Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique

"Interest in studies about variability in gene expression has been increasing due to its applications in pharmaceutical, medical and biological sciences. Genetically identical cells can have different phenotypes even when they are growing in the same environmental conditions; this is just one o...

Full description

Autores:
Arias Castro, Juan Carlos
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2017
Institución:
Universidad de los Andes
Repositorio:
Séneca: repositorio Uniandes
Idioma:
eng
OAI Identifier:
oai:repositorio.uniandes.edu.co:1992/61741
Acceso en línea:
http://hdl.handle.net/1992/61741
Palabra clave:
Células
Expresión génica
Fisiología celular
Microfluídica
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/4.0/
id UNIANDES2_1736d9f6afd365c44799bfb0c41d0eef
oai_identifier_str oai:repositorio.uniandes.edu.co:1992/61741
network_acronym_str UNIANDES2
network_name_str Séneca: repositorio Uniandes
repository_id_str
spelling Al consultar y hacer uso de este recurso, está aceptando las condiciones de uso establecidas por los autores.http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pedraza Leal, Juan Manuelvirtual::3894-1Arias Castro, Juan Carlos7723248b-5143-4101-95b4-15fdf24a33eb500El Karoui, MeriemForero Shelton, Antonio Manu2022-09-26T22:34:54Z2022-09-26T22:34:54Z2017http://hdl.handle.net/1992/6174110.57784/1992/61741instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/795488-1001"Interest in studies about variability in gene expression has been increasing due to its applications in pharmaceutical, medical and biological sciences. Genetically identical cells can have different phenotypes even when they are growing in the same environmental conditions; this is just one of many examples of the consequences of stochasticity in the chemical reactions at a molecular level. In our work we try to find insights on the sources of physiology and gene expression variability and their interaction with the environment, such as the case of nutrient limitation." -- Tomado del Formato de Documento de Grado."El interés en estudios sobre variabilidad en la expresión genética ha venido incrementando debido a sus aplicaciones en las industrias farmacéuticas, médicas y ciencias biológicas. Células genéticamente idénticas pueden tener diferentes fenotipos incluso cuando están creciendo en las mismas condiciones ambientales. Este es solo uno de muchos ejemplos de las consecuencias de la estocasticidad en las reacciones químicas a nivel molecular. En nuestro trabajo intentamos comprender las fuentes de variabilidad en fisiología y expresión genética, y su interacción con el ambiente como en el caso de limitación de nutrientes. Para lograr esto tomamos dos acercamientos. El primero es una técnica de microfluidos que cuantifica moléculas individuales dentro de células. La segunda, un experimento para explorar los cambios en la fisiología y expresión genética de las células mientras evolucionan en un medio con limitación de nutrientes." -- Tomado del Formato de Documento de Grado.Doctor en Ciencias - FísicaDoctorado142 hojasapplication/pdfengUniversidad de los AndesDoctorado en Ciencias - FísicaFacultad de CienciasDepartamento de FísicaPhysiology, evolution and single cell gene expression : an experimental approach using a microfluidics techniqueTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDCélulasExpresión génicaFisiología celularMicrofluídica200520073Publicationhttps://scholar.google.es/citations?user=x8-YWMsAAAAJvirtual::3894-10000-0002-1802-3337virtual::3894-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000001817virtual::3894-1a4c0056f-ab75-4234-9297-925380d7633avirtual::3894-1a4c0056f-ab75-4234-9297-925380d7633avirtual::3894-1TEXT13434.pdf.txt13434.pdf.txtExtracted texttext/plain309680https://repositorio.uniandes.edu.co/bitstreams/bea0d2a6-a8f5-4a79-b894-5b8aba69cf67/downloadd49e76ec5ba5cfccca20b94f6b5698faMD52THUMBNAIL13434.pdf.jpg13434.pdf.jpgIM Thumbnailimage/jpeg27006https://repositorio.uniandes.edu.co/bitstreams/47c633a3-5ee8-47d3-9d38-412a64be7c93/downloadbcf5884c2b35bf4bf064a34351e51f71MD53ORIGINAL13434.pdfapplication/pdf19360171https://repositorio.uniandes.edu.co/bitstreams/ee9bf988-eb33-4334-b488-29dcafc94e26/download1b4228624ac66c7abbe123c281ae2603MD511992/61741oai:repositorio.uniandes.edu.co:1992/617412024-08-26 15:21:29.0http://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://repositorio.uniandes.edu.coRepositorio institucional Sénecaadminrepositorio@uniandes.edu.co
dc.title.spa.fl_str_mv Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
title Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
spellingShingle Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
Células
Expresión génica
Fisiología celular
Microfluídica
title_short Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
title_full Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
title_fullStr Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
title_full_unstemmed Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
title_sort Physiology, evolution and single cell gene expression : an experimental approach using a microfluidics technique
dc.creator.fl_str_mv Arias Castro, Juan Carlos
dc.contributor.advisor.none.fl_str_mv Pedraza Leal, Juan Manuel
dc.contributor.author.none.fl_str_mv Arias Castro, Juan Carlos
dc.contributor.jury.none.fl_str_mv El Karoui, Meriem
Forero Shelton, Antonio Manu
dc.subject.keyword.spa.fl_str_mv Células
Expresión génica
Fisiología celular
Microfluídica
topic Células
Expresión génica
Fisiología celular
Microfluídica
description "Interest in studies about variability in gene expression has been increasing due to its applications in pharmaceutical, medical and biological sciences. Genetically identical cells can have different phenotypes even when they are growing in the same environmental conditions; this is just one of many examples of the consequences of stochasticity in the chemical reactions at a molecular level. In our work we try to find insights on the sources of physiology and gene expression variability and their interaction with the environment, such as the case of nutrient limitation." -- Tomado del Formato de Documento de Grado.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-09-26T22:34:54Z
dc.date.available.none.fl_str_mv 2022-09-26T22:34:54Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/1992/61741
dc.identifier.doi.none.fl_str_mv 10.57784/1992/61741
dc.identifier.instname.spa.fl_str_mv instname:Universidad de los Andes
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Séneca
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uniandes.edu.co/
dc.identifier.local.spa.fl_str_mv 795488-1001
url http://hdl.handle.net/1992/61741
identifier_str_mv 10.57784/1992/61741
instname:Universidad de los Andes
reponame:Repositorio Institucional Séneca
repourl:https://repositorio.uniandes.edu.co/
795488-1001
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 142 hojas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de los Andes
dc.publisher.program.spa.fl_str_mv Doctorado en Ciencias - Física
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.department.spa.fl_str_mv Departamento de Física
institution Universidad de los Andes
bitstream.url.fl_str_mv https://repositorio.uniandes.edu.co/bitstreams/bea0d2a6-a8f5-4a79-b894-5b8aba69cf67/download
https://repositorio.uniandes.edu.co/bitstreams/47c633a3-5ee8-47d3-9d38-412a64be7c93/download
https://repositorio.uniandes.edu.co/bitstreams/ee9bf988-eb33-4334-b488-29dcafc94e26/download
bitstream.checksum.fl_str_mv d49e76ec5ba5cfccca20b94f6b5698fa
bcf5884c2b35bf4bf064a34351e51f71
1b4228624ac66c7abbe123c281ae2603
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio institucional Séneca
repository.mail.fl_str_mv adminrepositorio@uniandes.edu.co
_version_ 1812133857709785088

Publicaciones similares