Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus
This work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related pep...
- Autores:
-
Martinez-Gutierrez, Marlen
Castellanos, Jaime
- Tipo de recurso:
- Fecha de publicación:
- 2007
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/1903
- Palabra clave:
- Antígenos virales
Péptido intestinal vasoactivo
Neuropéptido
Neuropeptides
Rabies virus
Sensory neuron
- Rights
- License
- Acceso cerrado
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oai:repositorio.unbosque.edu.co:20.500.12495/1903 |
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UNBOSQUE2 |
network_name_str |
Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
title |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
spellingShingle |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus Antígenos virales Péptido intestinal vasoactivo Neuropéptido Neuropeptides Rabies virus Sensory neuron |
title_short |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
title_full |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
title_fullStr |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
title_full_unstemmed |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
title_sort |
Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virus |
dc.creator.fl_str_mv |
Martinez-Gutierrez, Marlen Castellanos, Jaime |
dc.contributor.author.none.fl_str_mv |
Martinez-Gutierrez, Marlen Castellanos, Jaime |
dc.contributor.orcid.none.fl_str_mv |
Castellanos, Jaime [0000-0003-1596-8383] |
dc.subject.decs.spa.fl_str_mv |
Antígenos virales Péptido intestinal vasoactivo Neuropéptido |
topic |
Antígenos virales Péptido intestinal vasoactivo Neuropéptido Neuropeptides Rabies virus Sensory neuron |
dc.subject.keywords.spa.fl_str_mv |
Neuropeptides Rabies virus Sensory neuron |
description |
This work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related peptide (CGRP), galanin (GAL), substance P (SP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). It was found that 56% of the neurons in culture were small (diameter <20 m) but, in spite of this, 69% of the infected neurons had intermediate and large diameters (̧20 m). More than 50% of infected neurons expressed NPY, VIP or SP, whereas no association was found between infected neurons and the presence of CGRP or GAL. Despite SP having been shown to be a small neuron marker, it was found that RABV infects medium and large-sized SP positive cells. RABV preference for larger neurons could explain part of the neuropathogenesis since it can be suggested that, following a rabid accident, the virus uses large neurons (mainly innervating muscle and joints) in vivo to be transported later on to the central nervous system. |
publishDate |
2007 |
dc.date.issued.none.fl_str_mv |
2007 |
dc.date.accessioned.none.fl_str_mv |
2020-01-22T21:07:18Z |
dc.date.available.none.fl_str_mv |
2020-01-22T21:07:18Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.local.spa.fl_str_mv |
artículo |
dc.identifier.issn.none.fl_str_mv |
0001-6322 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/1903 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s00401-007-0222-9 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
0001-6322 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/1903 https://doi.org/10.1007/s00401-007-0222-9 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Acta neuropathologica, 0001-6322, Nro.114, 2007, p. 263-269 |
dc.relation.uri.none.fl_str_mv |
https://link.springer.com/article/10.1007%2Fs00401-007-0222-9 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.local.spa.fl_str_mv |
Acceso cerrado |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf210 |
dc.rights.creativecommons.none.fl_str_mv |
2007 |
rights_invalid_str_mv |
Acceso cerrado http://purl.org/coar/access_right/c_abf210 2007 http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer Verlag |
dc.publisher.journal.spa.fl_str_mv |
Acta neuropathologica |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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Martinez-Gutierrez, MarlenCastellanos, JaimeCastellanos, Jaime [0000-0003-1596-8383]2020-01-22T21:07:18Z2020-01-22T21:07:18Z20070001-6322http://hdl.handle.net/20.500.12495/1903https://doi.org/10.1007/s00401-007-0222-9instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengSpringer VerlagActa neuropathologicaActa neuropathologica, 0001-6322, Nro.114, 2007, p. 263-269https://link.springer.com/article/10.1007%2Fs00401-007-0222-9Morphological and biochemical characterisation of sensory neurons infected in vitro with rabies virusarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Antígenos viralesPéptido intestinal vasoactivoNeuropéptidoNeuropeptidesRabies virusSensory neuronThis work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection. Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related peptide (CGRP), galanin (GAL), substance P (SP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). It was found that 56% of the neurons in culture were small (diameter <20 m) but, in spite of this, 69% of the infected neurons had intermediate and large diameters (̧20 m). More than 50% of infected neurons expressed NPY, VIP or SP, whereas no association was found between infected neurons and the presence of CGRP or GAL. Despite SP having been shown to be a small neuron marker, it was found that RABV infects medium and large-sized SP positive cells. RABV preference for larger neurons could explain part of the neuropathogenesis since it can be suggested that, following a rabid accident, the virus uses large neurons (mainly innervating muscle and joints) in vivo to be transported later on to the central nervous system.Acceso cerradohttp://purl.org/coar/access_right/c_abf2102007http://purl.org/coar/access_right/c_abf2THUMBNAILMartínez, M., Castellanos, J._2007.jpgMartínez, M., Castellanos, J._2007.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/5228fa6c-e274-46bc-9063-e2f5315a7c2c/download7210a811635d1799e7c05fee5d259be7MD53Martínez, M., Castellanos, J._2007.pdf.jpgMartínez, M., Castellanos, J._2007.pdf.jpgIM Thumbnailimage/jpeg9141https://repositorio.unbosque.edu.co/bitstreams/2d8ef0fd-5df0-4f7f-8869-c9a9a2b521a4/download35003a6fb7ec45e096c6f431477439e2MD54ORIGINALMartínez, M., Castellanos, J._2007.pdfMartínez, M., Castellanos, J._2007.pdfapplication/pdf420170https://repositorio.unbosque.edu.co/bitstreams/67f19716-b90b-478b-ab4f-98028eda9ad9/downloadb897d630ff38a55f5b73bbc3b88b4cc8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/7f5961e0-f829-4afa-ae28-212ae4a162f5/download8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMartínez, M., Castellanos, J._2007.pdf.txtMartínez, M., Castellanos, J._2007.pdf.txtExtracted texttext/plain38228https://repositorio.unbosque.edu.co/bitstreams/96f250cc-a71f-41a2-8d51-ed64fece58be/downloadb1b9a42b8f1d133a105cf1bb95df413dMD5520.500.12495/1903oai:repositorio.unbosque.edu.co:20.500.12495/19032024-02-06 22:49:01.643restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |