Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures

Neurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and afte...

Full description

Autores:
Castellanos, Jaime
Martínez-Gutiérrez, Marlén
Hurtado, Hernán
Kassis, Raïd
Bourhy, Hervé
Acosta, Orlando
Lafon, Monique
Tipo de recurso:
Article of journal
Fecha de publicación:
2005
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/5420
Acceso en línea:
http://hdl.handle.net/20.500.12495/5420
https://doi.org/10.1080/13550280500187252
https://repositorio.unbosque.edu.co
Palabra clave:
Antiviral effect
Neurotrophins
Rabies
Real time PCR
Sensory neuron
Rights
openAccess
License
Acceso abierto
id UNBOSQUE2_ed096cd2c9ac90cd9c3d591073ab776d
oai_identifier_str oai:repositorio.unbosque.edu.co:20.500.12495/5420
network_acronym_str UNBOSQUE2
network_name_str Repositorio U. El Bosque
repository_id_str
dc.title.spa.fl_str_mv Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
dc.title.translated.spa.fl_str_mv Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
title Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
spellingShingle Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
Antiviral effect
Neurotrophins
Rabies
Real time PCR
Sensory neuron
title_short Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
title_full Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
title_fullStr Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
title_full_unstemmed Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
title_sort Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures
dc.creator.fl_str_mv Castellanos, Jaime
Martínez-Gutiérrez, Marlén
Hurtado, Hernán
Kassis, Raïd
Bourhy, Hervé
Acosta, Orlando
Lafon, Monique
dc.contributor.author.none.fl_str_mv Castellanos, Jaime
Martínez-Gutiérrez, Marlén
Hurtado, Hernán
Kassis, Raïd
Bourhy, Hervé
Acosta, Orlando
Lafon, Monique
dc.subject.keywords.spa.fl_str_mv Antiviral effect
Neurotrophins
Rabies
Real time PCR
Sensory neuron
topic Antiviral effect
Neurotrophins
Rabies
Real time PCR
Sensory neuron
description Neurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and after withdrawing the viral inoculum (post-treatment). NT pretreatment for 4 days prior to infection produced a significant increase in the quantity of virus adsorbed into cultures and a concomitant increase in genomic viral RNA as measured by real time polymerase chain reaction (PCR). NT pretreatment triggered increased expression of two rabies virus receptors (NCAM and p75NTR); however, no increase in rabies virus transcription and expression could be observed. By contrast, NT treatment during and after infection (trans- and post-treatment) induced a strong decrease in the quantity of viral nucleoprotein genomic and messenger nucleoprotein RNAs. These findings suggested that NT had an intrinsic inhibitory effect on rabies virus infection, which was not counterbalanced by NTs’ rabies virus receptor—enhancing property and viral uptake. Adult mouse dorsal root ganglion cultures can be regarded as being a useful model for detecting therapeutic targets and evaluating experimental antiviral drugs.
publishDate 2005
dc.date.issued.none.fl_str_mv 2005
dc.date.accessioned.none.fl_str_mv 2021-02-23T16:13:38Z
dc.date.available.none.fl_str_mv 2021-02-23T16:13:38Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.none.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 1538-2443
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12495/5420
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1080/13550280500187252
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
dc.identifier.repourl.none.fl_str_mv https://repositorio.unbosque.edu.co
identifier_str_mv 1538-2443
instname:Universidad El Bosque
reponame:Repositorio Institucional Universidad El Bosque
url http://hdl.handle.net/20.500.12495/5420
https://doi.org/10.1080/13550280500187252
https://repositorio.unbosque.edu.co
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Journal of neuroVirology, 1538-2443, Vol. 11, Nor. 4, 2005, p. 403-410
dc.relation.uri.none.fl_str_mv https://link.springer.com/article/10.1080/13550280500187252
dc.rights.local.spa.fl_str_mv Acceso abierto
dc.rights.accessrights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
Acceso abierto
dc.rights.creativecommons.none.fl_str_mv 2005-07
rights_invalid_str_mv Acceso abierto
http://purl.org/coar/access_right/c_abf2
2005-07
eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer Nature
dc.publisher.journal.spa.fl_str_mv Journal of neuroVirology
institution Universidad El Bosque
bitstream.url.fl_str_mv https://repositorio.unbosque.edu.co/bitstreams/99e608cb-b5ca-4746-82ff-3b6a1d3ec0b6/download
https://repositorio.unbosque.edu.co/bitstreams/a5e13c06-c84c-4e7b-88ec-47dceb76ee22/download
https://repositorio.unbosque.edu.co/bitstreams/a4783f76-911e-4dfd-82b7-a724422c320e/download
https://repositorio.unbosque.edu.co/bitstreams/c0488b42-9be5-4e3a-900a-13d7506d1253/download
bitstream.checksum.fl_str_mv 90955646f1a79c5b70d0d87ca98fc7a6
8a4605be74aa9ea9d79846c1fba20a33
7210a811635d1799e7c05fee5d259be7
a8915a2219c7cbe0b7579b87726115ad
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad El Bosque
repository.mail.fl_str_mv bibliotecas@biteca.com
_version_ 1814100718281293824
spelling Castellanos, JaimeMartínez-Gutiérrez, MarlénHurtado, HernánKassis, RaïdBourhy, HervéAcosta, OrlandoLafon, Monique2021-02-23T16:13:38Z2021-02-23T16:13:38Z20051538-2443http://hdl.handle.net/20.500.12495/5420https://doi.org/10.1080/13550280500187252instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquehttps://repositorio.unbosque.edu.coapplication/pdfengSpringer NatureJournal of neuroVirologyJournal of neuroVirology, 1538-2443, Vol. 11, Nor. 4, 2005, p. 403-410https://link.springer.com/article/10.1080/13550280500187252Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesStudying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Antiviral effectNeurotrophinsRabiesReal time PCRSensory neuronNeurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and after withdrawing the viral inoculum (post-treatment). NT pretreatment for 4 days prior to infection produced a significant increase in the quantity of virus adsorbed into cultures and a concomitant increase in genomic viral RNA as measured by real time polymerase chain reaction (PCR). NT pretreatment triggered increased expression of two rabies virus receptors (NCAM and p75NTR); however, no increase in rabies virus transcription and expression could be observed. By contrast, NT treatment during and after infection (trans- and post-treatment) induced a strong decrease in the quantity of viral nucleoprotein genomic and messenger nucleoprotein RNAs. These findings suggested that NT had an intrinsic inhibitory effect on rabies virus infection, which was not counterbalanced by NTs’ rabies virus receptor—enhancing property and viral uptake. Adult mouse dorsal root ganglion cultures can be regarded as being a useful model for detecting therapeutic targets and evaluating experimental antiviral drugs.Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessAcceso abierto2005-07ORIGINALCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdfCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdfapplication/pdf282009https://repositorio.unbosque.edu.co/bitstreams/99e608cb-b5ca-4746-82ff-3b6a1d3ec0b6/download90955646f1a79c5b70d0d87ca98fc7a6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/a5e13c06-c84c-4e7b-88ec-47dceb76ee22/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.jpgCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.jpgimage/jpeg5775https://repositorio.unbosque.edu.co/bitstreams/a4783f76-911e-4dfd-82b7-a724422c320e/download7210a811635d1799e7c05fee5d259be7MD53TEXTCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.txtCastellanos, J.E., Martínez-Gutierrez, M., Hurtado, H_2020.pdf.txtExtracted texttext/plain66169https://repositorio.unbosque.edu.co/bitstreams/c0488b42-9be5-4e3a-900a-13d7506d1253/downloada8915a2219c7cbe0b7579b87726115adMD5420.500.12495/5420oai:repositorio.unbosque.edu.co:20.500.12495/54202024-02-07 00:00:47.928restrictedhttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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