Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
Neurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and afte...
- Autores:
-
Castellanos, Jaime
Martínez-Gutiérrez, Marlén
Hurtado, Hernán
Kassis, Raïd
Bourhy, Hervé
Acosta, Orlando
Lafon, Monique
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2005
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/5420
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/5420
https://doi.org/10.1080/13550280500187252
https://repositorio.unbosque.edu.co
- Palabra clave:
- Antiviral effect
Neurotrophins
Rabies
Real time PCR
Sensory neuron
- Rights
- openAccess
- License
- Acceso abierto
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UNBOSQUE2 |
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Repositorio U. El Bosque |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
dc.title.translated.spa.fl_str_mv |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
title |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
spellingShingle |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures Antiviral effect Neurotrophins Rabies Real time PCR Sensory neuron |
title_short |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
title_full |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
title_fullStr |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
title_full_unstemmed |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
title_sort |
Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures |
dc.creator.fl_str_mv |
Castellanos, Jaime Martínez-Gutiérrez, Marlén Hurtado, Hernán Kassis, Raïd Bourhy, Hervé Acosta, Orlando Lafon, Monique |
dc.contributor.author.none.fl_str_mv |
Castellanos, Jaime Martínez-Gutiérrez, Marlén Hurtado, Hernán Kassis, Raïd Bourhy, Hervé Acosta, Orlando Lafon, Monique |
dc.subject.keywords.spa.fl_str_mv |
Antiviral effect Neurotrophins Rabies Real time PCR Sensory neuron |
topic |
Antiviral effect Neurotrophins Rabies Real time PCR Sensory neuron |
description |
Neurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and after withdrawing the viral inoculum (post-treatment). NT pretreatment for 4 days prior to infection produced a significant increase in the quantity of virus adsorbed into cultures and a concomitant increase in genomic viral RNA as measured by real time polymerase chain reaction (PCR). NT pretreatment triggered increased expression of two rabies virus receptors (NCAM and p75NTR); however, no increase in rabies virus transcription and expression could be observed. By contrast, NT treatment during and after infection (trans- and post-treatment) induced a strong decrease in the quantity of viral nucleoprotein genomic and messenger nucleoprotein RNAs. These findings suggested that NT had an intrinsic inhibitory effect on rabies virus infection, which was not counterbalanced by NTs’ rabies virus receptor—enhancing property and viral uptake. Adult mouse dorsal root ganglion cultures can be regarded as being a useful model for detecting therapeutic targets and evaluating experimental antiviral drugs. |
publishDate |
2005 |
dc.date.issued.none.fl_str_mv |
2005 |
dc.date.accessioned.none.fl_str_mv |
2021-02-23T16:13:38Z |
dc.date.available.none.fl_str_mv |
2021-02-23T16:13:38Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.issn.none.fl_str_mv |
1538-2443 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/5420 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1080/13550280500187252 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.unbosque.edu.co |
identifier_str_mv |
1538-2443 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque |
url |
http://hdl.handle.net/20.500.12495/5420 https://doi.org/10.1080/13550280500187252 https://repositorio.unbosque.edu.co |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Journal of neuroVirology, 1538-2443, Vol. 11, Nor. 4, 2005, p. 403-410 |
dc.relation.uri.none.fl_str_mv |
https://link.springer.com/article/10.1080/13550280500187252 |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess Acceso abierto |
dc.rights.creativecommons.none.fl_str_mv |
2005-07 |
rights_invalid_str_mv |
Acceso abierto http://purl.org/coar/access_right/c_abf2 2005-07 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer Nature |
dc.publisher.journal.spa.fl_str_mv |
Journal of neuroVirology |
institution |
Universidad El Bosque |
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spelling |
Castellanos, JaimeMartínez-Gutiérrez, MarlénHurtado, HernánKassis, RaïdBourhy, HervéAcosta, OrlandoLafon, Monique2021-02-23T16:13:38Z2021-02-23T16:13:38Z20051538-2443http://hdl.handle.net/20.500.12495/5420https://doi.org/10.1080/13550280500187252instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringer NatureJournal of neuroVirologyJournal of neuroVirology, 1538-2443, Vol. 11, Nor. 4, 2005, p. 403-410https://link.springer.com/article/10.1080/13550280500187252Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesStudying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Antiviral effectNeurotrophinsRabiesReal time PCRSensory neuronNeurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and after withdrawing the viral inoculum (post-treatment). NT pretreatment for 4 days prior to infection produced a significant increase in the quantity of virus adsorbed into cultures and a concomitant increase in genomic viral RNA as measured by real time polymerase chain reaction (PCR). NT pretreatment triggered increased expression of two rabies virus receptors (NCAM and p75NTR); however, no increase in rabies virus transcription and expression could be observed. By contrast, NT treatment during and after infection (trans- and post-treatment) induced a strong decrease in the quantity of viral nucleoprotein genomic and messenger nucleoprotein RNAs. These findings suggested that NT had an intrinsic inhibitory effect on rabies virus infection, which was not counterbalanced by NTs’ rabies virus receptor—enhancing property and viral uptake. Adult mouse dorsal root ganglion cultures can be regarded as being a useful model for detecting therapeutic targets and evaluating experimental antiviral drugs.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2005-07ORIGINALCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdfCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdfapplication/pdf282009https://repositorio.unbosque.edu.co/bitstreams/99e608cb-b5ca-4746-82ff-3b6a1d3ec0b6/download90955646f1a79c5b70d0d87ca98fc7a6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/a5e13c06-c84c-4e7b-88ec-47dceb76ee22/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.jpgCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/a4783f76-911e-4dfd-82b7-a724422c320e/download7210a811635d1799e7c05fee5d259be7MD53TEXTCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.txtCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.txtExtracted texttext/plain66169https://repositorio.unbosque.edu.co/bitstreams/c0488b42-9be5-4e3a-900a-13d7506d1253/downloada8915a2219c7cbe0b7579b87726115adMD5420.500.12495/5420oai:repositorio.unbosque.edu.co:20.500.12495/54202024-02-07 00:00:47.928restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |