Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure
Zebrafish (Danio rerio) have emerged as one of the most widely used animal models, particularly for neurobiological and genetic high throughput studies. The aim of this study was to evaluate larval zebrafish responses to chronic stressors by using behavioral assays in an effort to characterize early...
- Autores:
-
Tamayo Montilla, Daniela
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2023
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/64550
- Acceso en línea:
- http://hdl.handle.net/1992/64550
- Palabra clave:
- Stress response
Anxious behavior
Early-life stress
Zebrafish larvae
Biología
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| title |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| spellingShingle |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure Stress response Anxious behavior Early-life stress Zebrafish larvae Biología |
| title_short |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| title_full |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| title_fullStr |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| title_full_unstemmed |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| title_sort |
Evaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposure |
| dc.creator.fl_str_mv |
Tamayo Montilla, Daniela |
| dc.contributor.advisor.none.fl_str_mv |
Bloch Morel, Natasha Ivonne Lattig Matiz, María Claudia |
| dc.contributor.author.none.fl_str_mv |
Tamayo Montilla, Daniela |
| dc.contributor.researchgroup.es_CO.fl_str_mv |
Neurogenética y Comportamiento (Departamento de Ingeniería Biomédica) Neurociencia y Ritmos Circadianos (Facultad de Medicina) |
| dc.subject.keyword.none.fl_str_mv |
Stress response Anxious behavior Early-life stress Zebrafish larvae |
| topic |
Stress response Anxious behavior Early-life stress Zebrafish larvae Biología |
| dc.subject.themes.es_CO.fl_str_mv |
Biología |
| description |
Zebrafish (Danio rerio) have emerged as one of the most widely used animal models, particularly for neurobiological and genetic high throughput studies. The aim of this study was to evaluate larval zebrafish responses to chronic stressors by using behavioral assays in an effort to characterize early life anxiogenic behavior, which might influence development of neuropsychiatric disorders later on. After establishing a chronic unknown early life stress (CUELS) protocol which did not affect survival rates, notable stress- related behaviors were identified for stressed larvae. These included significant freezing, erratic behaviors and preference for lighter environments, all of which were not identifiable before the protocol. In the future, these results will build into valuable insights in the context of post traumatic stress disorder (PTSD), since standardizing methods for efficient identification of anxious patterns during the first weeks of development is crucial considering the fact that distressing stimuli leading to development of this and other disorders could be presented during this time. |
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2023 |
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2023-02-02T15:24:20Z |
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2023-02-02T15:24:20Z |
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2023-02-01 |
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Trabajo de grado - Pregrado |
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info:eu-repo/semantics/bachelorThesis |
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eng |
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Ahmad, F. and Richardson, M. K. (2013) Exploratory behaviour in the open field test adapted for larval zebrafish: impact of environmental complexity. Behavioural processes, 92, 88-98. Bai, Y., Liu, H., Huang, B., Wagle, M. and Guo, S. (2016) Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC neuroscience, 17, 1-12. Basnet, R. M., Zizioli, D., Taweedet, S., Finazzi, D. and Memo, M. (2019) Zebrafish larvae as a behavioral model in neuropharmacology. Biomedicines, 7, 23. Brand, S. J., Botha, T. L. and Wepener, V. (2020) Behavioural response as a reliable measure of acute nanomaterial toxicity in zebrafish larvae exposed to a carbon-based versus a metal-based nanomaterial. African zoology, 55, 57-66. Brown, A. R., Owen, S. F., Peters, J., Zhang, Y., Soffker, M., Paull, G. C., Hosken, D. J., Wahab, M. A. and Tyler, C. R. (2015) Climate change and pollution speed declines in zebrafish populations. Proceedings of the National Academy of Sciences, 112, E1237-E1246. Carr, C. P., Martins, C. M. S., Stingel, A. M., Lemgruber, V. B. and Juruena, M. F. (2013) The role of early life stress in adult psychiatric disorders: a systematic review according to childhood trauma subtypes. The Journal of Nervous and Mental Disease, 201, 1007-1020. Charmandari, E., Tsigos, C. and Chrousos, G. (2005) Endocrinology of the stress response 1. Annual review of physiology, 67, 259-284. Chin, J. S., Phan, T.-A. N., Albert, L. T., Keene, A. C. and Duboué, E. R. (2021) Long lasting anxiety following early life stress is dependent on glucocorticoid signaling in zebrafish. bioRxiv. Clark, K. J., Boczek, N. J. and Ekker, S. C. (2011) Stressing zebrafish for behavioral genetics. 22, 49-62. URL: https://doi.org/10.1515/rns.2011.007. De Marco, R. J., Groneberg, A. H., Yeh, C.-M., Treviño, M. and Ryu, S. (2014) The behavior of larval zebrafish reveals stressor-mediated anorexia during early vertebrate development. Frontiers in behavioral neuroscience, 8, 367. Fontana, B. D., Gibbon, A. J., Cleal, M., Norton, W. H. and Parker, M. O. (2021) Chronic unpredictable early-life stress (CUELS) protocol: Early-life stress changes anxiety levels of adult zebrafish. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 108, 110087. Golla, A., Østby, H. and Kermen, F. (2020) Chronic unpredictable stress induces anxiety-like behaviors in young zebrafish. Scientific reports, 10, 1-10. Haney, W. A., Moussaoui, B. and Strother, J. A. (2020) Prolonged exposure to stressors suppresses exploratory behavior in zebrafish larvae. Journal of Experimental Biology, 223, jeb224964. Horn, S. R., Charney, D. S. and Feder, A. (2016) Understanding resilience: New approaches for preventing and treating PTSD. Experimental neurology, 284, 119-132. Kilaru, V., Iyer, S., Almli, L., Stevens, J., Lori, A., Jovanovic, T., Ely, T., Bradley, B., Binder, E., Koen, N. et al. (2016) Genome-wide gene-based analysis suggests an association between neuroligin 1 (nlgn1) and post-traumatic stress disorder. Translational psychiatry, 6, e820-e820. Legradi, J., El Abdellaoui, N., Van Pomeren, M. and Legler, J. (2015) Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity. Environmental Science and Pollution Research, 22, 16277-16289. Magruder, K. M. and Yeager, D. E. (2009) The prevalence of PTSD across war eras and the effect of deployment on PTSD: A systematic review and meta-analysis. Psychiatric Annals, 39. Maximino, C., de Brito, T. M., da Silva Batista, A. W., Herculano, A. M., Morato, S. and Gouveia Jr, A. (2010) Measuring anxiety in zebrafish: a critical review. Behavioural brain research, 214, 157-171. Robison, B. D., Benner, M. J., Singer, M. L. and Oswald, M. E. (2012) A high-throughput and inexpensive assay for anxiety-related behaviors in the zebrafish, based on place preference and latency to feed. In Zebrafish Protocols for Neurobehavioral Research, 203-215. Springer. Schiavone, S., Colaianna, M. and Curtis, L. (2015) Impact of early life stress on the pathogenesis of mental disorders: relation to brain oxidative stress. Current pharmaceutical design, 21, 1404-1412. Tegelenbosch, R. A., Noldus, L. P., Richardson, M. K. and Ahmad, F. (2012) Zebrafish embryos and larvae in behavioural assays. Behaviour, 149, 1241-1281. |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Bloch Morel, Natasha Ivonneb751f88a-6344-4f6c-b4c3-f4f1041d3a6e600Lattig Matiz, María Claudiavirtual::12835-1Tamayo Montilla, Daniela4e501e55-540a-4a1b-9f63-8157d86e7565600Neurogenética y Comportamiento (Departamento de Ingeniería Biomédica)Neurociencia y Ritmos Circadianos (Facultad de Medicina)2023-02-02T15:24:20Z2023-02-02T15:24:20Z2023-02-01http://hdl.handle.net/1992/64550instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Zebrafish (Danio rerio) have emerged as one of the most widely used animal models, particularly for neurobiological and genetic high throughput studies. The aim of this study was to evaluate larval zebrafish responses to chronic stressors by using behavioral assays in an effort to characterize early life anxiogenic behavior, which might influence development of neuropsychiatric disorders later on. After establishing a chronic unknown early life stress (CUELS) protocol which did not affect survival rates, notable stress- related behaviors were identified for stressed larvae. These included significant freezing, erratic behaviors and preference for lighter environments, all of which were not identifiable before the protocol. In the future, these results will build into valuable insights in the context of post traumatic stress disorder (PTSD), since standardizing methods for efficient identification of anxious patterns during the first weeks of development is crucial considering the fact that distressing stimuli leading to development of this and other disorders could be presented during this time.BiólogoPregradoEstrés y comportamiento8 páginasapplication/pdfengUniversidad de los AndesBiologíaFacultad de CienciasDepartamento de Ciencias BiológicasEvaluation of anxious behavior in larval zebrafish (Danio rerio) after early life chronic stress exposureTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPStress responseAnxious behaviorEarly-life stressZebrafish larvaeBiologíaAhmad, F. and Richardson, M. K. (2013) Exploratory behaviour in the open field test adapted for larval zebrafish: impact of environmental complexity. Behavioural processes, 92, 88-98.Bai, Y., Liu, H., Huang, B., Wagle, M. and Guo, S. (2016) Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC neuroscience, 17, 1-12.Basnet, R. M., Zizioli, D., Taweedet, S., Finazzi, D. and Memo, M. (2019) Zebrafish larvae as a behavioral model in neuropharmacology. Biomedicines, 7, 23.Brand, S. J., Botha, T. L. and Wepener, V. (2020) Behavioural response as a reliable measure of acute nanomaterial toxicity in zebrafish larvae exposed to a carbon-based versus a metal-based nanomaterial. African zoology, 55, 57-66.Brown, A. R., Owen, S. F., Peters, J., Zhang, Y., Soffker, M., Paull, G. C., Hosken, D. J., Wahab, M. A. and Tyler, C. R. (2015) Climate change and pollution speed declines in zebrafish populations. Proceedings of the National Academy of Sciences, 112, E1237-E1246.Carr, C. P., Martins, C. M. S., Stingel, A. M., Lemgruber, V. B. and Juruena, M. F. (2013) The role of early life stress in adult psychiatric disorders: a systematic review according to childhood trauma subtypes. The Journal of Nervous and Mental Disease, 201, 1007-1020.Charmandari, E., Tsigos, C. and Chrousos, G. (2005) Endocrinology of the stress response 1. Annual review of physiology, 67, 259-284.Chin, J. S., Phan, T.-A. N., Albert, L. T., Keene, A. C. and Duboué, E. R. (2021) Long lasting anxiety following early life stress is dependent on glucocorticoid signaling in zebrafish. bioRxiv.Clark, K. J., Boczek, N. J. and Ekker, S. C. (2011) Stressing zebrafish for behavioral genetics. 22, 49-62. URL: https://doi.org/10.1515/rns.2011.007.De Marco, R. J., Groneberg, A. H., Yeh, C.-M., Treviño, M. and Ryu, S. (2014) The behavior of larval zebrafish reveals stressor-mediated anorexia during early vertebrate development. Frontiers in behavioral neuroscience, 8, 367.Fontana, B. D., Gibbon, A. J., Cleal, M., Norton, W. H. and Parker, M. O. (2021) Chronic unpredictable early-life stress (CUELS) protocol: Early-life stress changes anxiety levels of adult zebrafish. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 108, 110087.Golla, A., Østby, H. and Kermen, F. (2020) Chronic unpredictable stress induces anxiety-like behaviors in young zebrafish. Scientific reports, 10, 1-10.Haney, W. A., Moussaoui, B. and Strother, J. A. (2020) Prolonged exposure to stressors suppresses exploratory behavior in zebrafish larvae. Journal of Experimental Biology, 223, jeb224964.Horn, S. R., Charney, D. S. and Feder, A. (2016) Understanding resilience: New approaches for preventing and treating PTSD. Experimental neurology, 284, 119-132.Kilaru, V., Iyer, S., Almli, L., Stevens, J., Lori, A., Jovanovic, T., Ely, T., Bradley, B., Binder, E., Koen, N. et al. (2016) Genome-wide gene-based analysis suggests an association between neuroligin 1 (nlgn1) and post-traumatic stress disorder. Translational psychiatry, 6, e820-e820.Legradi, J., El Abdellaoui, N., Van Pomeren, M. and Legler, J. (2015) Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity. Environmental Science and Pollution Research, 22, 16277-16289.Magruder, K. M. and Yeager, D. E. (2009) The prevalence of PTSD across war eras and the effect of deployment on PTSD: A systematic review and meta-analysis. Psychiatric Annals, 39.Maximino, C., de Brito, T. M., da Silva Batista, A. W., Herculano, A. M., Morato, S. and Gouveia Jr, A. (2010) Measuring anxiety in zebrafish: a critical review. Behavioural brain research, 214, 157-171.Robison, B. D., Benner, M. J., Singer, M. L. and Oswald, M. E. (2012) A high-throughput and inexpensive assay for anxiety-related behaviors in the zebrafish, based on place preference and latency to feed. In Zebrafish Protocols for Neurobehavioral Research, 203-215. Springer.Schiavone, S., Colaianna, M. and Curtis, L. (2015) Impact of early life stress on the pathogenesis of mental disorders: relation to brain oxidative stress. Current pharmaceutical design, 21, 1404-1412.Tegelenbosch, R. A., Noldus, L. P., Richardson, M. K. and Ahmad, F. (2012) Zebrafish embryos and larvae in behavioural assays. 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