Validation of the effector profile of Phytophthora betacei

Phytophthora betacei is a new species that was determined through a set of genetic, ecologic and morphological characteristics that make it stand it apart independently from P. infestans. This new species is capable of infecting tree tomato (Solanum betaceum) yet it is not able to infect common pota...

Full description

Autores:
Rivera Ladino, José Miguel
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2017
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/39984
Acceso en línea:
http://hdl.handle.net/1992/39984
Palabra clave:
Phytophthora infestans
Tomate de árbol
Fungicidas sistémicos
Microbiología
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
id UNIANDES2_1bd84df8d1960fc3a2fa967c1cb07bf1
oai_identifier_str oai:repositorio.uniandes.edu.co:1992/39984
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
dc.title.es_CO.fl_str_mv Validation of the effector profile of Phytophthora betacei
title Validation of the effector profile of Phytophthora betacei
spellingShingle Validation of the effector profile of Phytophthora betacei
Phytophthora infestans
Tomate de árbol
Fungicidas sistémicos
Microbiología
title_short Validation of the effector profile of Phytophthora betacei
title_full Validation of the effector profile of Phytophthora betacei
title_fullStr Validation of the effector profile of Phytophthora betacei
title_full_unstemmed Validation of the effector profile of Phytophthora betacei
title_sort Validation of the effector profile of Phytophthora betacei
dc.creator.fl_str_mv Rivera Ladino, José Miguel
dc.contributor.advisor.none.fl_str_mv Restrepo Restrepo, Silvia
Mideros Bastidas, María Fernanda
dc.contributor.author.none.fl_str_mv Rivera Ladino, José Miguel
dc.subject.keyword.es_CO.fl_str_mv Phytophthora infestans
Tomate de árbol
Fungicidas sistémicos
topic Phytophthora infestans
Tomate de árbol
Fungicidas sistémicos
Microbiología
dc.subject.themes.none.fl_str_mv Microbiología
description Phytophthora betacei is a new species that was determined through a set of genetic, ecologic and morphological characteristics that make it stand it apart independently from P. infestans. This new species is capable of infecting tree tomato (Solanum betaceum) yet it is not able to infect common potato species. The effector proteins determine this host-pathogen interaction, RXLR and Crinkler effectors are the most studied in the Phytophthora genus for its degree of conserved regions. A bioinformatic study did predictions of new effectors based on the sequenced fragments of samples of the infecting agent that caused an outbreak in Pasto, Colombia. The objective of this study was to validate some of this predictions and contribute to the genetic profile of this new species. There were two gene candidates that are potentially unique genes for P. betacei. Finally, Pex151803 was validated experimentally as an effector gene
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-06-10T16:43:11Z
dc.date.available.none.fl_str_mv 2020-06-10T16:43:11Z
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TP
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/39984
dc.identifier.pdf.none.fl_str_mv u807563.pdf
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Séneca
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
url http://hdl.handle.net/1992/39984
identifier_str_mv u807563.pdf
instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
repourl:https://repositorio.uniandes.edu.co/
dc.language.iso.es_CO.fl_str_mv eng
language eng
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.es_CO.fl_str_mv 12 hojas
dc.format.mimetype.es_CO.fl_str_mv application/pdf
dc.publisher.es_CO.fl_str_mv Universidad de los Andes
dc.publisher.program.es_CO.fl_str_mv Microbiología
dc.publisher.faculty.es_CO.fl_str_mv Facultad de Ciencias
dc.publisher.department.es_CO.fl_str_mv Departamento de Ciencias Biológicas
dc.source.es_CO.fl_str_mv instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
instname_str Universidad de los Andes
institution Universidad de los Andes
reponame_str Repositorio Institucional Séneca
collection Repositorio Institucional Séneca
bitstream.url.fl_str_mv https://repositorio.uniandes.edu.co/bitstreams/11b89c22-82b6-4547-9c81-3fa221f39d96/download
https://repositorio.uniandes.edu.co/bitstreams/8f9114bb-c405-4a82-aff1-7a1951cb2669/download
https://repositorio.uniandes.edu.co/bitstreams/208b1599-962f-4203-9a5f-f329bf47eaa7/download
bitstream.checksum.fl_str_mv 14ef8e75153bf601e30f935b67afaf1d
763cf2497637f1565f027de7496e578e
2dad0c9ef67c0a769a2591c71b75ff21
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio institucional Séneca
repository.mail.fl_str_mv adminrepositorio@uniandes.edu.co
_version_ 1812134011806416896
spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Restrepo Restrepo, Silviavirtual::13351-1Mideros Bastidas, María Fernandavirtual::13352-1Rivera Ladino, José Miguela4279642-03d9-4202-8b13-47ced4da2eb85002020-06-10T16:43:11Z2020-06-10T16:43:11Z2017http://hdl.handle.net/1992/39984u807563.pdfinstname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Phytophthora betacei is a new species that was determined through a set of genetic, ecologic and morphological characteristics that make it stand it apart independently from P. infestans. This new species is capable of infecting tree tomato (Solanum betaceum) yet it is not able to infect common potato species. The effector proteins determine this host-pathogen interaction, RXLR and Crinkler effectors are the most studied in the Phytophthora genus for its degree of conserved regions. A bioinformatic study did predictions of new effectors based on the sequenced fragments of samples of the infecting agent that caused an outbreak in Pasto, Colombia. The objective of this study was to validate some of this predictions and contribute to the genetic profile of this new species. There were two gene candidates that are potentially unique genes for P. betacei. Finally, Pex151803 was validated experimentally as an effector gene"Phytophthora betacei es una nueva especie que se determino a partir de un estudio genetico, morfologico y ecologico que la separan de P. infestans. Esta nueva especie es capaz de infectar tomate de arbol (Solanum betaceum) pero no es capaz de infectar especies de papa. Las efectores son proteinas que determinan estas interacciones de hospedero-parasito. Los RXLR y los Crinklers son los mas estudiados para el genero Phytophthora y a su vez tiene un grado alto de regiones conservadas. A partir de un estudio bioinformatico del perfil de esta nueva especie se elaboraron predicciones sobre nuevos efectores basados en secuenciacion de muestras que causaron una epidemia en Pasto, Colombia. El objetivo de este estudio es validar estar predicciones y contribuir al perfil genetico de esta nueva especie. Hubo dos candidatos que son potencialmente genes unicos para P. betacei. Finalmente, el gen Pex151803 fue validado experimentalmente como un efector."--Tomado del Formato de Documento de GradoMicrobiólogoPregrado12 hojasapplication/pdfengUniversidad de los AndesMicrobiologíaFacultad de CienciasDepartamento de Ciencias Biológicasinstname:Universidad de los Andesreponame:Repositorio Institucional SénecaValidation of the effector profile of Phytophthora betaceiTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/TPPhytophthora infestansTomate de árbolFungicidas sistémicosMicrobiologíaPublicationhttps://scholar.google.es/citations?user=7_dVIeAAAAAJvirtual::13351-10000-0001-9016-1040virtual::13351-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000468800virtual::13351-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000620505virtual::13352-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::13351-1ed23f670-0672-4034-93dc-b7de12470813virtual::13352-1d7d594d1-aae9-471e-be1d-fc6bbcabef5cvirtual::13351-1ed23f670-0672-4034-93dc-b7de12470813virtual::13352-1ORIGINALu807563.pdfapplication/pdf374706https://repositorio.uniandes.edu.co/bitstreams/11b89c22-82b6-4547-9c81-3fa221f39d96/download14ef8e75153bf601e30f935b67afaf1dMD51TEXTu807563.pdf.txtu807563.pdf.txtExtracted texttext/plain15732https://repositorio.uniandes.edu.co/bitstreams/8f9114bb-c405-4a82-aff1-7a1951cb2669/download763cf2497637f1565f027de7496e578eMD54THUMBNAILu807563.pdf.jpgu807563.pdf.jpgIM Thumbnailimage/jpeg5889https://repositorio.uniandes.edu.co/bitstreams/208b1599-962f-4203-9a5f-f329bf47eaa7/download2dad0c9ef67c0a769a2591c71b75ff21MD551992/39984oai:repositorio.uniandes.edu.co:1992/399842024-03-13 14:54:58.153http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co