus sphaericus
Percepción de quorum (Microbiología)
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.title.spa.fl_str_mv Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
title Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
spellingShingle Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
Geobacillus
Lysinibacillus sphaericus
Percepción de quorum (Microbiología)
title_short Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
title_full Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
title_fullStr Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
title_full_unstemmed Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
title_sort Lysinibacillus sphaericus as species and biotechnological agent : a genomic review
dc.creator.fl_str_mv Gómez Garzón, Camilo Andrés
dc.contributor.advisor.none.fl_str_mv Dussán Garzón, Jenny
dc.contributor.author.none.fl_str_mv Gómez Garzón, Camilo Andrés
dc.contributor.jury.none.fl_str_mv Caro Quintero, Alejandro
Reyes Muñoz, Alejandro
dc.subject.keyword.spa.fl_str_mv Geobacillus
Lysinibacillus sphaericus
Percepción de quorum (Microbiología)
topic Geobacillus
Lysinibacillus sphaericus
Percepción de quorum (Microbiología)
description This thesis can be considered the final part of a great project conducted by the CIMIC since 2013 with the first whole genome sequencing of a Colombian strain of Lysinibacillus sphaericus. Such a project was aimed at gaining a comprehensive understanding of L. sphaericus in the light of genomics. This has been studied in the CIMIC because of its promising use in biocontrol and bioremediation. The first part of the current document (Theoretical framework and Objectives) defines the main concepts involved in the biotechnological potentials of bacteria, focusing on the case of L. sphaericus and its promising potential applications for biocontrol and bioremediation. In addition, the controversy over the species circumscription of L. sphaericus is reviewed. Lastly, this section describes the state of the art and establishes the objectives of the current study. In Chapter 1, the parameters and results of the genome sequencing of the entomopathogenic strains 2362 and OT4b.49 are described. Results from these genome sequencing are compared to the current available L. sphaericus genomes on the NCBI Genome database. In Chapter 2, the species circumscription of L. sphaericus is discussed. A new bacterial species made up of the toxic strains is suggested. This propose is supported by the genomic data obtained in this study and by the experimental evidence previously reported in the scientific literature. In Chapter 3, a metabolic reconstruction of the L. sphaericus toxic strains is presented. This reconstruction was built from the obtained genomic data and offers a guideline for further studies on the biotechnological implementation of L. sphaericus. Finally, the last part of this document shows the Concluding remarks of the current work, including the questions about the L. sphaericus biology that remains unanswered. The Appendices section contains some unpublished results and other supplemental material that bolsters the hypotheses proposed herein.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-09-26T22:18:27Z
dc.date.available.none.fl_str_mv 2022-09-26T22:18:27Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/61404
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
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dc.language.iso.spa.fl_str_mv eng
language eng
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dc.format.extent.spa.fl_str_mv 61 hojas
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dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Maestría en Ciencias Biológicas
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Ciencias Biológicas
institution Universidad de los Andes
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spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dussán Garzón, Jenny9a6bbaf5-51e2-4592-95f4-a9185d737d87500Gómez Garzón, Camilo Andrésaccc2069-d5cd-4601-b572-be4d086b7b61500Caro Quintero, AlejandroReyes Muñoz, Alejandro2022-09-26T22:18:27Z2022-09-26T22:18:27Z2017http://hdl.handle.net/1992/61404instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/795095-1001This thesis can be considered the final part of a great project conducted by the CIMIC since 2013 with the first whole genome sequencing of a Colombian strain of Lysinibacillus sphaericus. Such a project was aimed at gaining a comprehensive understanding of L. sphaericus in the light of genomics. This has been studied in the CIMIC because of its promising use in biocontrol and bioremediation. The first part of the current document (Theoretical framework and Objectives) defines the main concepts involved in the biotechnological potentials of bacteria, focusing on the case of L. sphaericus and its promising potential applications for biocontrol and bioremediation. In addition, the controversy over the species circumscription of L. sphaericus is reviewed. Lastly, this section describes the state of the art and establishes the objectives of the current study. In Chapter 1, the parameters and results of the genome sequencing of the entomopathogenic strains 2362 and OT4b.49 are described. Results from these genome sequencing are compared to the current available L. sphaericus genomes on the NCBI Genome database. In Chapter 2, the species circumscription of L. sphaericus is discussed. A new bacterial species made up of the toxic strains is suggested. This propose is supported by the genomic data obtained in this study and by the experimental evidence previously reported in the scientific literature. In Chapter 3, a metabolic reconstruction of the L. sphaericus toxic strains is presented. This reconstruction was built from the obtained genomic data and offers a guideline for further studies on the biotechnological implementation of L. sphaericus. Finally, the last part of this document shows the Concluding remarks of the current work, including the questions about the L. sphaericus biology that remains unanswered. The Appendices section contains some unpublished results and other supplemental material that bolsters the hypotheses proposed herein.Esta tesis puede considerarse como la contribución final de un gran proyecto de investigación iniciado por el CIMIC en 2013 con la secuenciación del primer genoma de una cepa colombiana de L. sphaericus. Tal proyecto tuvo como objetivo alcanzar un entendimiento de la biología de L. sphaericus como especie y agente en biotecnología desde un perspectiva genómica. La primera parte de este documento define los conceptos claves relacionados con los potenciales biotecnológicos de las bacterias, enfocándose en el caso de L. sphaericus y sus aplicaciones en control biológico y remediación ambiental. Adicionalmente, se describe la controversia taxonómica que actualmente engloba a esta especie y se establecen los objetivos del presente estudio. En el capítulo 1, se detallan los parámetros y resultados de la secuenciación de los genomas de las cepas entomotóxicas 2362 y OT4b.49 en relación a los genomas actualmente disponibles en las bases de datos del NCBI para esta especie. En el capítulo 2, se discute la circunscripción de especie de L. sphaericus. Se sugiere que las cepas tóxicas conforman una especie separada del resto de las cepas, que no poseen una circunscripción clara. Esta hipótesis se soporta con los datos genómicos obtenidos en este estudio así como en evidencia experimental reportada en la literatura. En el capítulo 3, se presenta una reconstrucción metabólica del linaje tóxico de L. sphaericus, donde se resaltan sus potenciales biotecnológicos y se proponen lineamientos para su implementación. Finalmente, en la última parte del documento, se plantean las conclusiones del presente trabajo así como se proponen las preguntas sobre la biología de esta especie que permanecen aún sin respuesta. Se presenta también la sección de apéndices con material suplementario no publicado que sirvió como soporte de las hipótesis planteadas.--Tomado del Formato de Documento de Grado.Magíster en Ciencias BiológicasMaestría61 hojasapplication/pdfengUniversidad de los AndesMaestría en Ciencias BiológicasFacultad de CienciasDepartamento de Ciencias BiológicasLysinibacillus sphaericus as species and biotechnological agent : a genomic reviewTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMGeobacillusLysinibacillus sphaericusPercepción de quorum (Microbiología)200921677PublicationTEXT12274.pdf.txt12274.pdf.txtExtracted texttext/plain102783https://repositorio.uniandes.edu.co/bitstreams/ad5a93c0-c57f-4eb7-a008-20c454fc5cfc/download7ddd4a77fb466ed7e372363cd5c218b6MD52THUMBNAIL12274.pdf.jpg12274.pdf.jpgIM Thumbnailimage/jpeg11300https://repositorio.uniandes.edu.co/bitstreams/d5799d54-3e6a-4231-a3c1-89f512bd1130/download61a0df1845465093caae13b746ccd465MD53ORIGINAL12274.pdfapplication/pdf7305551https://repositorio.uniandes.edu.co/bitstreams/38a32a55-c9b2-4e2b-ae0a-4d5a7fd7f83e/downloade919d7c63e2f4a580a21815710d0f59eMD511992/61404oai:repositorio.uniandes.edu.co:1992/614042023-10-10 17:55:53.814http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co