Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus

This work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related pep...

Full description

Autores:
Martinez-Gutierrez, Marlen
Castellanos, Jaime
Tipo de recurso:
Fecha de publicación:
2007
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/1903
Acceso en línea:
http://hdl.handle.net/20.500.12495/1903
https://doi.org/10.1007/s00401-007-0222-9
Palabra clave:
Antígenos virales
Péptido intestinal vasoactivo
Neuropéptido
Neuropeptides
Rabies virus
Sensory neuron
Rights
License
Acceso cerrado
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oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/1903
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
title Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
spellingShingle Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
Antígenos virales
Péptido intestinal vasoactivo
Neuropéptido
Neuropeptides
Rabies virus
Sensory neuron
title_short Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
title_full Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
title_fullStr Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
title_full_unstemmed Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
title_sort Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
dc.creator.fl_str_mv Martinez-Gutierrez, Marlen
Castellanos, Jaime
dc.contributor.author.none.fl_str_mv Martinez-Gutierrez, Marlen
Castellanos, Jaime
dc.contributor.orcid.none.fl_str_mv Castellanos, Jaime [0000-0003-1596-8383]
dc.subject.decs.spa.fl_str_mv Antígenos virales
Péptido intestinal vasoactivo
Neuropéptido
topic Antígenos virales
Péptido intestinal vasoactivo
Neuropéptido
Neuropeptides
Rabies virus
Sensory neuron
dc.subject.keywords.spa.fl_str_mv Neuropeptides
Rabies virus
Sensory neuron
description This work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related peptide (CGRP), galanin (GAL), substance P (SP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). It was found that 56% of the neurons in culture were small (diameter <20 m) but, in spite of this, 69% of the infected neurons had intermediate and large diameters (̧20 m). More than 50% of infected neurons expressed NPY, VIP or SP, whereas no association was found between infected neurons and the presence of CGRP or GAL. Despite SP having been shown to be a small neuron marker, it was found that RABV infects medium and large-sized SP positive cells. RABV preference for larger neurons could explain part of the neuropathogenesis since it can be suggested that, following a rabid accident, the virus uses large neurons (mainly innervating muscle and joints) in vivo to be transported later on to the central nervous system.
publishDate 2007
dc.date.issued.none.fl_str_mv 2007
dc.date.accessioned.none.fl_str_mv 2020-01-22T21:07:18Z
dc.date.available.none.fl_str_mv 2020-01-22T21:07:18Z
dc.type.spa.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.local.spa.fl_str_mv artículo
dc.identifier.issn.none.fl_str_mv 0001-6322
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/1903
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s00401-007-0222-9
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv repourl:https://repositorio.unbosque.edu.co
identifier_str_mv 0001-6322
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
repourl:https://repositorio.unbosque.edu.co
url http://hdl.handle.net/20.500.12495/1903
https://doi.org/10.1007/s00401-007-0222-9
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Acta neuropathologica, 0001-6322, Nro.114, 2007, p. 263-269
dc.relation.uri.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs00401-007-0222-9
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dc.rights.local.spa.fl_str_mv Acceso cerrado
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf210
dc.rights.creativecommons.none.fl_str_mv 2007
rights_invalid_str_mv Acceso cerrado
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2007
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer Verlag
dc.publisher.journal.spa.fl_str_mv Acta neuropathologica
institution Universidad El Bosque
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spelling Martinez-Gutierrez, MarlenCastellanos, JaimeCastellanos, Jaime [0000-0003-1596-8383]2020-01-22T21:07:18Z2020-01-22T21:07:18Z20070001-6322http://hdl.handle.net/20.500.12495/1903https://doi.org/10.1007/s00401-007-0222-9instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengSpringer VerlagActa neuropathologicaActa neuropathologica, 0001-6322, Nro.114, 2007, p. 263-269https://link.springer.com/article/10.1007%2Fs00401-007-0222-9Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virusarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Antígenos viralesPéptido intestinal vasoactivoNeuropéptidoNeuropeptidesRabies virusSensory neuronThis work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related peptide (CGRP), galanin (GAL), substance P (SP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). It was found that 56% of the neurons in culture were small (diameter <20 m) but, in spite of this, 69% of the infected neurons had intermediate and large diameters (̧20 m). More than 50% of infected neurons expressed NPY, VIP or SP, whereas no association was found between infected neurons and the presence of CGRP or GAL. Despite SP having been shown to be a small neuron marker, it was found that RABV infects medium and large-sized SP positive cells. RABV preference for larger neurons could explain part of the neuropathogenesis since it can be suggested that, following a rabid accident, the virus uses large neurons (mainly innervating muscle and joints) in vivo to be transported later on to the central nervous system.Acceso cerradohttp://purl.org/coar/access_right/c_abf2102007http://purl.org/coar/access_right/c_abf2THUMBNAILMartínez, M., Castellanos, J._2007.jpgMartínez, M., Castellanos, J._2007.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/5228fa6c-e274-46bc-9063-e2f5315a7c2c/download7210a811635d1799e7c05fee5d259be7MD53Martínez, M., Castellanos, J._2007.pdf.jpgMartínez, M., Castellanos, J._2007.pdf.jpgIM Thumbnailimage/jpeg9141https://repositorio.unbosque.edu.co/bitstreams/2d8ef0fd-5df0-4f7f-8869-c9a9a2b521a4/download35003a6fb7ec45e096c6f431477439e2MD54ORIGINALMartínez, M., Castellanos, J._2007.pdfMartínez, M., Castellanos, J._2007.pdfapplication/pdf420170https://repositorio.unbosque.edu.co/bitstreams/67f19716-b90b-478b-ab4f-98028eda9ad9/downloadb897d630ff38a55f5b73bbc3b88b4cc8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/7f5961e0-f829-4afa-ae28-212ae4a162f5/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMartínez, M., Castellanos, J._2007.pdf.txtMartínez, M., Castellanos, J._2007.pdf.txtExtracted texttext/plain38228https://repositorio.unbosque.edu.co/bitstreams/96f250cc-a71f-41a2-8d51-ed64fece58be/downloadb1b9a42b8f1d133a105cf1bb95df413dMD5520.500.12495/1903oai:repositorio.unbosque.edu.co:20.500.12495/19032024-02-06 22:49:01.643restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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